Microscope Slide Preparation
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Questions and Answers

What are microscope slides generally used for?

  • Pathology, histology, and cytology (correct)
  • Environmental studies and analysis
  • Chemical experiments and reactions
  • Physics experiments and measurements

How are slide surfaces treated to make them resistant to certain chemicals?

  • By coating with metal alloys
  • By exposing to UV radiation
  • By heating at high temperatures
  • With biological reagents (correct)

What is the purpose of permeabilization in sample treatment?

  • To attach samples to slides
  • To suspend the sample in a fluid on a slide
  • To dissolve cell membranes and allow more dye molecules to enter cells (correct)
  • To increase rigidity of samples

What is the purpose of squash slides in sample preparation?

<p>For soft samples (A)</p> Signup and view all the answers

What is the purpose of staining in microscopy?

<p>To enhance contrast in the microscopic image and define tissues, cells or organelles (C)</p> Signup and view all the answers

Apart from visualizing cells, what other purpose can stains serve in microscopy?

<p>Following some metabolic processes and differentiating between live and dead cells in a sample (A)</p> Signup and view all the answers

Which stain can be used in both living and fixed cells?

<p>DAPI (B)</p> Signup and view all the answers

Which stain is specifically used for apoptotic cells?

<p>Ethidium bromide (B)</p> Signup and view all the answers

What is the purpose of adding a drop of stain in slide preparation?

<p>To draw the stain through and color the cells (D)</p> Signup and view all the answers

What is the purpose of using methylene blue stain in the cell preparation process?

<p>To enhance the contrast and visibility of the cells under the microscope (A)</p> Signup and view all the answers

Why is no coverslip needed in examining the blood smear after staining?

<p>To avoid crushing the blood cells on the slide (B)</p> Signup and view all the answers

What is the purpose of rinsing the stained slide with distilled water?

<p>To remove excess stain from the slide (C)</p> Signup and view all the answers

Why is Leishman stain referred to as a universal stain for peripheral blood smears?

<p>It is suitable for staining blood samples from various species (A)</p> Signup and view all the answers

What is the purpose of adding yeast and sugar to warm water in the slide preparation process?

<p>To activate the yeast for observation under the microscope (C)</p> Signup and view all the answers

Why is it recommended to observe the yeast under high magnifications?

<p>To clearly visualize the details of the yeast cells (B)</p> Signup and view all the answers

What is the recommended initial magnification for viewing the yoghurt slide in a compound microscope?

<p>4x or 10x (B)</p> Signup and view all the answers

Why is step 2 in the yoghurt slide preparation process considered optional?

<p>The bacteria can still be seen without using the stain (A)</p> Signup and view all the answers

Match the following stains with their primary targets in microscopy:

<p>Methylene blue = Acidic cell parts (like nucleus) Crystal violet = Bacterial cell walls Iodine = Starch and glycogen Eosin = RBCs, cytoplasm, membranes &amp; extracellular structures</p> Signup and view all the answers

Match the following stains with their primary usage in fluorescence microscopy:

<p>Rhodamine = Fluorescent stain for proteins Safranin = A nuclear stain Wright's stain = Stains RBCs pink/red DAPI = Fluorescent nuclear stain for living or fixed cells</p> Signup and view all the answers

Match the following stains with their primary usage in gel electrophoresis:

<p>Ethidium bromide = Stains apoptotic cells Coomassie blue = Stains proteins and is often used in gel electrophoresis.</p> Signup and view all the answers

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