Podcast
Questions and Answers
What is the primary purpose of micropropagation?
What is the primary purpose of micropropagation?
Aseptic conditions are important in tissue culture to prevent the introduction of unwanted microorganisms.
Aseptic conditions are important in tissue culture to prevent the introduction of unwanted microorganisms.
True
What types of plant materials can be used as explants in micropropagation?
What types of plant materials can be used as explants in micropropagation?
Leaves, roots, or buds
The hormone used to induce root development in tissue culture is _____ or cytokinins.
The hormone used to induce root development in tissue culture is _____ or cytokinins.
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Match the following concepts with their descriptions:
Match the following concepts with their descriptions:
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Which of the following is a benefit of micropropagation?
Which of the following is a benefit of micropropagation?
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Cloning through tissue culture can produce seedless fruit.
Cloning through tissue culture can produce seedless fruit.
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What is the role of glucose in the tissue culture medium?
What is the role of glucose in the tissue culture medium?
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Micropropagation can lead to low _____ diversity, making plants more vulnerable to diseases.
Micropropagation can lead to low _____ diversity, making plants more vulnerable to diseases.
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What is usually sterilized before beginning tissue culture?
What is usually sterilized before beginning tissue culture?
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Study Notes
Micropropagation and Tissue Culture
- Micropropagation uses tissue culture to grow plants from cells.
- Aseptic conditions are crucial, sterilizing the workspace and equipment to prevent competing microorganisms.
- Plant material (explants) are taken, often from leaves, roots, or buds, due to their active mitosis.
- Explants are sterilized using bleach or alcohol.
- Explants are placed on agar containing nutrients like glucose (for respiration) and amino acids (for protein synthesis).
- Cell division (mitosis) forms a callus.
- The callus can be subdivided to create more calluses.
- Hormones (auxin or cytokines) can be used to induce root formation or other desired development.
- Plantlets are transitioned to soil or water, eventually to a greenhouse.
Advantages of Micropropagation
- Preserves desirable traits in offspring (like taste, quantity of fruit).
- Faster production than growing from seeds.
- Enables seedless fruit production.
Disadvantages of Micropropagation
- Low genetic diversity.
- Increased susceptibility to disease as clones share the same genetic information.
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Description
Test your knowledge on micropropagation and tissue culture! This quiz covers the essential techniques, advantages, and disadvantages of using tissue culture to grow plants from cells. Dive into the specific methods involved and explore how microorganisms can be controlled in a lab setting.
