Microbiology Quiz on Pathogenic Bacteria
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Questions and Answers

What specimen is used for diagnosing Neisseria meningitidis carriers?

A nasopharyngeal swab (West swab) is used.

What are the culture conditions required for Moraxella catarrhalis?

Moraxella catarrhalis should be cultured at 37°C in 5-10% CO2 conditions.

Describe the morphological characteristics of Corynebacterium diphtheriae.

Corynebacterium diphtheriae are Gram-positive bacilli arranged in Chinese letters.

What biochemical reaction is used to identify Moraxella catarrhalis?

<p>Moraxella catarrhalis does not produce acid from glucose, maltose, lactose, and sucrose.</p> Signup and view all the answers

How would you describe the colonies of Moraxella catarrhalis on culture media?

<p>The colonies are dry, greyish-white, opaque, and 'scoot' when pushed with a loop.</p> Signup and view all the answers

What is the primary virulence factor of Corynebacterium diphtheriae?

<p>The primary virulence factor is the production of exotoxin.</p> Signup and view all the answers

What type of agar is best for culturing Corynebacterium diphtheriae?

<p>Corynebacterium diphtheriae grows best on Loeffler's serum agar.</p> Signup and view all the answers

Why is Neisseria meningitidis often mistaken for commensal Neisseria species?

<p>Both can appear similar in initial examinations, particularly in carrier states.</p> Signup and view all the answers

What are the primary modes of transmission for diphtheria?

<p>Diphtheria is primarily transmitted through oral droplets and physical contact.</p> Signup and view all the answers

What are the key symptoms associated with diphtheria?

<p>Key symptoms include sore throat, low-grade fever, and the presence of a pseudomembrane on the tonsils.</p> Signup and view all the answers

How is diphtheria diagnosed both clinically and in a laboratory setting?

<p>Clinically, diphtheria is diagnosed based on symptoms, while laboratory confirmation involves throat or nasal swabs and virulence tests.</p> Signup and view all the answers

What are some diseases caused by diphtheroids?

<p>Diphtheroids can cause urinary tract infections, bacteraemia, and osteomyelitis.</p> Signup and view all the answers

What role does cell-mediated immunity play in tuberculosis?

<p>Cell-mediated immunity is crucial for controlling Mycobacterium tuberculosis infections.</p> Signup and view all the answers

Why can't Mycobacterium tuberculosis be stained with simple stains?

<p>Mycobacterium tuberculosis has a waxy cell wall rich in lipids and mycolic acids, making it impermeable to most stains.</p> Signup and view all the answers

What diagnostic tests are used for tuberculosis?

<p>The tuberculin test and Quanti-Feron test are used for diagnosing tuberculosis.</p> Signup and view all the answers

What is the etiological agent of pulmonary tuberculosis in humans?

<p>The etiological agent of pulmonary tuberculosis in humans is Mycobacterium tuberculosis.</p> Signup and view all the answers

What is the primary purpose of the Ziehl-Neelsen stain?

<p>To differentiate between acid-fast bacilli and non-acid-fast bacilli.</p> Signup and view all the answers

What components are present in the Lowenstein-Jensen medium?

<p>Malachite green dye and egg enrichment.</p> Signup and view all the answers

How does the BACTEC culture system assist in the rapid diagnosis of tuberculosis?

<p>It detects radioactive CO2 released when Mycobacteria utilize palmitic acid.</p> Signup and view all the answers

Describe the appearance of Bacillus cereus under Gram stain.

<p>Gram positive, non-capsulated, motile, spore-forming bacilli arranged in chains.</p> Signup and view all the answers

What types of specimens are often collected for the laboratory identification of anthrax?

<p>Sputum, skin exudates, and blood.</p> Signup and view all the answers

What is the significance of the polychrome methylene blue stain in identifying anthrax?

<p>It demonstrates the polypeptide capsule of Bacillus anthracis.</p> Signup and view all the answers

What type of staining technique is employed in the identification of acid-fast bacilli?

<p>Differential staining technique.</p> Signup and view all the answers

List one pathogenic and one saprophytic species from the Bacillus group.

<p>Pathogenic: Bacillus anthracis; Saprophytic: Bacillus cereus.</p> Signup and view all the answers

What distinguishes obligate aerobes from facultative anaerobes?

<p>Obligate aerobes can only grow in the presence of free O2, while facultative anaerobes can grow both in the presence of O2 and in its absence.</p> Signup and view all the answers

How should specimens be collected to avoid contamination with normal flora?

<p>Specimens should be collected using sterile techniques and by needle aspiration to minimize contamination.</p> Signup and view all the answers

What are some characteristics of Robertson’s cooked meat media?

<p>It should be freshly prepared, used within 2 weeks, supplemented with special nutrients, and contain reducing agents.</p> Signup and view all the answers

List two examples of non-spore forming anaerobes.

<p>Bacteroides and Lactobacillus are examples of non-spore forming anaerobes.</p> Signup and view all the answers

What types of infections are commonly associated with anaerobic non-spore forming Gram negative bacilli?

<p>Common infections include intraabdominal infections, postoperative wound infections, and lung abscesses.</p> Signup and view all the answers

Identify a disease caused by anaerobic Gram positive bacilli.

<p>Dental caries is a disease caused by anaerobic Gram positive bacilli like Lactobacilli.</p> Signup and view all the answers

What is the typical arrangement of anaerobic non-spore forming Gram positive bacilli?

<p>They are typically arranged in chains.</p> Signup and view all the answers

Why is it important to heat or steam anaerobic media before use?

<p>Heating or steaming at 80°C for 2 minutes expels dissolved oxygen, creating an optimal anaerobic environment.</p> Signup and view all the answers

What type of hemolysis is produced by Clostridium perfringens on blood agar?

<p>Clostridium perfringens produces β-hemolysis on blood agar.</p> Signup and view all the answers

What are the morphology characteristics of Clostridium perfringens?

<p>Clostridium perfringens are Gram positive bacilli, non-motile, and have subterminal spores.</p> Signup and view all the answers

How is gas gangrene diagnosed in the laboratory?

<p>Gas gangrene is diagnosed by culturing a lacerated wound swab on Robertson cooked meat medium and then subculturing on Neomycin blood agar under anaerobic conditions.</p> Signup and view all the answers

What test indicates the lecithinase activity of Clostridium perfringens?

<p>The lecithinase activity of Clostridium perfringens is indicated by the formation of opaque zones on egg yolk medium.</p> Signup and view all the answers

What does a positive Nagler’s reaction indicate?

<p>A positive Nagler's reaction indicates the presence of α-toxin as the antibody inhibits activity around the streak.</p> Signup and view all the answers

Describe the gelatin liquefaction characteristics of Clostridium perfringens.

<p>Clostridium perfringens slowly liquefies gelatin.</p> Signup and view all the answers

What are the typical culture conditions for Clostridium perfringens?

<p>Clostridium perfringens is cultured anaerobically at 37 °C for 2-3 days on media like Neomycin blood agar.</p> Signup and view all the answers

What is the significance of stormy clot formation in litmus milk for Clostridium perfringens?

<p>Stormy clot formation in litmus milk suggests the presence of Clostridium perfringens due to its ability to coagulate milk proteins.</p> Signup and view all the answers

What is the Gram stain result and morphology of Listeria monocytogenes?

<p>Listeria monocytogenes is Gram positive bacilli arranged in short chains.</p> Signup and view all the answers

What type of growth pattern does Listeria monocytogenes exhibit on blood agar?

<p>It produces a narrow zone of beta hemolysis on blood agar.</p> Signup and view all the answers

What are the differentiating tests to identify Listeria monocytogenes from Diphtheroids?

<p>Listeria monocytogenes is catalase positive, beta hemolytic, and exhibits tumbling motility.</p> Signup and view all the answers

What is the primary pathogenic significance of Haemophilus influenzae?

<p>Haemophilus influenzae is the most common cause of acute bacterial meningitis in young children.</p> Signup and view all the answers

Describe the growth requirements for Haemophilus influenzae.

<p>Haemophilus influenzae requires factors X and V, growing best on chocolate agar at 35-37°C.</p> Signup and view all the answers

What is the morphological characteristic of Haemophilus influenzae observed under a microscope?

<p>Haemophilus influenzae appears as pleomorphic, gram-negative coccobacilli.</p> Signup and view all the answers

How is Listeria monocytogenes identified using a microscopy technique?

<p>It can be identified by a direct smear showing gram positive bacilli with tumbling motility.</p> Signup and view all the answers

What factors contribute to the pathogenicity of Haemophilus influenzae?

<p>The polysaccharide capsule of Haemophilus influenzae aids in its virulence.</p> Signup and view all the answers

Study Notes

Bacterial Culture Methods and Diagnoses

  • N. meningitidis carriers: Specimens are nasopharyngeal swabs (West swab). Culture specimens on Thayer martin medium for 24 hours at 37°C with 5-10% CO2.

Moraxella catarrhalis (commensal neisseria)

  • Growth: Grows at room temperature and on nutrient agar at 37°C. Growth occurs on chocolate agar in 24 hours.
  • Diseases: Causes otitis media, sinusitis, and pneumonia
  • Specimen: Ear discharge or sputum
  • Microscopy: Gram negative diplococci
  • Culture: On nutrient agar
  • Colony Characteristics: Dry, greyish-white, opaque colonies. Colonies "scoot" across the media when pushed with a loop.
  • Biochemical Reactions: Identified by biochemical reactions. No acid production from glucose, maltose, lactose, and sucrose (or variable).

Commensal Neisseria

  • Location: Present in throat, nasopharynx, nose, mouth, and less frequently external genitalia
  • Misidentification: Can be mistaken for Neisseria meningitidis

Corynebacterium diphtheriae

  • Morphology: Gram-positive bacilli, non-motile, non-capsulated, non-spore forming. Bacilli arranged in Chinese letter patterns. Beaded appearance due to metachromatic granules (phosphate granules). Methylene Blue stains the bacilli, but not the granules.
  • Growth Requirements: Aerobic bacteria that grow best on Loeffler's serum at 37°C. Will also grow on blood agar.
  • Virulence: Depends on the production of exotoxin.

Diphtheria

  • Transmission: Transmitted by oral droplets or physical contact. Also transmitted by saliva.
  • Source: Case or carrier
  • Causative Organism: Corynebacterium diphtheriae
  • Disease Pathogenesis: Released exotoxin disseminates through the blood, affecting the heart, muscle, peripheral nerves, adrenals, and kidneys.
  • Symptoms: Characterized by sore throat, low-grade fever, and pseudomembrane on tonsils. Removal of the membrane causes bleeding and toxin spread. Respiratory obstruction and suffocation may occur.
  • Clinical Signs: Low-grade fever, bull neck, sore throat, difficulty swallowing, severe myocarditis

Laboratory Diagnosis of Diphtheria

  • Specimen: Throat swabs/membrane exudates
  • Direct Smears: Gram stain reveals gram positive bacilli
  • Cultures: Incubated on Loeffler's serum
  • Colonial Morphology Identification: Beaded gram positive bacilli arranged in Chinese letters
  • Subsequent Cultures: Blood agar (excludes Str. Pyogenes)
  • Biotyping: Blood tellurite agar differentiate gravis, mitis, and intermedius
  • Toxigenicity Test: Elek's test
  • ELISA: Used to detect diphtheria toxin in the specimen
  • Carrier Diagnosis: Throat or nasal swaps are tested for toxigenicity to diagnose carriers.

Diphtheroids

  • Description: Normal flora of the mucous membrane in the throat, skin, respiratory tract
  • Morphology/characteristics: Gram-positive bacilli, non-spore-forming, non-motile, arranged in angles or palisades.
  • Disease associations: Urinary tract infection, Bacteraemia, Osteomyelitis

Tuberculosis

  • Causative Organism: M. tuberculosis
  • Morphology/Characteristics: Acid-fast bacilli (thin straight/curved rods)
  • Growth Characteristics: Grows slowly, typically 2-8 weeks on egg-selective medium (Lowenstein-Jensen medium). 6-8% CO2 enhances growth.
  • Virulence: Ability to survive intracellularly inside macrophages
  • Other characteristics: Rapid grow on liquid medium form serpentine form, Acid-fast bacilli in chains due to the cord factor
  • Learning Outcomes: The student will be able to know morphology and growth of M. tuberculosis. Recognize clinical forms of tuberculosis, understand cell-mediated immunity in tuberculosis; Analyze tuberculin test results; know and perform Quanti-Feron test; diagnose tuberculosis using laboratory techniques; understand prevention and treatment strategies
  • Typical vs. saprophytic M. avium, M. kansasii, M. intracellulare: Rapid grower or slow grower, Pigmented or non-pigmented colonies, acid-fast/alcohol-fast staining characteristics

Mycobacteria

  • Description: Acid-fast bacilli, Aerobic, non-spore forming organisms. Cell wall contains mycolic acids, making it impermeable to most stains.
  • Medically Important Species: M. tuberculosis, M. bovis, M. leprae, atypical mycobacteria (NTM: M. avium, M. kansasii, M. intracellulare), environmental saprophytic mycobacteria (like M. smegmatis)
  • Growth Requirements: Grows slowly (weeks) on egg-selective medium (Lowenstein-Jensen medium) 6-8% CO2 inhance growth.
  • Virulence Factors: The ability to survive inside macrophages

Bacillus Group (Bacillus anthracis)

  • Morphology: Large aerobic, Gram-positive, spore-forming bacilli
  • Pathogenic Species: Bacillus anthracis (anthrax)
  • Saprophytic Species: Anthracoids (often found in water, soil, and air). Some strains can be pathogenic (e.g., B. cereus, food poisoning agent)
  • Bacillus cereus: Gram-positive bacilli, non-capsulated, motile, spore-forming
  • Lab Identification: Gram-stained smears show Gram-positive bacilli with square/bamboo stick ends arranged in chains, and surrounded by an unstained halo capsule. Organisms appear blue while capsule is pink using polychrome methylene blue stain, non-hemolytic colonies. The specimen shows inverted fire-tree appearance on gelatin medium.

Anaerobic Non-Spore Forming Bacteria

  • Definition: Do not need oxygen.
  • Classification:
    • Gram negative bacilli (Bacteroides)
    • Gram positive bacilli (Lactobacillus, Actinomyces)
  • Important Characteristics: These organisms do not survive in the presence of oxygen
  • Key Organisms: Bacteroides, Lactobacillus, and Actinomyces
  • Gram Negative Bacilli: The specimens are normal inhabitants of bowel , vagina and oral cavities
    • Intraabdominal infection
    • Post operative wound infection following abdominal operation
    • Puerperal sepsis
    • Periodontitis
    • Lung abscess

Anaerobic Spore Forming Bacteria (Clostridium)

  • Characteristics: Gram-positive, spore forming, anaerobic bacteria.
  • Habitat: Intestinal tract, soil, water
  • Important Species: C. tetani, C. perfringens (gas gangrene), C. botulinum, and C. difficile
  • C. Perfringens: Gram-positive bacilli, non-motile, non-capsulated, subterminal spores. Grows beta-hemolytic colonies on blood agar, produce thin film and swarming. Can be diagnosed by motility, catalase and indole test. Gelatin liquefaction test, stormy clot formation with litmus milk, and opaque zones on egg yolk medium.
  • C. Tetani: Gram-positive bacilli, anaerobic, non-capsulated, drumstick shape, terminal spores in culture
  • Lab Identification: Gram stain shows gram positive bacilli with drumstick appearance. Colonies on Robertson cooked meat medium grow. subculture on blood agar and incubated anaerobically. Produce a thin-film or swarming appearance. Are a-hemolytic followed by β-hemolysis , motility. Gelatin liquefaction
  • C. difficile: Antibiotic-associated diarrhea

Listeria monocytogenes

  • Characteristics: Gram-positive bacilli, arranged in short chains, non-capsulated, motile, non-spore forming, cold tolerant.
  • Disease Association: Neonatal listeriosis and adult listeriosis.
  • Culture: Grows well on blood agar. Colonies produce a narrow zone of hemolysis similar to Group B Streptococcus.
  • Identification: Direct smear of specimen shows gram-positive bacilli, catalase-positive, tumbling motility at 25°C (umbrella type).
  • Differentiation: Differentiated from other bacteria based on various tests (e.g., catalase+, motility, esculin hydrolysis).

Haemophilus influenzae

  • Description/Characteristics: Gram-negative pleomorphic coccobacilli. Fastidious, requiring specific growth factors (X and V factors) like hemin and NAD.
  • Disease Association: Acute bacterial meningitis in young children. Other potential diseases: meningitis, urethritis, associated with eye infections, chancroid.
  • Culture: Grows on chocolate agar (both X and V factors are present). Requires 35-37°C, pH 7.6, and 5% CO2. Will not grow on normal blood agar.
  • Identification/Diagnosis:
    • Direct stain of CSF/pus/specimen/body fluid will reveal pleomorphic gram-negative coccobacilli
    • Capsular polysaccharide detection by quellung or other methods
    • Culture: Use chocolate or other enriched media, colonies are identified by their morphology

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This quiz covers various aspects of pathogenic bacteria including Neisseria meningitidis, Corynebacterium diphtheriae, and Moraxella catarrhalis. It tests knowledge on their diagnostic methods, culture conditions, morphological characteristics, and virulence factors. Perfect for students in microbiology or medical programs.

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