Microscope and Light Microscopy
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Questions and Answers

What is the primary function of a microscope?

  • To magnify an image and allow visualization of greater detail than the unaided eye. (correct)
  • To assess surface properties of cells and other biological objects.
  • To provide light to illuminate objects.
  • To quantify tissue mass.

Which of the following is not a type of light microscopy mentioned in the text?

  • Electron (correct)
  • Bright-field
  • Simple
  • Compound

What is the role of the condenser lens in a bright-field microscope?

  • To provide the light source.
  • To hold the microscope slide.
  • To magnify the image 10x.
  • To collect and focus light on the object to be studied. (correct)

What is the key difference between the interference microscope and the differential interference microscope (Nomarski)?

<p>The interference microscope quantifies tissue mass, while the Nomarski microscope assesses surface properties. (B)</p> Signup and view all the answers

What is the primary advantage of using a phase-contrast microscope?

<p>It enables examination of unstained cells and tissues. (D)</p> Signup and view all the answers

What is the purpose of the ocular lens (eyepiece) in a bright-field microscope?

<p>To magnify the image 10x and project it to the viewer. (D)</p> Signup and view all the answers

What is the key difference between polyclonal and monoclonal antibodies in immunocytochemical staining procedures?

<p>Polyclonal antibodies are directed against different types of epitopes, while monoclonal antibodies are binding-specific. (D)</p> Signup and view all the answers

What is the primary purpose of using a dark-field microscope clinically?

<p>To examine autoradiographs and demonstrate specific bacteria (D)</p> Signup and view all the answers

Which microscopy technique is best suited for visualizing the smallest resolvable distance between points?

<p>Atomic force microscopy (D)</p> Signup and view all the answers

What is the key difference between a direct fluorescence assay (dFA) and an indirect fluorescence assay?

<p>A positive dFA test uses one antibody to detect an antigen, while a positive indirect test uses two antibodies to detect an antigen. (A)</p> Signup and view all the answers

Which microscopy technique is best suited for examining autoradiographs and demonstrating specific bacteria?

<p>Dark-field microscopy (C)</p> Signup and view all the answers

What is the primary advantage of using monoclonal antibodies over polyclonal antibodies in immunocytochemical staining procedures?

<p>Monoclonal antibodies are more specific to a single epitope, while polyclonal antibodies are directed against different types of epitopes. (C)</p> Signup and view all the answers

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