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Questions and Answers
Which type of microscopy is commonly used for observing bacteria such as treponemes and leptospires?
Which type of microscopy is commonly used for observing bacteria such as treponemes and leptospires?
- Phase-contrast microscopy
- Brightfield microscopy
- Darkfield microscopy (correct)
- Electron microscopy
Which type of microscopy allows examination of live unstained organisms?
Which type of microscopy allows examination of live unstained organisms?
- Fluorescence microscopy
- Electron microscopy
- Phase-contrast microscopy (correct)
- Brightfield microscopy
Which type of microscopy uses fluorochromes to stain specimens and excited molecules emit light of different wavelengths?
Which type of microscopy uses fluorochromes to stain specimens and excited molecules emit light of different wavelengths?
- Darkfield microscopy
- Phase-contrast microscopy
- Fluorescence microscopy (correct)
- Electron microscopy
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Study Notes
- Microscopes used in clinical practice are light microscopes.
- Compound light microscopes are the most common in microbiology.
- Electron microscopes provide higher magnification for observing small microorganisms.
- Brightfield microscopy is commonly used with stained preparations.
- Darkfield microscopy is used for observing bacteria such as treponemes and leptospires.
- Phase-contrast microscopy allows examination of live unstained organisms.
- Fluorescence microscopy uses fluorochromes to stain specimens.
- Excited molecules emit light of different wavelengths in fluorescence microscopy.
- Auramine differential staining is an application of fluorescence microscopy.
- Rapid diagnostic kits use fluorescent antibodies for identifying many pathogens.
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