Microbiology Chapter 3: Observing Microorganisms Through a Microscope Quiz
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Questions and Answers

Which of the following is NOT a kind of staining technique?

  • Selective
  • Differential
  • Special (correct)
  • Simple

What is the purpose of fixing a slide in microbiology?

  • Staining the organisms
  • Killing the organisms (correct)
  • Illuminating the organisms
  • Magnifying the organisms

What is it called when colorless bacteria are prepared against a colored background?

  • Acid-fast staining
  • Gram staining
  • Endospore staining
  • Negative staining (correct)

In the Gram stain, what is the function of the mordant?

<p>Iodine (D)</p> Signup and view all the answers

Which dye is commonly used to stain flagella in microbiology?

<p>Malachite green (C)</p> Signup and view all the answers

What is the function of the diaphragm on a compound microscope?

<p>Controls the amount of light entering the condenser (A)</p> Signup and view all the answers

What is the function of the ocular lens in microscopy?

<p>To remagnify the image formed by the objective lens (C)</p> Signup and view all the answers

Which part of the microscope measures the light-bending ability of a medium?

<p>Refractive index (D)</p> Signup and view all the answers

In microscopy, what does the resolution refer to?

<p>Ability to distinguish between two points (B)</p> Signup and view all the answers

What would happen if you adjust the diaphragm to its smallest opening on a compound microscope?

<p>The specimen will appear dimmer (B)</p> Signup and view all the answers

What effect does changing the refractive index of a medium have on microscopy?

<p>Affects light bending in the medium (D)</p> Signup and view all the answers

If the ocular lens has a high magnification power, what impact does this have on the overall magnification in microscopy?

<p>Has no effect on overall magnification (C)</p> Signup and view all the answers

Why is it important to properly clean and maintain microscope lenses regularly?

<p>To avoid contamination of specimens (D)</p> Signup and view all the answers

Which microscope illuminates specimens with blue light and produces three-dimensional images?

<p>Confocal microscope (C)</p> Signup and view all the answers

What microscope forms an image from two sets of light rays, one from the light source and the other diffracted from a structure in the specimen?

<p>Phase-contrast microscope (A)</p> Signup and view all the answers

Which microscope uses a metal and diamond probe to produce a three-dimensional image?

<p>Atomic force microscope (C)</p> Signup and view all the answers

What type of microscopes illuminates specimens with blue light and produces three-dimensional images?

<p>Light microscope (C)</p> Signup and view all the answers

Which microscope utilizes a technique where only the scattered light reaches the objective lens to visualize unstained specimens?

<p>Darkfield microscope (B)</p> Signup and view all the answers

What microscope would be most useful in observing fine structures like cell organelles?

<p>Transmission electron microscope (A)</p> Signup and view all the answers

Which type of microscopy is commonly used for live-cell imaging due to its ability to provide optical sections of specimens?

<p>Fluorescence microscopy (B)</p> Signup and view all the answers

Microscopy that is useful for viewing transparent specimens without staining?

<p>Phase-Contrast Microscopy (C)</p> Signup and view all the answers

Which microscope is best suited for studying the surface structures of intact cells and viruses?

<p>Scanning electron microscope (B)</p> Signup and view all the answers

In transmission electron microscopy, where is the specimen typically placed?

<p>Copper mesh grid (A)</p> Signup and view all the answers

Which microscope is valuable for examining different layers of specimens and commonly involves shadow casting?

<p>Transmission electron microscope (C)</p> Signup and view all the answers

What is the primary application of a phase-contrast microscope?

<p>Enhancing contrast in transparent samples (D)</p> Signup and view all the answers

Which microscope is most suitable for detailed examination of the three-dimensional surface characteristics of a specimen?

<p>Scanning electron microscope (B)</p> Signup and view all the answers

What distinguishes a darkfield microscope from other types when observing specimens?

<p>It illuminates the specimen against a dark background (A)</p> Signup and view all the answers

How does a scanning electron microscope differ from a transmission electron microscope?

<p>It uses electrons to scan specimens on the surface vs. through specimens (A)</p> Signup and view all the answers

What advantage does a phase-contrast microscope offer when examining transparent samples?

<p>Allows imaging without staining techniques (A)</p> Signup and view all the answers

How are flagella stained?

<p>Carbolfuchsin (D)</p> Signup and view all the answers

What stain is used to visualize capsules?

<p>Schaeffer-Fulton stain (D)</p> Signup and view all the answers

Which staining method is employed to observe endospores?

<p>Schaeffer-Fulton stain (B)</p> Signup and view all the answers

At the completion of the Gram staining process, what color are gram-negative bacteria?

<p>Pink (A)</p> Signup and view all the answers

How does Mycobacterium leprae appear after staining with an acid-fast stain?

<p>Red (D)</p> Signup and view all the answers

What color are gram-negative bacteria at the completion of the Gram staining process?

<p>Orange (B)</p> Signup and view all the answers

Which type of staining is NOT used to visualize capsules?

<p>Endospore stain (C)</p> Signup and view all the answers

What color are gram-positive bacteria at the end of Gram staining?

<p>Purple (A)</p> Signup and view all the answers

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