Micropipetting Techniques and Best Practices Quiz

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Questions and Answers

Which of the following is a step in properly micropipetting?

  • Releasing the plunger slowly and steadily (correct)
  • Using the same pipette tip for multiple samples
  • Holding the micropipette at an angle
  • Depressing the plunger quickly and forcefully

What is the purpose of pre-wetting the pipette tip before aspirating a sample?

  • To increase the accuracy of the volume measurement
  • To prevent air bubbles from forming in the pipette tip (correct)
  • To ensure the tip is clean and free of contaminants
  • To remove any remaining liquid from the previous sample

Why is it important to use a new pipette tip for each sample?

  • To ensure consistent results
  • To minimize the risk of cross-contamination
  • To prevent contamination between samples (correct)
  • To save time and resources

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