20 Questions
What are microscope slides generally used for?
Pathology, histology, and cytology
How are slide surfaces treated to make them resistant to certain chemicals?
With biological reagents
What is the purpose of permeabilization in sample treatment?
To dissolve cell membranes and allow more dye molecules to enter cells
What is the purpose of squash slides in sample preparation?
For soft samples
What is the purpose of staining in microscopy?
To enhance contrast in the microscopic image and define tissues, cells or organelles
Apart from visualizing cells, what other purpose can stains serve in microscopy?
Following some metabolic processes and differentiating between live and dead cells in a sample
Which stain can be used in both living and fixed cells?
DAPI
Which stain is specifically used for apoptotic cells?
Ethidium bromide
What is the purpose of adding a drop of stain in slide preparation?
To draw the stain through and color the cells
What is the purpose of using methylene blue stain in the cell preparation process?
To enhance the contrast and visibility of the cells under the microscope
Why is no coverslip needed in examining the blood smear after staining?
To avoid crushing the blood cells on the slide
What is the purpose of rinsing the stained slide with distilled water?
To remove excess stain from the slide
Why is Leishman stain referred to as a universal stain for peripheral blood smears?
It is suitable for staining blood samples from various species
What is the purpose of adding yeast and sugar to warm water in the slide preparation process?
To activate the yeast for observation under the microscope
Why is it recommended to observe the yeast under high magnifications?
To clearly visualize the details of the yeast cells
What is the recommended initial magnification for viewing the yoghurt slide in a compound microscope?
4x or 10x
Why is step 2 in the yoghurt slide preparation process considered optional?
The bacteria can still be seen without using the stain
Match the following stains with their primary targets in microscopy:
Methylene blue = Acidic cell parts (like nucleus) Crystal violet = Bacterial cell walls Iodine = Starch and glycogen Eosin = RBCs, cytoplasm, membranes & extracellular structures
Match the following stains with their primary usage in fluorescence microscopy:
Rhodamine = Fluorescent stain for proteins Safranin = A nuclear stain Wright's stain = Stains RBCs pink/red DAPI = Fluorescent nuclear stain for living or fixed cells
Match the following stains with their primary usage in gel electrophoresis:
Ethidium bromide = Stains apoptotic cells Coomassie blue = Stains proteins and is often used in gel electrophoresis.
Test your knowledge of microscope slide preparation used in pathology, histology, and cytology. Learn about the different types of microscope slides and how they are treated with biological reagents for various purposes.
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