Micropropagation and Tissue Culture Quiz

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Questions and Answers

What is the primary purpose of micropropagation?

  • To create genetically diverse plants
  • To produce clones of a plant (correct)
  • To enhance fruit size
  • To induce seed production

Aseptic conditions are important in tissue culture to prevent the introduction of unwanted microorganisms.

True (A)

What types of plant materials can be used as explants in micropropagation?

Leaves, roots, or buds

The hormone used to induce root development in tissue culture is _____ or cytokinins.

<p>auxin</p> Signup and view all the answers

Match the following concepts with their descriptions:

<p>Micropropagation = Using tissue culture to produce clones Callus = A mass of undifferentiated plant cells Agar = A gel-like medium used for plant culture Genetic diversity = Variation in genetic characteristics within a population</p> Signup and view all the answers

Which of the following is a benefit of micropropagation?

<p>Faster plant production (B)</p> Signup and view all the answers

Cloning through tissue culture can produce seedless fruit.

<p>True (A)</p> Signup and view all the answers

What is the role of glucose in the tissue culture medium?

<p>To provide energy for aerobic respiration</p> Signup and view all the answers

Micropropagation can lead to low _____ diversity, making plants more vulnerable to diseases.

<p>genetic</p> Signup and view all the answers

What is usually sterilized before beginning tissue culture?

<p>The bench and equipment (C)</p> Signup and view all the answers

Flashcards

Micropropagation

A technique where small pieces of plant tissue are grown in a sterile, nutrient-rich medium to produce clones of the original plant.

Tissue Culture

Refers to the sterilized container where small plant tissues are grown in micropropagation.

Explant

The small piece of plant tissue used in micropropagation.

Agar

A solid media containing nutrients like glucose and amino acids, used to support the growth of plant explants.

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Callus Formation

The process where cells in an explant divide and multiply to form a mass of undifferentiated cells.

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Plant Hormones

Plant hormones, like auxin and cytokinins, that regulate cell growth and differentiation in micropropagation.

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Root Induction

The manipulation of plant hormones to induce root development in the callus.

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Advantages of Micropropagation

The advantages of micropropagation include rapid and consistent production of desirable plant characteristics.

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Disadvantages of Micropropagation

A drawback of micropropagation is that it leads to a limited genetic diversity in the population.

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Seedless Fruit Production

Seeds are not needed in micropropagation, so it is possible to produce seedless fruits through tissue culture.

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Study Notes

Micropropagation and Tissue Culture

  • Micropropagation uses tissue culture to grow plants from cells.
  • Aseptic conditions are crucial, sterilizing the workspace and equipment to prevent competing microorganisms.
  • Plant material (explants) are taken, often from leaves, roots, or buds, due to their active mitosis.
  • Explants are sterilized using bleach or alcohol.
  • Explants are placed on agar containing nutrients like glucose (for respiration) and amino acids (for protein synthesis).
  • Cell division (mitosis) forms a callus.
  • The callus can be subdivided to create more calluses.
  • Hormones (auxin or cytokines) can be used to induce root formation or other desired development.
  • Plantlets are transitioned to soil or water, eventually to a greenhouse.

Advantages of Micropropagation

  • Preserves desirable traits in offspring (like taste, quantity of fruit).
  • Faster production than growing from seeds.
  • Enables seedless fruit production.

Disadvantages of Micropropagation

  • Low genetic diversity.
  • Increased susceptibility to disease as clones share the same genetic information.

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