Microscopy in Biology

Study Notes

Microscopy in Biology

Microscope Maintenance

Regular cleaning and maintenance are essential to ensure optimal performance and extend the lifespan of the microscope.
Clean the microscope lenses with a soft, dry cloth and avoid touching the lenses with bare hands.
Store the microscope in a dry, cool place, away from direct sunlight.
Calibrate the microscope regularly to ensure accurate measurements.
Perform routine checks on the microscope's mechanical components, such as the stage and focus knobs.

Cell Observation

Microscopy allows for the observation of cell morphology, structure, and behavior.
Cells can be observed in their natural state or stained to enhance visibility.
Microscopy techniques can be used to study cellular processes, such as cell division, migration, and signaling.
Microscopy can be used to identify and diagnose cellular abnormalities and diseases.

Microscope Types

Light Microscopes

Use visible light to illuminate samples.
Include:
- Brightfield microscopes: use transmitted light to observe samples.
- Fluorescence microscopes: use fluorescent dyes to observe specific structures.
- Phase contrast microscopes: use differences in refractive index to observe samples.

Electron Microscopes

Use a beam of electrons to produce an image.
Include:
- Transmission electron microscopes (TEM): use a beam of electrons to image samples.
- Scanning electron microscopes (SEM): use a focused beam of electrons to produce a high-resolution image.

Specialized Microscopes

Include:
- Confocal microscopes: use lasers to produce high-resolution, three-dimensional images.
- Super-resolution microscopes: use specialized techniques to achieve resolutions beyond the diffraction limit.
- Atomic force microscopes: use a physical probe to image samples at the atomic level.

Description

Learn about the maintenance and types of microscopes, as well as their applications in observing cell morphology and behavior. Discover the different types of light and electron microscopes, and their uses in biology.