Microscopy Overview and Techniques
15 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Robert Hooke published treatise 'Micrographia' using a simple ______.

microscope

The objective lens of a compound microscope projects a magnified image into the ______ tube.

body

Bright Field Microscope produces a dark image to a light ______.

background

Total magnification of a microscope is calculated by multiplying the eyepiece by the ______.

<p>objective</p> Signup and view all the answers

Most commercial magnifiers produce a magnification of x2 to ______.

<p>30</p> Signup and view all the answers

The ______ lens magnifies the image formed by the objective lens.

<p>ocular</p> Signup and view all the answers

The ______ holds the objectives of the microscope.

<p>nosepiece</p> Signup and view all the answers

The distance between two front surfaces of the lens and the specimen is called the ______.

<p>working distance</p> Signup and view all the answers

The ______ controls the intensity of light produced.

<p>rheostat</p> Signup and view all the answers

The ______ microscope does not allow light to pass directly through the specimen.

<p>dark field</p> Signup and view all the answers

The technique that uses fluorescent stains is known as ______ microscopy.

<p>fluorescence</p> Signup and view all the answers

An opaque disk with a thin transparent ring is called an ______ stop in phase-contrast microscopy.

<p>annular</p> Signup and view all the answers

The numerical aperture (NA) describes the ______ gathering ability of the lens.

<p>light</p> Signup and view all the answers

The ______ microscope uses magnets to focus beams on the object being viewed.

<p>electron</p> Signup and view all the answers

Ernst Abbe formulated the ______ sine equation.

<p>Abbe</p> Signup and view all the answers

Study Notes

Microscopy Overview

  • Robert Hooke published "Micrographia," a pioneering treatise on microscopy.
  • Simple microscopes have a single bi-convex lens, primarily for dissection and observing bacterial colonies.
  • Limitations of simple microscopes include low amplification (x2 to 30) and resolution (around 10 μm) due to low Numerical Aperture (NA).

Compound Microscope

  • First developed by Zaccharias and Hans Janssen in 1590.
  • Features two lenses: an objective lens near the specimen and an ocular lens near the eye, allowing for two-stage magnification.
  • Significantly enhances viewing capabilities compared to simple microscopes.

Bright Field Microscope

  • Produces dark images against a light background through visible light transmission.
  • Total magnification calculated as eyepiece magnification multiplied by the objective’s power (e.g., 10x eyepiece and 100x objective yields 1000x).
  • Key components include:
    • Ocular Lens: Magnifies the objective's image.
    • Body Tube: Transmits images to the ocular.
    • Arm: Supports the microscope.
    • Nosepiece: Holds objectives.
    • Objective Lenses: Primary magnifying lenses.
    • Mechanical Stage: Holds slides in place.
    • Condenser: Focuses light on the specimen.
    • Diaphragm & Rheostat: Control light intensity and amount.
    • Adjustment Knobs: Coarse adjusts under low power; fine sharpens under all objectives.
    • Illuminator: Provides necessary light.

Resolution and Numerical Aperture

  • Resolution defined as the detail fidelity in magnified images.
  • Ernst Abbe formulated the Abbe sine equation: d = 0.5λ/(n sin θ).
  • Shorter wavelengths (450-500 nm) yield higher resolution, crucial for distinguishing close objects.
  • NA indicates a lens's light-gathering ability; higher NA correlates to lower working distance.

Objective Lens Characteristics

  • Scanning: 4x, NA 0.10, color red.
  • Low Power Objective (LPO): 10x, NA 0.25, color yellow.
  • High Power Objective (HPO): 40x, NA 0.65, color blue.
  • Oil Immersion: 100x, NA 1.25, color white.

Dark Field Microscope

  • Functions oppositely to bright field; light enters at oblique angles.
  • Ideal for viewing spirochetes and other unstained microorganisms.

Phase-Contrast Microscope

  • Designed for samples with low refractive indices, visualizing organelles without staining.
  • Produces bright backgrounds with dark specimen images due to light beam deflection.
  • Utilizes an annular stop condenser to create a hollow cone of light.

Fluorescence Microscope

  • Employs fluorochromes that fluoresce under UV or visible light exposure.
  • Common fluorochromes include acridine orange, auramine, and FITC.
  • Converts radiant energy in molecules to fluorescence, used for detailed visualization.

Electron Microscope

  • Utilizes electrons instead of light, capable of magnifying significantly smaller objects.
  • Operates in a vacuum to prevent interference from air molecules.
  • Produces an image via a monitor, allowing for high-resolution observation.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

Description

This quiz explores the fundamentals of microscopy, including the history and development of simple and compound microscopes. It also covers the principles of bright field microscopy, magnification calculations, and the key components involved. Test your knowledge on how these microscopes enhance our ability to observe microscopic specimens.

More Like This

Use Quizgecko on...
Browser
Browser