Cell Biology and Microscopy Quiz
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Questions and Answers

Which of the following is NOT a type of light microscopy?

  • Differential Interference Contrast
  • Transmission Electron Microscopy (correct)
  • Phase-contrast
  • Bright-field
  • Scanning Electron Microscopy (SEM) provides a 3D image of the internal structure of a sample.

    False (B)

    What is the main advantage of using electron microscopy over light microscopy?

    Electron microscopy offers much higher magnification and resolution, enabling the visualization of structures at the molecular level.

    The technique that uses visible light and lenses to magnify a sample is called ____ microscopy.

    <p>light</p> Signup and view all the answers

    Match the microscopy type with its characteristic:

    <p>Bright-field = Uses differences in contrast between the specimen and its surroundings Phase-contrast = Enhances contrast by converting differences in refractive index into variations in brightness Differential interference contrast = Creates an image with a 3D-like appearance by exaggerating subtle differences in refractive index Dark-field = Illuminates the specimen from the sides, making it appear bright against a dark background Fluorescence = Uses fluorescent dyes to illuminate specific structures in the specimen</p> Signup and view all the answers

    Fimbria are filamentous structures made of proteins that extend from the cell surface and assist with attachment.

    <p>True (A)</p> Signup and view all the answers

    Which of the following structures are NOT part of the cell envelope?

    <p>Nucleus (B)</p> Signup and view all the answers

    Which of the following is NOT a function of fimbria and pili?

    <p>Photosynthesis (D)</p> Signup and view all the answers

    The cytoplasmic membrane is found in both bacteria and eukaryotes.

    <p>True (A)</p> Signup and view all the answers

    What is the primary function of poly-β-hydroxyalkanoates (PHAs) in prokaryotes?

    <p>Carbon storage</p> Signup and view all the answers

    Prokaryotes accumulate ______ granules when phosphate is in excess and can use them as a source of phosphate for nucleic acid and phospholipid biosynthesis when phosphate is limiting.

    <p>polyphosphate</p> Signup and view all the answers

    What are the two types of membrane proteins found in the cytoplasmic membrane?

    <p>Integral and peripheral membrane proteins</p> Signup and view all the answers

    Match the following structures with their functions:

    <p>Fimbria = Attachment</p> Signup and view all the answers

    Which of the following is NOT a difference between the cytoplasmic membrane in Archaea and Bacteria/Eukarya?

    <p>Presence of a cell wall (B)</p> Signup and view all the answers

    Archaea can live in extremely ______ environments due to the unique structure of their cytoplasmic membrane.

    <p>extreme</p> Signup and view all the answers

    What is the primary function of the cell wall?

    <p>Protects against osmotic lysis and maintains cell shape (C)</p> Signup and view all the answers

    Match the following cell wall types with their corresponding bacterial groups.

    <p>Gram-positive = Thick peptidoglycan layer, teichoic acids Gram-negative = Thin peptidoglycan layer, outer membrane Mycobacteria = No cell wall, unique waxy layer</p> Signup and view all the answers

    All bacteria have a cell wall.

    <p>False (B)</p> Signup and view all the answers

    What happens to sulfur granules when they oxidize to sulfate (SO42-)?

    <p>They slowly disappear (B)</p> Signup and view all the answers

    Gas vesicles are structures that help bacterial cells to sink in water.

    <p>False (B)</p> Signup and view all the answers

    What is the primary function of bacterial flagella?

    <p>To push or pull the cell through liquid.</p> Signup and view all the answers

    Gloeomargarita is a cyanobacterium containing granules of the mineral ________.

    <p>benstonite</p> Signup and view all the answers

    Match the following organisms with their corresponding structures or characteristics:

    <p>Heliobacterium modesticaldum = Contains sulfur granules Gloeomargarita = Contains benstonite granules Bacterial flagella = Cell locomotion Gas vesicles = Buoyancy in water</p> Signup and view all the answers

    What type of surface motility involves the smooth motion along the long axis of a cell without external propulsive structures?

    <p>Gliding (D)</p> Signup and view all the answers

    Chemotaxis is the movement of organisms towards or away from light.

    <p>False (B)</p> Signup and view all the answers

    What is required for gliding motility in bacteria?

    <p>Adhesion proteins</p> Signup and view all the answers

    Type IV pili are responsible for ______ motility.

    <p>twitching</p> Signup and view all the answers

    Match the type of taxis with its corresponding characteristic:

    <p>Chemotaxis = Movement towards or away from a chemical gradient Phototaxis = Movement towards or away from light Magnetotaxis = Movement in response to magnetic fields Thigmotaxis = Movement in response to touch or physical contact</p> Signup and view all the answers

    What is the primary function of porins in the outer membrane?

    <p>Serve as channels for solute transport (A)</p> Signup and view all the answers

    The periplasm is located outside the cytoplasmic membrane.

    <p>False (B)</p> Signup and view all the answers

    What role do S-layers play in bacteria and archaea?

    <p>They provide structural strength and protect the cell from osmotic lysis.</p> Signup and view all the answers

    Capsules and slime layers are involved in attachment and __________.

    <p>protection</p> Signup and view all the answers

    Which of the following is NOT a component found in the cell surface inclusions?

    <p>Hydrolytic enzymes (C)</p> Signup and view all the answers

    The outer membrane is less permeable than the cytoplasmic membrane.

    <p>False (B)</p> Signup and view all the answers

    What structural feature do capsules provide for bacteria?

    <p>A sticky coat of polysaccharides</p> Signup and view all the answers

    Flashcards

    Light microscopy

    A technique using visible light and lenses to magnify samples.

    Gram staining

    A method to classify bacteria based on their cell wall composition using specific dyes.

    Electron microscopy

    A microscopy technique that uses electrons for higher magnification and resolution than light microscopy.

    Transmission Electron Microscopy (TEM)

    A type of electron microscopy that transmits electrons through a thin sample to observe internal structures.

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    Scanning Electron Microscopy (SEM)

    A type of electron microscopy that scans a sample's surface with electrons to create 3D images.

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    Cell envelope

    The outer structure of prokaryotic cells, including the cytoplasmic membrane, cell wall, and outer membrane.

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    Cytoplasmic membrane

    The innermost layer of the cell envelope, controlling what enters and exits the cell.

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    Cell locomotion

    The ability of cells to move, often through structures like flagella or cilia.

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    Phosphate (P)

    A crucial nutrient and component for nucleic acids and ATP in cells.

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    Porins

    Proteins unique to the outer membrane that act as channels for substance transport.

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    Periplasm

    The space between the outer membrane and the cytoplasmic membrane in bacteria.

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    S-layers

    Outer layers found in bacteria and archaea, providing structural strength and protection.

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    Capsules and Slime Layers

    Sticky coats of polysaccharides outside the cell for attachment and protection.

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    Fimbriae and Pili

    Hair-like structures on bacteria used for attachment to surfaces.

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    Hydrolytic Enzymes

    Enzymes located in the periplasm involved in the degradation of substances.

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    Gas Vesicles

    Structures in certain bacteria that provide buoyancy in water.

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    Fimbria

    Filamentous structures on the cell surface that aid attachment.

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    Pili

    Thin, hair-like structures that facilitate genetic exchange and motility.

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    Poly-β-hydroxyalkanoates

    Carbon storage polymers found in prokaryotes, stored as granules.

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    Glycogen

    A common carbon storage molecule used by cells when needed.

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    Polyphosphate granules

    Storage form of inorganic phosphate used during nutrient limitation.

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    Bacteria vs Archaea membranes

    Bacteria have ester-linked fatty acids; Archaea have ether-linked isoprenoid chains.

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    Major functions of cytoplasmic membrane

    Regulates entry/exit of substances, maintains gradient.

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    Cell wall

    A layer outside the cytoplasmic membrane that protects against osmotic lysis.

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    Gram-positive vs Gram-negative

    Gram-positive have thick cell walls; Gram-negative have thinner walls with outer membranes.

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    Extreme environments and Archaea

    Archaea can thrive in high-pressure or extreme temperatures, unlike most cells.

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    Mycobacteria unique feature

    Mycobacteria do not have a traditional cell wall.

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    Gliding motility

    Smooth motion along a cell's long axis without external structures.

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    Adhesive proteins

    Proteins that mediate cell adhesion during gliding motility.

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    Surface twitching

    Movement caused by the extension and retraction of type IV pili.

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    Chemotaxis

    Directed movement of organisms toward or away from a chemical gradient.

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    Phototaxis

    Movement of organisms toward or away from light.

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    Heliobacterium modesticaldum

    A species of bacteria known for its unique metabolic processes and ability to oxidize sulfur.

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    Bacterial flagella

    Long, whip-like structures that enable bacterial movement by rotating.

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    Archaeal archaella

    Similar to flagella, but found in archaea and also help in movement through rotation.

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    Gloeomargarita

    A cyanobacterium that contains granules of the mineral benstonite.

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    Study Notes

    Prokaryotic Cell Structure - BIO-440

    • The course covers prokaryotic cell structure, focusing on various components and their functions.
    • Topics include: introduction to microscopy and cell structure, the cell envelope (cytoplasmic membrane, cell wall, outer membrane, S-layers), cell surface and inclusions, cell locomotion, and chemotaxis.
    • Early microscopy: Robert Hooke used a microscope in 1664 and Antoni van Leeuwenhoek created drawings of bacteria in 1684.
    • Light microscopy: uses visible light and lenses to magnify specimens. Contrast is crucial for visualization using methods like bright-field, phase-contrast, differential interference contrast, dark-field, and fluorescence. Samples may require staining for contrast. Techniques of Gram staining and fluorescent stains are mentioned.
    • Electron Microscopy: uses electrons instead of light. High magnification and resolution, allowing viewing of structures at the molecular level. Transmission electron microscopy (TEM) sends electrons through a thin sample to study internal structures, while scanning electron microscopy (SEM) scans the surface to create a 3D image.

    Cell Envelope

    • The cell envelope is a layered structure surrounding the cytoplasm, governing interactions with the external environment.
    • Components include: cytoplasmic membrane, cell wall, outer membrane, and S-layers.

    Cytoplasmic Membrane

    • Surrounds the cytoplasm.
    • Composed of a phospholipid bilayer.
    • Contains hydrophilic head groups and hydrophobic tails (present in bacteria and eukarya).
    • Integral proteins (embedded in membrane) and peripheral proteins (associated with membrane surface) are present. Essential for interactions with environments.
    • Functions: permeability barrier—controls entry/exit of substances, protein anchor—site for proteins involved in transport, bioenergetics, and chemotaxis, and energy conservation—site of generation and dissipation of proton motive force.
    • Bacteria and Archaea have variations in membrane structure, impacting survival in extreme conditions. Archaea use ether linkages in their membranes

    Cell Wall

    • Layer outside the cytoplasmic membrane.
    • Provides protection against osmotic lysis (bursting due to water pressure).
    • Maintains cell shape and rigidity.
    • Gram-positive cell walls are thick, while gram-negative cell walls are thinner. Structure differs depending on whether bacteria is gram positive or gram negative.
    • Peptidoglycan is the major component of bacterial cell walls (polysaccharide backbone).
    • Archaea have a different type of cell wall without peptidoglycan called pseudomurein.
    • Mycobacteria do not have a cell wall.

    Outer Membrane

    • Second lipid bilayer exterior to the cell wall (only in Gram-negative bacteria).
    • Structure and functions distinct from cytoplasmic membrane.
    • Contains lipopolysaccharide (LPS).
    • Porins are channels that create permeability. Outer membrane is more permeable than cytoplasmic membrane.
    • Functions for surface recognition, virulence factor, mechanical strength.

    LPS (Lipopolysaccharide)

    • Often referred to as endotoxin due to toxic effects.
    • Highly variable among species.
    • Components include: O-specific polysaccharide, core polysaccharide, and lipid A.

    Porins

    • Unique to outer membrane.
    • Act as channels for the passage of solutes.

    Periplasm

    • Region between the outer and cytoplasmic membranes.
    • Contains hydrolytic enzymes, binding proteins (for transport), and chemoreceptors.

    S-layers

    • Found in many bacteria and nearly all archaea.
    • Always the outermost layer of the cell envelope.
    • May play the role of the cell wall, contributing to structural strength, protection against osmotic lysis, and maintaining cell shape.
    • May form a periplasmic-like space in Archaea.
    • Different configurations of the cell envelope structures are shown across various species.

    Cell surface and inclusions

    • Includes: capsules and slime layers, fimbriae and pili, carbon storage polymers, polyphosphate, sulfur, and carbonate minerals, and gas vesicles.

    Capsules and slime layers

    • Sticky polysaccharide layers exterior to the cell envelope.
    • Involved in attachment and protection.

    Fimbriae and pili

    • Filamentous protein structures extending from the cell surface.
    • Functions in attachment, motility, cell recognition, and genetic exchange.

    Carbon storage polymers

    • Poly-β-hydroxyalkanoates (PHAs) and glycogen are common intracellular carbon storage inclusions.

    Polyphosphate, sulfur, and carbonate minerals

    • Inorganic materials that microbes accumulate as storage.

    Gas vesicles

    • Confer buoyancy to some bacteria and archaea allowing the positioning in the water column where it's best for them metabolically.

    Cell Locomotion

    • Flagella (bacteria) and archaella (archaea): tiny rotating machines that push or pull the cell through liquids.
    • Polar flagella: attached to one end or both ends of the cell.
    • Peritrichous flagella: groups or tufts of flagella around the cell, causing rotation.

    Flagella structure and functions

    • Flagellar motor: composed of several proteins anchored in the cytoplasmic membrane, and cell wall.
    • Components include the filament, hook, rings.
    • Proton motive force drives the rotation of the flagellum for movement.

    Surface motility

    • Differs from flagella-driven motility
    • Additional ways bacteria move
    • Gliding: smooth motion along the long axis of a cell without external structures like pili/attachment organelles
    • Twitching: driven by the extension/retraction of type IV pili
    • Additional configurations seen in different prokaryotic organisms

    Chemotaxis

    • Directed movement toward (positive) or away from (negative) a chemical gradient.
    • A method for measuring chemotaxis utilizes a chemotaxis assay.

    Other taxis (Phototaxis and Magnetotaxis)

    • Phototaxis: movement in response to light.
    • Magnetotaxis: movement in response to magnetic fields. Different configurations observed in different species.

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    Test your knowledge on microscopy types and cellular structures in this quiz focused on cell biology. Covering topics from light microscopy to the functions of fimbria and membrane proteins, this quiz challenges your understanding of essential biological concepts.

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