Microscopy of Babesia spp.

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Questions and Answers

What is the purpose of using 2% formaldehyde in the centrifugation method?

  • To preserve the morphology of microfilariae (correct)
  • To prevent hemolysis
  • To enhance staining of microfilariae
  • To lyse red blood cells

What is the recommended method for preparing blood smears from capillary blood?

  • Only thin smears
  • Thick and thin smears (correct)
  • No smears, only centrifugation
  • Only thick smears

What is the recommended method to examine the filter after adding one drop of 0.1% methylene blue?

  • Examine at 10× (correct)
  • Examine at 100×
  • Examine under a fluorescent microscope
  • Examine at 40×

What is the characteristic shape of Babesia bigemina in a blood smear?

<p>Pear-shaped bodies (A)</p> Signup and view all the answers

What is the purpose of preparing a thick smear in blood parasite detection?

<p>To detect the presence of parasites with high sensitivity (D)</p> Signup and view all the answers

What is the recommended pore size of the filter in the filtration method?

<p>5-μm (D)</p> Signup and view all the answers

What is the primary reason for using a cover-slip to spread the blood as a monolayer of cells in a wet blood film?

<p>To observe the motility of Trypanosoma spp (A)</p> Signup and view all the answers

What is the characteristic feature of Theileria equi in a blood smear?

<p>Maltese cross formation (A)</p> Signup and view all the answers

What is the typical size range of Babesia caballi in a blood smear?

<p>2.5–4 μm (B)</p> Signup and view all the answers

What is the clinical sign of Anaplasma spp. infection?

<p>Mass destruction of RBCs (A)</p> Signup and view all the answers

Why is 2% formalin recommended as the lysing solution in the filtration method?

<p>It causes less changes in the size of microfilariae (B)</p> Signup and view all the answers

What is the characteristic feature of Anaplasma spp. in a blood smear?

<p>Dark staining body on the margin or center of RBCs (A)</p> Signup and view all the answers

What is the recommended procedure for preparing blood smears when using venous blood?

<p>Preparing blood smears as soon as possible after collection (C)</p> Signup and view all the answers

What is the purpose of dipping the smears in absolute methanol before Giemsa staining?

<p>To enhance staining of microfilariae (C)</p> Signup and view all the answers

What is the recommended centrifugation speed and time for the centrifugation method?

<p>500 × g for 10 minutes (A)</p> Signup and view all the answers

What is the recommended technique for collecting blood for microfilaria detection?

<p>Haematocrit centrifugation technique (C)</p> Signup and view all the answers

What is the result of delaying the preparation of blood smears?

<p>Changes in parasite morphology and staining characteristics (D)</p> Signup and view all the answers

What is the purpose of preparing a thin smear after a thick smear has tested positive for malaria parasites?

<p>To identify the species of malaria parasites (B)</p> Signup and view all the answers

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