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Microscopy in Microbiology

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Questions and Answers

What is the total magnification when using a 40x objective lens and a 10x ocular lens?

The total magnification is $10X \times 40X = 400X$.

What are the three principles of light microscopy?

The three principles of light microscopy are magnification, resolution, and contrast.

What are the two most widely used methods of microscopy in clinical laboratories?

The two most widely used methods of microscopy in clinical laboratories are bright-field (light microscopy) and fluorescence microscopy.

How is microscopy used in diagnostic microbiology?

<p>Microscopy is used to directly examine patient specimens as well as cultivated organisms. It is also used with specific stains or dyes to detect organisms in clinical specimens.</p> Signup and view all the answers

Why is simply enlarging an object not sufficient to see detail in light microscopy?

<p>Simply enlarging an object is not sufficient to see detail because two close together give overlapping images and may merge into one.</p> Signup and view all the answers