Microbiology Chapter 3: Observing Microorganisms Through a Microscope Quiz
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Questions and Answers

Which of the following is NOT a kind of staining technique?

  • Selective
  • Differential
  • Special (correct)
  • Simple
  • What is the purpose of fixing a slide in microbiology?

  • Staining the organisms
  • Killing the organisms (correct)
  • Illuminating the organisms
  • Magnifying the organisms
  • What is it called when colorless bacteria are prepared against a colored background?

  • Acid-fast staining
  • Gram staining
  • Endospore staining
  • Negative staining (correct)
  • In the Gram stain, what is the function of the mordant?

    <p>Iodine</p> Signup and view all the answers

    Which dye is commonly used to stain flagella in microbiology?

    <p>Malachite green</p> Signup and view all the answers

    What is the function of the diaphragm on a compound microscope?

    <p>Controls the amount of light entering the condenser</p> Signup and view all the answers

    What is the function of the ocular lens in microscopy?

    <p>To remagnify the image formed by the objective lens</p> Signup and view all the answers

    Which part of the microscope measures the light-bending ability of a medium?

    <p>Refractive index</p> Signup and view all the answers

    In microscopy, what does the resolution refer to?

    <p>Ability to distinguish between two points</p> Signup and view all the answers

    What would happen if you adjust the diaphragm to its smallest opening on a compound microscope?

    <p>The specimen will appear dimmer</p> Signup and view all the answers

    What effect does changing the refractive index of a medium have on microscopy?

    <p>Affects light bending in the medium</p> Signup and view all the answers

    If the ocular lens has a high magnification power, what impact does this have on the overall magnification in microscopy?

    <p>Has no effect on overall magnification</p> Signup and view all the answers

    Why is it important to properly clean and maintain microscope lenses regularly?

    <p>To avoid contamination of specimens</p> Signup and view all the answers

    Which microscope illuminates specimens with blue light and produces three-dimensional images?

    <p>Confocal microscope</p> Signup and view all the answers

    What microscope forms an image from two sets of light rays, one from the light source and the other diffracted from a structure in the specimen?

    <p>Phase-contrast microscope</p> Signup and view all the answers

    Which microscope uses a metal and diamond probe to produce a three-dimensional image?

    <p>Atomic force microscope</p> Signup and view all the answers

    What type of microscopes illuminates specimens with blue light and produces three-dimensional images?

    <p>Light microscope</p> Signup and view all the answers

    Which microscope utilizes a technique where only the scattered light reaches the objective lens to visualize unstained specimens?

    <p>Darkfield microscope</p> Signup and view all the answers

    What microscope would be most useful in observing fine structures like cell organelles?

    <p>Transmission electron microscope</p> Signup and view all the answers

    Which type of microscopy is commonly used for live-cell imaging due to its ability to provide optical sections of specimens?

    <p>Fluorescence microscopy</p> Signup and view all the answers

    Microscopy that is useful for viewing transparent specimens without staining?

    <p>Phase-Contrast Microscopy</p> Signup and view all the answers

    Which microscope is best suited for studying the surface structures of intact cells and viruses?

    <p>Scanning electron microscope</p> Signup and view all the answers

    In transmission electron microscopy, where is the specimen typically placed?

    <p>Copper mesh grid</p> Signup and view all the answers

    Which microscope is valuable for examining different layers of specimens and commonly involves shadow casting?

    <p>Transmission electron microscope</p> Signup and view all the answers

    What is the primary application of a phase-contrast microscope?

    <p>Enhancing contrast in transparent samples</p> Signup and view all the answers

    Which microscope is most suitable for detailed examination of the three-dimensional surface characteristics of a specimen?

    <p>Scanning electron microscope</p> Signup and view all the answers

    What distinguishes a darkfield microscope from other types when observing specimens?

    <p>It illuminates the specimen against a dark background</p> Signup and view all the answers

    How does a scanning electron microscope differ from a transmission electron microscope?

    <p>It uses electrons to scan specimens on the surface vs. through specimens</p> Signup and view all the answers

    What advantage does a phase-contrast microscope offer when examining transparent samples?

    <p>Allows imaging without staining techniques</p> Signup and view all the answers

    How are flagella stained?

    <p>Carbolfuchsin</p> Signup and view all the answers

    What stain is used to visualize capsules?

    <p>Schaeffer-Fulton stain</p> Signup and view all the answers

    Which staining method is employed to observe endospores?

    <p>Schaeffer-Fulton stain</p> Signup and view all the answers

    At the completion of the Gram staining process, what color are gram-negative bacteria?

    <p>Pink</p> Signup and view all the answers

    How does Mycobacterium leprae appear after staining with an acid-fast stain?

    <p>Red</p> Signup and view all the answers

    What color are gram-negative bacteria at the completion of the Gram staining process?

    <p>Orange</p> Signup and view all the answers

    Which type of staining is NOT used to visualize capsules?

    <p>Endospore stain</p> Signup and view all the answers

    What color are gram-positive bacteria at the end of Gram staining?

    <p>Purple</p> Signup and view all the answers

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