Microorganism Staining Techniques Quiz
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Questions and Answers

What is the primary purpose of using Acidfast stain in microbiology?

  • To visualize flagella on bacterial cells.
  • To differentiate between Gram-positive and Gram-negative bacteria.
  • To identify the presence of spores in bacteria.
  • To stain bacteria with a high lipid content in their cell wall. (correct)

    • Why is Ziehl-Neelsen stain known as the 'hot method'?

  • It uses a heat-based primary stain.
  • It utilizes a heat gun to dry the stained slide quickly.
  • It requires steam bathing the prepared smear after addition of the primary dye. (correct)
  • It involves placing the stained slide under a hot lamp for visualization.

    • What color do acid-fast organisms appear as when stained using the Kinyoun stain?

  • Yellow
  • Blue
  • Green
  • Red (correct)

    • Which of the following special stains is used to visualize capsule or slime layer in bacteria?

    <p>Hiss stain</p> Signup and view all the answers

    What is the purpose of using LAMB (Loeffler Alkaline Methylene Blue) stain in microbiology?

    <p>To visualize metachromatic granules in bacterial cells.</p> Signup and view all the answers

    Which staining method does not involve heat after adding the primary stain?

    <p>Kinyoun stain</p> Signup and view all the answers

    What is the primary purpose of staining procedures in microbiology?

    <p>To facilitate visualization of microorganisms under a microscope</p> Signup and view all the answers

    Which of the following statements about simple stains is true?

    <p>They can be either water-based or alcohol-based</p> Signup and view all the answers

    What is the primary mechanism by which simple stains adhere to bacterial cells?

    <p>Electrostatic attraction between positively charged dyes and negatively charged bacterial cells</p> Signup and view all the answers

    Which type of differential staining procedure distinguishes between Gram-positive and Gram-negative bacteria?

    <p>Gram stain</p> Signup and view all the answers

    According to the information provided, which of the following bacterial genera is an exception to the general rule for Gram staining of cocci?

    <p>Neisseria</p> Signup and view all the answers

    Which of the following bacterial genera is an exception to the general rule for Gram staining of bacilli?

    <p>Bacillus</p> Signup and view all the answers

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