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Microscope Basics: Magnification and Lens Care

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Questions and Answers

What is the primary purpose of the coarse focus knob in a microscope?

To secure the microscope slide on the stage
To fine-tune the focus of the microscope
To adjust the amount of light entering the microscope
To move the objective lens upward or downward (correct)

Why should the objective lens not touch the microscope slide?

To prevent the spread of contamination
To maintain a sterile environment
To avoid damaging the lens or slide (correct)
To ensure proper illumination of the specimen

What is the purpose of the fine adjustment in a microscope?

To adjust the amount of light entering the microscope
To secure the microscope slide on the stage
To fine-tune the focus of the microscope (correct)
To move the stage up or down

What should you do if you cannot focus on your specimen with the current objective lens?

Repeat steps 4 through 7 with the higher power objective lens in place (A) Signup and view all the answers

Why should you adjust the mirror, illuminator, or diaphragm during microscope usage?

To achieve the greatest amount of light for the clearest image (B) Signup and view all the answers

What should you do after focusing on your specimen with the current objective lens?

Move the microscope slide around to center the image (B) Signup and view all the answers

What is the recommended practice for lens ports when not in use?

Use the port cover or sealing tape (B) Signup and view all the answers

What is the formula to calculate the total magnification of a microscope?

Multiply the power of the eyepiece by the power of the objective lens (C) Signup and view all the answers

What is the correct way to view an object through a monocular microscope?

Close one eye and keep the other eye open (D) Signup and view all the answers

What is the term for when the magnification is increased but the detail is not increased due to resolution limitations?

Empty magnification (B) Signup and view all the answers

What happens when you move the slide to the right while using a monocular microscope?

The image goes to the left (B) Signup and view all the answers

What should be used to clean the lenses of a monocular microscope?

Special lens paper (C) Signup and view all the answers

What is the best practice when observing a specimen with a microscope?

Start with the lowest power and progress to higher powers (D) Signup and view all the answers

Why is it recommended to use a higher magnification objective rather than a lower power objective with a high-powered eyepiece?

It results in higher quality views (B) Signup and view all the answers

What is the primary enemy of a monocular microscope?

Dust (D) Signup and view all the answers

What should be done daily to maintain a monocular microscope?

Record any damage or malfunction in the daily microscopy maintenance sheet (C) Signup and view all the answers

What is the unit of measurement for the field of view in a microscope?

Millimeters (B) Signup and view all the answers

What should be done at the end of the day to protect the microscope?

Turn off the power and unplug the microscope (C) Signup and view all the answers

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