Microscopy of Babesia spp.

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18 Questions

What is the purpose of using 2% formaldehyde in the centrifugation method?

To preserve the morphology of microfilariae

What is the recommended method for preparing blood smears from capillary blood?

Thick and thin smears

What is the recommended method to examine the filter after adding one drop of 0.1% methylene blue?

Examine at 10×

What is the characteristic shape of Babesia bigemina in a blood smear?

Pear-shaped bodies

What is the purpose of preparing a thick smear in blood parasite detection?

To detect the presence of parasites with high sensitivity

What is the recommended pore size of the filter in the filtration method?

5-μm

What is the primary reason for using a cover-slip to spread the blood as a monolayer of cells in a wet blood film?

To observe the motility of Trypanosoma spp

What is the characteristic feature of Theileria equi in a blood smear?

Maltese cross formation

What is the typical size range of Babesia caballi in a blood smear?

2.5–4 μm

What is the clinical sign of Anaplasma spp. infection?

Mass destruction of RBCs

Why is 2% formalin recommended as the lysing solution in the filtration method?

It causes less changes in the size of microfilariae

What is the characteristic feature of Anaplasma spp. in a blood smear?

Dark staining body on the margin or center of RBCs

What is the recommended procedure for preparing blood smears when using venous blood?

Preparing blood smears as soon as possible after collection

What is the purpose of dipping the smears in absolute methanol before Giemsa staining?

To enhance staining of microfilariae

What is the recommended centrifugation speed and time for the centrifugation method?

500 × g for 10 minutes

What is the recommended technique for collecting blood for microfilaria detection?

Haematocrit centrifugation technique

What is the result of delaying the preparation of blood smears?

Changes in parasite morphology and staining characteristics

What is the purpose of preparing a thin smear after a thick smear has tested positive for malaria parasites?

To identify the species of malaria parasites

Identify and examine Babesia bovis and Babesia bigemina under a microscope. Learn to recognize their characteristic shapes and sizes in red blood cells.

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