Microscope Types and Parts

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Questions and Answers

What is the primary purpose of using a microscope?

  • To analyze color composition of specimens
  • To magnify small objects or fine details (correct)
  • To measure the weight of specimens
  • To create images of large objects

Which component of a microscope is used to secure the slide on the stage?

  • Iris diaphragm
  • Stage clips (correct)
  • Condenser
  • Coarse adjustment knob

What magnification do typical ocular lenses provide in an optical microscope?

  • 100X
  • 40X
  • 4X
  • 10X (correct)

Which part of the microscope connects the eyepiece to the objective lenses?

<p>Head (or body tube) (B)</p> Signup and view all the answers

Which knob should be used first when focusing the microscope under low power?

<p>Coarse adjustment knob (A)</p> Signup and view all the answers

What is the function of the iris diaphragm in a microscope?

<p>To control the amount of light reaching the specimen (D)</p> Signup and view all the answers

What does the revolving nosepiece on a microscope allow you to do?

<p>Switch between different objective lenses (B)</p> Signup and view all the answers

Which term describes the flat platform where slides are placed in a microscope?

<p>Stage (A)</p> Signup and view all the answers

Flashcards

Microscope

An instrument that magnifies small objects or details of larger ones.

Ocular lenses

Lenses you look through; typically magnify at 10X.

Objective lenses

Lenses with various magnifications mounted on a rotating nosepiece.

Revolving nosepiece

Rotating part that holds the objective lenses to switch between them.

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Coarse adjustment knob

The first knob used for larger focus adjustments, best under low power.

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Fine adjustment knob

Knob used for precise focusing, best under higher power.

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Stage

Flat platform where the microscope slide with the specimen is placed.

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Light source (illuminator)

Sends light upward through the specimen for visibility.

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Study Notes

Microscope Types

  • Light microscopes use optical lenses and light to produce magnified images.
  • These are commonly used to view specimens that aren't visible to the naked eye.

Light Microscope Parts

  • Ocular Lenses (Eyepieces): Provide magnification (typically 10X). May have a pointer for precise specimen location.
  • Objective Lenses: Attached to a revolving nosepiece. Varied magnification (e.g. 4X, 10X, 40X, 100X) allowing for different levels of magnification.
  • Head (or Body Tube): Connects eyepiece to the objective lenses.
  • Arm: Supports the body tube and connects it to the base.
  • Base: Supports the microscope and contains the light source.
  • Light Source (Illuminator): Provides light to illuminate the specimen. Older microscopes used mirrors for this purpose.
  • Condenser: Focuses light onto the specimen.
  • Iris Diaphragm: Adjusts the amount of light reaching the specimen through the condenser.
  • Stage: Holds the specimen slide.
  • Stage Clips: Secure the slide on the stage.
  • Coarse Adjustment Knob: Used for initial focusing adjustments, mainly for low magnification.
  • Fine Adjustment Knob: Used for fine-tuning focus, often needed for high magnification.
  • Revolving Nosepiece: Allows for changing between different objective lenses.

Magnification Calculation

  • Calculate total magnification by multiplying the magnification of the ocular lens by the magnification of the objective lens. (e.g., 10X ocular lens x 40X objective lens = 400X total magnification)

Electron Microscopes

  • Electron microscopes use a beam of electrons to create magnified images.
  • Two main types: Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM).

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