Podcast
Questions and Answers
What are microscope slides generally used for?
What are microscope slides generally used for?
- Pathology, histology, and cytology (correct)
- Environmental studies and analysis
- Chemical experiments and reactions
- Physics experiments and measurements
How are slide surfaces treated to make them resistant to certain chemicals?
How are slide surfaces treated to make them resistant to certain chemicals?
- By coating with metal alloys
- By exposing to UV radiation
- By heating at high temperatures
- With biological reagents (correct)
What is the purpose of permeabilization in sample treatment?
What is the purpose of permeabilization in sample treatment?
- To attach samples to slides
- To suspend the sample in a fluid on a slide
- To dissolve cell membranes and allow more dye molecules to enter cells (correct)
- To increase rigidity of samples
What is the purpose of squash slides in sample preparation?
What is the purpose of squash slides in sample preparation?
What is the purpose of staining in microscopy?
What is the purpose of staining in microscopy?
Apart from visualizing cells, what other purpose can stains serve in microscopy?
Apart from visualizing cells, what other purpose can stains serve in microscopy?
Which stain can be used in both living and fixed cells?
Which stain can be used in both living and fixed cells?
Which stain is specifically used for apoptotic cells?
Which stain is specifically used for apoptotic cells?
What is the purpose of adding a drop of stain in slide preparation?
What is the purpose of adding a drop of stain in slide preparation?
What is the purpose of using methylene blue stain in the cell preparation process?
What is the purpose of using methylene blue stain in the cell preparation process?
Why is no coverslip needed in examining the blood smear after staining?
Why is no coverslip needed in examining the blood smear after staining?
What is the purpose of rinsing the stained slide with distilled water?
What is the purpose of rinsing the stained slide with distilled water?
Why is Leishman stain referred to as a universal stain for peripheral blood smears?
Why is Leishman stain referred to as a universal stain for peripheral blood smears?
What is the purpose of adding yeast and sugar to warm water in the slide preparation process?
What is the purpose of adding yeast and sugar to warm water in the slide preparation process?
Why is it recommended to observe the yeast under high magnifications?
Why is it recommended to observe the yeast under high magnifications?
What is the recommended initial magnification for viewing the yoghurt slide in a compound microscope?
What is the recommended initial magnification for viewing the yoghurt slide in a compound microscope?
Why is step 2 in the yoghurt slide preparation process considered optional?
Why is step 2 in the yoghurt slide preparation process considered optional?
Match the following stains with their primary targets in microscopy:
Match the following stains with their primary targets in microscopy:
Match the following stains with their primary usage in fluorescence microscopy:
Match the following stains with their primary usage in fluorescence microscopy:
Match the following stains with their primary usage in gel electrophoresis:
Match the following stains with their primary usage in gel electrophoresis:
Flashcards
Microscope slides
Microscope slides
Used for pathology, histology, and cytology studies.
Purpose of staining
Purpose of staining
Enhances contrast in microscopic images to define tissues, cells, or organelles.
Purpose of permeabilization
Purpose of permeabilization
To dissolve cell membranes, allowing more dye molecules to enter cells.
Methylene blue stain
Methylene blue stain
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No coverslip on blood smear
No coverslip on blood smear
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Rinsing stained slide
Rinsing stained slide
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Leishman stain as a universal stain
Leishman stain as a universal stain
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Yeast and sugar in water
Yeast and sugar in water
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High magnification for yeast
High magnification for yeast
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