Microbiology Quiz: Staphylococcus and Clostridia

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Questions and Answers

What is the Gram staining result for Staphylococcus aureus?

  • Variable
  • Gram positive (correct)
  • Not determinable
  • Gram negative

Which of the following is a characteristic of Clostridia?

  • Large, blunt-ended rods (correct)
  • Cocci-shaped
  • Non-motile
  • Obligate aerobes

Which species is NOT an important member of the Clostridia group?

  • Escherichia coli (correct)
  • Clostridium botulinum
  • Clostridium difficile
  • Clostridium tetani

What is the motility characteristic of Clostridia?

<p>Motile (C)</p> Signup and view all the answers

What is the oxygen requirement for Clostridia?

<p>Obligate anaerobes (C)</p> Signup and view all the answers

What does the coagulase test result indicate for Staphylococcus aureus?

<p>Coagulase positive (B)</p> Signup and view all the answers

What is a major pathogenic factor of Clostridium perfringens?

<p>Secretes 12 exotoxins (B)</p> Signup and view all the answers

Which condition can Clostridium spores withstand for some time?

<p>Boiling temperatures (A)</p> Signup and view all the answers

What is a primary mechanism by which Staphylococcus aureus attaches to host cells?

<p>It utilizes fibronectin-binding proteins. (C)</p> Signup and view all the answers

Which type of skin infection does NOT typically require antibiotic therapy?

<p>Folliculitis (D)</p> Signup and view all the answers

What major injury does the alpha-toxin from Staphylococcus aureus cause?

<p>It leads to cell destruction by leaking cytosol. (C)</p> Signup and view all the answers

What complication can arise from carbuncles caused by Staphylococcus aureus?

<p>Systemic bacteremia. (A)</p> Signup and view all the answers

What condition is characterized by localized, superficial, spreading crusty skin lesions, particularly in children?

<p>Impetigo (A)</p> Signup and view all the answers

Which factor contributes to Staphylococcus aureus's resistance to phagocytosis?

<p>Fibrinogen-binding clumping factor. (D)</p> Signup and view all the answers

What is the clinical significance of furuncles?

<p>Forms around foreign bodies. (B)</p> Signup and view all the answers

Toxinosis refers to disease caused by:

<p>Toxins in the absence of infection. (D)</p> Signup and view all the answers

Which of the following pathogens is categorized as a Gram (+) rod?

<p>Clostridium perfringens (B)</p> Signup and view all the answers

Which of the following viruses is primarily associated with skin infections?

<p>Herpesviruses (B)</p> Signup and view all the answers

What is the primary pathogenic characteristic of anaerobic organisms?

<p>They thrive in oxygen-depleted environments. (A)</p> Signup and view all the answers

Which of the following antimicrobial agents is generally effective against Gram (-) bacteria?

<p>Gentamicin (B)</p> Signup and view all the answers

In the context of skin and wound infections, which pathogenic organism is most commonly associated with necrotizing fasciitis?

<p>Streptococcus pyogenes (A)</p> Signup and view all the answers

Which exotoxin of Clostridium perfringens is most crucial for its virulence in tissues?

<p>α toxin (D)</p> Signup and view all the answers

What is a primary effect of α toxin produced by Clostridium perfringens?

<p>Lysis of erythrocytes (D)</p> Signup and view all the answers

Which of the following clinical conditions is NOT associated with Clostridium perfringens?

<p>Pneumonia (B)</p> Signup and view all the answers

What is the role of Clostridium perfringens enterotoxin in infection?

<p>Disrupts ion transport in the intestine (C)</p> Signup and view all the answers

Which characteristic best describes the clinical significance of myonecrosis caused by Clostridium perfringens?

<p>Leads to the rapid spread of necrosis (C)</p> Signup and view all the answers

How does Clostridium perfringens utilize its hydrolytic enzymes during infection?

<p>To promote tissue liquefaction and spread (C)</p> Signup and view all the answers

Which biochemical action is NOT performed by the α toxin of Clostridium perfringens?

<p>Inhibition of fluid loss (B)</p> Signup and view all the answers

What is the role of the degradative enzymes produced by Clostridium perfringens?

<p>Facilitate bacterial metabolism (A)</p> Signup and view all the answers

What is the drug of choice for treating infections caused by nasopharyngeal colonization?

<p>Penicillin (A)</p> Signup and view all the answers

Which of the following describes the laboratory identification of Pasteurella multocida?

<p>Culture on blood agar shows small, translucent non-hemolytic colonies (D)</p> Signup and view all the answers

Which characteristic is NOT associated with Propionibacterium acnes?

<p>Aerobic oxygen requirement (A)</p> Signup and view all the answers

What is the primary nutrient source for Propionibacterium acnes?

<p>Fatty acids from sebum (D)</p> Signup and view all the answers

What laboratory method can be used to identify Propionibacterium acnes?

<p>Quantitative real-time polymerase chain reaction (A)</p> Signup and view all the answers

How does Propionibacterium acnes contribute to acne formation?

<p>It blocks the exit of sebum (A)</p> Signup and view all the answers

Which statement regarding the treatment of acne caused by Propionibacterium acnes is accurate?

<p>Antimicrobial drugs are more effective than topical agents (C)</p> Signup and view all the answers

What is a common clinical outcome of active bacterial growth in Propionibacterium acnes infections?

<p>Formation of pus (B)</p> Signup and view all the answers

What symptoms developed in the postal worker after handling the suspicious envelope?

<p>Cutaneous boils with necrosis (C)</p> Signup and view all the answers

What was the notable laboratory finding related to the postal worker's condition?

<p>Elevated WBC count (A)</p> Signup and view all the answers

What severe respiratory symptom did the postal worker develop?

<p>Severe dyspnea (C)</p> Signup and view all the answers

What was observed in the chest X-ray of the postal worker?

<p>Widened mediastinum (A)</p> Signup and view all the answers

What was the infectious agent causing the conditions in the postal worker and his wife?

<p>Bacillus anthracis (C)</p> Signup and view all the answers

What effect did the illness have on the livestock on the postal worker's farm?

<p>Several animals also died (D)</p> Signup and view all the answers

What characteristic of the rash in the 80-year-old man indicates a specific viral infection?

<p>Vesicular on one side (A)</p> Signup and view all the answers

What type of cells were observed in the smear from the vesicle in the elderly man?

<p>Multinucleated giant cells (D)</p> Signup and view all the answers

Flashcards

Gram-positive cocci

A group of bacteria characterized by their thick cell wall, retaining crystal violet dye in a Gram stain test, and spherical shape.

Skin infections

Infections affecting the skin, often caused by bacteria, viruses, or fungi.

Pathogenic microorganisms

Microbes capable of causing disease

Bacterial infections

Infections caused by bacteria, like staphylococcus or streptococci.

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Viral infections

Infections caused by viruses, such as herpes or measles, affecting skin or tissues

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Staphylococcus aureus infection

A bacterial infection caused by Staphylococcus aureus bacteria, leading to localized skin infections like abscesses.

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Abscess

A localized collection of pus in the skin or tissues.

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Folliculitis

An infection around a hair follicle.

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Furuncle

A boil; a subcutaneous abscess around a foreign body.

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Carbuncle

A large, deep, multi-loculated skin infection.

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Impetigo

Superficial, spreading skin lesion with crusts, common in children.

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Bacterial infection pathogenesis

The steps by which a bacteria causes disease, including attachment, cell damage, and resistance to immune responses.

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Toxinosis

Disease caused by bacterial toxins without bacterial infection.

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Staphylococcus aureus

A Gram-positive bacterium, often found on the skin, identified by its grape-like clusters and positive coagulase test.

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Coagulase test

A test used to identify Staphylococcus aureus; a positive result indicates the bacterium's ability to clot plasma.

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Clostridia

A group of bacteria characterized by large, blunt-ended rod shapes, spore production, and obligate anaerobe characteristics.

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Clostridium perfringens

A type of Clostridia often found in the gut and soil; it produces several exotoxins and is a pathogen.

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Obligate anaerobes

Microorganisms that require the absence of oxygen to survive.

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Spore resistance

Spores of Clostridia can survive harsh conditions like chemical disinfectants and even boiling.

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Gram-positive

Describes bacteria that retain the crystal violet stain in a Gram stain procedure.

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Catalase test

A test used to determine if a bacteria produces the enzyme catalase.

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Alpha toxin (Clostridium perfringens)

The most important toxin in Clostridium perfringens, acting as a phospholipase C that damages cell membranes by causing lysis.

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Theta toxin (Clostridium perfringens)

A cholesterol-dependent hemolysin and a virulence factor in Clostridium perfringens, important factor for bacteria to cause damage.

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Enterotoxin (Clostridium perfringens)

A small, heat-sensitive protein produced by Clostridium perfringens that disrupts ion transport in the intestines causing loss of fluid and intracellular proteins resulting in food poisoning.

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Degradative enzymes (Clostridium perfringens)

Enzymes produced by Clostridium perfringens that break down tissue, promoting the spread of infection and causing tissue liquefaction.

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Myonecrosis (gas gangrene)

A serious infection caused by Clostridium perfringens, where bacteria damage muscles resulting in gas buildup and tissue death.

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Anaerobic cellulitis

A soft tissue infection that happens in the absence of oxygen.

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Food Poisoning (Clostridium perfringens)

A common illness caused by Clostridium perfringens, caused by enterotoxin and its effects on cells, resulting in fluid loss and symptoms related to poisoning.

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Pasteurella multocida

A bacterium often found in animals, especially cats and dogs. It can cause infections in humans, primarily through bites or scratches.

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Pasteurella multocida: Culture

Grows on blood agar plates, forming small, translucent colonies with no red blood cell destruction (non-hemolytic).

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Pasteurella multocida: Microscopy

When viewed under a microscope, the bacteria appear stained at both ends, with the middle remaining unstained.

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Propionibacterium acnes: Characteristics

These bacteria are rod-shaped or branched, don't move (non-motile), are gram-positive, thrive in the absence of oxygen (anaerobe), produce hydrogen peroxide (positive catalase test), and can be cultured on blood agar.

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Propionibacterium acnes: Habitat

This bacterium resides in the sebaceous glands of the skin, which produce oil (sebum).

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Propionibacterium acnes: Acne

This bacterium is a major contributor to acne, a common skin condition, especially in adolescents.

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Propionibacterium acnes: Medical Device Infections

In addition to causing acne, this bacteria can also infect patients with medical devices, such as catheters.

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Propionibacterium acnes: Acne Mechanism

The bacteria feed on sebum, causing a buildup of oil. The sebaceous gland continues to produce sebum, leading to more bacteria growth. This cycle results in excess bacteria and inflammation, causing acne lesions.

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Cutaneous Boils

Painful, pus-filled bumps on the skin, often caused by Staphylococcus aureus. They usually have a central core of dead tissue called an eschar.

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Anthrax

A serious infectious disease caused by the bacteria Bacillus anthracis. It can affect the skin, lungs, or digestive system.

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Mediastinum

The space in the chest between the lungs, containing the heart, trachea, esophagus, and major blood vessels.

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Pleural Effusions

An abnormal buildup of fluid in the space between the lungs and the chest wall (pleural space).

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Leukemia

A type of cancer that affects the blood-forming cells in the bone marrow, often leading to a weakened immune system.

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Vesicular Rash

A rash characterized by small, fluid-filled blisters.

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Multinucleated Giant Cells

Cells with multiple nuclei, often seen in viral infections. They can be found in the skin lesions of certain viral diseases.

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Intranuclear Inclusions

Structures found inside the nuclei of cells, often associated with viral infections.

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Study Notes

Microbiological Basis of Disease

  • The course covers the microbiological basis of disease, encompassing various systems.

Clinically Important Bacteria

  • A pie chart categorizes clinically important bacteria into groups:
    • Gram-positive cocci
    • Gram-positive rods
    • Gram-negative cocci
    • Gram-negative rods
    • Spirochetes
    • Anaerobic organisms
    • Other (chlamydia, mycoplasma)

Clinically Important Viruses

  • The presentation categorizes viruses based on their structure, including:
    • Single stranded, nonenveloped
    • Double stranded, nonenveloped
    • Double stranded, enveloped
    • Single stranded, positive strand, icosahedral, nonenveloped
    • Single stranded, positive strand, icosahedral or helical, enveloped
    • Single stranded, negative strand, helical, enveloped

Clinically Important Fungi

  • The table details various types of fungal infections categorized by their location:
    • Cutaneous (Superficial mycoses, Tinea, Piedra, Candidiasis)
    • Subcutaneous (Chromoblastomycosis, Sporotrichosis, Mycetoma, Phaeohyphomycosis)
    • Opportunistic (Aspergillosis, Candidosis, Cryptococcosis, Geotrichosis, Mucormycosis, Fusariosis, Trichosporonosis, Others*)
    • Systemic (Aspergillosis, Blastomycosis, Candidiasis, Coccidioidomycosis, Histoplasmosis, Cryptococcosis, Paracoccidioidomycosis, Geotrichosis)

Clinically Important Parasites

  • Protozoa:
    • Intestinal (Entamoeba histolytica, Giardia lamblia, Cryptosporidium parvum, Balantidium coli)
    • Urogenital (Trichomonas vaginalis)
    • Blood and tissues (Plasmodium species, Toxoplasma gondii, Trypanosoma species, Leishmania species, Naegleria fowleri, Acanthamoeba castellanii, Balamuthia mandrillaris, Babesia microti)
  • Helminths:
    • Cestodes (tapeworms)
    • Trematodes (flukes)
    • Nematodes (roundworms)

Systems Overview

  • The diagram shows the major body systems (nervous, respiratory, muscular, circulatory, digestive, skeletal and integumentary).

Learning Outcomes

  • List pathogenic microorganisms, characteristics, classifications, methods of identification, pathogenicity factors, and mechanisms.
  • Recognize clinical pictures of common pathogens, their etiology, and basic management.
  • State antimicrobial agents for microbial pathogens and list preventive measures including vaccines.

Chapter 9: Skin and Wound Infections

  • Outline: the presentation groups pathogens by type (Bacteria, Viruses, Fungi) which specifically impact skin and wound infection.

Bacterial Species Details

  • Details are provided for specific bacterial species, including Bacillus anthracis, Streptococci, Staphylococcus aureus, Clostridium perfringens, Pasteurella multocida, and Propionibacterium acnes. These details include morphology, staining, oxygen requirements, and other key characteristics.

Viral Species Details

  • Details are provided for specific viral species including Herpesviruses, Rubella virus, Parvoviruses and how they are classified.

Fungal Species Details

  • Specific fungi relevant to skin and wound infections are included.

Additional Details and Case Studies

  • Case studies are presented, with clinical histories, laboratory findings, and discussions on possible infectious agents.
  • Further details, such as properties, pathogenesis, clinical significance, and laboratory identification, are provided for a number of bacteria, fungi, and viruses relevant to the topic of the presentation.

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