Histopathology Lab 4: Dehydration and Clearing Process
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Questions and Answers

What is the purpose of washing the specimen after fixation?

  • To Stain the specimen
  • To prevent the interaction between the remaining fixative and other materials (correct)
  • To remove the fixative from the specimen
  • To fix the specimen further

    • What is the reason for not using a high concentration of alcohol initially in the dehydration process?

  • It may cause cell transformation
  • It may cause cell shrinkage (correct)
  • It may cause cell expansion
  • It may cause cell rupture

    • Why is dehydration necessary in the histopathology lab?

  • Because melted paraffin wax is neutral
  • Because melted paraffin wax is hydrophilic
  • Because melted paraffin wax is acidic
  • Because melted paraffin wax is hydrophobic (correct)

    • What determines the solution used for washing the specimen?

    <p>The composition of the fixative</p> Signup and view all the answers

    What is the minimum time required for thorough washing in running water?

    <p>3-6 hours</p> Signup and view all the answers

    What is the volume of dehydrating agent recommended compared to the tissue size?

    <p>10 times more</p> Signup and view all the answers

    What is a disadvantage of using acetone as a dehydrating agent?

    <p>It produces shrinkage and distortion of the tissue</p> Signup and view all the answers

    What is an advantage of using dioxane as a dehydrating agent?

    <p>It causes less shrinkage of tissues</p> Signup and view all the answers

    What is the effect of prolonged treatment with xylene on tissue?

    <p>Causes brittleness and distortion</p> Signup and view all the answers

    What is the purpose of passing the tissue through a series of increasing alcohol concentrations?

    <p>To dehydrate the tissue</p> Signup and view all the answers

    What is the role of a clearing agent in the tissue preparation process?

    <p>To remove and replace the dehydrating agent</p> Signup and view all the answers

    Why is chloroform less damaging to tissue than xylene?

    <p>It is slower in action</p> Signup and view all the answers

    What is the advantage of using chloroform over xylene?

    <p>Tissue can be left in it overnight</p> Signup and view all the answers

    Which of the following is a characteristic of absolute alcohol as a dehydrating agent?

    <p>It is flammable</p> Signup and view all the answers

    What is the purpose of the second 100% ethanol solution in the dehydration process?

    <p>To ensure that all water is removed from the tissue</p> Signup and view all the answers

    How long does it take to clean a small piece of tissue (less than 4mm) using xylene?

    <p>1-1.5 hours</p> Signup and view all the answers

    What is the recommended volume of dehydrating agent relative to tissue size?

    <p>10 times more than tissue size</p> Signup and view all the answers

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