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Histopathology Lab 4: Dehydration and Clearing Process

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Histopathology Lab 4: Dehydration and Clearing Process

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Questions and Answers

What is the purpose of washing the specimen after fixation?

To Stain the specimen

To prevent the interaction between the remaining fixative and other materials (correct)

To remove the fixative from the specimen

To fix the specimen further

What is the reason for not using a high concentration of alcohol initially in the dehydration process?

It may cause cell transformation

It may cause cell shrinkage (correct)

It may cause cell expansion

It may cause cell rupture

Why is dehydration necessary in the histopathology lab?

Because melted paraffin wax is neutral

Because melted paraffin wax is hydrophilic

Because melted paraffin wax is acidic

Because melted paraffin wax is hydrophobic (correct)

What determines the solution used for washing the specimen?

The composition of the fixative Signup and view all the answers

What is the minimum time required for thorough washing in running water?

3-6 hours Signup and view all the answers

What is the volume of dehydrating agent recommended compared to the tissue size?

10 times more Signup and view all the answers

What is a disadvantage of using acetone as a dehydrating agent?

It produces shrinkage and distortion of the tissue Signup and view all the answers

What is an advantage of using dioxane as a dehydrating agent?

It causes less shrinkage of tissues Signup and view all the answers

What is the effect of prolonged treatment with xylene on tissue?

Causes brittleness and distortion Signup and view all the answers

What is the purpose of passing the tissue through a series of increasing alcohol concentrations?

To dehydrate the tissue Signup and view all the answers

What is the role of a clearing agent in the tissue preparation process?

To remove and replace the dehydrating agent Signup and view all the answers

Why is chloroform less damaging to tissue than xylene?

It is slower in action Signup and view all the answers

What is the advantage of using chloroform over xylene?

Tissue can be left in it overnight Signup and view all the answers

Which of the following is a characteristic of absolute alcohol as a dehydrating agent?

It is flammable Signup and view all the answers

What is the purpose of the second 100% ethanol solution in the dehydration process?

To ensure that all water is removed from the tissue Signup and view all the answers

How long does it take to clean a small piece of tissue (less than 4mm) using xylene?

1-1.5 hours Signup and view all the answers

What is the recommended volume of dehydrating agent relative to tissue size?

10 times more than tissue size Signup and view all the answers