Microorganism Quantification Methods Quiz

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

How many microscope fields are typically counted in the Petroff-Hausser slide method?

  • 5
  • 12
  • 8
  • 10 (correct)

What is the main disadvantage of using the Petroff-Hausser slide for direct microscopic counting?

  • The slide does not provide accurate measurements of microorganisms.
  • It cannot distinguish between living and dead cells during counting. (correct)
  • The counting area is too small for accurate counting.
  • The slide requires complex staining techniques.

In the Breed method, bacteria are counted in at least how many different areas under the microscope?

  • 8
  • 5
  • 10 (correct)
  • 12

What is the basis for counting in the Thoma slide method?

<p>Counting in a volume of 0.1 mm3 (B)</p> Signup and view all the answers

What is the final result obtained by the counting method with the Thoma slide?

<p>Number of microorganisms per milliliter (C)</p> Signup and view all the answers

Which method is most suitable for determining the amount of long filament bacteria?

<p>Chemical measurement of cell mass (A)</p> Signup and view all the answers

Why are direct microscopic counting methods not suitable for samples containing low concentrations of microorganisms?

<p>Inability to distinguish between live and dead microorganisms (D)</p> Signup and view all the answers

What is the disadvantage of direct microscopic counting methods?

<p>Inability to distinguish between live and dead microorganisms (B)</p> Signup and view all the answers

What must be done prior to counting motile cells using direct microscopic counting methods?

<p>Cell immobilization (A)</p> Signup and view all the answers

Which method is suitable for determining the amount of single-celled organisms such as bacteria and yeasts?

<p>Total cell count (B)</p> Signup and view all the answers

What is the main disadvantage of direct microscopic counting methods for determining the total cell number?

<p>Inability to distinguish between live and dead microorganisms (B)</p> Signup and view all the answers

Which method is most suitable for determining the amount of long filament bacteria?

<p>Measurement of dry weight of cells (D)</p> Signup and view all the answers

What must be done prior to counting motile cells using direct microscopic counting methods?

<p>Add a chemical fixative to immobilize the cells (B)</p> Signup and view all the answers

In the Breed method, bacteria are counted in at least how many different areas under the microscope?

<p>6 different areas (D)</p> Signup and view all the answers

Why are direct microscopic counting methods not suitable for samples containing low concentrations of microorganisms?

<p>They may not accurately distinguish between live and dead microorganisms (A)</p> Signup and view all the answers

What is the main disadvantage of using the Breed method for direct microscopic counting?

<p>Inability to measure all dead or live cells accurately (C)</p> Signup and view all the answers

In the Thoma slide method, the counting area is divided into how many large squares?

<p>16 large squares (B)</p> Signup and view all the answers

What is the basis for counting in the Petroff-Hausser slide method?

<p>Dividing the middle part of the well area by lines (B)</p> Signup and view all the answers

What is the advantage of using the Thoma slide method for counting blood, sperm, and yeast?

<p>Counting in a volume of 0.1 mm3 (C)</p> Signup and view all the answers

Why are direct microscopic counting methods not as accurate as culture methods in measuring all dead or live cells?

<p>Difficulty in distinguishing between live and dead cells (B)</p> Signup and view all the answers

Flashcards are hidden until you start studying

More Like This

Microorganism Nutrition Quiz
24 questions
Microorganism Classification
8 questions
Use Quizgecko on...
Browser
Browser