Microscopy in Pathology Labs
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Questions and Answers

What is the main difference between light microscopes and electron microscopes?

  • Light microscopes use glass lenses while electron microscopes use magnetic lenses (correct)
  • Light microscopes can achieve higher magnification than electron microscopes
  • Light microscopes use visible light while electron microscopes use UV light
  • Light microscopes are smaller in size compared to electron microscopes

    • Which of the following best describes the resolution power of electron microscopes compared to light microscopes?

  • Electron microscopes and light microscopes have the same resolution power
  • Resolution power is not related to the type of microscope used
  • Electron microscopes have a lower resolution power than light microscopes
  • Electron microscopes have a higher resolution power than light microscopes (correct)

    • What is the main purpose of using fluorescent stains in fluorescence microscopy?

  • To enhance the contrast of the specimen
  • To increase the magnification of the specimen
  • To provide a source of UV or other light for the microscope
  • To bind to specific cell macromolecules and make them visible (correct)

    • Which of the following best describes the relationship between magnification and resolution in microscopy?

    <p>Magnification and resolution are independent of each other</p> Signup and view all the answers

    What is the main purpose of the condenser lens in a light microscope?

    <p>To focus the light onto the specimen</p> Signup and view all the answers

    Which type of electron microscope is used to study the ultrastructure of tissues in ultrathin sections?

    <p>Transmission Electron Microscope (TEM)</p> Signup and view all the answers

    What is the purpose of the objective lens in a light microscope?

    <p>To magnify the image of the specimen</p> Signup and view all the answers

    What is the primary function of microscopy in pathology?

    <p>To visualize structures too small for the naked eye</p> Signup and view all the answers

    Why are electron microscopes required to operate in a vacuum?

    <p>To prevent the electrons from being absorbed by air particles</p> Signup and view all the answers

    What is the resolving power of the human eye?

    <p>0.2 mm</p> Signup and view all the answers

    What is the typical size range of a eukaryotic cell?

    <p>10 µm to 50 µm</p> Signup and view all the answers

    What is the main advantage of using a fluorescence microscope compared to a light microscope?

    <p>Ability to visualize specific cellular structures</p> Signup and view all the answers

    How do electron microscopes achieve different magnification levels?

    <p>By changing the speed of the electrons in the beam</p> Signup and view all the answers

    What is the approximate thickness of a cell membrane?

    <p>7 nm</p> Signup and view all the answers

    What is the maximum magnification power of a light microscope?

    <p>1000 times</p> Signup and view all the answers

    What type of microscopy is used to visualize sample surfaces?

    <p>Scanning Electron Microscopy (SEM)</p> Signup and view all the answers

    What is the typical thickness range for tissue sections studied by Transmission Electron Microscopy (TEM)?

    <p>40-90 nm</p> Signup and view all the answers

    What is the purpose of adding compounds with heavy metal ions to the fixative or dehydrating solutions used for tissue preparation in TEM?

    <p>To improve contrast and resolution</p> Signup and view all the answers

    In TEM images, what do areas that appear bright (electron lucent) represent?

    <p>Areas of the specimen through which electrons pass</p> Signup and view all the answers

    What is the highest resolution that can be achieved with a light microscope?

    <p>0.2 µm</p> Signup and view all the answers

    In SEM, what is the purpose of covering the sample surface with a thin layer of heavy metal?

    <p>To reflect the electrons in the scanning beam</p> Signup and view all the answers

    How is the complete image obtained in Scanning Electron Microscopy (SEM)?

    <p>By scanning the electron beam across the entire sample surface</p> Signup and view all the answers

    What is the primary purpose of microscopy in pathology?

    <p>To visualize the detailed structure of cells and tissues</p> Signup and view all the answers

    What is the key limitation of light microscopes compared to electron microscopes?

    <p>Poorer resolution</p> Signup and view all the answers

    What is the primary function of the condenser lens in a light microscope?

    <p>To focus the light beam onto the sample</p> Signup and view all the answers

    What is the typical size range of a eukaryotic cell?

    <p>10-50 µm</p> Signup and view all the answers

    What is the purpose of using heavy metal stains in sample preparation for Transmission Electron Microscopy (TEM)?

    <p>To increase the contrast of the sample</p> Signup and view all the answers

    What is the main advantage of using a fluorescence microscope compared to a standard light microscope?

    <p>Ability to visualize specific molecules or structures</p> Signup and view all the answers

    In Transmission Electron Microscopy (TEM), what is the primary reason for the appearance of areas that are darker (electron dense)?

    <p>These areas represent regions where electrons are absorbed or deflected due to higher density or the presence of heavy metal ions.</p> Signup and view all the answers

    In Scanning Electron Microscopy (SEM), what is the primary function of the thin layer of heavy metal coating applied to the sample surface?

    <p>To reflect the incident electron beam for generating the image signal.</p> Signup and view all the answers

    What is the primary advantage of using Transmission Electron Microscopy (TEM) over Scanning Electron Microscopy (SEM) for studying biological samples?

    <p>TEM provides higher magnification and resolution for visualizing internal structures.</p> Signup and view all the answers

    In Transmission Electron Microscopy (TEM), what is the primary reason for using very thin tissue sections (40-90 nm)?

    <p>To minimize the scattering and absorption of electrons as they pass through the sample.</p> Signup and view all the answers

    In Scanning Electron Microscopy (SEM), what is the primary reason for operating the instrument in a vacuum environment?

    <p>To allow the electron beam to travel without interference from air molecules.</p> Signup and view all the answers

    Which of the following statements best describes the relationship between magnification and resolution in electron microscopy?

    <p>Higher magnification can improve resolution, but only up to a certain limit determined by the instrument's optics and aberrations.</p> Signup and view all the answers

    Which type of microscope is used to study the ultrastructure of tissues in ultrathin sections?

    <p>Transmission Electron Microscope (TEM)</p> Signup and view all the answers

    What is the main advantage of using a fluorescence microscope compared to a light microscope?

    <p>Ability to visualize specific cell macromolecules</p> Signup and view all the answers

    What is the primary function of microscopy in pathology?

    <p>To diagnose and identify disease conditions</p> Signup and view all the answers

    What is the typical thickness range for tissue sections studied by Transmission Electron Microscopy (TEM)?

    <p>100-500 nanometers</p> Signup and view all the answers

    Which of the following best describes the relationship between magnification and resolution in microscopy?

    <p>Magnification and resolution are independent, meaning that increasing magnification does not affect resolution.</p> Signup and view all the answers

    What is the main purpose of the condenser lens in a light microscope?

    <p>To focus the light on the sample</p> Signup and view all the answers

    How do electron microscopes achieve different magnification levels?

    <p>By modulating the speed of the electrons, which also modifies the wavelength frequency</p> Signup and view all the answers

    What is the approximate thickness of a cell membrane?

    <p>10-100 nanometers</p> Signup and view all the answers

    In SEM, what is the purpose of covering the sample surface with a thin layer of heavy metal?

    <p>To reduce the charging effect on the sample surface</p> Signup and view all the answers

    What is the highest resolution that can be achieved with a light microscope?

    <p>The resolution of a light microscope is limited by the wavelength of visible light and cannot exceed approximately 200 nanometers.</p> Signup and view all the answers

    The resolving power of the human eye is 0.02 mm.

    <p>False</p> Signup and view all the answers

    A typical eukaryote cell is commonly between 10 and 50mm in size.

    <p>False</p> Signup and view all the answers

    Cell membranes are about 7cm thick.

    <p>False</p> Signup and view all the answers

    Light microscopes use ultraviolet light to magnify samples.

    <p>False</p> Signup and view all the answers

    Light microscopes have a resolving power of 0.02 µm.

    <p>False</p> Signup and view all the answers

    Electron microscopes operate in a non-vacuum environment.

    <p>False</p> Signup and view all the answers

    Electron microscopes use glass lenses to magnify tissue samples.

    <p>False</p> Signup and view all the answers

    Fluorescence microscopy relies on the wavelength of visible light to visualize samples.

    <p>False</p> Signup and view all the answers

    Light microscopes can achieve a maximum magnification of 1500x.

    <p>True</p> Signup and view all the answers

    The resolution of an image can always be increased by further magnification.

    <p>False</p> Signup and view all the answers

    Transmission Electron Microscopes (TEM) are used mainly for observing the surfaces of samples.

    <p>False</p> Signup and view all the answers

    Scanning Electron Microscopes (SEM) operate with electrons modulated at different speeds.

    <p>False</p> Signup and view all the answers

    Fluorescent compounds used in microscopy have no specific affinity for cell macromolecules.

    <p>False</p> Signup and view all the answers

    Acridine orange is an example of a compound that specifically binds to proteins.

    <p>False</p> Signup and view all the answers

    Resolution around 3 nm can be achieved with Scanning Electron Microscopes (SEM).

    <p>False</p> Signup and view all the answers

    Light is transmitted through a condenser before passing through the sample in electron microscopes.

    <p>False</p> Signup and view all the answers

    Scanning Electron Microscopy (SEM) uses a wider beam of electrons compared to Transmission Electron Microscopy (TEM).

    <p>False</p> Signup and view all the answers

    Areas that appear bright in TEM images are denser and bind heavy metal ions during specimen preparation.

    <p>False</p> Signup and view all the answers

    In Transmission Electron Microscopy (TEM), the electron beam scans the specimen surface to capture reflected electrons for image production.

    <p>False</p> Signup and view all the answers

    Heavy metal ions are added to the fixative or dehydrating solutions in SEM to enhance contrast and resolution.

    <p>False</p> Signup and view all the answers

    Electron microscopes operate in a vacuum to prevent electron absorption by air molecules.

    <p>True</p> Signup and view all the answers

    Scanning Electron Microscopy (SEM) produces black-and-white images by capturing transmitted electrons.

    <p>False</p> Signup and view all the answers

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