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Microscopy of Babesia spp.

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Questions and Answers

What is the purpose of using 2% formaldehyde in the centrifugation method?

To preserve the morphology of microfilariae (correct)

To prevent hemolysis

To enhance staining of microfilariae

To lyse red blood cells

What is the recommended method for preparing blood smears from capillary blood?

Only thin smears

Thick and thin smears (correct)

No smears, only centrifugation

Only thick smears

What is the recommended method to examine the filter after adding one drop of 0.1% methylene blue?

Examine at 10× (correct)

Examine at 100×

Examine under a fluorescent microscope

Examine at 40×

What is the characteristic shape of Babesia bigemina in a blood smear?

Pear-shaped bodies Signup and view all the answers

What is the purpose of preparing a thick smear in blood parasite detection?

To detect the presence of parasites with high sensitivity Signup and view all the answers

What is the recommended pore size of the filter in the filtration method?

5-μm Signup and view all the answers

What is the primary reason for using a cover-slip to spread the blood as a monolayer of cells in a wet blood film?

To observe the motility of Trypanosoma spp Signup and view all the answers

What is the characteristic feature of Theileria equi in a blood smear?

Maltese cross formation Signup and view all the answers

What is the typical size range of Babesia caballi in a blood smear?

2.5–4 μm Signup and view all the answers

What is the clinical sign of Anaplasma spp. infection?

Mass destruction of RBCs Signup and view all the answers

Why is 2% formalin recommended as the lysing solution in the filtration method?

It causes less changes in the size of microfilariae Signup and view all the answers

What is the characteristic feature of Anaplasma spp. in a blood smear?

Dark staining body on the margin or center of RBCs Signup and view all the answers

What is the recommended procedure for preparing blood smears when using venous blood?

Preparing blood smears as soon as possible after collection Signup and view all the answers

What is the purpose of dipping the smears in absolute methanol before Giemsa staining?

To enhance staining of microfilariae Signup and view all the answers

What is the recommended centrifugation speed and time for the centrifugation method?

500 × g for 10 minutes Signup and view all the answers

What is the recommended technique for collecting blood for microfilaria detection?

Haematocrit centrifugation technique Signup and view all the answers

What is the result of delaying the preparation of blood smears?

Changes in parasite morphology and staining characteristics Signup and view all the answers

What is the purpose of preparing a thin smear after a thick smear has tested positive for malaria parasites?

To identify the species of malaria parasites Signup and view all the answers