Microbiology Study Notes
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Questions and Answers

Which two-component system is essential for Salmonella's survival within macrophages due to low magnesium levels?

  • PhoP/PhoQ (correct)
  • EnvZ/PmrA
  • PmrA/PmrB
  • OmpR/EnvZ
  • In the context of bacterial adaptation, what is the role of tetraphosphate guanosine during starvation?

  • It acts as a sensor kinase in two-component systems.
  • It directly activates virulence genes.
  • It functions as an 'alarmone' signaling starvation. (correct)
  • It promotes the transition to the exponential growth phase.
  • Which two-component system is crucial for both Salmonella infection and Shigella flexneri virulence?

  • PhoQ/PmrA
  • PhoP/PhoQ
  • OmpR/EnvZ (correct)
  • PmrA/PmrB
  • Which of the following correctly describes the sequence of events during bacterial interaction with host cells, as shown in the diagram?

    <p>Adhesion -&gt; Phagocytosis -&gt; Phagolysosome formation -&gt; Degradation -&gt; Exocytosis (A)</p> Signup and view all the answers

    During intracellular infection, what is the immediate precursor to the phagolysosome?

    <p>The phagosome (A)</p> Signup and view all the answers

    According to the stringent response model, what triggers the production of the alarmone during starvation?

    <p>The presence of uncharged tRNA (A)</p> Signup and view all the answers

    In Legionella pneumophila, which phenotypic switch is associated with the 'transmissive' state?

    <p>The post-exponential phase (B)</p> Signup and view all the answers

    What is the role of the sensor kinase in the two-component regulatory systems described?

    <p>To receive a signal and activate a response regulator (C)</p> Signup and view all the answers

    What is the effect of a Salmonella Typhimurium phoP or phoQ null mutation on virulence in mice?

    <p>Highly attenuated for virulence (C)</p> Signup and view all the answers

    Under which condition are PhoP-repressed genes (prg) induced?

    <p>High extracellular magnesium concentrations (C)</p> Signup and view all the answers

    Which of the following is NOT a known consequence of Quorum Sensing in bacteria?

    <p>Cellular respiration (D)</p> Signup and view all the answers

    What is the primary function of the PhoP/PhoQ two-component system (TCS)?

    <p>Controlling lipid A modifications and gene expression in response to magnesium concentration (D)</p> Signup and view all the answers

    Which of the following is an example of a PhoP-activated gene product involved in lipid A modification?

    <p>L-4-aminoarabinose (C)</p> Signup and view all the answers

    What is the role of autoinducers in quorum sensing?

    <p>They accumulate proportionally to cell density and signal gene expression changes. (A)</p> Signup and view all the answers

    What is the effect of low magnesium concentration on PhoP-activated genes (pag)?

    <p>Increased expression (C)</p> Signup and view all the answers

    What modification to lipid A does the gene pagP encode?

    <p>Addition of a palmitate (C)</p> Signup and view all the answers

    Which secretion system in Gram-negative bacteria is known for pathogen-host protein transport and is homologous to the flagellar apparatus?

    <p>Type III (A)</p> Signup and view all the answers

    Which of the following secretion systems is characteristic of autotransporter functionality?

    <p>Type V (B)</p> Signup and view all the answers

    What is the distinctive feature of Type IV secretion systems in Gram-negative bacteria?

    <p>DNA transport related to conjugation (A)</p> Signup and view all the answers

    Which secretion system is specifically related to the transport mechanism similar to bacteriophages?

    <p>Type VI (D)</p> Signup and view all the answers

    In which Gram-negative bacteria secretion system is the ESX-1 to ESX-5 associated with vaccine candidates?

    <p>Type VII (D)</p> Signup and view all the answers

    Which of the following is an autoinducer molecule found in Gram-negative bacteria?

    <p>N-acyl-homoserine lactone (AHL) (C)</p> Signup and view all the answers

    What is the role of the luxS gene in bacterial communication?

    <p>Facilitates inter-species communication (D)</p> Signup and view all the answers

    What type of signaling molecules do Bacillus spp. produce to interfere with quorum sensing?

    <p>AHL-lactonase (AiiA) (B)</p> Signup and view all the answers

    In the context of quorum sensing, what is the primary function of the lux operon?

    <p>To synthesize bioluminescent proteins (A)</p> Signup and view all the answers

    Which of the following processes is NOT regulated by quorum sensing?

    <p>Nucleotide synthesis (C)</p> Signup and view all the answers

    Which organism is a key example of symbiosis involving bioluminescence?

    <p>Vibrio fisheri (D)</p> Signup and view all the answers

    What biological function does bioluminescence provide in symbiotic relationships?

    <p>Attraction of prey (D)</p> Signup and view all the answers

    Which compound can act as an antipathogenic agent through quorum sensing inhibition?

    <p>Halogenated furanones (A)</p> Signup and view all the answers

    What is the primary focus of quorum sensing?

    <p>Bacterial cell density (B)</p> Signup and view all the answers

    Which type of communication is facilitated by the AI-2 autoinducer?

    <p>Inter-species communication (A)</p> Signup and view all the answers

    Which component is NOT associated with Gram-negative bacteria in quorum sensing?

    <p>Peptide autoinducer (AIP) (A)</p> Signup and view all the answers

    What type of system utilizes oligopeptide autoinducers?

    <p>Quorum sensing in Gram-positive bacteria (C)</p> Signup and view all the answers

    Which is NOT a characteristic of the LuxR/LuxI system?

    <p>Active transport of autoinducers (C)</p> Signup and view all the answers

    What role does phospho-LuxO play in Vibrio harveyi?

    <p>Facilitating toxin coregulated pili expression (B)</p> Signup and view all the answers

    Which of the following accurately describes diffusion sensing?

    <p>Concentration of signaling molecule is key (A)</p> Signup and view all the answers

    Which organism is known to use the LuxI system for quorum sensing?

    <p>Vibrio harveyi (A)</p> Signup and view all the answers

    What is one benefit of using Desia pulchra brominated furanones in relation to biofilms?

    <p>Inhibition of AHL signal generation (A)</p> Signup and view all the answers

    How do treated Pseudomonas aeruginosa biofilms with synthetic furanone C30 appear?

    <p>Flat and undifferentiated, less tolerant to tobramycin (D)</p> Signup and view all the answers

    What factor contributes to drug resistance and persistence in biofilms?

    <p>Periplasmic glucans and physical barriers (D)</p> Signup and view all the answers

    Which of the following describes phenotypic heterogeneity in biofilms?

    <p>Co-existence of different traits in a clonal population (D)</p> Signup and view all the answers

    What role does 'bet-hedging' play in mixed-species biofilms?

    <p>Facilitates survival through expression of different traits (D)</p> Signup and view all the answers

    Which factor is NOT mentioned as a cue that influences phenotypic heterogeneity?

    <p>Genetic mutation (C)</p> Signup and view all the answers

    What is the significance of physiological heterogeneity within biofilms?

    <p>It allows different sectors to withstand varied environmental stress (A)</p> Signup and view all the answers

    What is a potential consequence of the signal reception inhibition in biofilms?

    <p>Reduced ability of bacteria to form biofilms (A)</p> Signup and view all the answers

    Flashcards

    Desia pulchra

    A bacterium known for producing brominated furanones that disrupt biofilm formation.

    Brominated furanones

    Compounds that inhibit bacterial communication and biofilm development without selecting for resistance.

    Quorum sensing

    A process where bacteria communicate to coordinate behavior based on population density.

    Biofilm structure disruption

    The breaking down of biofilm, making bacteria more susceptible to antibiotics.

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    Phenotypic heterogeneity

    Variation in bacterial traits within a population, allowing adaptation to changing environments.

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    Division of labor in bacteria

    Different bacterial traits coexist to enhance survival in varying conditions.

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    Physical barriers (EPS)

    Extracellular polysaccharides that create a protective layer in biofilms.

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    AHL signal molecules

    Chemical signals used in quorum sensing by some bacteria to coordinate community behavior.

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    OmpR/EnvZ system

    A two-component regulatory system important for Salmonella infection.

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    PhoP/PhoQ system

    A regulatory system needed for Salmonella to survive in low magnesium environments.

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    Stringent response model

    A response to nutrient deficiency that involves a phenotypic switch.

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    Phagocytosis

    Process by which bacteria are engulfed by immune cells.

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    Phagosome

    A vesicle formed around a bacterium during phagocytosis.

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    Phagolysosome

    The fusion of a phagosome with lysosomes for bacterial degradation.

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    Exocytosis

    Process of releasing bacterial fragments after degradation.

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    Nutrient availability

    Regulation of bacterial behavior based on available nutrients.

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    Autoinducers

    Signaling molecules used in quorum sensing to signal bacterial population density.

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    Autoinducer-2 (AI-2)

    A signaling molecule identified in many bacteria, important for inter-species communication.

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    LuxS gene

    A gene involved in the production of AI-2 for quorum sensing in bacteria.

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    Bioluminescence

    The production of light by living organisms, often seen in marine species like Vibrio fisheri.

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    Lux operon

    A set of genes that control bioluminescence in Vibrio species.

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    Quorum sensing inhibitors

    Substances that interfere with quorum sensing to prevent bacterial communication and virulence.

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    Biofilms

    Structured communities of bacteria that can form on surfaces, often involving quorum sensing.

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    PhoP/PhoQ TCS

    A two-component system regulating gene expression in Salmonella based on Mg2+ levels.

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    PhoP-activated genes (pag)

    Genes induced under low Mg2+ conditions, promoting virulence in Salmonella.

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    PhoP-repressed genes (prg)

    Genes induced under high Mg2+ conditions, reducing virulence in Salmonella.

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    Avirulent mutants

    Strains lacking phoP or phoQ that show reduced virulence in hosts.

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    Covalent lipid A modifications

    Chemical changes to lipid A that occur in response to the PhoP/PhoQ system.

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    Quorum sensing processes

    Gene expression activities regulated by quorum sensing including biofilm formation and virulence.

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    Diffusion Sensing

    A method where bacteria detect the concentration of signaling molecules (autoinducers) in the environment regardless of their cell density.

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    AHL (Acylated Homoserine Lactone)

    A type of autoinducer used primarily in Gram-negative bacteria for intra-species communication.

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    AI-2 (Autoinducer 2)

    An autoinducer involved in inter-species communication found in more than 55 bacterial species.

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    LuxR/LuxI System

    A common quorum sensing system in Gram-negative bacteria involving LuxI for autoinducer production and LuxR as an activator.

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    Two-Component System

    A regulatory mechanism in bacteria involving a receptor (sensor) and a response regulator, often used in quorum sensing pathways.

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    Phospho-LuxO

    A protein that binds to RpoN to activate the expression of repressors in quorum sensing in Vibrio species.

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    Type I Secretion System

    A protein secretion system in Gram-negative bacteria using haemolysin transporter.

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    Type II Secretion System

    A general secretory pathway used by Gram-negative bacteria for protein transport.

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    Type III Secretion System

    A system in Gram-negative bacteria that transports proteins using a flagellar homolog and targets host cells.

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    Type IV Secretion System

    A secretion system homologous to conjugation systems for protein or DNA transport in pathogens.

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    Type VI Secretion System

    A unique system in some bacteria that uses components similar to bacteriophages for interbacterial transport.

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    Study Notes

    Microbiology Study Notes

    • Microbiology is the study of microorganisms, including bacteria, viruses, fungi, and protozoa.
    • A key topic is the regulation of bacterial virulence which is influenced by environmental factors (e.g., temperature, pH, osmolarity), host cells (e.g., contact, intracellular conditions), and bacterial density (quorum sensing) and phenotypic heterogeneity and persistence, and inter-kingdom signaling.
    • The central dogma of molecular biology explains how genetic information flows from DNA to RNA to protein, which is vital for understanding regulation. The process involves replication (DNA polymerase), transcription (RNA polymerase), and translation (ribosome).
    • Transcriptional regulators such as (RegA, RegB) bind to operator sequences near promoters, as activators or repressors. They are often dimers with helix-turn-helix (HTH) DNA-binding motif.
    • Sigma factors bind to different promoters, crucial for regulation, specifically: Sigma-70, Sigma-32, Sigma-54, and Sigma-38.
    • Regulation of bacterial virulence is affected by temperature, as in Shigella flexneri and Listeria monocytogenes, through transcriptional and translational regulation, respectively.
    • Two-component systems (TCS) are key in regulating bacterial virulence in response to environmental signals like pH, ions, and osmolarity. The systems involve a sensor kinase and a response regulator.
    • Bacterial transport encompasses different mechanisms like porins (outer membrane) for small molecules, transporters (plasma membrane) for specific substrates, and siderophores for iron uptake.
    • Bacterial secretion systems (type I-VII) are diverse export machinery which are used to transport proteins and toxins out of the cell.
    • Quorum sensing is a mechanism in which bacteria coordinate their behaviors by signaling molecules known as autoinducers. This control gene expression and synchronizes behaviours.
    • Specific autoinducers such as AHLs (acyl homoserine lactones) are common to intraspecies communication and interspecies interactions (ex. Al-2).
    • Biofilms are complex communities of bacteria associated with surfaces, leading to drug resistance; drug tolerance often differs with persistence.
    • Inter-kingdom signaling, a phenomenon where bacteria communicate and respond with other organisms, such as eukaryotes, also plays a vital role in pathogenesis. L. pneumophila uses LAI-1 for this.
    • Host-controlled signalling compounds like adrenaline and Noradrenaline influence pathogen behaviour.
    • Bioluminescence (e.g., in Vibrio fisheri) is a process regulated by quorum sensing, which is linked to symbiotic relationship.

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    Description

    Explore the fundamentals of microbiology including the regulation of bacterial virulence and the central dogma of molecular biology. This quiz covers key concepts such as transcriptional regulation, sigma factors, and environmental influences on microorganisms. Perfect for students seeking to deepen their understanding of microbial processes.

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