Microscope Slide Preparation

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What are microscope slides generally used for?

Pathology, histology, and cytology (correct)

Environmental studies and analysis

Chemical experiments and reactions

Physics experiments and measurements

How are slide surfaces treated to make them resistant to certain chemicals?

By coating with metal alloys

By exposing to UV radiation

By heating at high temperatures

With biological reagents (correct)

What is the purpose of permeabilization in sample treatment?

To attach samples to slides

To suspend the sample in a fluid on a slide

To dissolve cell membranes and allow more dye molecules to enter cells (correct)

To increase rigidity of samples

What is the purpose of squash slides in sample preparation?

For soft samples Signup and view all the answers

What is the purpose of staining in microscopy?

To enhance contrast in the microscopic image and define tissues, cells or organelles Signup and view all the answers

Apart from visualizing cells, what other purpose can stains serve in microscopy?

Following some metabolic processes and differentiating between live and dead cells in a sample Signup and view all the answers

Which stain can be used in both living and fixed cells?

DAPI Signup and view all the answers

Which stain is specifically used for apoptotic cells?

Ethidium bromide Signup and view all the answers

What is the purpose of adding a drop of stain in slide preparation?

To draw the stain through and color the cells Signup and view all the answers

What is the purpose of using methylene blue stain in the cell preparation process?

To enhance the contrast and visibility of the cells under the microscope Signup and view all the answers

Why is no coverslip needed in examining the blood smear after staining?

To avoid crushing the blood cells on the slide Signup and view all the answers

What is the purpose of rinsing the stained slide with distilled water?

To remove excess stain from the slide Signup and view all the answers

Why is Leishman stain referred to as a universal stain for peripheral blood smears?

It is suitable for staining blood samples from various species Signup and view all the answers

What is the purpose of adding yeast and sugar to warm water in the slide preparation process?

To activate the yeast for observation under the microscope Signup and view all the answers

Why is it recommended to observe the yeast under high magnifications?

To clearly visualize the details of the yeast cells Signup and view all the answers

What is the recommended initial magnification for viewing the yoghurt slide in a compound microscope?

4x or 10x Signup and view all the answers

Why is step 2 in the yoghurt slide preparation process considered optional?

The bacteria can still be seen without using the stain Signup and view all the answers

Match the following stains with their primary targets in microscopy:

Methylene blue = Acidic cell parts (like nucleus) Crystal violet = Bacterial cell walls Iodine = Starch and glycogen Eosin = RBCs, cytoplasm, membranes & extracellular structures Signup and view all the answers

Match the following stains with their primary usage in fluorescence microscopy:

Rhodamine = Fluorescent stain for proteins Safranin = A nuclear stain Wright's stain = Stains RBCs pink/red DAPI = Fluorescent nuclear stain for living or fixed cells Signup and view all the answers