Microscopy in Biology

Microscopy in Biology

Microscope Maintenance

• Regular cleaning and maintenance are essential to ensure optimal performance and extend the lifespan of the microscope.
• Clean the microscope lenses with a soft, dry cloth and avoid touching the lenses with bare hands.
• Store the microscope in a dry, cool place, away from direct sunlight.
• Calibrate the microscope regularly to ensure accurate measurements.
• Perform routine checks on the microscope's mechanical components, such as the stage and focus knobs.

Cell Observation

• Microscopy allows for the observation of cell morphology, structure, and behavior.
• Cells can be observed in their natural state or stained to enhance visibility.
• Microscopy techniques can be used to study cellular processes, such as cell division, migration, and signaling.
• Microscopy can be used to identify and diagnose cellular abnormalities and diseases.

Microscope Types

Light Microscopes

• Use visible light to illuminate samples.
• Include:
  • Brightfield microscopes: use transmitted light to observe samples.
  • Fluorescence microscopes: use fluorescent dyes to observe specific structures.
  • Phase contrast microscopes: use differences in refractive index to observe samples.

Electron Microscopes

• Use a beam of electrons to produce an image.
• Include:
  • Transmission electron microscopes (TEM): use a beam of electrons to image samples.
  • Scanning electron microscopes (SEM): use a focused beam of electrons to produce a high-resolution image.

Specialized Microscopes

• Include:
  • Confocal microscopes: use lasers to produce high-resolution, three-dimensional images.
  • Super-resolution microscopes: use specialized techniques to achieve resolutions beyond the diffraction limit.
  • Atomic force microscopes: use a physical probe to image samples at the atomic level.