Podcast
Questions and Answers
What is the first step in preparing the microscope?
What is the first step in preparing the microscope?
What should you avoid doing when switching to the 40x objective?
What should you avoid doing when switching to the 40x objective?
What is the purpose of the microscrew?
What is the purpose of the microscrew?
What should you do with the used objectives after finishing the analysis?
What should you do with the used objectives after finishing the analysis?
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What is the final step in the post-analytical phase?
What is the final step in the post-analytical phase?
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What type of preparations can be viewed with a microscope?
What type of preparations can be viewed with a microscope?
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What is the purpose of the crosses table?
What is the purpose of the crosses table?
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What should you do with the preparation after finishing the analysis?
What should you do with the preparation after finishing the analysis?
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What is the main topic of Week 1?
What is the main topic of Week 1?
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What is the purpose of the repetition in Week 3?
What is the purpose of the repetition in Week 3?
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What is the importance of following the teacher's instructions?
What is the importance of following the teacher's instructions?
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What is the goal of the course?
What is the goal of the course?
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What is the topic of the activity in Week 2?
What is the topic of the activity in Week 2?
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When is the national holiday Eid al-Adha celebrated?
When is the national holiday Eid al-Adha celebrated?
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What is the focus of the practical repetition in Week 5?
What is the focus of the practical repetition in Week 5?
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What is the final goal of the course?
What is the final goal of the course?
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What is the purpose of using a 70% alcohol solution to clean the slide?
What is the purpose of using a 70% alcohol solution to clean the slide?
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What is the function of the outer layer of the onion?
What is the function of the outer layer of the onion?
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What is the purpose of adding a drop of iodine solution to the slide?
What is the purpose of adding a drop of iodine solution to the slide?
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What is the main component of the outer layer of the human cheek?
What is the main component of the outer layer of the human cheek?
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What is the purpose of tapping the slide gently with the back of a pen or a pair of forceps?
What is the purpose of tapping the slide gently with the back of a pen or a pair of forceps?
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What is the name of the outer layer of the human cheek?
What is the name of the outer layer of the human cheek?
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What is the purpose of using a microscope to observe the sample?
What is the purpose of using a microscope to observe the sample?
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What is the function of the epithelial cells in the human cheek?
What is the function of the epithelial cells in the human cheek?
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What is the purpose of adding a drop of iodine solution to the sample?
What is the purpose of adding a drop of iodine solution to the sample?
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What type of paper is used to clean the slide?
What type of paper is used to clean the slide?
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What is the purpose of using a cotton swab to scrape the inside of your cheek?
What is the purpose of using a cotton swab to scrape the inside of your cheek?
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What is the purpose of using a liquid when preparing a sample for microscopic observation?
What is the purpose of using a liquid when preparing a sample for microscopic observation?
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What should you do with the slide after adding the iodine solution?
What should you do with the slide after adding the iodine solution?
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What is the term for the entire preparation, including the sample and the liquid, placed between two glass slides?
What is the term for the entire preparation, including the sample and the liquid, placed between two glass slides?
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What magnification should be used to view the preparation under the microscope?
What magnification should be used to view the preparation under the microscope?
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What should you do after finishing the analysis?
What should you do after finishing the analysis?
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What is the purpose of cleaning the object glass with alcohol before preparing the sample?
What is the purpose of cleaning the object glass with alcohol before preparing the sample?
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Why is it important to avoid air bubbles when placing the cover glass on top of the sample?
Why is it important to avoid air bubbles when placing the cover glass on top of the sample?
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What is the purpose of using jodiumoplossing in the preparation of the sample?
What is the purpose of using jodiumoplossing in the preparation of the sample?
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What is the term for the larger, thicker glass slide that the sample is placed on?
What is the term for the larger, thicker glass slide that the sample is placed on?
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How should the cover glass be removed after use?
How should the cover glass be removed after use?
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What is the final step in the cleaning process after preparing the sample?
What is the final step in the cleaning process after preparing the sample?
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Study Notes
Microscopy Lab Techniques
- To prepare a microscope for use, rotate the 10x objective lens and raise the condenser by unscrewing the macroscrew while looking from the side, until the preparation almost touches the objective lens.
- Then, rotate the condenser downwards and close the diaphragm.
- Look through the oculars and slide them apart or together to obtain a complete image (not two half circles).
Focusing the Microscope
- While looking through the oculars, rotate the macroscrew towards you, and the object table will move downwards. Continue until an image appears and adjust it for clarity.
- Do not touch the macroscrew again. Instead, use the microscrew for further focusing.
Observing the Preparation
- Observe the preparation by viewing multiple fields of vision.
- Note and/or draw your findings.
Post-Analytical Phase
- Turn off the light.
- Reduce the light intensity to low or 0.
- Directly rotate the smallest 4x objective lens.
- Lower the condenser using the macroscrew.
- Remove the preparation and place it in a container with disinfectant.
- First, disinfect the used objectives with tissue soaked in 70% alcohol, then disinfect all other lenses and the microscope.
Microscope Components and Functions
- The microscope has a kruistafel (stage) that can be moved left and right, and up and down, using the macroscrew.
- The lens distances are calibrated, so that once you have focused with a smaller magnification, focusing at other magnifications only requires adjustment of the microscrew.
Making a Native Preparation
- To make a native preparation, the material must be thin and transparent, and placed between two glass slides (a slide and a coverslip) with a liquid (water or a dye) in between.
- The largest and thickest glass slide is the object glass, and the smaller, thinner glass slide is the coverslip.
- The entire preparation is called a native preparation.
Microscope Handling and Safety
- Always wash your hands well and dry them with a clean towel before handling the microscope.
- When handling the microscope, follow the teacher's instructions and take necessary safety precautions.
Course Schedule
- Week 1: Introduction to microscopy, safety guidelines, and making a native preparation.
- Week 2: Making a native preparation of an onion with iodine solution, and drawing the skin cells.
- Week 3: Repeating Week 2, and updating microscope drawings.
- Week 4: Finalizing microscope drawings.
- Week 5: Repetition and final questions.
Learning Objectives
- At the end of the lesson, students can:
- Differentiate between pre-analytical, analytical, and post-analytical phases.
- Identify the components and functions of a light microscope.
- Describe the process of making a native preparation.
- Follow the instructions for making a native preparation.
Cell Observations
- The cells of the onion skin are similar to the cells of the human cheek (wangslijmvlies).
- The outer layer of the cheek is constantly renewing itself, and the cells of this layer can be observed under the microscope.
- The differences and/or similarities between the onion skin cells and the cheek cells can be observed and noted.
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Description
Learn how to prepare and focus a microscope for optimal use in a laboratory setting. This quiz covers the steps to set up and adjust the microscope for clear observation.
