Microbiology Quiz on Mollicutes and Actinomycetes
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Questions and Answers

What is a distinguishing feature of the mollicutes group?

  • Lack of a definite cell wall (correct)
  • Presence of a rigid cell wall
  • Resistance to osmotic lysis
  • Sensitivity to penicillin
  • Which mycoplasma is most commonly associated with atypical pneumonia in humans?

  • Mycoplasma hominis
  • Mycoplasma genitalium
  • Mycoplasma pneumoniae (correct)
  • Mycoplasma mycoides
  • What potential complication is associated with the use of an IUCD for more than one year?

  • Chronic pelvic pain
  • Endometritis (correct)
  • Uterine cancer
  • Ectopic pregnancy
  • What is the appearance of colonies developed by mollicutes on solid culture media?

    <p>Nipped or 'fried-egg' shape (A)</p> Signup and view all the answers

    What is the recommended initial treatment approach for infections caused by mollicutes, given their resistance profile?

    <p>Empirical penicillin therapy (C)</p> Signup and view all the answers

    What is a characteristic feature of Actinomycetes?

    <p>Branching, elongated G+ rods (B)</p> Signup and view all the answers

    Which disease is primarily associated with Actinomyces israelii?

    <p>Chronic cellulitis (A)</p> Signup and view all the answers

    What is the standard treatment for Actinomycosis?

    <p>Penicillin G (A)</p> Signup and view all the answers

    Which of the following is an incorrect statement about Nocardia species?

    <p>Most cases of Nocardia infection are documented as outbreaks. (A)</p> Signup and view all the answers

    How does Nocardia typically infect humans?

    <p>Through skin breaches due to environmental exposure (D)</p> Signup and view all the answers

    What type of bacteria is Mycoplasma pneumoniae classified as?

    <p>Gram-positive, wall-deficient bacterium (B)</p> Signup and view all the answers

    What is the primary ecological niche for Nocardia species?

    <p>Soil and organic matter (D)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of aerobic actinomycetes?

    <p>They are G- cocci. (A)</p> Signup and view all the answers

    What is the primary mechanism by which Mycoplasma pneumoniae attaches to the respiratory epithelium?

    <p>Through an attachment factor called P1 (C)</p> Signup and view all the answers

    Which of the following clinical manifestations is least associated with Mycoplasma infections?

    <p>Rigors and severe sweating (C)</p> Signup and view all the answers

    What best describes the growth characteristics of Mycoplasma pneumoniae?

    <p>Aerobic and very slow-growing (D)</p> Signup and view all the answers

    During Mycoplasma pneumoniae infection, what is the role of CARDS toxin?

    <p>To interfere with ciliary action and induce inflammation (C)</p> Signup and view all the answers

    Which age group is most commonly affected by Mycoplasma pneumoniae infections?

    <p>Children aged 5 to 15 years (A)</p> Signup and view all the answers

    What type of pneumonia is primarily caused by Mycoplasma pneumoniae?

    <p>Atypical pneumonia (B)</p> Signup and view all the answers

    How is Mycoplasma pneumoniae commonly transmitted between individuals?

    <p>By droplet infection of nasopharyngeal secretions (C)</p> Signup and view all the answers

    In what condition is the Mycoplasma pneumoniae infection underestimated due to its similarity to other illnesses?

    <p>Viral respiratory infections (A)</p> Signup and view all the answers

    Which of the following is a consequence of M.pneumonia infection affecting epithelial cells?

    <p>Desquamation of the mucosal surface (C)</p> Signup and view all the answers

    What is the duration of pathogen harboring in individuals recovered from Mycoplasma infection?

    <p>2 months or more (B)</p> Signup and view all the answers

    What is the primary method used to conclusively identify Mycoplasma pneumoniae?

    <p>Staining colonies with a fluorescent probe (A)</p> Signup and view all the answers

    Which statement accurately describes L-forms of bacteria?

    <p>L-forms result from impaired cell wall synthesis due to antibiotics. (B)</p> Signup and view all the answers

    What distinguishes pleuropneumonia-like organisms (PPLO) from other bacteria?

    <p>Production of extremely small colonies on agar (C)</p> Signup and view all the answers

    What is a significant characteristic of Mycoplasma and L-forms regarding their cellular structure?

    <p>They lack traditional cell wall components. (C)</p> Signup and view all the answers

    In what way does multiplication of bacteria without a rigid cell wall differ from typical bacteria?

    <p>It does not involve the formation of a septum. (C)</p> Signup and view all the answers

    What is the key feature of T-strains in mycoplasmas?

    <p>They form very tiny colonies on agar plates. (A)</p> Signup and view all the answers

    What is a common environmental condition that can lead to the formation of L-forms?

    <p>Presence of high salt concentrations (A)</p> Signup and view all the answers

    Which statement is FALSE regarding Mycoplasma pneumoniae compared to L-forms?

    <p>L-forms are restricted to specific bacterial species. (B)</p> Signup and view all the answers

    What are the conditions that can lead to bacteria becoming L-forms?

    <p>Antibiotic treatment and high salt concentration (D)</p> Signup and view all the answers

    What is a distinguishing factor of the cell membranes in mycoplasmas?

    <p>They have high concentrations of sterols. (B)</p> Signup and view all the answers

    What structural feature distinguishes mycoplasmas from typical bacteria?

    <p>Absence of a cell wall (A)</p> Signup and view all the answers

    Which staining method would be ineffective in identifying mycoplasmas due to their cell wall structure?

    <p>Gram stain (D)</p> Signup and view all the answers

    What is the typical diameter range of mycoplasmas?

    <p>0.15 u to 0.30 u (B)</p> Signup and view all the answers

    Which antibiotic is ineffective against mycoplasmas due to their lack of a cell wall?

    <p>Vancomycin (A)</p> Signup and view all the answers

    What type of environment do most mycoplasmas require for growth?

    <p>Facultatively anaerobic (B)</p> Signup and view all the answers

    Which unique characteristic is associated with Mycoplasma pneumoniae's mode of movement?

    <p>Gliding motility (D)</p> Signup and view all the answers

    What component is crucial for the growth and survival of mycoplasmas?

    <p>Sterols (D)</p> Signup and view all the answers

    Which toxin is produced by Mycoplasma pneumoniae that is associated with respiratory diseases?

    <p>CARDS toxin (C)</p> Signup and view all the answers

    How does the plasma membrane of mycoplasmas compare to that of animal cells?

    <p>It contains cholesterol (C)</p> Signup and view all the answers

    Which of the following statements is true regarding the genetic material of mycoplasmas?

    <p>They have unique DNA sequences distinct from known prokaryotes (A)</p> Signup and view all the answers

    Flashcards

    Actinomycetes

    Elongated, branching, slow-growing gram-positive rods that are often found in the oral and intestinal flora. They can cause chronic cellulitis and draining sinuses.

    Actinomycosis

    A disease caused by Actinomycetes characterized by chronic cellulitis and the formation of draining sinuses. Most infections are caused by A. israelii.

    Nocardia

    A genus of aerobic actinomycetes that are gram-positive, branching, filamentous bacteria. They are slow-growing, poorly stained, and can cause a variety of infections.

    Nocardia asteroides complex

    A type of Nocardia that is responsible for about 80% of noncutaneous (not skin-related) Nocardia infections, including systemic and CNS nocardiosis.

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    Nocardia farcinica

    A type of Nocardia that is less common but more virulent than Nocardia asteroides complex. This makes it more difficult to treat with antibiotics.

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    Nocardia: Ecology & Epidemiology

    Nocardia can be found in a wide range of environments, including soil, water, and organic matter. It is particularly prevalent in tropical and subtropical regions.

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    Nocardia: Transmission

    Most cases of Nocardia infections are sporadic, meaning they don't spread easily from person to person. However, outbreaks can occur due to contamination of the hospital environment or medical equipment.

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    Nocardia: Treatment & Prevention

    Nocardia infections can be difficult to treat because some strains are resistant to antibiotics. Penicillin G is a common treatment option, but it is important to consult a healthcare professional for proper diagnosis and treatment.

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    What are Mycoplasmas?

    Mycoplasmas are unique bacteria lacking a rigid cell wall throughout their life cycle, making them smaller and adaptable than typical bacteria.

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    Why are Mycoplasmas resistant to some antibiotics?

    The absence of a cell wall in mycoplasmas makes them resistant to antibiotics that target cell wall synthesis, like penicillin and vancomycin.

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    What is the unique structure of Mycoplasmas?

    Mycoplasmas are characterized by a tri-layered plasma membrane containing cholesterol, providing structural support and flexibility.

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    How did Mycoplasmas evolve?

    Mycoplasmas are believed to have evolved from Gram-positive bacteria through a process called reductive evolution, where they lost their cell wall.

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    What is the PPO?

    The pleuropneumonia organism (PPO) was the first identified Mycoplasma species, linked to a respiratory disease in cattle.

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    How large are Mycoplasmas?

    Mycoplasmas are known for their small size, typically ranging from 0.15 to 0.30 micrometers in diameter.

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    Describe the shape of Mycoplasmas.

    Mycoplasmas are incredibly flexible in shape, appearing as spheres, rods, spirals, or even ring forms. This is due to their lack of rigid cell wall.

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    How does Gram staining affect Mycoplasmas?

    Mycoplasmas cannot be stained by Gram staining techniques because they lack a cell wall.

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    What is the terminal organelle?

    Mycoplasma pneumoniae, a common cause of respiratory infections, has a specialized attachment organelle called a terminal organelle.

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    What toxin does Mycoplasma pneumoniae produce?

    Mycoplasma pneumoniae produces a toxin called CARDS toxin, which can cause respiratory problems.

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    What is Mycoplasma pneumoniae?

    A type of bacteria that causes pneumonia, tracheobronchitis, and pharyngitis. It is known for its slow growth and ability to attach to respiratory epithelium.

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    How does Mycoplasma pneumonia infect the respiratory system?

    Mycoplasma pneumoniae infects the respiratory system by attaching to the cilia and microvilli of epithelial cells using a protein called P1. It causes inflammation and destruction of epithelial cells, affecting the respiratory lining.

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    What is 'walking pneumonia'?

    A characteristic of Mycoplasma pneumoniae is that a low dose of the bacteria can cause a mild form of pneumonia, often mistaken for a viral infection.

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    How does Mycoplasma pneumoniae infection spread?

    Mycoplasma pneumoniae is usually spread through respiratory droplets released when an infected person coughs or sneezes. Close contact with infected individuals increases the risk of infection. It is common in crowded settings like schools and military groups.

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    What are the symptoms of Mycoplasma pneumonia?

    Mycoplasma pneumoniae can cause a range of symptoms, including fever, cough, sore throat, headache, and chills. The cough is usually non-productive (without phlegm).

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    How does Mycoplasma pneumonia cause damage to the respiratory system?

    Mycoplasma pneumoniae can cause damage to the respiratory lining by producing a toxin called CARDS toxin. This toxin interferes with ciliary action and causes cells to weaken and detach.

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    What is unusual about the duration of Mycoplasma pneumoniae infection?

    A significant characteristic of Mycoplasma pneumoniae is its ability to persist in the body for extended periods, even after recovery. Individuals can remain carriers for weeks or months.

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    Who is most susceptible to Mycoplasma pneumonia?

    Although Mycoplasma pneumoniae is a major cause of pneumonia in teenagers, it is uncommon in infants under 6 months of age. This suggests a window of vulnerability.

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    Is there a specific season for Mycoplasma pneumonia?

    Mycoplasma pneumoniae infections do not show a distinct seasonality. People can get infected throughout the year, unlike some respiratory infections that peak during specific seasons.

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    How is Mycoplasma pneumonia diagnosed and treated?

    Mycoplasma pneumoniae infections can be diagnosed by laboratory tests, such as culture and PCR. Treatment typically involves antibiotics, but some strains can develop resistance.

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    Perianal Actinomycosis

    A type of bacterial infection that resembles inflammatory bowel disease, characterized by recurrent wounds or fistulas that fail to heal. It often involves the perianal region, the area surrounding the anus. This can be a tricky diagnosis and requires special imaging and treatment with antibiotics.

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    Kidney, Ureter, and Bladder (KUB) Infections

    An infection of the kidneys, ureters, and bladder. These infections can occur anywhere along the urinary tract and can lead to abscess formation. Bladder involvement is often a complication of pelvic infections. The disease can be diagnosed with urine cultures and imaging studies.

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    Pelvic Actinomycosis

    A pelvic infection that can occur in women, often associated with the presence of an intrauterine contraceptive device (IUCD). Classic symptoms are usually subtle and include fever, weight loss, abdominal pain, and abnormal vaginal bleeding.

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    Diagnosis of Actinomycosis

    A technique used to diagnose actinomycosis, involving the aspiration of infected material and subsequent microscopic examination. This helps to identify the characteristic sulfur granules, which are small, yellowish clumps of bacteria. A biopsy can also show characteristic club-shaped lesions. Penicillin is often the antibiotic of choice.

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    Mollicutes (Mycoplasmas)

    A group of bacteria characterized by the absence of a rigid cell wall, making them resistant to certain antibiotics like penicillin. This unique feature makes them difficult to fight and can cause various infections in humans and animals.

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    Mycoplasma pneumoniae infection

    A type of bacterial infection, characterized by a lack of a rigid cell wall, that can be identified using fluorescently labelled antibodies.

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    Nucleic Acid Probes for M. pneumoniae

    A technique used to identify Mycoplasma pneumoniae by using probes that bind only to specific DNA sequences found in this bacterium.

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    L-forms of bacteria

    Bacteria with altered, defective, or absent cell walls, often appearing as small, filterable, protoplasmic elements.

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    Difference between L-forms and Mycoplasma

    Bacterial forms that lack a rigid cell wall during at least a part of their life cycle, setting them apart from true Mycoplasma.

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    Pleuropneumonia-like organisms (PPLO)

    Diverse group of bacteria with similar morphology and growth characteristics, often referred to as PPLO (pleuropneumonia-like organisms).

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    T-strains of Mycoplasma

    A specific group of Mycoplasma that form very small colonies on agar plates, distinguishable by their tiny size.

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    Multiplication of Mycoplasma

    The process of replication in these bacteria differs from typical bacteria, as they lack a rigid cell wall and use a different method of division.

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    Antibiotic Resistance in Mycoplasma

    Their lack of a rigid cell wall makes them resistant to certain antibiotics that target cell wall synthesis.

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    Morphological diversity of Mycoplasma

    These bacteria can exhibit various shapes, depending on the environment and growth conditions. They can be spherical, filamentous, or even have a pleomorphic appearance.

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    Lack of sterols in Mycoplasma

    Mycoplasma lack sterols, a key component found in the cytoplasmic membrane of other bacteria, further defining their unique characteristics.

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    Study Notes

    Microbiology Lecture 13: Actinomycetes, Mycoplasma, and Cell Wall-Defective Bacteria

    • Lecture covers Actinomycetes, norcardiosis, actinomycetoma, Mycoplasma, and cell wall-defective bacteria.

    Actinomycetes

    • Elongated Gram-positive rods
    • Branching morphology
    • Slow growth (4-10 days)
    • Microaerophilic or strictly anaerobic
    • Source: oral/intestinal endogenous flora
    • Disease: chronic cellulitis, draining sinuses
    • Lesion: composed of inflammatory sinuses that discharge to the surface
    • Major cause: Actinomyces israelii

    Actinomycosis

    • Part of normal intestinal microflora
    • Endogenous infection; growth requires displacement into tissue (surgery, trauma, etc.)
    • Sinus tracts contain pus and sulfur granules
    • Little evidence of immunity
    • Treatment: Penicillin G

    Aerobic Actinomycetes (Genus)

    • Aerobic Gram-positive branching filamentous bacteria
    • Slow growth (2-3 days)
    • Poorly stained
    • Subgroups:
      • Nocardia
      • Actinomadura
      • Streptomyces
      • Rhodococcus
      • Gordonia
      • Tsukamurella
      • Tropheryma whipplei

    Nocardia: History

    • Edmond Nocard, 1888
    • Aerobic actinomycetes isolated from cattle with bovine farcy

    Nocardia: Growth on Blood Agar

    • (Image of bacterial growth on blood agar)

    Nocardia: Species and Human Infections

    • At least 13 species cause human infections
    • 7 most important species:
      • Nocardia asteroides complex: 80% of noncutaneous; most systemic & CNS nocardiosis
      • Nocardia farcinica: Less common, more virulent, more antibiotic-resistant
      • Nocardia nova
      • Nocardia brasiliensis: Skin, cutaneous, lymphocutaneous
      • Nocardia pseudobrasiliensis: Systemic infections, CNS
      • Nocardia otitidiscaviarum
      • Nocardia transvalensis

    Nocardia: Ecology & Epidemiology

    • Ubiquitous environmental saprophyte
    • Soil, organic matter, water
    • Tropical and subtropical regions (Mexico, Central and South America, Africa, India)
    • Nearly all cases are sporadic
    • Human-to-human transmission not documented
    • Animal-to-human transmission not documented
    • Outbreaks: Contamination of the hospital environment, solutions, or drug injection equipment
    • Occurrence in immunocompromised patients is increased
    • Transmission by: Inhalation and Skin
    • Risk of pulmonary or disseminated disease is associated with deficient cell-mediated immunity, such as in the following conditions: Alcoholism, Diabetes, Lymphoma, Transplantation, Glucocorticoid therapy, AIDS

    Nocardia: Pathogenesis

    • Neutralization of oxidants
    • Prevention of phagosome-lysosome fusion
    • Prevention of phagosome acidification
    • Mycolic acid polymers associated with virulence

    Nocardia: Clinical Manifestations

    • 4 main forms:
      • Lymphocutaneous syndrome
      • Pulmonary nocardiosis (pneumonia)
      • CNS nocardiosis (brain abscess)
      • Disseminated disease (Eyes (retina), Skin, Subcutaneous tissue, Kidneys, Joints, Bone, Heart)

    Lymphocutaneous Syndrome

    • Ubiquitous in soil; inoculation injuries or contaminated abrasions from insect and animal bites
    • N. brasiliensis is most common
    • N. asteroides is typically self-limited
    • Disease duration: Days to months
    • Common site: Distal limb

    Pulmonary Disease

    • Subacute (more acute in immunocompromised); cough with small amounts of thick, purulent sputum; fever, anorexia, weight loss, malaise
    • Endobronchial inflammatory mass
    • Lung abscess
    • Cavitary disease
    • Inadequate therapy may lead to progressive fibrotic diseases
    • Cerebral imaging is recommended in all cases of pulmonary and disseminated nocardiosis.

    CNS Nocardiosis (Brain Abscess)

    • Common presentations: insidious presentations mistaken for neoplasia; granulomas & abscesses, often affecting the cerebral cortex, basal ganglia, or midbrain.
    • Less commonly, spinal cord or meninges may be affected
    • Brain tissue diagnosis in pulmonary nocardiosis may NOT be needed
    • Cerebral biopsy: common in immunocompromised patients

    Laboratory Diagnosis (Nocardia)

    • Gram-positive, beaded, and branching filaments
    • Standard blood culture may take 48-hours to several weeks
    • Colonies can be grown from sputum from patients with underlying lung disease
    • Deep-seated or disseminated disease that fails initial therapy and relapses after therapy warrants an alternative treatment based on susceptibility testing
    • Susceptibility to sulfonamide may be crucial in deciding on a treatment or treatment modification

    Management (Nocardia): Medication

    • Sulfonamides: Mainstay of therapy (N. brasiliensis, N. asteroides complex)
    • Severely ill patients with CNS or disseminated infections/immunocompromised patients require additional drugs such as Amikacin and Carbapenem OR 3rd generation cephalosporin.

    Mycoplasma (Mollicutes)

    • Smallest known free-living organisms
    • Lack a cell wall
    • Not stained by Gram stain; more pleomorphic/plastic than eubacteria
    • Staining: Giemsa stain (pleomorphic cocci, rods, spirals, and sometimes as hollow ring forms) ; diameter: 0.15 μ to 0.30 μ
    • Genera: Mycoplasma, Ureaplasma, Acholeplasma, Anaeroplasma, Spiroplasma

    Mycoplasma: Structure

    • Enclosed by a limiting membrane similar to animal cells
    • Contain sterols; cytoplasm with ribosomes, but lack mesosomes
    • No nuclear membrane
    • Some strains have amorphous material on outer membrane, suggesting a capsule

    Mycoplasma: Structure (Terminal Organelle)

    • Terminal organelle; mediates attachments and gliding motility
    • Proteins (P1, P30) responsible for attachment

    Mycoplasma: Growth and Replication

    • Requires sterols for growth
    • Cultivation: Can be grown on laboratory media
    • Most are facultatively anaerobic; M. pneumoniae is an exception (aerobic)
    • Replication is controversial; replication time: 1-6 hours

    Mycoplasma: Toxin Production

    • M. pneumoniae produces an ADP-ribosylating toxin: Commonly Associated Respiratory Disease Syndrome (CARDS) toxin
    • Lab: Colonies bind red blood cells to agar plate cultures (hemadsorption)

    Mycoplasma: Clinical Features

    • Also known as Eaton's agent
    • Extracellular pathogen
    • Attaches to respiratory epithelium by an attachment factor: P1.
    • Interacts with glycoprotein receptors on epithelial cell surface
    • Ciliostasis is followed by epithelial cell destruction.

    Mycoplasma Pneumonia: Clinical Features

    • Low infectious dose
    • Walking pneumonia
    • Frequently confused with virus infection (primary atypical clinical manifestations)
    • Tracheobronchitis
    • Pharyngitis (use differential diagnosis from strep throat)

    Mycoplasma Infections: Spread

    • Worldwide; all ages
    • Transmission by droplets of nasopharyngeal secretions
    • Important in military personnel
    • Epidemics: Intervals of 4-6 years
    • Retained by pathogens in recovered patients; common in 5-15-year-old adolescents/teenagers more than in older groups of patients
    • Infections in children under 6 months are uncommon
    • No seasonal incidence

    Mycoplasma: Clinical Manifestations

    • Generalized aches and pains
    • Fever (usually 102°F)
    • Cough (frequently, non-productive)
    • Sore throat (nonexudative pharyngitis)
    • Headache/myalgias
    • Chills, but not rigors
    • Nasal congestion with coryza
    • Earache
    • General malaise

    Mycoplasma: Pathogenesis

    • Infection involves the trachea, bronchi, and bronchioles (peribronchiolar tissues)
    • M. pneumoniae initially attaches to cilia and microvilli of cells lining bronchial epithelium
    • Attachment mediated by protrusion associated proteins (e.g., P1, P30); other proteins bind to extracellular matrix (e.g., fibronectin)
    • CARDS toxin interferes with ciliary action, leading to nuclear vacuolization and fragmentation of tracheal epithelial cells
    • Inflammation and desquamation of affected mucosa

    Radiological Presentation

    • Variable presentation on CXR; commonly presents as bilateral lower lobe consolidation with small pleural effusions
    • Initially, as partly mottled, partly node-like peribronchial opacities with gradual development to involve whole segments or lobes

    Mycoplasma: Immunity

    • Incomplete immunity
    • Re-infection may occur
    • Clinical presentation appears more severe in older children
    • Many clinical manifestations likely caused by immune response; not due to organism invasion

    Urethritis

    • Half of urethral infections are not caused by Chlamydia or N. gonorrhoeae
    • Causes: Mycoplasma hominis and Ureaplasma
    • Diagnosis through culturing (fried egg colonies on medium containing sterols), serology (complement fixation tests, hemagglutination)

    Laboratory Diagnosis (Mycoplasma)

    • Culture techniques: Mycoplasma from specimen (sputum, mucous membrane) inoculated in liquid/solid media with serum, yeast extract, and penicillin to inhibit contaminating bacteria
    • Cultural Characteristics: Lack a cell wall, but grow on solid media as small, transparent colonies (fried-egg appearance). Growth period; 2 days-several weeks

    Serology Diagnosis (Mycoplasma)

    • Complement fixation
    • Hemagglutination (cold agglutinin test, positive for M. pneumoniae (Primary Atypical) Pneumonia; agglutination of human O group erythrocytes at 4°C, reversible at 37°C.
    • Hemabsorption & B-hemolysis of guinea pig red blood cells

    Identification (Mycoplasma)

    • Conclusive identification through staining colonies with fluorescein-labeled antibody

    Mycoplasma Nucleic Acid Probes

    • Specific recombinants to oligonucleotide sequences found exclusively in M. pneumoniae

    L-forms

    • Some bacteria readily form variants that can replicate as filterable protoplasmic elements lacking a cell wall– called L-forms
    • L-forms formation may occur when cell wall synthesis is impaired by antibiotic treatment or high salt concentration.

    L-Forms vs Mycoplasma

    • L-Forms: Contain a rigid cell wall at least at one stage in life cycle; lack sterols in cytoplasmic membrane
    • Mycoplasma: No cell wall; contain sterols in cytoplasm membrane

    Pleuropneumonia-like organisms (PPLO)

    • Many organisms with similar morphological characteristics and cultural properties
    • Common reference: As PPLO.
    • A group of Mycoplasmas create extremely small colonies (T-strains) on agar plates

    Multiplication (Mycoplasma)

    • Replication pattern differs due to the absence of a rigid cell wall; replication of typical bacteria starts with formation of a well-defined septum

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    Test your knowledge on the unique features of mollicutes and actinomycetes in this microbiology quiz. Explore characteristics, infections, and treatment approaches associated with these bacterial groups. Perfect for students and professionals interested in microbial pathogens.

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