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Questions and Answers
Which of the following is NOT a type of light microscopy?
Which of the following is NOT a type of light microscopy?
Scanning Electron Microscopy (SEM) provides a 3D image of the internal structure of a sample.
Scanning Electron Microscopy (SEM) provides a 3D image of the internal structure of a sample.
False (B)
What is the main advantage of using electron microscopy over light microscopy?
What is the main advantage of using electron microscopy over light microscopy?
Electron microscopy offers much higher magnification and resolution, enabling the visualization of structures at the molecular level.
The technique that uses visible light and lenses to magnify a sample is called ____ microscopy.
The technique that uses visible light and lenses to magnify a sample is called ____ microscopy.
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Match the microscopy type with its characteristic:
Match the microscopy type with its characteristic:
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Fimbria are filamentous structures made of proteins that extend from the cell surface and assist with attachment.
Fimbria are filamentous structures made of proteins that extend from the cell surface and assist with attachment.
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Which of the following structures are NOT part of the cell envelope?
Which of the following structures are NOT part of the cell envelope?
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Which of the following is NOT a function of fimbria and pili?
Which of the following is NOT a function of fimbria and pili?
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The cytoplasmic membrane is found in both bacteria and eukaryotes.
The cytoplasmic membrane is found in both bacteria and eukaryotes.
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What is the primary function of poly-β-hydroxyalkanoates (PHAs) in prokaryotes?
What is the primary function of poly-β-hydroxyalkanoates (PHAs) in prokaryotes?
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Prokaryotes accumulate ______ granules when phosphate is in excess and can use them as a source of phosphate for nucleic acid and phospholipid biosynthesis when phosphate is limiting.
Prokaryotes accumulate ______ granules when phosphate is in excess and can use them as a source of phosphate for nucleic acid and phospholipid biosynthesis when phosphate is limiting.
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What are the two types of membrane proteins found in the cytoplasmic membrane?
What are the two types of membrane proteins found in the cytoplasmic membrane?
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Match the following structures with their functions:
Match the following structures with their functions:
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Which of the following is NOT a difference between the cytoplasmic membrane in Archaea and Bacteria/Eukarya?
Which of the following is NOT a difference between the cytoplasmic membrane in Archaea and Bacteria/Eukarya?
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Archaea can live in extremely ______ environments due to the unique structure of their cytoplasmic membrane.
Archaea can live in extremely ______ environments due to the unique structure of their cytoplasmic membrane.
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What is the primary function of the cell wall?
What is the primary function of the cell wall?
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Match the following cell wall types with their corresponding bacterial groups.
Match the following cell wall types with their corresponding bacterial groups.
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All bacteria have a cell wall.
All bacteria have a cell wall.
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What happens to sulfur granules when they oxidize to sulfate (SO42-)?
What happens to sulfur granules when they oxidize to sulfate (SO42-)?
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Gas vesicles are structures that help bacterial cells to sink in water.
Gas vesicles are structures that help bacterial cells to sink in water.
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What is the primary function of bacterial flagella?
What is the primary function of bacterial flagella?
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Gloeomargarita is a cyanobacterium containing granules of the mineral ________.
Gloeomargarita is a cyanobacterium containing granules of the mineral ________.
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Match the following organisms with their corresponding structures or characteristics:
Match the following organisms with their corresponding structures or characteristics:
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What type of surface motility involves the smooth motion along the long axis of a cell without external propulsive structures?
What type of surface motility involves the smooth motion along the long axis of a cell without external propulsive structures?
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Chemotaxis is the movement of organisms towards or away from light.
Chemotaxis is the movement of organisms towards or away from light.
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What is required for gliding motility in bacteria?
What is required for gliding motility in bacteria?
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Type IV pili are responsible for ______ motility.
Type IV pili are responsible for ______ motility.
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Match the type of taxis with its corresponding characteristic:
Match the type of taxis with its corresponding characteristic:
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What is the primary function of porins in the outer membrane?
What is the primary function of porins in the outer membrane?
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The periplasm is located outside the cytoplasmic membrane.
The periplasm is located outside the cytoplasmic membrane.
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What role do S-layers play in bacteria and archaea?
What role do S-layers play in bacteria and archaea?
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Capsules and slime layers are involved in attachment and __________.
Capsules and slime layers are involved in attachment and __________.
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Which of the following is NOT a component found in the cell surface inclusions?
Which of the following is NOT a component found in the cell surface inclusions?
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The outer membrane is less permeable than the cytoplasmic membrane.
The outer membrane is less permeable than the cytoplasmic membrane.
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What structural feature do capsules provide for bacteria?
What structural feature do capsules provide for bacteria?
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Flashcards
Light microscopy
Light microscopy
A technique using visible light and lenses to magnify samples.
Gram staining
Gram staining
A method to classify bacteria based on their cell wall composition using specific dyes.
Electron microscopy
Electron microscopy
A microscopy technique that uses electrons for higher magnification and resolution than light microscopy.
Transmission Electron Microscopy (TEM)
Transmission Electron Microscopy (TEM)
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Scanning Electron Microscopy (SEM)
Scanning Electron Microscopy (SEM)
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Cell envelope
Cell envelope
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Cytoplasmic membrane
Cytoplasmic membrane
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Cell locomotion
Cell locomotion
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Phosphate (P)
Phosphate (P)
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Porins
Porins
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Periplasm
Periplasm
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S-layers
S-layers
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Capsules and Slime Layers
Capsules and Slime Layers
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Fimbriae and Pili
Fimbriae and Pili
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Hydrolytic Enzymes
Hydrolytic Enzymes
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Gas Vesicles
Gas Vesicles
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Fimbria
Fimbria
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Pili
Pili
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Poly-β-hydroxyalkanoates
Poly-β-hydroxyalkanoates
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Glycogen
Glycogen
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Polyphosphate granules
Polyphosphate granules
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Bacteria vs Archaea membranes
Bacteria vs Archaea membranes
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Major functions of cytoplasmic membrane
Major functions of cytoplasmic membrane
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Cell wall
Cell wall
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Gram-positive vs Gram-negative
Gram-positive vs Gram-negative
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Extreme environments and Archaea
Extreme environments and Archaea
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Mycobacteria unique feature
Mycobacteria unique feature
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Gliding motility
Gliding motility
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Adhesive proteins
Adhesive proteins
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Surface twitching
Surface twitching
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Chemotaxis
Chemotaxis
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Phototaxis
Phototaxis
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Heliobacterium modesticaldum
Heliobacterium modesticaldum
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Bacterial flagella
Bacterial flagella
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Archaeal archaella
Archaeal archaella
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Gloeomargarita
Gloeomargarita
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Study Notes
Prokaryotic Cell Structure - BIO-440
- The course covers prokaryotic cell structure, focusing on various components and their functions.
- Topics include: introduction to microscopy and cell structure, the cell envelope (cytoplasmic membrane, cell wall, outer membrane, S-layers), cell surface and inclusions, cell locomotion, and chemotaxis.
- Early microscopy: Robert Hooke used a microscope in 1664 and Antoni van Leeuwenhoek created drawings of bacteria in 1684.
- Light microscopy: uses visible light and lenses to magnify specimens. Contrast is crucial for visualization using methods like bright-field, phase-contrast, differential interference contrast, dark-field, and fluorescence. Samples may require staining for contrast. Techniques of Gram staining and fluorescent stains are mentioned.
- Electron Microscopy: uses electrons instead of light. High magnification and resolution, allowing viewing of structures at the molecular level. Transmission electron microscopy (TEM) sends electrons through a thin sample to study internal structures, while scanning electron microscopy (SEM) scans the surface to create a 3D image.
Cell Envelope
- The cell envelope is a layered structure surrounding the cytoplasm, governing interactions with the external environment.
- Components include: cytoplasmic membrane, cell wall, outer membrane, and S-layers.
Cytoplasmic Membrane
- Surrounds the cytoplasm.
- Composed of a phospholipid bilayer.
- Contains hydrophilic head groups and hydrophobic tails (present in bacteria and eukarya).
- Integral proteins (embedded in membrane) and peripheral proteins (associated with membrane surface) are present. Essential for interactions with environments.
- Functions: permeability barrier—controls entry/exit of substances, protein anchor—site for proteins involved in transport, bioenergetics, and chemotaxis, and energy conservation—site of generation and dissipation of proton motive force.
- Bacteria and Archaea have variations in membrane structure, impacting survival in extreme conditions. Archaea use ether linkages in their membranes
Cell Wall
- Layer outside the cytoplasmic membrane.
- Provides protection against osmotic lysis (bursting due to water pressure).
- Maintains cell shape and rigidity.
- Gram-positive cell walls are thick, while gram-negative cell walls are thinner. Structure differs depending on whether bacteria is gram positive or gram negative.
- Peptidoglycan is the major component of bacterial cell walls (polysaccharide backbone).
- Archaea have a different type of cell wall without peptidoglycan called pseudomurein.
- Mycobacteria do not have a cell wall.
Outer Membrane
- Second lipid bilayer exterior to the cell wall (only in Gram-negative bacteria).
- Structure and functions distinct from cytoplasmic membrane.
- Contains lipopolysaccharide (LPS).
- Porins are channels that create permeability. Outer membrane is more permeable than cytoplasmic membrane.
- Functions for surface recognition, virulence factor, mechanical strength.
LPS (Lipopolysaccharide)
- Often referred to as endotoxin due to toxic effects.
- Highly variable among species.
- Components include: O-specific polysaccharide, core polysaccharide, and lipid A.
Porins
- Unique to outer membrane.
- Act as channels for the passage of solutes.
Periplasm
- Region between the outer and cytoplasmic membranes.
- Contains hydrolytic enzymes, binding proteins (for transport), and chemoreceptors.
S-layers
- Found in many bacteria and nearly all archaea.
- Always the outermost layer of the cell envelope.
- May play the role of the cell wall, contributing to structural strength, protection against osmotic lysis, and maintaining cell shape.
- May form a periplasmic-like space in Archaea.
- Different configurations of the cell envelope structures are shown across various species.
Cell surface and inclusions
- Includes: capsules and slime layers, fimbriae and pili, carbon storage polymers, polyphosphate, sulfur, and carbonate minerals, and gas vesicles.
Capsules and slime layers
- Sticky polysaccharide layers exterior to the cell envelope.
- Involved in attachment and protection.
Fimbriae and pili
- Filamentous protein structures extending from the cell surface.
- Functions in attachment, motility, cell recognition, and genetic exchange.
Carbon storage polymers
- Poly-β-hydroxyalkanoates (PHAs) and glycogen are common intracellular carbon storage inclusions.
Polyphosphate, sulfur, and carbonate minerals
- Inorganic materials that microbes accumulate as storage.
Gas vesicles
- Confer buoyancy to some bacteria and archaea allowing the positioning in the water column where it's best for them metabolically.
Cell Locomotion
- Flagella (bacteria) and archaella (archaea): tiny rotating machines that push or pull the cell through liquids.
- Polar flagella: attached to one end or both ends of the cell.
- Peritrichous flagella: groups or tufts of flagella around the cell, causing rotation.
Flagella structure and functions
- Flagellar motor: composed of several proteins anchored in the cytoplasmic membrane, and cell wall.
- Components include the filament, hook, rings.
- Proton motive force drives the rotation of the flagellum for movement.
Surface motility
- Differs from flagella-driven motility
- Additional ways bacteria move
- Gliding: smooth motion along the long axis of a cell without external structures like pili/attachment organelles
- Twitching: driven by the extension/retraction of type IV pili
- Additional configurations seen in different prokaryotic organisms
Chemotaxis
- Directed movement toward (positive) or away from (negative) a chemical gradient.
- A method for measuring chemotaxis utilizes a chemotaxis assay.
Other taxis (Phototaxis and Magnetotaxis)
- Phototaxis: movement in response to light.
- Magnetotaxis: movement in response to magnetic fields. Different configurations observed in different species.
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Description
Test your knowledge on microscopy types and cellular structures in this quiz focused on cell biology. Covering topics from light microscopy to the functions of fimbria and membrane proteins, this quiz challenges your understanding of essential biological concepts.