17 Questions
What is the purpose of washing the specimen after fixation?
To prevent the interaction between the remaining fixative and other materials
What is the reason for not using a high concentration of alcohol initially in the dehydration process?
It may cause cell shrinkage
Why is dehydration necessary in the histopathology lab?
Because melted paraffin wax is hydrophobic
What determines the solution used for washing the specimen?
The composition of the fixative
What is the minimum time required for thorough washing in running water?
3-6 hours
What is the volume of dehydrating agent recommended compared to the tissue size?
10 times more
What is a disadvantage of using acetone as a dehydrating agent?
It produces shrinkage and distortion of the tissue
What is an advantage of using dioxane as a dehydrating agent?
It causes less shrinkage of tissues
What is the effect of prolonged treatment with xylene on tissue?
Causes brittleness and distortion
What is the purpose of passing the tissue through a series of increasing alcohol concentrations?
To dehydrate the tissue
What is the role of a clearing agent in the tissue preparation process?
To remove and replace the dehydrating agent
Why is chloroform less damaging to tissue than xylene?
It is slower in action
What is the advantage of using chloroform over xylene?
Tissue can be left in it overnight
Which of the following is a characteristic of absolute alcohol as a dehydrating agent?
It is flammable
What is the purpose of the second 100% ethanol solution in the dehydration process?
To ensure that all water is removed from the tissue
How long does it take to clean a small piece of tissue (less than 4mm) using xylene?
1-1.5 hours
What is the recommended volume of dehydrating agent relative to tissue size?
10 times more than tissue size
This quiz covers the process of washing specimens after fixation in a histopathology lab, including the purpose and methods of washing. Topics include avoiding over fixation, preventing interactions with other materials, and the types of solutions used depending on the fixative composition.
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