Podcast
Questions and Answers
Who is credited with being the first person known to have observed living microbes?
Who is credited with being the first person known to have observed living microbes?
- Robert Hooke
- Alexander Fleming
- Antonie van Leeuwenhoek (correct)
- Louis Pasteur
What did Antonie van Leeuwenhoek observe in tooth tartar and feces?
What did Antonie van Leeuwenhoek observe in tooth tartar and feces?
- Viruses
- Protozoa
- Bacteria (correct)
- Fungi
Why did Antonie van Leeuwenhoek keep his microscopes and method of observing 'animalcules' to himself?
Why did Antonie van Leeuwenhoek keep his microscopes and method of observing 'animalcules' to himself?
- Because he wanted to sell his microscopes
- To protect his homemade microscopes (correct)
- Because he was too busy to share his knowledge
- To prevent others from learning about microbes
Where can directions for making a replica of van Leeuwenhoek's microscope be found?
Where can directions for making a replica of van Leeuwenhoek's microscope be found?
What is the primary purpose of Exercises 1 and 2 in the context of the text?
What is the primary purpose of Exercises 1 and 2 in the context of the text?
What is emphasized as a critical part of any science in the context of the text?
What is emphasized as a critical part of any science in the context of the text?
What is the most logical way to eliminate chromatic aberrations?
What is the most logical way to eliminate chromatic aberrations?
What is the cause of spherical aberration in a microscope lens?
What is the cause of spherical aberration in a microscope lens?
What is the purpose of using filters, usually blue, in a microscope?
What is the purpose of using filters, usually blue, in a microscope?
What should be done before using the oil immersion lens in a microscope?
What should be done before using the oil immersion lens in a microscope?
How can spherical aberrations be minimized in a microscope?
How can spherical aberrations be minimized in a microscope?
What is the purpose of using the iris diaphragm in a microscope?
What is the purpose of using the iris diaphragm in a microscope?
What should be done to achieve good contrast when using the low-power lens in a microscope?
What should be done to achieve good contrast when using the low-power lens in a microscope?
What is the most effective method of eliminating chromatic aberrations in a microscope?
What is the most effective method of eliminating chromatic aberrations in a microscope?
What should be done after use to maintain the microscope?
What should be done after use to maintain the microscope?
What is recommended to avoid eyestrain when using a microscope?
What is recommended to avoid eyestrain when using a microscope?
Why is it important to carry the microscope with both hands?
Why is it important to carry the microscope with both hands?
What is the function of the coarse-adjustment knob in a microscope?
What is the function of the coarse-adjustment knob in a microscope?
In a compound microscope, what is the function of the oil immersion lens?
In a compound microscope, what is the function of the oil immersion lens?
What is the total magnification of an object when using an ocular lens with a magnification of 10* and an objective lens with a magnification of 40*?
What is the total magnification of an object when using an ocular lens with a magnification of 10* and an objective lens with a magnification of 40*?
What is resolving power in microscopy?
What is resolving power in microscopy?
Which part of the microscope controls the amount of light entering the condenser?
Which part of the microscope controls the amount of light entering the condenser?
What is the function of the ocular lens in a microscope?
What is the function of the ocular lens in a microscope?
In a compound light microscope, what is used for focusing with the high-power and oil immersion lenses?
In a compound light microscope, what is used for focusing with the high-power and oil immersion lenses?
What is the purpose of immersion oil in microscopy?
What is the purpose of immersion oil in microscopy?
What is numerical aperture (N.A.) in microscopy?
What is numerical aperture (N.A.) in microscopy?
What does resolving power in microscopy depend on?
What does resolving power in microscopy depend on?
What is used to adjust current to lamp in a compound microscope?
What is used to adjust current to lamp in a compound microscope?
What is the purpose of the exercise described in the text?
What is the purpose of the exercise described in the text?
What is the advantage of having parfocal microscope objectives?
What is the advantage of having parfocal microscope objectives?
Which objective focuses closest to the slide when it is in focus?
Which objective focuses closest to the slide when it is in focus?
What would occur if water were accidentally used in place of immersion oil?
What would occur if water were accidentally used in place of immersion oil?
Which controls on the microscope affect the amount of light reaching the ocular lens?
Which controls on the microscope affect the amount of light reaching the ocular lens?
What are the advantages of the low-power objective over the oil immersion objective for viewing fungi or algae?
What are the advantages of the low-power objective over the oil immersion objective for viewing fungi or algae?
In what situation would microorganisms become apparent when switching to 1000* magnification?
In what situation would microorganisms become apparent when switching to 1000* magnification?
What is the purpose of moving the high-dry lens out of position and placing a drop of immersion oil on the slide?
What is the purpose of moving the high-dry lens out of position and placing a drop of immersion oil on the slide?
What does increased magnification do to the depth of field?
What does increased magnification do to the depth of field?
What are two ways in which you can enhance the resolving power?
What are two ways in which you can enhance the resolving power?
What would occur if one Bacillus cell measures 2 μm long, how many Bacillus cells could fit end-to-end across a field which has a diameter of 2 mm under low power?
What would occur if one Bacillus cell measures 2 μm long, how many Bacillus cells could fit end-to-end across a field which has a diameter of 2 mm under low power?
What is the purpose of handling the coverslip carefully by its edges when preparing a wet-mount slide?
What is the purpose of handling the coverslip carefully by its edges when preparing a wet-mount slide?
What is the main difference between Brownian movement and true motility in microorganisms?
What is the main difference between Brownian movement and true motility in microorganisms?
What is the advantage of using a hanging-drop slide over a wet-mount slide for observing motile microorganisms?
What is the advantage of using a hanging-drop slide over a wet-mount slide for observing motile microorganisms?
What is one reason why direct examination of living microorganisms is very useful?
What is one reason why direct examination of living microorganisms is very useful?
What effect does alcohol or Gram’s iodine have on motile microorganisms in a wet-mount slide?
What effect does alcohol or Gram’s iodine have on motile microorganisms in a wet-mount slide?
What technique would be most effective for observing bacteria in a wet-mount slide?
What technique would be most effective for observing bacteria in a wet-mount slide?
What should be done before discarding slides and coverslips in the disinfectant jar?
What should be done before discarding slides and coverslips in the disinfectant jar?
What is the purpose of placing a ring of petroleum jelly around the edge of a coverslip in the hanging-drop procedure?
What is the purpose of placing a ring of petroleum jelly around the edge of a coverslip in the hanging-drop procedure?
What is the significance of using brightfield microscopy to observe unstained microorganisms in wet mounts and hanging drop slides?
What is the significance of using brightfield microscopy to observe unstained microorganisms in wet mounts and hanging drop slides?
What does motility look like in microorganisms as compared to Brownian movement?
What does motility look like in microorganisms as compared to Brownian movement?
What type of microscope lens should be used to observe bacteria in a wet-mount slide?
What type of microscope lens should be used to observe bacteria in a wet-mount slide?
What is one advantage of using a petroleum jelly seal in the hanging-drop procedure?
What is one advantage of using a petroleum jelly seal in the hanging-drop procedure?
What type of microorganism is most likely to be observed in a wet mount of urine if flagellated, nucleated cells are seen?
What type of microorganism is most likely to be observed in a wet mount of urine if flagellated, nucleated cells are seen?
In a fixed, stained slide, when cells are not visible until the oil immersion objective is used, what type of microorganism is most likely?
In a fixed, stained slide, when cells are not visible until the oil immersion objective is used, what type of microorganism is most likely?
In a wet mount of scrapings, if long filaments composed of many cells are observed, which type of microorganism is most likely?
In a wet mount of scrapings, if long filaments composed of many cells are observed, which type of microorganism is most likely?
When observing microorganisms through a microscope, which optical system is used to ensure adequate light for viewing?
When observing microorganisms through a microscope, which optical system is used to ensure adequate light for viewing?
What was the invention by Zacharias Janssen that paved the way for the examination of microorganisms?
What was the invention by Zacharias Janssen that paved the way for the examination of microorganisms?
What type of microscope is used most often to show dark objects in a bright field when observing microorganisms?
What type of microscope is used most often to show dark objects in a bright field when observing microorganisms?
What is the purpose of the condenser’s iris diaphragm in a compound microscope?
What is the purpose of the condenser’s iris diaphragm in a compound microscope?
What part of the microscope holds the objective lenses and allows for easy switching between different magnifications?
What part of the microscope holds the objective lenses and allows for easy switching between different magnifications?
What did Antoni van Leeuwenhoek use to observe microorganisms that were too small to be seen with the naked eye?
What did Antoni van Leeuwenhoek use to observe microorganisms that were too small to be seen with the naked eye?
What did Antoni van Leeuwenhoek observe between his teeth even though he kept them very clean?
What did Antoni van Leeuwenhoek observe between his teeth even though he kept them very clean?
What was Antoni van Leeuwenhoek's observation regarding the motion and behavior of the small living animals he observed?
What was Antoni van Leeuwenhoek's observation regarding the motion and behavior of the small living animals he observed?
According to van Leeuwenhoek's observations, what were the small living animals he perceived to be doing?
According to van Leeuwenhoek's observations, what were the small living animals he perceived to be doing?
