Microbiology: Transformation and Conjugation

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Questions and Answers

What is the primary mechanism identified in Griffiths' experiment that demonstrates transformation in bacteria?

  • Uptake of DNA from the environment (correct)
  • Direct cell fusion between bacteria
  • Viral infection of the bacterial cells
  • Conjugation between two bacterial cells

Which type of transduction results in the transfer of specific bacterial genes through a bacteriophage?

  • Abortive transduction
  • Specialized transduction (correct)
  • Sexduction
  • Generalized transduction

In the context of conjugation, what advantage does an Hfr strain have over an F+ strain?

  • Increased metabolic capabilities
  • Ability to transfer genomic DNA (correct)
  • Resistance to antibiotics
  • Higher replication rate of plasmids

What is the primary function of R plasmids in bacteria?

<p>Provide resistance to antibiotics (A)</p> Signup and view all the answers

Which type of mutation results in a change of a single nucleotide and can affect a single amino acid in a protein?

<p>Missense mutation (A)</p> Signup and view all the answers

What is a key characteristic of insertion sequences in prokaryotic transposable elements?

<p>They have inverted repeat sequences at both ends (A)</p> Signup and view all the answers

Which of the following best describes the Ames test?

<p>An assay to assess mutagenicity of compounds (A)</p> Signup and view all the answers

What function do Ti plasmids serve in plant cells?

<p>Inducing tumors in plants (D)</p> Signup and view all the answers

Which of the following best defines the concept of 'cured' in the context of plasmids?

<p>Loss of plasmid DNA from the bacterial cell (A)</p> Signup and view all the answers

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Flashcards

Transformation

Process where bacteria take up genetic material from their environment, enabling them to acquire new traits. It was discovered by Griffith's experiment with Streptococcus pneumoniae.

Competence

The ability of a bacterium to take up exogenous DNA and incorporate it into its own genome. This ability can be influenced by factors like the bacterial strain, the growth phase, and the presence of certain chemicals.

Transduction

A transfer of genetic material from one bacterium to another via bacteriophages (viruses that infect bacteria). Discovered by Zinder and Lederberg.

Generalized Transduction

Occurs when a bacteriophage packages random pieces of bacterial DNA and delivers them to a new host cell. This can lead to the transfer of any gene of the original bacterial host.

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Specialized Transduction

A specific type of transduction where a bacteriophage integrates its DNA into the host's genome, carrying bacterial DNA with it during the replication cycle

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Conjugation

The transfer of genetic material from one bacterium to another through direct contact. This requires cell-to-cell contact and the presence of a special DNA element called the F-factor.

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Plasmid

A type of DNA molecule found in bacteria that can replicate independently of the host chromosome. It can carry genes that provide bacteria with advantages such as antibiotic resistance.

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Mutation

A permanent change in the DNA sequence of an organism. These changes can occur spontaneously or be induced by mutagens.

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Transposable Elements

Mobile genetic elements that can jump around within a genome. They can insert themselves into different locations, influencing the expression of nearby genes.

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Study Notes

Transformation, Transduction, and Conjugation

  • Zero lecture, discovery of transformation, Griffiths experiment, mechanism of transformation, factors affecting transformation
  • Competence- development of competence in S. pneumoniae, transduction discovery, Lederberg and Tatum's experiment, mechanism and types of transduction (generalized, specialized, sexduction, abortive)

Conjugation

  • Conjugation discovery, experimental evidence, F-factor (F+ & Hfr), mechanism of conjugation, cross between Hfr, F+, and F-, conjugants and application, features of T4 genetics, genetic basis of lytic versus lysogenic switch of phage lambda

Plasmids

  • Types of plasmids (F plasmid, R plasmids, colicinogenic plasmids, Ti plasmids), linear plasmids, yeast micron plasmid, plasmid replication and partitioning, host range, plasmid incompatibility, plasmid amplification, regulation of copy number, curing of plasmids

Mutations and Mutagenesis

  • Mutations and mutagenesis, definition and types of mutations, physical and chemical mutagens, uses of mutations, reversion and suppression, true revertants, intra- and inter-genic suppression, Ames test, mutator genes

Transposable Elements

  • Prokaryotic transposable elements (insertion sequences, composite transposons, non-composite transposons), replicative transposition, non-replicative transposition, eukaryotic transposable elements (Yeast Ty retrotransposon, Drosophila P elements), uses of transposons, uses of transposition, revision

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