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Questions and Answers
Which of the following describes the concept of resolution in a microscope?
Which of the following describes the concept of resolution in a microscope?
- The distance between the lens and the specimen.
- The ability to enlarge an image of a specimen.
- The maximum size at which an image can be viewed.
- The ability to see fine details and distinguish between two separate points. (correct)
What is a significant limitation of electron microscopes?
What is a significant limitation of electron microscopes?
- They cannot visualize extremely small details within a cell.
- They require extensive training to operate. (correct)
- They do not provide high magnification images.
- They can view complete cells without limitations.
When using a microscope, what is the role of the coarse focus knob?
When using a microscope, what is the role of the coarse focus knob?
- To adjust the light intensity.
- To adjust the position of the eyepiece.
- To quickly bring the specimen into general focus. (correct)
- To change the magnification power of the lens.
Which type of microscope is best suited for viewing small details within a cell at a very high magnification?
Which type of microscope is best suited for viewing small details within a cell at a very high magnification?
What is the first step when preparing to use a microscope?
What is the first step when preparing to use a microscope?
Flashcards
Magnification
Magnification
The ability to make an image appear larger.
Resolution
Resolution
The ability to distinguish between two closely spaced objects.
Light Microscope
Light Microscope
A type of microscope that uses light to illuminate the specimen.
Electron Microscope
Electron Microscope
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CARE of microscope
CARE of microscope
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Study Notes
Microscopy
- Microscopes are devices with magnifying lenses to view details and enlarged images of small objects.
- Two main functions:
- Magnification: enlarging an image, like zooming in on a specimen.
- Resolution: clarity or the ability to distinguish fine detail, to see two points as separate.
- Magnification and resolution are related; increasing magnification usually improves resolution.
- Types of microscopes:
- Light Microscopes
- Electron Microscopes
- Dissecting Microscopes
Electron Microscopes
- State-of-the-art microscopes.
- Able to achieve extremely high magnifications (up to 1,000,000x).
- Useful for viewing minute details within cells (though it can not show an entire cell).
Parts of a Light Microscope
- Eyepiece (Ocular Lens): The lens you look through.
- Body Tube: Connects the eyepiece to the objective lenses.
- Revolving Nosepiece: Holds the objective lenses.
- Objective Lenses: Magnify the image (short, medium, and long focal lengths).
- Stage Clips: Holds the slide in place.
- Stage: Platform where the slide rests.
- Diaphragm: Adjusts the amount of light passing through the specimen.
- Light Source: Provides illumination.
- Condenser: Concentrates light on the specimen.
- Coarse Adjustment Knob: Used for initial focusing.
- Fine Adjustment Knob: For precise focusing.
- Arm: Supports the body tube and lenses.
- Base: The bottom support of the microscope.
How to Use a Microscope
- Place the slide on the stage and secure it with stage clips.
- Rotate the nosepiece to select the lowest power objective lens.
- Look through the eyepiece.
- Use the coarse adjustment knob to bring the specimen into approximate focus.
- Use the fine adjustment knob for precise focus.
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