Microbiology: Viruses and Human Microbiome
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Questions and Answers

Which of the following viruses is characterized by a polyhedral capsid and fibers at each corner?

  • Ebola virus
  • HIV
  • T-even bacteriophage
  • Adenovirus (correct)

The T-even bacteriophage has a helical tail.

True (A)

What kind of genetic material do DNA viruses contain?

DNA

The __________ bacteriophage possesses a polyhedral capsid and a helical tail.

<p>T-even</p> Signup and view all the answers

Match the following characteristics with their respective viruses:

<p>Adenovirus = DNA virus with polyhedral capsid T-even bacteriophage = DNA virus with helical tail Ebola virus = RNA virus causing hemorrhagic fever HIV = RNA virus that affects the immune system</p> Signup and view all the answers

What is a common feature of RNA viruses?

<p>They all have capsids. (C)</p> Signup and view all the answers

Fibers are common structural components found in all types of viruses.

<p>False (B)</p> Signup and view all the answers

What structure is commonly found at each corner of the adenovirus?

<p>fiber</p> Signup and view all the answers

What is the first step in the viral replication cycle?

<p>Attachment (A)</p> Signup and view all the answers

The lytic cycle involves the integration of viral DNA into the bacterial DNA.

<p>False (B)</p> Signup and view all the answers

What occurs during the integration step of the lysogenic cycle?

<p>Viral DNA is integrated into bacterial DNA.</p> Signup and view all the answers

New viruses leave the host cell during the ______ stage.

<p>release</p> Signup and view all the answers

Match the following terms with their definitions:

<p>Lytic Cycle = Viral replication that results in cell destruction Lysogenic Cycle = Viral DNA integrates into host DNA Penetration = Viral DNA enters the bacterial cell Release = New viruses exit the host cell</p> Signup and view all the answers

Which step follows penetration in the viral replication cycle?

<p>Integration (A)</p> Signup and view all the answers

In the lytic cycle, new viruses are produced and released at the same stage.

<p>True (A)</p> Signup and view all the answers

Name a key difference between the lytic and lysogenic cycles.

<p>The lytic cycle destroys the host cell, while the lysogenic cycle integrates viral DNA into the host's DNA.</p> Signup and view all the answers

What is the first step in the lytic cycle of a phage?

<p>Attachment (A)</p> Signup and view all the answers

The viral DNA enters the host cell before the attachment of the phage.

<p>False (B)</p> Signup and view all the answers

What structure of the phage is responsible for binding to the host cell?

<p>Capsid</p> Signup and view all the answers

The phage injects its genome into the ______ of the host cell.

<p>cytoplasm</p> Signup and view all the answers

Match the following stages of the lytic cycle with their descriptions:

<p>Attachment = Phage binds to a specific host cell Penetration = Viral DNA enters the host's cytoplasm Biosynthesis = Synthesis of new viral components Release = New viruses are released from the host cell</p> Signup and view all the answers

Which of the following describes the role of host-specific match in phage attachment?

<p>It ensures that phages only infect the correct bacterial hosts. (B)</p> Signup and view all the answers

Biosynthesis is the stage where new viral components are produced.

<p>True (A)</p> Signup and view all the answers

What happens during the penetration step of the lytic cycle?

<p>The viral DNA is injected into the host cell.</p> Signup and view all the answers

What enzyme do retroviruses like HIV use to convert RNA into DNA?

<p>Reverse transcriptase (C)</p> Signup and view all the answers

Retroviruses are released from the host cell by lysis.

<p>False (B)</p> Signup and view all the answers

In the process of reverse transcription, what does RNA get converted into?

<p>cDNA</p> Signup and view all the answers

The process by which viral components are synthesized in a host cell is called __________.

<p>biosynthesis</p> Signup and view all the answers

Match the following viral processes with their definitions:

<p>Entry = Viruses attach to host cell receptors Uncoating = Release of viral nucleic acid into host cell Biosynthesis = Assembly of viral components Maturation = Formation of the complete viral particle</p> Signup and view all the answers

What happens to cDNA integrated into the host DNA?

<p>It remains inactive for years. (B)</p> Signup and view all the answers

Budding is the method by which most viruses are released from host cells.

<p>True (A)</p> Signup and view all the answers

During the __________ phase of viral reproduction, remaining viral components assemble to form new virus particles.

<p>maturation</p> Signup and view all the answers

Which of the following statements about antibiotics is correct?

<p>Antibiotics can inhibit cell wall biosynthesis in bacteria. (A)</p> Signup and view all the answers

Archaea contain peptidoglycan in their cell walls.

<p>False (B)</p> Signup and view all the answers

What is a major characteristic of methanogens?

<p>They produce methane from hydrogen gas and carbon dioxide.</p> Signup and view all the answers

Archaea are now thought to be more closely related to __________ than to bacteria.

<p>eukarya</p> Signup and view all the answers

Match the antibiotic with its function:

<p>Penicillin = Inhibits cell wall biosynthesis Erythromycin = Inhibits protein biosynthesis Tetracycline = Affects protein synthesis Fluroquinolone = Inhibits DNA replication</p> Signup and view all the answers

What type of organism obtains energy by oxidizing inorganic chemicals?

<p>Chemoautotroph (D)</p> Signup and view all the answers

Mutualism refers to a relationship where one population benefits at the expense of another.

<p>False (B)</p> Signup and view all the answers

Which type of bacteria benefit from living in the human intestines by releasing vitamins K and B12?

<p>Mutualistic bacteria</p> Signup and view all the answers

Bacteria such as __________ and __________ form resistant endospores under unfavorable conditions.

<p>Bacillus, Clostridium</p> Signup and view all the answers

Match the following relationships with their definitions:

<p>Mutualism = Both species benefit Commensalism = One benefits, other is not affected Parasitism = One benefits at the expense of the other</p> Signup and view all the answers

Which of the following is true about parasitic bacteria?

<p>They benefit at the expense of the host. (C)</p> Signup and view all the answers

Endospores can be destroyed by ultraviolet radiation.

<p>False (B)</p> Signup and view all the answers

What type of bacteria primarily depend on organic molecules for both carbon and energy?

<p>Chemoheterotrophs</p> Signup and view all the answers

Flashcards

DNA Virus

A virus whose genetic material is deoxyribonucleic acid (DNA).

Adenovirus

A DNA virus with a polyhedral capsid (a many-sided structure) and a fiber at each corner.

T-even bacteriophage

A DNA virus with a polyhedral head and a helical tail containing DNA.

Polyhedral capsid

A many-sided structure for a virus.

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Helical tail

A spiral-shaped tail structure in some viruses.

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Virus structure

The overall organization and arrangement of parts in a virus.

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RNA Virus

A virus containing ribonucleic acid (RNA) as genetic material.

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Phage attachment

Phages attach to specific host cells based on matching receptors on the phage and host cell.

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Host cell receptors

Specific molecules on the surface of a host cell that phages attach to.

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Phage Capsid

Protein coat surrounding the phage's genetic material.

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Bacterial Cell Wall

Protective outer layer that surrounds the bacterial cell.

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Penetration (Phage)

Phage injects its DNA into the host cell during the lytic cycle.

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Viral Attachment

Phage binds to bacterial cell surface receptors.

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Viral Penetration

Viral DNA enters the host bacterium's cell.

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Viral Replication

Production of new viral components (DNA, proteins, etc).

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Lytic Cycle

Viral replication destroys the host cell.

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Lysogenic Cycle

Viral DNA integrates into host bacterial DNA.

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Viral Release

New viruses leave host, usually killing it.

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Viral Maturation

Viral components assemble to form complete viruses.

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Viral Integration

Viral DNA combines with bacteria's DNA.

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Viral Biosynthesis

The assembly of viral components (like protein coats and genetic material) inside a host cell

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Retrovirus Replication

Retroviruses use reverse transcriptase to convert their RNA into DNA, which then gets incorporated into the host cell's DNA.

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Reverse Transcriptase

An enzyme that retroviruses use to convert their RNA into DNA.

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Viral Maturation

The final stage of viral replication, where the assembled virus particles take on a complete form.

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Viral Release

The process by which newly assembled viruses leave the host cell.

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Retrovirus Latency

Retrovirus can remain in the host genome as a silent infection for years.

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Chemoautotroph

Organisms that obtain energy by oxidizing inorganic chemicals and use carbon dioxide as their carbon source.

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Photoheterotroph

Organisms that obtain energy from sunlight and get carbon from other organic molecules.

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Chemoheterotroph

Organisms that get energy and carbon from organic molecules.

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Commensalism

A symbiotic relationship where one organism benefits, and the other is neither helped nor harmed.

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Mutualism

A symbiotic relationship in which both organisms benefit.

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Parasitism

A symbiotic relationship where one organism benefits (parasite) at the expense of another (host).

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Endospore

A dormant, resistant structure formed by some bacteria in unfavorable conditions.

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Endospore of Clostridium tetani

A resistant, dormant structure formed by some bacteria, like Clostridium tetani, to survive harsh conditions.

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Antibiotics

Chemical compounds that inhibit or kill bacteria, but not necessarily other living things.

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Protein Biosynthesis Inhibition

Antibiotics like Erythromycin, Tetracycline interrupt bacterial protein production.

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Cell Wall Biosynthesis Inhibition

Penicillin class of antibiotics stop bacterial cell wall construction.

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Bacterial Resistance

The ability of bacteria to withstand the effects of antibiotics, increasing over time.

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Archaea

A domain of single-celled microorganisms distinct from bacteria and eukaryotes.

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Peptidoglycan

A polymer found in bacterial cell walls, absent in archaea cells.

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Methanogens

A type of Archaea that produces methane.

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Study Notes

Viruses

  • Viruses are very small, approximately 20-300nm.
  • Characterized by: size and shape (helical, polyhedral, complex); presence or absence of outer envelope; type of nucleic acid (DNA/RNA).
  • Viruses have at least two parts: covering (capsid, envelope) and inner core (nucleic acid).
  • Some viruses have an outer envelope surrounding the capsid.
  • The capsid is an outer protein coat made of protein subunits called capsomers.
  • The nucleic acid core contains DNA or RNA.
  • Viruses can only reproduce within a host cell.

The Human Microbiome

  • The human microbiome is the community of microorganisms in a specific environment
  • A recent study from the American Academy of Microbiology estimated each human ecosystem contains roughly 100 trillion microbial cells and just 37 trillion human cells.

Course Outline

  • The course will cover Viruses, The Prokaryotes, The Bacteria, and The Archaea.

Learning Outcomes

  • Explain the basic structure and life cycle of a virus.
  • Explain the basic structure and characteristics of bacteria and archaea.
  • Relate the importance of microorganisms in life.

Lesson 01: Viruses

  • Viruses are obligate intracellular parasites.
  • They can only reproduce within host cells.
  • Viruses are incapable of replicating their own genetic material.
  • Once inside a living cell, they hijack the cell's protein synthesis machinery to replicate nucleic acids, capsids, and viral enzymes.

Lesson 02: The Prokaryotes

  • Prokaryotes range in length from 1 to 5 µm.
  • They are mainly unicellular.
  • They lack a membrane-bound nucleus and other membrane-bound organelles.
  • Most prokaryotes have a cell wall, primarily composed of peptidoglycan.
  • Some prokaryotes produce a capsule or slime layer surrounding the cell wall.
  • The capsule helps protect the bacteria from phagocytosis and enables attachment to surfaces.

Lesson 03: Bacteria

  • Bacteria are often identified using Gram staining.
  • Gram-positive bacteria retain the dye, appearing purple.
  • Gram-negative bacteria lose the dye, appearing pink.
  • The difference in staining is due to the cell wall structure.
  • Bacteria can be categorized by their shape (cocci, bacilli, spirilla).

Lesson 04: Archaea

  • Archaea were previously classified as bacteria.
  • They lack peptidoglycan in their cell walls.
  • Their cell walls have a different structure.
  • Archaea are biochemically more similar to eukaryotes than to bacteria.
  • Many archaea live in harsh conditions (extremophiles).
  • One example group are methanogens which produce methane.

Prokaryotic Reproduction

  • Prokaryotes reproduce asexually through binary fission.
  • Genetic material can be exchanged through transduction, conjugation, or transformation.

Bacterial Oxygen Requirements

  • Bacteria have different oxygen requirements.
  • Obligate aerobes require oxygen.
  • Obligate anaerobes cannot tolerate oxygen.
  • Facultative anaerobes can grow in the presence or absence of oxygen.

Modes of Nutrition

  • Autotrophs make their own food.
  • Photoautotrophs use sunlight.
  • Chemoautotrophs use inorganic chemicals.
  • Heterotrophs get energy from other organisms.
  • Photoheterotrophs obtain energy from sunlight but need organic molecules for carbon.
  • Chemoheterotrophs obtain carbon and energy from organic compounds.

Prokaryote Relationships

  • Prokaryotes interact with other organisms in various ways, including commensalism (one species benefits, the other is unaffected), mutualism (both benefit), and parasitism (one benefits at the expense of the other).

Bacteriophages: Lytic and Lysogenic Cycles

  • Bacteriophages infect bacteria.
  • In the lytic cycle, the virus replicates and causes the host cell to burst, releasing new viruses.
  • In the lysogenic cycle, the viral DNA integrates into the host cell's DNA. This viral DNA is now called a prophage.
  • The lysogenic cycle can stay dormant, or the prophage will restart the cycle and enter the lytic phase.

Retroviruses

  • Retroviruses contain reverse transcriptase.
  • Reverse transcriptase converts viral RNA to DNA.
  • The viral DNA then integrates into the host cell's DNA, forming a provirus.
  • Retroviruses replicate when the host DNA replicates.

Antibiotics

  • Antibiotics can inhibit protein synthesis or cell wall biosynthesis.
  • Bacterial resistance to antibiotics is a significant concern.

Summary of Virus Subtopics

  • Viruses have a covering (capsid or an additional envelope) and an inner core (nucleic acid).
  • Viruses are obligate intracellular parasites, host-specific, and differ significantly in their structure
  • Viruses can have lytic or lysogenic cycles.
  • Retroviruses utilize reverse transcription (RNA to DNA) during reproduction.

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This quiz explores the fundamental aspects of viruses, including their structure, classification, and life cycle. Additionally, it covers the human microbiome and its role in the ecosystem. Enhance your understanding of microbial life and its implications for human health.

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