Questions and Answers
Define chemically defined and complex media. Give an example of each type of media.
Chemically defined media is composed of exact amounts of chemically pure, specifically identified organic or inorganic components. Example: glucose salt broth. Complex media is composed of organic materials that are not chemically pure and include unspecified chemical components. Example: Nutrient Broth.
What is enriched media and give an example? Why is it necessary for growing fastidious bacteria?
Enriched media is general purpose media with additional components to support fastidious bacteria. Example: Blood Agar. It is necessary because fastidious bacteria require specific nutrients to grow.
What is the relationship between the amount of bacteria in a culture and the absorbance?
Directly related. As the amount of bacteria increases, turbidity increases, which raises absorbance.
Would heterotrophic organisms grow well in inorganic salt media? Why or why not?
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Why is complex media generally used to cultivate microorganisms?
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Study Notes
Media Types
- Chemically Defined Media: Composed of exact quantities of pure organic/inorganic components. Examples include glucose salt broth and inorganic synthetic broth.
- Complex Media: Contains organic materials that are not chemically pure or specifically identified. Examples include Nutrient Broth/Agar, Tryptic Soy Broth/Agar, and Blood Agar.
Enriched Media
- Enriched media is general-purpose media with additional supplements for the growth of fastidious bacteria, which require specific nutrients.
- Examples include Blood Agar and Nutrient Broth with Yeast Extract.
Relationship Between Bacterial Amount and Absorbance
- The amount of bacteria in a culture is directly related to absorbance; increased bacterial growth leads to higher turbidity and absorbance readings.
- Transmittance is indirectly related; 100% transmittance indicates no bacterial presence (0 cells).
Heterotrophic Organisms and Inorganic Salt Media
- Heterotrophic organisms need organic compounds for energy and carbon sources.
- Growth is inhibited in inorganic salt media due to the lack of organic molecules necessary for energy production.
Use of Complex Media
- Complex media provides a wide range of nutrients such as amino acids, sugars, vitamins, and minerals essential for the growth of most microorganisms.
- Most microbes thrive in complex media due to its rich nutrient content.
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Description
Dive into the world of microbiology with this Unit 6 flashcard quiz. Learn the differences between chemically defined and complex media along with examples for each type. Perfect for students aiming to enhance their understanding of media composition in microbiological studies.
