Microscopic Diagnosis of Amebiasis
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Questions and Answers

What is the standard method for diagnosing Amebiasis?

  • Stool culture using Robinson’s and Inoki medium
  • Computed tomography (CT scan)
  • Ultrasound
  • Detection of antibodies using ELISA (correct)

    • Which method is used to differentiate Entamoeba spp from white blood cells?

  • Saline and Methylene Blue (correct)
  • Stool culture using Robinson’s and Inoki medium
  • PCR
  • Ultrasound

    • What is more sensitive than Direct Fecal Smear (DFS) for diagnosing Amebiasis?

  • Ultrasound
  • Computed tomography (CT scan)
  • ELISA with PCR (correct)
  • Stool culture using Robinson’s and Inoki medium

    • Which method is not mentioned as a key diagnostic tool for Amebic Liver Abscess (ALA)?

    <p>Computerized tomography (CT scan)</p> Signup and view all the answers

    Which method is specifically used to observe the nucleus and karyosome to differentiate E.histolytica from other species?

    <p>Saline and Iodine</p> Signup and view all the answers

    Which of the following methods is NOT mentioned for early detection of ALA?

    <p>PCR</p> Signup and view all the answers

    Which stage of Acanthamoeba exhibits a characteristic single large nucleus with a centrally-located, densely staining nucleolus?

    <p>Trophozoite stage</p> Signup and view all the answers

    What is the typical mode of transmission of Acanthamoeba into the body?

    <p>Entry through the nasal passages to the lower respiratory tract</p> Signup and view all the answers

    Which clinical manifestation is associated with Acanthamoeba Keratitis?

    <p>Severe ocular pain and blurring of vision</p> Signup and view all the answers

    What is a possible complication of Acanthamoeba Keratitis?

    <p>Corneal ulceration</p> Signup and view all the answers

    How is the diagnosis of GAE (Granulomatous Amebic Encephalitis) typically confirmed?

    <p>Postmortem demonstration of trophozoites or cysts in tissues using histopathologic stains and microscopy</p> Signup and view all the answers

    Why is the diagnosis of GAE frequently missed?

    <p>Rarity of the disease and unfamiliarity of most physicians with the pathogen</p> Signup and view all the answers

    How do trophozoites of Naegleria fowleri infect humans or animals?

    <p>By migrating to the brain via olfactory nerves</p> Signup and view all the answers

    Which of the following is a preventive measure against Naegleria fowleri infection in endemic areas?

    <p>Avoiding immersion of the head and accidental inhalation of water</p> Signup and view all the answers

    How can Naegleria fowleri be effectively killed in swimming water?

    <p>Chlorination of water at 1 ppm or higher</p> Signup and view all the answers

    What is the mode of replication of Naegleria fowleri trophozoites?

    <p>Promitosis</p> Signup and view all the answers

    Where are flagellated forms of Naegleria fowleri occasionally found?

    <p>Cerebrospinal fluid (CSF)</p> Signup and view all the answers

    Which laboratory diagnostic method is typically used to detect Naegleria fowleri in patients?

    <p>CSF examination</p> Signup and view all the answers

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