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Questions and Answers
What is the overall magnification when using an eyepiece magnification of $X10$ and an objective lens magnification of $X40$?
What is the overall magnification when using an eyepiece magnification of $X10$ and an objective lens magnification of $X40$?
- 400 (correct)
- 40
- 100
- 1000
What is the main factor that determines the resolving power of a microscope?
What is the main factor that determines the resolving power of a microscope?
- Size and curvature of the lens
- Magnification power
- Type of microscope
- Wavelength of the light and optical quality of the lenses (correct)
Which type of microscope is known for its ability to visualize living cells and biological molecules?
Which type of microscope is known for its ability to visualize living cells and biological molecules?
- Fluorescence microscope (correct)
- Bright-field microscope
- Transmission electron microscope
- Scanning electron microscope
What is the function of the objective lens in a compound microscope?
What is the function of the objective lens in a compound microscope?
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What is the role of the dark-field microscope in microscopy?
What is the role of the dark-field microscope in microscopy?
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Study Notes
Magnification in Microscopy
- Overall magnification is calculated by multiplying the eyepiece and objective lens magnifications.
- For an eyepiece magnification of 10x and an objective lens magnification of 40x, overall magnification is 400x.
Resolving Power
- The resolving power of a microscope is mainly determined by the wavelength of light used and the numerical aperture of the objective lens.
- Higher resolving power allows for clearer and more detailed images of small structures.
Types of Microscopes
- The phase-contrast microscope is known for its ability to visualize living cells and biological molecules without staining.
- This microscope enhances contrast in transparent specimens, making it suitable for biological applications.
Function of Objective Lens
- The objective lens in a compound microscope serves to gather light from the specimen, magnifying the image.
- It is critical for resolution and image clarity, with various lenses available for different magnifications and applications.
Role of Dark-Field Microscope
- The dark-field microscope enhances contrast in unstained, transparent specimens by illuminating them with scattered light.
- This technique enables the observation of living cells and offers a unique perspective on biological structures.
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