Podcast
Questions and Answers
What is the primary purpose of micropropagation?
What is the primary purpose of micropropagation?
- To create genetically diverse plants
- To produce clones of a plant (correct)
- To enhance fruit size
- To induce seed production
Aseptic conditions are important in tissue culture to prevent the introduction of unwanted microorganisms.
Aseptic conditions are important in tissue culture to prevent the introduction of unwanted microorganisms.
True (A)
What types of plant materials can be used as explants in micropropagation?
What types of plant materials can be used as explants in micropropagation?
Leaves, roots, or buds
The hormone used to induce root development in tissue culture is _____ or cytokinins.
The hormone used to induce root development in tissue culture is _____ or cytokinins.
Match the following concepts with their descriptions:
Match the following concepts with their descriptions:
Which of the following is a benefit of micropropagation?
Which of the following is a benefit of micropropagation?
Cloning through tissue culture can produce seedless fruit.
Cloning through tissue culture can produce seedless fruit.
What is the role of glucose in the tissue culture medium?
What is the role of glucose in the tissue culture medium?
Micropropagation can lead to low _____ diversity, making plants more vulnerable to diseases.
Micropropagation can lead to low _____ diversity, making plants more vulnerable to diseases.
What is usually sterilized before beginning tissue culture?
What is usually sterilized before beginning tissue culture?
Flashcards
Micropropagation
Micropropagation
A technique where small pieces of plant tissue are grown in a sterile, nutrient-rich medium to produce clones of the original plant.
Tissue Culture
Tissue Culture
Refers to the sterilized container where small plant tissues are grown in micropropagation.
Explant
Explant
The small piece of plant tissue used in micropropagation.
Agar
Agar
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Callus Formation
Callus Formation
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Plant Hormones
Plant Hormones
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Root Induction
Root Induction
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Advantages of Micropropagation
Advantages of Micropropagation
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Disadvantages of Micropropagation
Disadvantages of Micropropagation
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Seedless Fruit Production
Seedless Fruit Production
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Study Notes
Micropropagation and Tissue Culture
- Micropropagation uses tissue culture to grow plants from cells.
- Aseptic conditions are crucial, sterilizing the workspace and equipment to prevent competing microorganisms.
- Plant material (explants) are taken, often from leaves, roots, or buds, due to their active mitosis.
- Explants are sterilized using bleach or alcohol.
- Explants are placed on agar containing nutrients like glucose (for respiration) and amino acids (for protein synthesis).
- Cell division (mitosis) forms a callus.
- The callus can be subdivided to create more calluses.
- Hormones (auxin or cytokines) can be used to induce root formation or other desired development.
- Plantlets are transitioned to soil or water, eventually to a greenhouse.
Advantages of Micropropagation
- Preserves desirable traits in offspring (like taste, quantity of fruit).
- Faster production than growing from seeds.
- Enables seedless fruit production.
Disadvantages of Micropropagation
- Low genetic diversity.
- Increased susceptibility to disease as clones share the same genetic information.
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