Microbial concepts

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Questions and Answers

What type of microorganism is yeast and what does it produce when it utilizes sugar?

Yeast is a fungus that produces carbon dioxide when it utilizes sugar.

List two factors that are important for yeast activity in making bread.

Temperature and oxygen supply are important for yeast activity.

Name a microorganism used in cheese production and its role.

Lactobacillus is used in cheese production for fermentation.

What is the function of Asparagillus in food production?

<p>Asparagillus is used in the production of soy sauce.</p> Signup and view all the answers

Which microorganism is primarily associated with beer and wine production?

<p>Saccharomyces is primarily associated with beer and wine production.</p> Signup and view all the answers

Match the brewing methods with their descriptions:

<p>Batch Brewing = Beer is fermented in separate batches. Continuous Brewing = Beer is produced without interruption. Lautering = Process used in continuous brewing. Cold crash = A step in the continuous brewing process.</p> Signup and view all the answers

Match the steps of continuous brewing with their order:

<p>Mashing = Step 1 Filtration = Step 6 Bottling = Step 8 Cooling = Step 4</p> Signup and view all the answers

Match the types of bacteria with their roles:

<p>Lactobacilli = Feeds on lactic acid. Lactococci = Involved in fermentation. Batch Brewing = Uses limited batches. Continuous Brewing = Allows for constant production.</p> Signup and view all the answers

Match the brewing processes to their sequence in continuous brewing:

<p>Brewing Boiling = Step 3 Primary Fermentation = Step 5 Secondary Fermentation = Step 7 Cooling = Step 4</p> Signup and view all the answers

Match the characteristics with the correct brewing method:

<p>Batch Brewing = Requires time between batches. Continuous Brewing = No breaks in production. Primary Fermentation = Critical for flavor development. Filtration = Prevents unwanted bacteria in beer.</p> Signup and view all the answers

What is the primary product of bacteria feeding on lactose during cheese and yogurt production?

<p>Lactic acid (C)</p> Signup and view all the answers

Which type of bacteria is primarily used in the production of Cheddar cheese?

<p>Lactococci (B)</p> Signup and view all the answers

What type of mold is responsible for producing the blue veins in certain cheeses?

<p>Penicillium glaucum (B)</p> Signup and view all the answers

Which of the following bacteria is used to produce Swiss cheese?

<p>Lactobacillus (D)</p> Signup and view all the answers

What is the role of lactic acid in the cheese-making process?

<p>To thicken the milk (C)</p> Signup and view all the answers

What is the first step in the yogurt production process?

<p>Heating the Milk (A)</p> Signup and view all the answers

Which bacteria are primarily responsible for the fermentation in yogurt production?

<p>Lactobacillus bulgaricus and Streptococcus thermophilus (D)</p> Signup and view all the answers

What temperature is the milk heated to during the yogurt-making process?

<p>85°C (C)</p> Signup and view all the answers

What is the main purpose of adding microorganisms during yogurt production?

<p>To improve the sensory quality and taste (B)</p> Signup and view all the answers

Which of the following statements about 'eye-formers' is true?

<p>They cause the formation of eye-like structures in cheese. (A)</p> Signup and view all the answers

Who is credited with the invention of the microscope?

<p>Anthony van Leeuwenhoek (D)</p> Signup and view all the answers

Which scientist is known for developing the gram stain technique?

<p>Christian Gram (A)</p> Signup and view all the answers

Which of the following contributions to microbiology is associated with cholera?

<p>John Snow (D)</p> Signup and view all the answers

What significant development is Louis Pasteur known for?

<p>Pasteurization (A)</p> Signup and view all the answers

Who discovered that Bacillus anthracis causes anthrax?

<p>Robert Koch (D)</p> Signup and view all the answers

What does it mean when quality control results are described as 'in control'?

<p>Results are within the expected range. (C)</p> Signup and view all the answers

Which of the following is NOT a requirement for the quality control process?

<p>Analyze patient demographic data. (C)</p> Signup and view all the answers

What is the primary focus of quality assurance in a laboratory service?

<p>Ensuring that services meet international standards. (D)</p> Signup and view all the answers

Which of the following best describes the importance of using sterile containers and techniques in quality control?

<p>To ensure accurate identification of isolated organisms. (A)</p> Signup and view all the answers

In the context of quality control, what types of materials can QC products be made from?

<p>Human serum, urine, or spinal fluid. (C)</p> Signup and view all the answers

Which of the following components is NOT included in the standard operating procedure (SOP)?

<p>Sample analysis (C)</p> Signup and view all the answers

Which organization is responsible for collecting and distributing quality control materials?

<p>ATCC American Type Culture Collection (D)</p> Signup and view all the answers

What is the correct formatting for typing the full name of an organism in binomial nomenclature?

<p>The genus is capitalized and species is italicized. (C)</p> Signup and view all the answers

Which statement best describes the characteristics shared by species within the same genus?

<p>They display similar staining patterns, types of infections, and biochemical reactions. (D)</p> Signup and view all the answers

In the context of microorganism classification, which of the following pairs correctly identifies a unicellular microorganism?

<p>Bacteria are unicellular organisms. (A), Viruses are noncellular (B), Protozoa are unicellular (C)</p> Signup and view all the answers

Which component of a eukaryotic cell differentiates it from prokaryotic cells?

<p>Presence of a nucleus. (D)</p> Signup and view all the answers

What naming convention should be followed when mentioning an organism for the first time?

<p>Both the genus and species names must be written out the first time. (A)</p> Signup and view all the answers

What is the primary role of the plasma membrane in a eukaryotic cell?

<p>Regulating transport of materials (A)</p> Signup and view all the answers

Which component of the eukaryotic cell is responsible for controlling reproduction and cellular functions?

<p>Nucleus (B)</p> Signup and view all the answers

What is the function of the cell wall in plant cells?

<p>Strengthening and protecting the cell surface (C)</p> Signup and view all the answers

Which structure in the eukaryotic cell is known as the 'powerhouse'?

<p>Mitochondria (D)</p> Signup and view all the answers

What is the arrangement of microtubules in flagella and cilia?

<p>9 + 2 arrangement (B)</p> Signup and view all the answers

What are the three main parts of a flagellum in prokaryotic cells?

<p>Basal body, hook, and filament (A)</p> Signup and view all the answers

What is the primary function of ribosomes in prokaryotic cells?

<p>Protein synthesis (B)</p> Signup and view all the answers

Which component of the prokaryotic cell helps to maintain its structural integrity?

<p>Cell wall (C)</p> Signup and view all the answers

The plasma membrane of prokaryotic cells differs from that of eukaryotic cells in which way?

<p>It is thinner (B)</p> Signup and view all the answers

What is the role of inclusion bodies in prokaryotic cells?

<p>Serving as reserve deposits (C)</p> Signup and view all the answers

What distinguishes resident flora from transient flora in the body?

<p>Resident flora are normally located in a specific area. (C)</p> Signup and view all the answers

Which of the following roles is NOT performed by normal flora?

<p>Actively cause disease in healthy individuals. (A)</p> Signup and view all the answers

What happens when transient flora competes with resident microbiota?

<p>Resident flora may be displaced temporarily. (D)</p> Signup and view all the answers

Which vitamin is produced by normal flora in the body?

<p>Vitamin K (C)</p> Signup and view all the answers

What is a potential consequence of the normal flora colonizing a new location in the body?

<p>Pathogenicity if they overgrow. (A)</p> Signup and view all the answers

Which type of swab is specifically used for the nasopharyngeal area?

<p>Calcium alginate (C)</p> Signup and view all the answers

What is the purpose of Liquid Stuart Medium in specimen preservation?

<p>For pediatric sampling (A)</p> Signup and view all the answers

Which of the following is NOT a container type used for biochemical analysis?

<p>Vacuum-sealed bags (C)</p> Signup and view all the answers

Which type of swab enhances recovery of both aerobic and anaerobic organisms?

<p>Flocked swabs (B)</p> Signup and view all the answers

Which medium is used for the preservation of pathogenic organisms?

<p>Amines Agar gel (A)</p> Signup and view all the answers

Flashcards

Yeast function

Yeast is a fungus that produces carbon dioxide when consuming sugar.

Yeast bread-making factors

Temperature, oxygen, preventing unwanted microbes, and glucose amount affect yeast bread-making.

Lactobacillus use

Lactobacillus is a microorganism used in making milk products like cheese.

Asparagillus use

Asparagillus is a microorganism used in making soy sauce.

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Saccharomyces use

Saccharomyces is a microorganism used in making beer/wine.

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Batch Brewing

A method where beer is fermented in individual batches, with a pause between each production run.

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Continuous Brewing

A brewing method that produces beer without interruption, moving through multiple steps in a continuous flow.

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Mashing

The first step in brewing where crushed grains are mixed with hot water to extract sugars.

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Lautering

The process of separating the liquid (wort) from the spent grains after mashing.

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Fermentation

The process where yeast consumes sugars in the wort, producing alcohol and carbon dioxide.

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Lactose in Cheese and Yogurt

Lactose, a sugar in milk, is broken down by bacteria into lactic acid, which thickens the milk and creates cheese and yogurt.

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Lactobacillus in Cheese

Lactobacillus is a type of bacteria used to make Swiss cheese. It produces a unique flavor and holes in the cheese.

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Lactococci in Cheese

Lactococci are bacteria used to make cheddar cheese, giving it its distinctive sharp flavor.

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Streptococci in Cheese

Streptococci are bacteria used to make mozzarella cheese, known for its stretchy texture.

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Mold on Cheese

Some cheeses have mold on them, like Penicillium candidum (white mold) and Penicillium glaucum (blue mold).

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Yogurt Microorganisms

Yogurt production uses Lactobacillus bulgaricus and Streptococcus thermophilus bacteria to ferment milk.

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Heating Milk for Yogurt

Milk is heated to 85°C before adding bacteria to kill harmful microorganisms and prepare it for fermentation.

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What are Eye-formers?

Eye-formers are microorganisms that create holes in cheese, caused by lactic acid being converted to carbon dioxide gas.

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Yogurt Adjuncts

Microorganisms added to cheese to improve its smell, taste and appearance.

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Yogurt Fermentation

When bacteria like Lactobacillus bulgaricus and Streptococcus thermophilus break down milk sugar (lactose) into lactic acid, it creates yogurt.

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Microbiology

The study of tiny living things too small to see with the naked eye.

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Who invented pasteurization?

Louis Pasteur invented pasteurization, a process that uses heat to kill harmful microbes in liquids like milk.

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What does the Gram stain do?

The Gram stain helps differentiate bacteria into two main groups: gram-positive and gram-negative.

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What did Robert Koch discover?

Robert Koch discovered that the Bacillus anthracis bacterium causes anthrax disease.

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Who developed a smallpox vaccine?

Edward Jenner is known for developing the first vaccine for smallpox.

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Quality Control (QC)

A process that monitors and evaluates the accuracy of lab tests by comparing patient results to expected ranges.

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QC Materials

Substances used in QC, like serum, urine, or spinal fluid, with known levels of analytes, to compare patient results.

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In Control vs. Out of Control

QC results within the expected range are 'in control,' meaning the tests are accurate. Results outside the range are 'out of control,' indicating an issue.

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Quality Assurance (QA)

A system that ensures the overall quality of lab services, including meeting international standards and providing accurate results efficiently.

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Binomial Nomenclature

A system for naming organisms using two parts: the genus (capitalized) and the species (lowercase).

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Resident Flora

Microorganisms that naturally live on our bodies and generally don't cause harm.

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Transient Flora

Microorganisms that temporarily stay on our bodies, often washed off with hygiene.

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ATCC

A nonprofit organization that collects, stores, and distributes quality control materials used in microbiology.

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Standard Operating Procedure (SOP)

A detailed guide for performing specific tasks consistently, ensuring accuracy and safety in a microbiology lab.

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Genus and Species

The genus and species are the two parts of an organism's scientific name, like Escherichia coli where Escherichia is the genus and coli is the species.

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What is Dear, King, Philip, Came, Over, For, Good Soup?

This phrase helps remember the order of classification levels: Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species.

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Unicellular Microorganisms

Microorganisms made of a single cell, like bacteria, fungi, and protozoa.

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Multicellular Microorganisms

Microorganisms composed of multiple cells, like helminths (worms).

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Flagella & Cilia

Hair-like structures that help cells move. They have a unique 9 + 2 arrangement of microtubules.

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Plasma Membrane

A protective barrier surrounding the cell that controls what enters and leaves.

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Cell Wall (Plants)

A rigid outer layer found in plant cells made of polysaccharides like cellulose. It gives the cell shape and strength.

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Cytoplasm

The jelly-like substance that fills the cell. It's where nutrients and waste are moved around.

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Nucleus

The control center of the cell, containing DNA, which controls all cellular activities.

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What is the function of flagella in prokaryotes?

Flagella are long, whip-like appendages that help some prokaryotes move. They are not present in all prokaryotic cells.

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What is the function of the cell wall in prokaryotes?

The cell wall, made of peptidoglycan, provides structural support and protects the prokaryotic cell from damage.

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What is the function of the plasma membrane in prokaryotes?

The plasma membrane regulates the passage of molecules into and out of the cell, acting like a gatekeeper.

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What is the cytoplasm in prokaryotes?

The cytoplasm is a semi-fluid substance that fills the cell and holds the cell's internal components.

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What is the function of ribosomes in prokaryotes?

Ribosomes are responsible for protein synthesis, which is essential for the cell's function.

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What are the roles of normal flora?

Normal flora have multiple roles:

  1. Resides without harming the body.
  2. May become pathogenic under certain conditions.
  3. Produces essential vitamins.
  4. Prevents colonization by harmful microbes.
  5. Produces substances that inhibit harmful species.
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Why can resident flora become pathogenic?

Resident flora can become pathogenic if they colonize a new location or if they compete with other resident microbiota for resources.

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What is the connection between normal flora and disease?

Normal flora usually live harmlessly, but can become pathogenic if they're able to colonize a new location or out-compete other resident microbiota.

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Specimen Containers

Specialized vessels designed for collecting and storing biological samples for analysis, maintaining their integrity.

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Swabs

Small, sterile instruments used to collect samples from various body sites, such as the nose, throat, or vagina. They come in different materials for different purposes.

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Flocked Swabs

Swabs with fibers that stand upright, enhancing the recovery of both aerobic and anaerobic bacteria from the sample.

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Liquid Stuart Medium

A special liquid designed to preserve the integrity of samples collected from children.

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Amines Agar Gel

A gel used to preserve and grow pathogenic organisms, allowing for their identification and study.

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Lag Phase

The initial phase of bacterial growth where bacteria adapt to their new environment and don't reproduce significantly.

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Log Phase

The phase of exponential bacterial growth where the number of bacteria increases rapidly due to favorable conditions.

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Stationary Phase

The phase where bacterial growth plateaus, and the number of bacteria remains relatively constant. This happens when resources become limited or conditions become unfavorable.

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Decline Phase

The final phase where the number of bacteria decreases as resources are depleted and unfavorable conditions prevail.

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What are the four phases of bacterial growth?

The four phases of bacterial growth are Lag, Log, Stationary, and Decline. Each phase represents a different stage in the bacterial population's change over time.

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pH

The measure of acidity or alkalinity of a solution. Microorganisms have optimal pH ranges for growth.

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Osmotic Pressure

The pressure exerted by a solution to prevent the inward flow of water across a semipermeable membrane. High osmotic pressure can inhibit microbial growth.

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Oxygen Requirements

The amount of oxygen a microorganism needs to survive. Some need oxygen (aerobes), some don't (anaerobes), and some tolerate both (facultative anaerobes).

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Temperature and Growth

Microorganisms have optimal temperature ranges for growth. Too cold or too hot can slow down or stop growth.

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Moisture Content

The amount of water available for microbial growth. Most microorganisms need water to survive.

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