Microorganisms Lecture 1: Cells Overview

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Which of the following is NOT a branch of microbiology?

  • Bacteriology
  • Virology
  • Mycology
  • Botany (correct)

Edward Jenner is known as the father of bacteriology.

False (B)

What is the primary advantage of small cell size in prokaryotic cells?

Higher surface to volume ratio allows for faster nutrient and waste exchange.

The study of fungi is known as __________.

<p>Mycology</p> Signup and view all the answers

Which microorganism is known to have a beneficial effect on human nutrition?

<p>Probiotics (C)</p> Signup and view all the answers

Match the following morphological shapes with their descriptions:

<p>Coccus = Spherical shape Bacillus = Rod shape Spirochete = Long, thin, wavy body Spirillum = Curved or spiral shape</p> Signup and view all the answers

Eukaryotic cells generally have a faster growth rate compared to prokaryotic cells due to their larger size.

<p>False (B)</p> Signup and view all the answers

List one function of the cytoplasmic membrane.

<p>Selective barrier, protein anchor, or energy conservation.</p> Signup and view all the answers

Which of the following are types of microbial cells?

<p>Both A and B (C)</p> Signup and view all the answers

All microorganisms are multicellular organisms.

<p>False (B)</p> Signup and view all the answers

What are the two fundamental types of cells found in microorganisms?

<p>Prokaryotic and Eukaryotic</p> Signup and view all the answers

Cyanobacteria can carry out __________, a process that produces oxygen.

<p>photosynthesis</p> Signup and view all the answers

Match the following terms with their definitions:

<p>Prokaryotic cells = Single-celled organisms without a true nucleus Eukaryotic cells = Multicellular organisms with membrane-bound organelles Cyanobacteria = Bacteria that can carry out photosynthesis Anoxic Earth = Earth's early atmosphere without oxygen</p> Signup and view all the answers

Which of the following is NOT a characteristic shared by prokaryotic and eukaryotic cells?

<p>Presence of a nucleus (A)</p> Signup and view all the answers

Microorganisms can live in complex communities.

<p>True (A)</p> Signup and view all the answers

What was the state of the Earth's atmosphere when microorganisms first appeared?

<p>Anoxic (no oxygen)</p> Signup and view all the answers

What is the primary function of endospores?

<p>Survival in unfavorable conditions (A)</p> Signup and view all the answers

Endospores can only remain dormant for a few days before they must germinate.

<p>False (B)</p> Signup and view all the answers

What three steps are involved in endospore activation and germination?

<p>Activation, germination, and outgrowth</p> Signup and view all the answers

The structure connecting the filament to the flagellum motor is called the ______.

<p>hook</p> Signup and view all the answers

Match the parts of a bacterial flagellum with their functions:

<p>Filament = Main part composed of flagellin Hook = Connects filament to motor Flagellum motor = Generates torque for rotation Stator = Surrounds the rotor</p> Signup and view all the answers

What drives the rotation of archaeal flagella?

<p>Hydrolysis of ATP (A)</p> Signup and view all the answers

The core of an endospore includes ribosomes and a nucleoid.

<p>True (A)</p> Signup and view all the answers

What is the fastest known organism on Earth?

<p>Methanocaldococcus</p> Signup and view all the answers

What component makes up the tail of archaeal cell membranes?

<p>Isoprene (A)</p> Signup and view all the answers

All bacteria have peptidoglycan in their cell walls.

<p>True (A)</p> Signup and view all the answers

What type of cell wall structure is primarily found in Gram positive bacteria?

<p>Multiple layers of peptidoglycan</p> Signup and view all the answers

The toxic component of the Gram negative cell wall is called ______.

<p>lipid A</p> Signup and view all the answers

Match the following cell wall structures with their characteristics:

<p>Gram positive = Thick peptidoglycan layer with teichoic acids Gram negative = Thin peptidoglycan layer with outer membrane Archaea = Variety of cell wall types including polysaccharides and proteins Peptidoglycan = Glycan tetrapeptide structure providing rigidity</p> Signup and view all the answers

Which of the following describes the function of porins in Gram negative bacteria?

<p>Act as channels for solute transport (C)</p> Signup and view all the answers

The cell walls of archaea are typically composed solely of peptidoglycan.

<p>False (B)</p> Signup and view all the answers

What is the basic unit of peptidoglycan?

<p>Glycan tetrapeptide</p> Signup and view all the answers

What is the primary function of the mitochondria in eukaryotic cells?

<p>Respiration (D)</p> Signup and view all the answers

Mitosis is unique only to prokaryotic cells.

<p>False (B)</p> Signup and view all the answers

What is the primary role of histones in eukaryotic cells?

<p>To tightly pack DNA into chromosomes</p> Signup and view all the answers

The process of converting a diploid cell into haploid cells is called ______.

<p>meiosis</p> Signup and view all the answers

Match the following cellular structures with their functions:

<p>Chloroplasts = Photosynthesis Hydrogenosomes = Fermentative metabolism Nucleus = Contains chromosomes Mitochondria = Aerobic respiration</p> Signup and view all the answers

Which component of taxonomy refers to the process of characterizing organisms?

<p>Identification (D)</p> Signup and view all the answers

Binomial nomenclature consists of three names for classifying organisms.

<p>False (B)</p> Signup and view all the answers

Who is credited with founding the science of taxonomy?

<p>Carolus Linnaeus</p> Signup and view all the answers

Which of the following statements correctly describes Chargaff’s Rule?

<p>A pairs with T and G pairs with C (A)</p> Signup and view all the answers

In prokaryotes, DNA replication is unidirectional.

<p>False (B)</p> Signup and view all the answers

What enzyme is primarily responsible for replicating chromosomal DNA in prokaryotes?

<p>DNA polymerase III</p> Signup and view all the answers

In RNA transcription, the chain grows in the ______ direction.

<p>5' to 3'</p> Signup and view all the answers

Match the following DNA replication terms with their definitions:

<p>Leading Strand = Synthesis occurs continuously toward the replication fork Lagging Strand = Synthesis occurs discontinuously away from the replication fork Topoisomerase = Enzyme that prevents over-winding of the DNA Proofreading = Mechanism to detect and correct mismatches in DNA</p> Signup and view all the answers

What is the function of primase in DNA replication?

<p>To provide a primer for DNA polymerase (D)</p> Signup and view all the answers

Intrinsic terminators require additional factors for transcription termination.

<p>False (B)</p> Signup and view all the answers

The nucleic acid backbone consists of alternating ______ and ______ molecules.

<p>sugar, phosphate</p> Signup and view all the answers

Flashcards

Prokaryotic Cell

A single-celled organism, lacking membrane-bound organelles, including bacteria and archaea.

Eukaryotic Cell

A complex cell (often multicellular), with membrane-bound organelles, including everything other than bacteria and archaea (like plants, animals, fungi, and protists).

Microbial Cell

Small living units that interact with environment and other cells, often part of communities.

Cell Membrane

The membrane that separates the cell's internal environment from the outside.

Signup and view all the flashcards

Cytoplasm

The jelly-like substance inside a cell, holding organelles & important materials.

Signup and view all the flashcards

Microbial Diversity

The variety of forms and functions of microorganisms, beyond just bacteria.

Signup and view all the flashcards

Cyanobacteria

Ancient bacteria capable of photosynthesis, playing a key role early in Earth's history.

Signup and view all the flashcards

Anoxygenic Phototrophic Bacteria

Bacteria performing photosynthesis without oxygen production.

Signup and view all the flashcards

Prokaryotic Cell Size & Growth

Small size of prokaryotic cells leads to a high surface-to-volume ratio, enabling faster nutrient uptake, waste removal, and overall growth, compared to larger cells.

Signup and view all the flashcards

Bacterial Cell Morphology

Bacteria come in various shapes, including spherical (coccus), rod-shaped (bacillus), spiral (spirillum), and comma-shaped (vibrio).

Signup and view all the flashcards

Cytoplasmic Membrane Function

The cytoplasmic membrane acts as a selective barrier, controls what enters and leaves the cell, anchors essential proteins, and plays a role in energy production for the cell.

Signup and view all the flashcards

Surface-to-Volume Ratio in Cells

Small cells have a higher surface area to volume ratio, enabling faster nutrient intake and waste removal compared to larger cells.

Signup and view all the flashcards

Branches of Microbiology

Microbiology is categorized into different studies, focusing on specific groups of microorganisms like bacteria, protozoa, fungi, viruses, and photosynthetic eukaryotes.

Signup and view all the flashcards

Microorganism Impact on Humans

Microorganisms are significant in various ways, including causing illness, acting as helpers in agriculture and nutrition, contributing to food production, and being useful in industrial processes.

Signup and view all the flashcards

Cell Membrane Structure

A phospholipid bilayer forms the cell membrane, with a hydrophobic core that repels water. This structure is crucial for protecting the cell and controls what enters and exits the cell.

Signup and view all the flashcards

Importance of Bacteria Shape

Bacteria's shape directly affects its ability to function. Different shapes promote different attributes which further affects the role of the bacteria in their environment.

Signup and view all the flashcards

Hydrophilic Region

The water-attracting portion of a phospholipid molecule, typically found on the outer layer of the cell membrane.

Signup and view all the flashcards

Ester Linkage

The chemical bond that connects glycerol to fatty acids in a phospholipid molecule.

Signup and view all the flashcards

Ether Linkage

The chemical bond that connects glycerol to fatty acids in a phospholipid molecule, specifically in archaeal membranes.

Signup and view all the flashcards

Isoprene

A repeating unit that makes up the tail of archaeal phospholipids.

Signup and view all the flashcards

Osmotic Lysis

The bursting of a cell due to the influx of water caused by differences in osmotic pressure.

Signup and view all the flashcards

Peptidoglycan

A rigid polysaccharide found in the cell walls of most bacteria, providing structural strength.

Signup and view all the flashcards

Teichoic Acid

An acidic molecule found in the cell walls of gram-positive bacteria, attached to peptidoglycan.

Signup and view all the flashcards

Lipoteichoic Acid

An acidic molecule found in the cell walls of gram-positive bacteria, attached to the lipids.

Signup and view all the flashcards

Endospore

A highly resistant, dormant form of a bacterial cell, capable of surviving harsh conditions.

Signup and view all the flashcards

Sporulation

The process by which a vegetative bacterial cell converts into an endospore.

Signup and view all the flashcards

Germination

The process by which an endospore reverts back to a vegetative bacterial cell.

Signup and view all the flashcards

Flagella

Long, thin appendages that rotate, propelling bacteria through liquid environments.

Signup and view all the flashcards

Archaella

Similar to flagella, but found in archaea, rotating for movement.

Signup and view all the flashcards

Filament (flagellum)

The main part of a flagellum, made of multiple flagellin protein molecules.

Signup and view all the flashcards

Hook (flagellum)

Connects the filament to the flagellum motor, allowing for rotation.

Signup and view all the flashcards

Flagellum Motor

The complex machinery that powers the rotation of the flagellum.

Signup and view all the flashcards

Gliding Bacteria

Certain bacteria that move across surfaces without using flagella, requiring direct contact with a solid surface.

Signup and view all the flashcards

Eukaryotic Cell Division

The process of cell replication in eukaryotic organisms, involving mitosis and meiosis.

Signup and view all the flashcards

Nucleus

The central control center of eukaryotic cells, housing the chromosomes and nucleolus.

Signup and view all the flashcards

Nucleolus

A structure within the nucleus where ribosomal RNA (rRNA) is synthesized.

Signup and view all the flashcards

Mitochondria

Organelles in eukaryotic cells responsible for cellular respiration, providing energy for the cell.

Signup and view all the flashcards

Hydrogenosomes

Organelles found in anaerobic eukaryotic microbes, producing energy through fermentation.

Signup and view all the flashcards

Taxonomy

The science of classifying organisms, organizing them into groups based on shared characteristics.

Signup and view all the flashcards

Binomial Nomenclature

A two-part naming system for organisms, using genus and species names, established by Carolus Linnaeus.

Signup and view all the flashcards

What are the base pairs in DNA?

Adenine (A) always pairs with Thymine (T), and Guanine (G) always pairs with Cytosine (C).

Signup and view all the flashcards

What is Chargaff's Rule?

The amount of Adenine (A) in DNA always equals the amount of Thymine (T) or Uracil (U) in RNA, and the amount of Guanine (G) always equals the amount of Cytosine (C).

Signup and view all the flashcards

What is semiconservative replication?

When DNA replicates, each new double helix contains one original strand from the parent molecule and one newly synthesized strand.

Signup and view all the flashcards

What is the purpose of the primer in DNA replication?

The primer is a short RNA sequence that provides a starting point for DNA polymerase III to attach and begin DNA synthesis.

Signup and view all the flashcards

What is the difference between the leading and lagging strand?

The leading strand is synthesized continuously in the 5' to 3' direction, while the lagging strand is synthesized discontinuously in Okazaki fragments.

Signup and view all the flashcards

What is the role of topoisomerase in DNA replication?

Topoisomerase prevents supercoiling of the DNA strand by cutting and rejoining the DNA molecule.

Signup and view all the flashcards

What is proofreading in DNA replication?

DNA polymerase can detect mismatches in base pairing and correct them, ensuring high accuracy in DNA replication.

Signup and view all the flashcards

What are the key steps of transcription?

Transcription involves the process of copying DNA into RNA using RNA polymerase. The process includes initiation, elongation, and termination.

Signup and view all the flashcards

Study Notes

Lecture 1: Microorganisms

  • Microorganisms, also called microbes or germs, are too small to be seen with the naked eye, requiring a microscope. They exhibit diverse forms and functions, including single-celled organisms like bacteria and protozoans and multicellular eukaryotes.
  • Microbial cells interact dynamically with their environment and other cells. Two fundamental types are prokaryotic and eukaryotic cells.
  • Prokaryotic cells, including bacteria and archaea, are simpler than eukaryotic cells, which possess membrane-bound organelles.
  • Prokaryotic cells lack a true nucleus, with genetic material located in a nucleoid region. Eukaryotic cells have a true nucleus.
  • Both prokaryotic and eukaryotic cells share common structures such as cell membranes, cytoplasm, ribosomes, and genetic material (DNA).

Microbial Cells: Prokaryotic vs Eukaryotic

  • Prokaryotic: Single-celled organisms (bacteria, archaea). Simpler structure; lack membrane-bound organelles like a nucleus.
  • Eukaryotic: Multicellular or single-celled organisms. More complex structure; possess membrane-bound organelles.

Differences between Prokaryotic and Eukaryotic Cells

  • Prokaryotes are simpler, lacking membrane-bound organelles.
  • Eukaryotic cells have true nuclei and membrane-bound organelles.
  • Shape and structure differ: prokaryotes have simpler forms, while eukaryotes can be diverse.

Microbial Origins and Microscopy

  • Life on Earth first appeared between 3.8 and 4.8 billion years ago.
  • Early life lacked oxygen.
  • Later evolution of photosynthesizing organisms resulted in the Great Oxygenation Event.
  • Robert Hooke was an early observer and described "cells."
  • Antoni van Leeuwenhoek observed and documented bacteria.
  • Microscopy now uses light or electrons, improving resolution and magnification.

Lecture 2: Cells of Bacteria and Archaea

  • Cell Structure: Prokaryotic (bacteria and archaea) cells contain a cell membrane, often a rigid cell wall, and cytoplasm. External structures like capsules, fimbriae, and pili can also be present.
  • Surface-to-Volume Ratio: Small cells have a higher surface-to-volume ratio, allowing for quicker nutrient uptake and waste removal.
  • Cell Membrane Function: The Cytoplasmic membrane separates the inside of the cell from the environment.
  • It acts as a selective barrier regulating what enters and exits the cell
  • Anchors proteins for various functions, including energy production
  • Microbial Cell Morphology: The cell's shape differs between bacterial and archical cells
  • Bacteria is spherical, rods, spiral or curved.

Bacterial Cell Walls

  • Peptidoglycan: A rigid polymer, component of bacterial cell walls, providing structural support and resistance to osmotic lysis.
  • Differences in Gram-positive and Gram-negative bacteria: The structure of the cell wall, including the presence/absence of an outer membrane and peptidoglycan layers, determines how the cell interacts with dyes (distinguish gram reaction).
  • Gram-positive: Thicker peptidoglycan layer, retaining crystal violet stain in a gram stain.
  • Gram-negative: Thinner peptidoglycan layer, losing the stain and taking up counterstain. The presence of an outer membrane further contributes to their differences in cell wall structure.

Lecture 3: Microbial Taxonomy

  • Taxonomy is the science of classifying organisms.
  • Methods and criteria, including morphology (shape and arrangement), staining, growth characteristics, nutritional requirements, and physiological properties, are used to classify organisms.
  • Binomial nomenclature is the formal system of naming species (genus species).

Lecture 4: Flow of Genetic Information

  • DNA Replication: DNA replicates in a semiconservative manner, producing two identical copies of the original DNA molecule.
  • Transcription: DNA is transcribed to mRNA, a process that results in a molecule complementary to a specific DNA sequence.
  • Translation: mRNA is translated into proteins through the process of assembling amino acids.

Lecture 11: RNA Transcription

  • DNA synthesis involves leading and lagging strands with replication forks in both directions.
  • RNA polymerase plays a key role in transcription, using DNA as a template to synthesize RNA.
  • Transcription termination regions are critical sequences that signal the end of transcription, a crucial step for preventing indefinite RNA chains.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

Related Documents

Notes In MicroPara PDF

More Like This

Use Quizgecko on...
Browser
Browser