37 Questions
Which of the following is NOT a kind of staining technique?
Special
What is the purpose of fixing a slide in microbiology?
Killing the organisms
What is it called when colorless bacteria are prepared against a colored background?
Negative staining
In the Gram stain, what is the function of the mordant?
Iodine
Which dye is commonly used to stain flagella in microbiology?
Malachite green
What is the function of the diaphragm on a compound microscope?
Controls the amount of light entering the condenser
What is the function of the ocular lens in microscopy?
To remagnify the image formed by the objective lens
Which part of the microscope measures the light-bending ability of a medium?
Refractive index
In microscopy, what does the resolution refer to?
Ability to distinguish between two points
What would happen if you adjust the diaphragm to its smallest opening on a compound microscope?
The specimen will appear dimmer
What effect does changing the refractive index of a medium have on microscopy?
Affects light bending in the medium
If the ocular lens has a high magnification power, what impact does this have on the overall magnification in microscopy?
Has no effect on overall magnification
Why is it important to properly clean and maintain microscope lenses regularly?
To avoid contamination of specimens
Which microscope illuminates specimens with blue light and produces three-dimensional images?
Confocal microscope
What microscope forms an image from two sets of light rays, one from the light source and the other diffracted from a structure in the specimen?
Phase-contrast microscope
Which microscope uses a metal and diamond probe to produce a three-dimensional image?
Atomic force microscope
What type of microscopes illuminates specimens with blue light and produces three-dimensional images?
Light microscope
Which microscope utilizes a technique where only the scattered light reaches the objective lens to visualize unstained specimens?
Darkfield microscope
What microscope would be most useful in observing fine structures like cell organelles?
Transmission electron microscope
Which type of microscopy is commonly used for live-cell imaging due to its ability to provide optical sections of specimens?
Fluorescence microscopy
Microscopy that is useful for viewing transparent specimens without staining?
Phase-Contrast Microscopy
Which microscope is best suited for studying the surface structures of intact cells and viruses?
Scanning electron microscope
In transmission electron microscopy, where is the specimen typically placed?
Copper mesh grid
Which microscope is valuable for examining different layers of specimens and commonly involves shadow casting?
Transmission electron microscope
What is the primary application of a phase-contrast microscope?
Enhancing contrast in transparent samples
Which microscope is most suitable for detailed examination of the three-dimensional surface characteristics of a specimen?
Scanning electron microscope
What distinguishes a darkfield microscope from other types when observing specimens?
It illuminates the specimen against a dark background
How does a scanning electron microscope differ from a transmission electron microscope?
It uses electrons to scan specimens on the surface vs. through specimens
What advantage does a phase-contrast microscope offer when examining transparent samples?
Allows imaging without staining techniques
How are flagella stained?
Carbolfuchsin
What stain is used to visualize capsules?
Schaeffer-Fulton stain
Which staining method is employed to observe endospores?
Schaeffer-Fulton stain
At the completion of the Gram staining process, what color are gram-negative bacteria?
Pink
How does Mycobacterium leprae appear after staining with an acid-fast stain?
Red
What color are gram-negative bacteria at the completion of the Gram staining process?
Orange
Which type of staining is NOT used to visualize capsules?
Endospore stain
What color are gram-positive bacteria at the end of Gram staining?
Purple
Test your knowledge on the function of the diaphragm on a compound microscope with this PowerPoint Clicker Questions quiz based on Bradley W. Christian's materials from McLennan Community College. Explore concepts from Chapter 3 of 'Observing Microorganisms Through a Microscope' by Pearson Education, Ltd.
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