Microscopic Diagnosis of Amebiasis

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18 Questions

What is the standard method for diagnosing Amebiasis?

Detection of antibodies using ELISA

Which method is used to differentiate Entamoeba spp from white blood cells?

Saline and Methylene Blue

What is more sensitive than Direct Fecal Smear (DFS) for diagnosing Amebiasis?

ELISA with PCR

Which method is not mentioned as a key diagnostic tool for Amebic Liver Abscess (ALA)?

Computerized tomography (CT scan)

Which method is specifically used to observe the nucleus and karyosome to differentiate E.histolytica from other species?

Saline and Iodine

Which of the following methods is NOT mentioned for early detection of ALA?

PCR

Which stage of Acanthamoeba exhibits a characteristic single large nucleus with a centrally-located, densely staining nucleolus?

Trophozoite stage

What is the typical mode of transmission of Acanthamoeba into the body?

Entry through the nasal passages to the lower respiratory tract

Which clinical manifestation is associated with Acanthamoeba Keratitis?

Severe ocular pain and blurring of vision

What is a possible complication of Acanthamoeba Keratitis?

Corneal ulceration

How is the diagnosis of GAE (Granulomatous Amebic Encephalitis) typically confirmed?

Postmortem demonstration of trophozoites or cysts in tissues using histopathologic stains and microscopy

Why is the diagnosis of GAE frequently missed?

Rarity of the disease and unfamiliarity of most physicians with the pathogen

How do trophozoites of Naegleria fowleri infect humans or animals?

By migrating to the brain via olfactory nerves

Which of the following is a preventive measure against Naegleria fowleri infection in endemic areas?

Avoiding immersion of the head and accidental inhalation of water

How can Naegleria fowleri be effectively killed in swimming water?

Chlorination of water at 1 ppm or higher

What is the mode of replication of Naegleria fowleri trophozoites?

Promitosis

Where are flagellated forms of Naegleria fowleri occasionally found?

Cerebrospinal fluid (CSF)

Which laboratory diagnostic method is typically used to detect Naegleria fowleri in patients?

CSF examination

Learn about the standard microscopic detection of Entamoeba trophozoites and cysts in stool samples for diagnosing amebiasis. Understand the different techniques used, such as examining fresh samples, using saline and methylene blue for differentiation, and observing nucleus characteristics for accurate diagnosis.

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