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Questions and Answers
What is the purpose of washing the specimen after fixation?
What is the purpose of washing the specimen after fixation?
- To Stain the specimen
- To prevent the interaction between the remaining fixative and other materials (correct)
- To remove the fixative from the specimen
- To fix the specimen further
What is the reason for not using a high concentration of alcohol initially in the dehydration process?
What is the reason for not using a high concentration of alcohol initially in the dehydration process?
- It may cause cell transformation
- It may cause cell shrinkage (correct)
- It may cause cell expansion
- It may cause cell rupture
Why is dehydration necessary in the histopathology lab?
Why is dehydration necessary in the histopathology lab?
- Because melted paraffin wax is neutral
- Because melted paraffin wax is hydrophilic
- Because melted paraffin wax is acidic
- Because melted paraffin wax is hydrophobic (correct)
What determines the solution used for washing the specimen?
What determines the solution used for washing the specimen?
What is the minimum time required for thorough washing in running water?
What is the minimum time required for thorough washing in running water?
What is the volume of dehydrating agent recommended compared to the tissue size?
What is the volume of dehydrating agent recommended compared to the tissue size?
What is a disadvantage of using acetone as a dehydrating agent?
What is a disadvantage of using acetone as a dehydrating agent?
What is an advantage of using dioxane as a dehydrating agent?
What is an advantage of using dioxane as a dehydrating agent?
What is the effect of prolonged treatment with xylene on tissue?
What is the effect of prolonged treatment with xylene on tissue?
What is the purpose of passing the tissue through a series of increasing alcohol concentrations?
What is the purpose of passing the tissue through a series of increasing alcohol concentrations?
What is the role of a clearing agent in the tissue preparation process?
What is the role of a clearing agent in the tissue preparation process?
Why is chloroform less damaging to tissue than xylene?
Why is chloroform less damaging to tissue than xylene?
What is the advantage of using chloroform over xylene?
What is the advantage of using chloroform over xylene?
Which of the following is a characteristic of absolute alcohol as a dehydrating agent?
Which of the following is a characteristic of absolute alcohol as a dehydrating agent?
What is the purpose of the second 100% ethanol solution in the dehydration process?
What is the purpose of the second 100% ethanol solution in the dehydration process?
How long does it take to clean a small piece of tissue (less than 4mm) using xylene?
How long does it take to clean a small piece of tissue (less than 4mm) using xylene?
What is the recommended volume of dehydrating agent relative to tissue size?
What is the recommended volume of dehydrating agent relative to tissue size?
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