Microorganisms in Aquaculture

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Questions and Answers

What role do microbes play in terrestrial and aquatic ecosystems?

  • They play a more important role in sustainable applications than in climate change applications.
  • They are responsible for about half of global primary production and play a role in nutrient cycling. (correct)
  • They are only important in climate change.
  • They have a limited impact regarding the cycling of nutrients.

Which aquaculture method involves fertilizing man-made enclosures to produce blooms of microalgae?

  • Pigment Production
  • Direct Feeding
  • Indirect Feeding
  • Green Water Approaches (correct)

What is a key consideration when using yeast as a dietary component for aquatic species?

  • Yeast has a consistent protein quality across batches.
  • Yeast quality can vary from batch to batch. (correct)
  • Yeast palatability is a challenge with all species of fish.
  • Yeast is only palatable to marine species.

Which method can achieve reduced costs through automation?

<p>Continuous Culture (A)</p> Signup and view all the answers

What key factor has driven research into genetic manipulation of microalgae?

<p>The interest in exploiting microalgae for biofuel production. (D)</p> Signup and view all the answers

What is a common method for introducing DNA into microalgal cells, according to the text?

<p>Electroporation, using electric current to permeabilize the cell membrane. (C)</p> Signup and view all the answers

According to Gressel (2013), how can contamination from competing algae be prevented in open ponds?

<p>Engineering the desired strain with genes encoding herbicide resistance (A)</p> Signup and view all the answers

What is the role of Arthrospira (Spirulina) in aquaculture?

<p>A vitamin and protein supplement (A)</p> Signup and view all the answers

Based on the information, what is one of the main focuses of current probiotics research and development?

<p>Investigating the in vivo proliferation characteristics of probiotics (B)</p> Signup and view all the answers

How do bacteria play a role in recirculating aquaculture systems?

<p>They play an important role in filtration systems. (D)</p> Signup and view all the answers

The text mentions intensive Artemia cyst production started in the 1970s in which location?

<p>Brazil (C)</p> Signup and view all the answers

What limits the use of copepods in aquaculture?

<p>The difficulty in mass-producing them for live feed (A)</p> Signup and view all the answers

Why is it important to correctly identify microalgae?

<p>To ensure reproducibility and reliability in their application in aquaculture (A)</p> Signup and view all the answers

What is the most preferable method for long-term storage of microalgae, according to this document?

<p>Cryopreservation (A)</p> Signup and view all the answers

What is a challenge associated with bacterial isolates described as having probiotic properties?

<p>Maintenance by individual research groups, potentially affecting long-term security (A)</p> Signup and view all the answers

What is the most highly produced microalgae, by tonnage, in China?

<p><em>Arthrospira</em> spp. (D)</p> Signup and view all the answers

Why is maintenance of stock cultures essential for microalgal strains used industrially?

<p>To check cultures before using them as starters for new cultures. (C)</p> Signup and view all the answers

Which factor significantly contributes to fluctuations in Artemia harvests from the Great Salt Lake?

<p>Changing natural hydrological and climatic conditions (D)</p> Signup and view all the answers

Which of these factors is described as a key driver in expanding the use of microorganisms in aquaculture?

<p>Decreased aquaculture production costs when using microbes (A)</p> Signup and view all the answers

How does the text suggest aquaculture companies could benefit in the long-term?

<p>By investing more heavily in research in microbiology (C)</p> Signup and view all the answers

According to the document, what skills are required for someone working in aquaculture microbiology?

<p>Skills in microbiology, molecular approaches, and knowledge of aquaculture (C)</p> Signup and view all the answers

In relation to the culture medium for rotifers, which factor presents a particular challenge?

<p>Maintaining the health of such cultures (C)</p> Signup and view all the answers

To what factor are both egg ratio and swimming velocity related, in rotifer culture systems?

<p>The maintenance of rotifer cultures early warning on detoriation (C)</p> Signup and view all the answers

What advance has already made it possible to perform more procedures on the genome of microalgae?

<p>Sequencing of multiple genomes (D)</p> Signup and view all the answers

Genetic technologies are not yet well developed for which species?

<p>Artemia (B)</p> Signup and view all the answers

Which parameter related to the cultivation of Arthrospira species is of particular importance?

<p>The use of an appropriate strain of species (A)</p> Signup and view all the answers

What advance increased Artemia harvesting throughout Asia in the 1980s?

<p>Transplantation (B)</p> Signup and view all the answers

Aside from direct feeding and creating pigments, what third common purpose exists for using microalgae?

<p>For indirect feeding (D)</p> Signup and view all the answers

What are a couple of advantages that rotifers have over other species?

<p>Smaller size and fast generation times (D)</p> Signup and view all the answers

What type of aquatic setting do Artemia typically inhabit in nature?

<p>Hypersaline environments like salt lakes (C)</p> Signup and view all the answers

Which factor of note allows microalgae to be commercially grown even in the absence of light?

<p>Heterotrophic capabilities (D)</p> Signup and view all the answers

Which compound of interest has been used to enrich rotifers and Artemia?

<p>DHA (C)</p> Signup and view all the answers

What is a potential benefit of growing biomass in wastewater?

<p>Removing nutrients from the wastewater (A)</p> Signup and view all the answers

What is the purpose of applying lipid emulsions of different specific algae to rotifers?

<p>To achieve a suitable fatty acid content for the fish species to be fed (A)</p> Signup and view all the answers

What is the effect of engineering microalgae to produce avermectin?

<p>Resistance to zooplankton contamination (D)</p> Signup and view all the answers

Flashcards

Aquaculture

Farming aquatic organisms, microbes to finfish.

Role of Microbes

Microbes cycle nutrients in terrestrial, aquatic ecosystems.

Pseudo-Green Water

Algae added into fish larval rearing tanks.

Green Water Benefits

Enhance nutritional value by using green water.

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Direct Feeding

Direct microalgae feeding.

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Heterotrophic Microalgae

Heterotrophs can grow using industrial fermenters.

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Genetic Technologies

Genetic tech to understand the systems biology.

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Genetic Modification

Improve microalgae. OptimiZe quality for aquaculture feeds.

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Engineered Traits

Beneficial genetically engineered characteristics.

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Arthrospira

Photosynthetic cyanobacteria. Used as food.

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Probiotic Bacteria

Reduce pathogens effects. Used in China and America.

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Bacteria role

Removes waste and recycles in aquaculture systems.

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Artemia

Aquaculture feed of small crustaceans.

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Rotifers

Live feed of larval marine fish stages.

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Thraustochytrids

Provide long-chain polyunsaturated acid.

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Chlorella vulgaris

Commonly used for microalgae to feed rotifers.

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Haematococcus pluvialis

Microalgae source of astaxanthin.

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Study Notes

Genetic Resources for Microorganisms in Aquaculture

  • Aquaculture involves farming aquatic organisms from microbes to shellfish and finfish.
  • Aquaculture's increasing production has led to substantial growth in fish production for human consumption.
  • Aquaculture contributed more than wild-caught fisheries for the first time in 2014.
  • In 2014, global aquaculture production accounted for 44.1% of total global fish production.
  • To feed the growing population, fish consumption (especially from aquaculture) plays an important role.
  • Fish consumption is key to meeting the nutritional needs of a growing population.

Microbes' Role

  • Microbes play a crucial role in cycling nutrients in terrestrial and aquatic ecosystems.
  • Marine microbes are responsible for about half of global primary production.
  • Microbes in natural marine and freshwater ecosystems are key components of food webs and nutrient cycling.
  • They are used as live feeds, probiotics, and filtration systems in aquaculture.
  • Microbes are indispensable resources for the growth of shellfish and finfish in aquatic ecosystems.
  • Microbes can substitute for fishmeal and fish oil, which is growing more expensive.
  • Meeting the nutritional needs of aquaculture species includes microbes.
  • 30.8% of world fish production by aquaculture consists of non-fed species, notably carp, and bivalve molluscs like clams, oysters and mussels.
  • Genomic approaches now let you rapidly determine the composition of natural microbial communities.

Study Overview

  • This will provide information about the genetic resources of key microorganisms on which aquaculture depends.
  • These microorganisms such as microalgae enhance the nutritional quality of Artemia and rotifers.
  • Bacteria are used for human diet supplements and include species that improve fish and shellfish survival and growth.
  • Bacteria also play an important role in filtration systems needed in recirculating aquaculture systems.
  • Zooplankton (Artemia spp. and rotifers) is widely used as feed for the aquaculture industry.
  • China is the world's biggest aquaculture producer making up over 80 percent of world aquaculture production with other countries including Indonesia, India, Viet Nam and the Philippines.

Microorganisms in Aquaculture

  • Microalgae uses are divided into green water approaches, direct feeding, indirect feeding, and pigment production.
  • Green water is water in which microalgae grow naturally, or are added.
  • Green water is an important sector in aquaculture because of the freshwater fish aquaculture that it supports.
  • The fish and shrimp are raised without additional aquaculture feed.
  • Microalgae used for green water production are Nannochloropsis spp., Chlorella vulgaris, Isochrysis spp. and Tetraselmis spp.
  • Microalgae species such as Nannochloropsis spp., Pavlova spp., Isochrysis spp., Tetraselmis spp., Thalassiosira weissflogii, Dunaliella spp. and Chaetoceros spp. are commercially used.
  • Thraustochytrids are marine microorganisms considered as microalgae that are rich sources of long-chain polyunsaturated fatty acids
    • This has potential as feed additives in aquaculture. The genus Schizochytrium is the most commonly used in aquaculture.

Bacteria in Aquaculture

  • They are increasingly used to reduce pathogen effects being extensively used in China and South America.
  • Probiotic application improves disease resistance or growth of aquaculture species.
  • Many species of Gram-negative bacteria and Gram- positive bacteria are considered for use in aquaculture.
  • Their modes of action includes competitive exclusion or immunostimulation as well as improvement in appetite or feed conversion that leads to better growth

Zooplankton in Aquaculture

  • It is used as live feed.
  • The Artemia spp. comprises both zygogenetic and parthenogenetic groups.
  • It is found in the Americas being the most important
  • Several strains of Artemia are used for aquaculture
  • They are a resource for selection of characteristics desirable in aquaculture ( in particular nutritional value)
  • Rotifers are used in aquaculture, with the Brachionus being the most important genus and other genus used by farmers in Asia

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