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Questions and Answers
What part of the microscope controls the amount of light?
What part of the microscope controls the amount of light?
Which part of the microscope is responsible for gathering light and concentrating it into a cone of light?
Which part of the microscope is responsible for gathering light and concentrating it into a cone of light?
What is used for focusing on the scanner, or low-power objective (LPO)?
What is used for focusing on the scanner, or low-power objective (LPO)?
What part of the microscope provides a support for the microscope?
What part of the microscope provides a support for the microscope?
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Match the following microscope objectives with their magnification:
Match the following microscope objectives with their magnification:
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The ______ is the platform on which the slide is positioned.
The ______ is the platform on which the slide is positioned.
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What is the purpose of stage clips?
What is the purpose of stage clips?
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What is the magnification of a compound microscope's low-power objective (LPO)?
What is the magnification of a compound microscope's low-power objective (LPO)?
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What type of microscope provides a three-dimensional view of the specimen?
What type of microscope provides a three-dimensional view of the specimen?
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The cell membrane regulates the flow of substances between the cell and its surroundings.
The cell membrane regulates the flow of substances between the cell and its surroundings.
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What is the function of the nucleus within a cell?
What is the function of the nucleus within a cell?
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What is the substance outside the nucleus called?
What is the substance outside the nucleus called?
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Methylene blue is used to stain human cheek cells to get a clearer view of the cellular structures.
Methylene blue is used to stain human cheek cells to get a clearer view of the cellular structures.
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Which of the following is NOT a characteristic of a frog egg cell?
Which of the following is NOT a characteristic of a frog egg cell?
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The distinct nucleus of red blood cells in frogs are more evident because they need a lot of oxygen as amphibians.
The distinct nucleus of red blood cells in frogs are more evident because they need a lot of oxygen as amphibians.
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The white blood cells of frogs have no distinct nucleus.
The white blood cells of frogs have no distinct nucleus.
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What is the main function of the white blood cells in humans, besides fighting infections?
What is the main function of the white blood cells in humans, besides fighting infections?
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The sperm cell can move towards the egg and fertilize it due to the flagellum.
The sperm cell can move towards the egg and fertilize it due to the flagellum.
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What is the process of cell division called?
What is the process of cell division called?
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What is the resting phase of the cell cycle called?
What is the resting phase of the cell cycle called?
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During the G1 phase, the cell undergoes preparation for division, including growth and duplication of cytoplasmic structures.
During the G1 phase, the cell undergoes preparation for division, including growth and duplication of cytoplasmic structures.
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What is the name of the phase where DNA synthesis takes place?
What is the name of the phase where DNA synthesis takes place?
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What phase of the cell cycle involves the preparation for the onset of mitosis?
What phase of the cell cycle involves the preparation for the onset of mitosis?
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What is the genetic material called in the cell during the G2 phase?
What is the genetic material called in the cell during the G2 phase?
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The chromosomes align at the equatorial region/metaphase during metaphase.
The chromosomes align at the equatorial region/metaphase during metaphase.
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What is the name given to replicated chromosomes, which are shorter and more condensed, and referred to as sister?
What is the name given to replicated chromosomes, which are shorter and more condensed, and referred to as sister?
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What are the protein complexes attached to the centromere of chromosomes called?
What are the protein complexes attached to the centromere of chromosomes called?
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What occurs during anaphase?
What occurs during anaphase?
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What is the term used for the distance traveled by the chromatids during anaphase?
What is the term used for the distance traveled by the chromatids during anaphase?
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The constriction of the plasma membrane at the equatorial plate during telophase leads to the formation of two daughter cells.
The constriction of the plasma membrane at the equatorial plate during telophase leads to the formation of two daughter cells.
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Early telophase is characterized by the reappearance of the nuclear membrane and the nucleolus.
Early telophase is characterized by the reappearance of the nuclear membrane and the nucleolus.
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The chromosomes uncoil and assume a threadlike appearance during telophase.
The chromosomes uncoil and assume a threadlike appearance during telophase.
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The asters and mitotic spindles disappear during late telophase.
The asters and mitotic spindles disappear during late telophase.
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The cleavage furrow becomes more constricted during late telophase, leading to the formation of two daughter cells.
The cleavage furrow becomes more constricted during late telophase, leading to the formation of two daughter cells.
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What are the cells called that make up the embryo?
What are the cells called that make up the embryo?
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The gastrula stage is characterized by the presence of ectoderm, mesoderm and endoderm
The gastrula stage is characterized by the presence of ectoderm, mesoderm and endoderm
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What is the cavity in the blastula stage called?
What is the cavity in the blastula stage called?
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What tissue forms the outer covering of external surfaces?
What tissue forms the outer covering of external surfaces?
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Epithelial tissues are avascular, meaning they lack blood vessels.
Epithelial tissues are avascular, meaning they lack blood vessels.
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Epithelial tissues are innervated by nerves.
Epithelial tissues are innervated by nerves.
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What provides structural support for epithelial tissue?
What provides structural support for epithelial tissue?
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Which type of epithelium covers the external surfaces of digestive organs, lungs, and heart?
Which type of epithelium covers the external surfaces of digestive organs, lungs, and heart?
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Study Notes
Microscope Parts and Functions
- Iris Diaphragm: Controls the amount of light
- Condenser: Gathers and concentrates light onto the specimen
- Light Source/Illuminator: Reflects light through the specimen
- Coarse Adjustment Knob: Used for initial focusing (scanner or low power objective)
- Fine Adjustment Knob: Used for final focusing
- Base: Supports the microscope
- Calculating Calibration: Methods for comparison measurements using microscopes.
- Stage Micrometer: Used for calibration of ocular micrometer
- Ocular/Eyepiece: Contains a lens (often 10x magnification) to aid in locating objects
- Arm Handle: Mechanical attachment for other parts
- Revolving Nose Piece: Holds and shifts objectives
- Objectives: Contain lenses for magnification (Scanner: 4x, Low Power: 10x, High Power: 40x, Oil Immersion: 100x)
- Stage: Platform where the slide is positioned
- Stage Clips: Hold the slide in place
- Ocular Micrometer: Used for measuring specimens
Animal Cell Structure
- Cell Membrane: Regulates the flow of substances between the cell and surroundings
- Nucleus: Usually spherical or ovoid, contains genetic material
- Cytoplasm: Substance outside the nucleus, contains organelles like mitochondria and ribosomes
Comparison of Microscopes
- Magnification: A compound microscope has a higher magnification power than a dissecting microscope.
- Compound microscope: 4x, 10x, 40x, 100x
- Dissecting microscope: 5x, 50x
- Dimensions: 2D for compound, 3D for dissecting
Animal Cell Activity (Human Cheek Cells)
- Utilize methylene blue to visualize cellular structures in cheek cells
Animal Cells Activity (Frog Stomach)
- Not included in the provided text in this context
Cell Division/Mitosis
- Interphase: Resting and preparing for division phase
- Prophase: Chromatin fibers condense forming chromosomes.
- Metaphase: Chromosomes align at the equatorial region.
- Anaphase: Sister chromatids are pulled apart to opposite poles.
- Telophase: Nuclear membrane and nucleolus reappear, chromosomes uncoil
Animal Development (Cleavage)
- Early Cleavage: Cells divide, becoming blastomeres.
- Late Cleavage: Small blastomeres = micromeres, Large blastomeres = macromeres. -Cells arranged at animal and vegetal poles.
- Gastrula: Stage of development after cleavage.
- Blastula: Cell stages present after cleavage
Tissues (Epithelial Tissues)
- Epithelial Tissue: Covers external surfaces and lines internal surfaces, forms secretory units of glands (exocrine, endocrine).
- Functions: Protection, excretion, special functions for sensory organs
- Characteristics: Closely packed cells, highly cellular, avascular, presence of basement membrane.
- Types of Epithelial Tissue: Simple squamous, Simple cuboidal, Simple columnar, Stratified squamous, Pseudostratified columnar and transitional
Connective Tissues (Fibers)
- Ground Substance: Homogenous, transparent, hydrated gel
- Fibers: Provide tensile strength and flexibility, elastic fibers provide resiliency
- Types of Connective Tissues: Elastic Fibers , Reticular Fibers, Collagen Fibers
Blood
-
Red Blood Cells: Unstained pale yellow or greenish yellow color, pink when stained routinely, Rouleaux formation (RBCs adhere to each other)
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White Blood Cells: Granulocytes (Neutrophils, Eosinophils, Basophils) and Agranulocytes (Lymphocytes, Monocytes), different types have different functions in immunity and inflammation (ie.. neutrophils - for bacteria defense, eosinophils - for parasites, and basophils - release histamine)
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Fat Cells (Adipocytes): Store lipids (SIGNET RING CELLS- special type of adipocyte)
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White Adipose Tissue: Serves as energy source, forms insulation and cushioning
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Brown Adipose Tissue: Generates heat
Cartilage
- Hyaline Cartilage: Most abundant, underlies articular cartilage, found in trachea and bronchi.
- Elastic Cartilage: More flexible, found in outer ear
- Fibro Cartilage: Extremely strong, found in intervertebral discs
Bone
- Special form of connective tissue
- Minerals deposited in the matrix
Muscle Tissue
- Smooth Muscle: Involuntary, found in internal organs
- Cardiac Muscle: Involuntary, found in the heart
- Skeletal Muscle: Voluntary, found in muscles attached to bones
Nervous Tissue
- Neural Tissue: Found in the brain, spinal cord and nerves to transmit impulses
- Nerve Cells: Specialized to transmit signals (communication)
- Neuroglia: Supports and protect nerve cells
Other Tissues
- Frog Skin: layers of stratified epithelium. Outer layer: Stratum Corneum (dead, flattened cells) ; Middle layer: Stratum Germinativum (multiple layers of cuboidal to columnar cells).
Spinal Cord
- Central Canal: Inner structure
- Grey Matter: Contains nerve cell bodies
- White Matter: Contains nerve fibers
- Dura Mater: Protective outer layer
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Description
This quiz covers the various parts of a microscope and their specific functions, including the iris diaphragm, coarse adjustment knob, and light source. Understanding these components is essential for effective microscopy and specimen analysis. Test your knowledge on the key features and their roles in microscopy.