Microbiology Quiz: Basics and Applications
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Questions and Answers

Which type of organism is Penicillium roqueforti?

  • Eukaryote (correct)
  • Akaryote
  • Virus
  • Prokaryote
  • What is a key characteristic of a eukaryotic cell?

  • The absence of a nucleus.
  • The presence of multiple chromosomes within a membrane-enclosed nucleus. (correct)
  • The existence of a single circular chromosome
  • The lack of internal membrane-bound structures.
  • Which of these scientists is credited with the first observation of bacteria?

  • Edward Jenner
  • Louis Pasteur
  • Alexander Fleming
  • Antonie Van Leeuwenhoek (correct)
  • What is one of the key roles of Penicillium roqueforti in cheese making?

    <p>Breakdown of casein and triglycerides (D)</p> Signup and view all the answers

    Which of the following is NOT considered a domain of life studied within microbiology?

    <p>Viruses (A)</p> Signup and view all the answers

    What process did Louis Pasteur promote?

    <p>The importance of germ theory (A)</p> Signup and view all the answers

    What are some potential applications of microbes within society?

    <p>All of the above (D)</p> Signup and view all the answers

    Which scientist discovered the phenomenon of transformation in bacteria?

    <p>Frederick Griffith (B)</p> Signup and view all the answers

    Which of these describes a mutualistic relationship?

    <p>Both organisms in the association benefit. (C)</p> Signup and view all the answers

    Which of the following is NOT typically associated with human infectious disease?

    <p>Archaea (A)</p> Signup and view all the answers

    Which of the following is a common method of microbial movement?

    <p>Water flow (C)</p> Signup and view all the answers

    What is a primary way Vibrio cholerae is transmitted to humans?

    <p>Consumption of contaminated water (A)</p> Signup and view all the answers

    How can the spread of Vibrio cholerae be prevented in communities?

    <p>Boiling water used for consumption, and improved sanitation. (B)</p> Signup and view all the answers

    Which of the following is typically spread through inhalation of aerosols?

    <p>Influenza (D)</p> Signup and view all the answers

    Where is Staphylococcus aureus commonly found in humans?

    <p>On skin and mucous membranes (A)</p> Signup and view all the answers

    Which of the following microorganisms is spread primarily through direct contact?

    <p>Staphylococcus aureus (C)</p> Signup and view all the answers

    Which characteristic of Staphylococcus aureus contributes to its survival on surfaces?

    <p>Its structure of the cell wall providing resistance to desiccation (C)</p> Signup and view all the answers

    Which of the following best describes the transmission of the Ebola virus?

    <p>Zoonotic or direct person-to-person contact (D)</p> Signup and view all the answers

    According to the content, animal contact can occur in several ways. Which of these does NOT describe a route of animal contact?

    <p>Through contaminated medical equipment (A)</p> Signup and view all the answers

    Which environmental factor is a major contributor to the spread of E. coli?

    <p>Faecal contamination of soil and water (C)</p> Signup and view all the answers

    Which of these is NOT a typical use of microbes?

    <p>Development of medical devices (C)</p> Signup and view all the answers

    Which vaccine is administered by subcutaneous injection?

    <p>MMR (B)</p> Signup and view all the answers

    Which of the following bacterial species is primarily responsible for the fermentation process in soy sauce production?

    <p><em>Pediococcus species</em> (B)</p> Signup and view all the answers

    What can be understood about disease transmission by studying the cell biology and biochemistry of microbes?

    <p>The way in which the disease can spread (D)</p> Signup and view all the answers

    Which of the following correctly describes the arrangement of Staphylococcus aureus bacteria?

    <p>Clusters of cocci (B)</p> Signup and view all the answers

    What is the typical size range for bacteria in diameter?

    <p>0.2 - 2.0 μm (D)</p> Signup and view all the answers

    Which of the following bacterial shapes is described as a curved bacillus?

    <p>Vibrio (B)</p> Signup and view all the answers

    Which of these bacteria is known to cause dental decay?

    <p><em>Streptococcus pyogenes</em> (B)</p> Signup and view all the answers

    What is the genus of the bacteria that exhibits a corkscrew shape?

    <p><em>Treponema</em> (B)</p> Signup and view all the answers

    Which of the following is an example of a beneficial use of bacteria?

    <p>Recycling of elements (C)</p> Signup and view all the answers

    Which of the following bacteria is described as being arranged in pairs?

    <p><em>Neisseria gonorrhoeae</em> (A)</p> Signup and view all the answers

    Which of the following is an example of a bacteria with a bacilli shape, arranged in chains?

    <p><em>Lactobacillus sake</em> (C)</p> Signup and view all the answers

    Which of the following best describes the function of fimbriae in bacterial cells?

    <p>Adhesion to surfaces and other cells. (D)</p> Signup and view all the answers

    What is the primary function of the cell membrane in bacteria?

    <p>Enclosing the cytoplasm and regulating passage of substances. (C)</p> Signup and view all the answers

    Which type of transport allows molecules to move across the cell membrane against their concentration gradient, using energy?

    <p>Active transport (D)</p> Signup and view all the answers

    What is the main component of the bacterial cell wall?

    <p>Peptidoglycan (A)</p> Signup and view all the answers

    In the peptidoglycan structure, what bonds link N-acetylglucosamine and N-acetylmuramic acid?

    <p>β(1-4) glycosidic bonds (B)</p> Signup and view all the answers

    What is the function of integral proteins in the cell membrane?

    <p>Forming pores for transport of molecules. (C)</p> Signup and view all the answers

    Which of the following is a characteristic feature of a Gram-positive cell wall?

    <p>A thick peptidoglycan layer containing teichoic acids (B)</p> Signup and view all the answers

    Which of the following is NOT a function of the bacterial cell membrane?

    <p>Storage of genetic materials. (D)</p> Signup and view all the answers

    Which of the following is a common component of the peptidoglycan in Staphylococcus aureus?

    <p>L-lysine (B)</p> Signup and view all the answers

    What is the primary role of the O-specific polysaccharide in lipopolysaccharide (LPS)?

    <p>To provide antigenic variation (C)</p> Signup and view all the answers

    Which of the following best describes the linkage between peptide chains in the peptidoglycan of E. coli?

    <p>Direct peptide link (B)</p> Signup and view all the answers

    Which of the following is characteristic of the outer membrane of Gram-negative bacteria?

    <p>It contains lipopolysaccharide (LPS) (C)</p> Signup and view all the answers

    What is the role of teichoic acid in the cell wall structure?

    <p>It is found in the peptidoglycan layer of gram-positive bacteria (A)</p> Signup and view all the answers

    What is the specific role of Lipid A in lipopolysaccharide (LPS)?

    <p>It is responsible for the toxic effects of Gram-negative bacteria (A)</p> Signup and view all the answers

    Which of the following is a key difference in the cell wall structure between Gram-positive and Gram-negative bacteria?

    <p>Gram-negative bacteria possess lipopolysaccharide (LPS), while Gram-positive bacteria do not (A)</p> Signup and view all the answers

    Which of the following human pathogens is considered a Gram-positive organism?

    <p><em>Staphylococcus aureus</em> (A)</p> Signup and view all the answers

    Flashcards

    What is microbiology?

    Microbiology is the scientific study of microscopic organisms, including bacteria, archaea, viruses, protists and akaryotes. These organisms play crucial roles in diverse environments like soil, water, and within living organisms.

    What is a eukaryote?

    Eukaryotes are organisms whose cells possess a true nucleus containing multiple chromosomes, a nuclear envelope, and membrane-bound organelles. They encompass animals, plants and fungi.

    What is a prokaryote?

    Prokaryotes are single-celled organisms lacking a true nucleus and other membrane-bound organelles. They have a simpler cellular structure compared to eukaryotes.

    What is an akaryote?

    Akaryotes are organisms lacking a nucleus altogether. They are often considered simpler than prokaryotes and include viruses and prions.

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    What is mutualism?

    Mutualism is a symbiotic relationship where both organisms involved benefit from the interaction. It's a win-win situation for both partners.

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    What is predation?

    Predation is an interaction where one organism (the predator) consumes another organism (the prey) for food.

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    What is nutrient cycling?

    Nutrient cycling is the continuous movement and transformation of essential nutrients within an ecosystem. Microbes play vital roles in this process, breaking down organic matter and releasing nutrients back into the environment.

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    What are microbial transfer/transmission routes?

    Microbial transfer/transmission routes refer to the pathways through which microorganisms can spread from one organism or location to another. These routes can include direct contact, air, water, food, or vectors like insects.

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    Nosocomial infection

    A bacterial infection spread through contact with contaminated surfaces, fluids, or animals.

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    Staphylococcus aureus

    A type of bacterium with a thick cell wall that makes it resistant to drying out.

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    Ebola virus

    A highly contagious viral disease that spreads through direct contact with infected bodily fluids.

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    Filoviridae

    A group of viruses that are known to cause severe diseases in humans.

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    Microbial utilization

    The process of microbes being used to create products for human benefit.

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    Microbially derived foods

    The use of microbial processes to produce foods like yogurt, cheese, and sauerkraut.

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    Intramuscular vaccination

    A type of vaccine administered into the muscle.

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    Vaccination

    The process of preventing disease by introducing a weakened or inactivated version of a microbe into the body.

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    Mutualism

    A close relationship between two organisms where both partners benefit. For example, nitrogen-fixing bacteria provide nitrogen to plants, while plants provide shelter and nutrients to bacteria.

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    Microbial Movement

    The movement of microorganisms between different locations or habitats. It can occur through various means like air, water, direct contact, or human-associated processes.

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    Fecal Contamination

    The contamination of water sources with fecal matter, leading to the spread of infectious diseases like cholera.

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    Vibrio cholerae

    A bacterium responsible for causing gastrointestinal disease in humans. It can be spread through contaminated water.

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    Aerosols

    Microorganisms that are spread through the air, often in the form of small particles containing viable microorganisms. These particles can be inhaled and cause infections.

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    Influenza Virus

    A virus responsible for causing influenza, a respiratory illness spread through airborne droplets.

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    Cocci

    Spherical-shaped bacteria. They can exist individually, in pairs (diplococci), chains (streptococci), or clusters (staphylococci).

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    Bacilli

    Rod-shaped bacteria. They can occur singly or in chains.

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    Vibrio

    Curved or comma-shaped bacteria. They are shorter and thicker than spirochaetes.

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    Spirochaetes

    Spiral-shaped bacteria with a corkscrew-like appearance. They are much longer and thinner than vibrios.

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    Streptococcus

    A group of bacteria that cause various infections, including strep throat.

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    How big are bacteria?

    These bacteria are typically 0.2-2.0µm in diameter and 2-8µm in length. They are significantly smaller than red blood cells.

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    Microbial transfer/transmission routes

    This refers to the different ways bacteria can spread from one person or place to another, such as through direct contact, air, water, food, or vectors like insects.

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    What is the function of the bacterial cell membrane?

    The outermost layer of a bacterial cell, responsible for selective permeability, metabolism, ATP production, DNA replication, and protein secretion.

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    What is the function of the bacterial cell wall?

    A rigid structure surrounding the bacterial cell membrane, providing structural support and protection.

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    What is simple diffusion?

    A type of passive transport where molecules move across the membrane from an area of high concentration to an area of low concentration.

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    What is active transport?

    A type of active transport where molecules move across the membrane against their concentration gradient, requiring energy.

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    What is the structure of the Gram-positive cell wall?

    A thick layer of peptidoglycan, teichoic acids, and lipoteichoic acids that surrounds the cell membrane of Gram-positive bacteria.

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    What is the structure of the Gram-negative cell wall?

    A thin layer of peptidoglycan and an outer membrane containing lipopolysaccharides that surrounds the cell membrane of Gram-negative bacteria.

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    What is peptidoglycan?

    A repeating polymer of sugars and amino acids that forms the structural backbone of bacterial cell walls.

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    What is peptidoglycan synthesis?

    A process by which bacteria synthesize new peptidoglycan units and incorporate them into the existing cell wall, allowing the cell to grow.

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    What is Lipopolysaccharide (LPS)?

    A complex molecule that forms the outer layer of the cell wall in gram-negative bacteria. It is composed of three main parts: O-specific polysaccharide, core polysaccharide and Lipid A. The O-specific region is antigenic and Lipid A is responsible for the toxic effects of the bacteria.

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    What is the cell wall structure of gram-positive bacteria?

    A thick layer of peptidoglycan, along with teichoic acids, forms a rigid structure surrounding the cell membrane of gram-positive bacteria. This structure gives the cell its shape and protects it from osmotic pressure.

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    What is the cell wall structure of gram-negative bacteria?

    Gram-negative bacteria have a thinner layer of peptidoglycan compared to gram-positive bacteria. This peptidoglycan layer is sandwiched between two membranes, the inner cell membrane and the outer membrane, which contains lipopolysaccharide (LPS).

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    What is the periplasmic space in gram-negative bacteria?

    A space between the outer membrane and the inner cell membrane in gram-negative bacteria. This space is filled with enzymes and proteins that help the bacterium to break down food and transport nutrients.

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    What are Gram-positive bacteria?

    A group of bacteria that have a thick layer of peptidoglycan in their cell walls. They are generally more resistant to antibiotics and other antimicrobial agents than Gram-negative bacteria.

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    What are Gram-negative bacteria?

    A group of bacteria that have a thin layer of peptidoglycan in their cell walls, nestled between two membranes. They are often more susceptible to antibiotics than Gram-positive bacteria.

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    What are teichoic acids?

    A type of substance found in the cell walls of some bacteria, especially gram-positive bacteria. It is composed of sugars and phosphates. They contribute to the strength and rigidity of the cell wall, and help the bacteria to adhere to surfaces.

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    Name three common examples of gram-positive bacteria?

    Staphylococcus aureus, Bacillus cereus and Streptococcus pyogenes are all examples of common gram-positive bacteria that can cause infections.

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    Study Notes

    Introduction to Microbiology (BY451)

    • Microbiology is the study of a diverse range of organisms, including bacteria, archaea, viruses, protists, and akaryotes.
    • These organisms can be found in various habitats like soil, water, plants, and animal intestines.
    • Microorganisms are vital to society, playing a role in food production, vaccine development, and antibiotic production.
    • Antonie van Leeuwenhoek's 1683 description of microbes in tooth plaque was the first observation of bacteria.
    • His drawings depicted bacterial shapes like rods (bacilli), spheres (cocci), and corkscrew-shaped spirilla.

    Learning Outcomes

    • Students will comprehend the scope of microbiology.
    • Students will understand the definitions of eukaryote, prokaryote, and akaryote.
    • Students need to understand the meanings of mutualism, predation, nutrient cycling, and microbial transfer routes.
    • Understanding key aspects of bacterial cell biology is required.
    • The current lecture and the following one are strongly interconnected.

    What is Microbiology?

    • Microbiology encompasses the study of microorganisms, encompassing bacteria, archaea, viruses, protists, and akaryotes.
    • Microorganisms inhabit a vast array of environments, including soil, water, and within organisms.
    • Microorganisms' applications extend to diverse areas, including food production, vaccine development, and antibiotic production.

    Antonie van Leeuwenhoek

    • His 1683 observations of microbes in tooth plaque marked the initial recognition of bacteria.
    • His drawings showcased various bacterial shapes like rods, spheres, and spirals.

    Important Historical Names in Microbiology

    • Louis Pasteur promoted vaccination and advanced germ theory. He developed pasteurization.
    • Edward Jenner initiated the first vaccination programs in England.
    • Frederick Griffith discovered the phenomenon of bacterial transformation.
    • Alexander Fleming discovered penicillin.

    What is a Eukaryote?

    • Eukaryotes possess a cell nucleus with multiple chromosomes housed within a nuclear envelope and membrane-bound organelles (e.g. Golgi apparatus).
    • Eukaryotes include animals, plants, and fungi.
    • Animal cells are examples of eukaryotes.

    Example Eukaryote: Penicillium roqueforti

    • Used in blue cheese production.
    • A fungus that produces proteases and lipases, leading to the unique texture and flavor in cheese.
    • Related to Penicillium chrysogenum, which yields penicillin.

    What is a Prokaryote?

    • Prokaryotes are single-celled organisms belonging to the Bacteria or Archaea domains of life.
    • They possess a single chromosome but lack a nucleus and other membrane-bound organelles.
    • Bacteria and Archaea are similar in size and shape but differ greatly in their biological characteristics and adaptations to various environmental conditions.

    Example Prokaryote: Escherichia coli

    • Inhabits the intestines of humans and animals.
    • Can cause diarrhea and other severe diseases
    • Related to organisms such as Salmonella sp and Yersinia pestis.

    What is an Akaryote?

    • An akaryote is an organism devoid of a cell nucleus.
    • Examples include viruses and prions.
    • Akaryotes lack the capacity for independent replication, contrasting eukaryotes and prokaryotes.

    What is Mutualism?

    • Mutualism describes a close, persistent association between two populations where both benefit.
    • Rhizobium sp., a bacterium found in association with certain plant species, serves as an example.
    • Rhizobium sp. facilitates nitrogen fixation, benefiting both the bacteria (favorable environment within the plant) and the plant (increased nitrogen for growth).

    What is Predation?

    • Predation is characterized by one microorganism feeding on another.
    • Predatory microorganisms generally exhibit active motility, an ability to secrete compounds for degrading prey's cell contents, and other shared features
    • The outcome is the death of the prey, while the predator gains sustenance.

    Bacterial Predation

    • Certain bacteria practice predation, consuming other bacteria.
    • Predatory behaviors include prey location, attachment, secretion of hydrolytic enzymes, ingestion, and binary division.

    Key Learning Points

    • Organisms often exist in relationships, beneficial (e.g., nitrogen fixation) or harmful (e.g., predation) to each partner and to the environment.
    • Bacteria, viruses, and fungi can cause human diseases.
    • Human and animal association with microorganisms potentially leads to mutualistic interactions.

    Microorganisms (Thinking Task)

    • Consider diverse microorganisms that cause infectious diseases (e.g. influenza).
    • Recall microorganisms that participate in essential processes.

    Movement of Microorganisms

    • Microorganisms can travel between locations through natural factors like air and water currents.
    • Personal contact is also a means of spread, involving skin-to-skin touch, coughing/sneezing, and interactions involving bodily fluids.
    • Direct contact with animals (e.g., from consuming food, engaging in occupations, or due to lifestyle patterns) and human-related actions (manure, food transport, animal transport) contribute to microbial movement.

    Water Transmission

    • Water plays a key role in microbial transmission.
    • Vibrio cholerae, a bacterium causing gastrointestinal diseases, can contaminate water sources through fecal contamination
    • Drinking contaminated water is a route of infection.

    Person-to-Person Contact

    • Some microorganisms spread through skin-to-skin touch or aerosol inhalation (e.g. influenza virus, Staphylococcus aureus (a bacteria), Ebola virus).

    Staphylococcus aureus

    • Exists in the normal human microbiota
    • Resides on human mucous membranes and skin, and
    • Resistant to desiccation and persists on fomites.
    • Is an important hospital (nosocomial)-borne pathogen.

    Ebola

    • A filovirus with a zoonotic and human-to-human transmission.
    • Generally spread through direct contact.
    • Often fatal; the pathogenic process isn't fully understood.

    Contact with Animals

    • Microbes can transmit through animal contact, including food consumption, work-related activities, or lifestyle.

    Animal Husbandry

    • Associated with changes in the kept animal types and numbers.
    • Changes in husbandry (housing and care) methods.

    Escherichia coli (E. coli)

    • Transmitted via fecal contamination of soil, water, and surface waters.
    • Animal feces can contaminate soil and wash microbes into water sources like rivers and the sea.
    • Sewage leaks pose a risk of contamination

    Useful Processes

    • Microorganisms have beneficial applications, including vaccines (e.g. Polio, MMR), food production (e.g., bread, alcohol, cheese) and medicines (e.g, antibiotics, insulin).

    Examples of Vaccines and Sites of Vaccination

    • Vaccination routes include intramuscular (e.g. Influenza, Hepatitis A & B, Polio (Salk), sub-cutaneous (e.g MMR, Meningitis), and Oral (e.g Polio (Sabin).

    Microbially Derived Foods

    • Pediococcus species produce soy sauce.
    • Lactobacillus and Bifidobacterium create cheeses and yogurts, and are used in sauerkraut production.

    Summary & Advice

    • Microorganism cell biology and biochemistry allow us to understand transmission and harness their utility in areas such as food production.
    • Understand key transmission routes and example organisms
    • Remember to link concepts across lectures and labs.

    Beneficial Uses

    • Recycling elements
    • Sewage treatment
    • Bioremediation
    • Pest control (e.g. caterpillar control)
    • Biotechnology/genetic engineering
    • Chemical production
    • Food production (e.g. probiotics)
    • Normal microbiota of the body

    Leprosy (Hansen's Disease)

    • Caused by Mycobacterium leprae.

    Dental Decay

    • Dental plaque is a biofilm containing bacteria.
    • Dental decay is caused by the action of microorganisms on tooth enamel.

    Strep Throat

    • Streptococcus pyogenes is the causative agent for strep throat.

    Bacterial Shapes

    • Cocci are spherical cells. Examples include Staphylococcus aureus and various Streptococcal species.
    • Bacilli are oblong-shaped. Examples include Pseudomonas aeruginosa and Escherichia coli.
    • Vibrio are curved bacilli. Vibrio cholerae is an example.
    • Spirochaetes are corkscrew-shaped. Treponema pallidum is an example.

    Sizes of Bacteria

    • Red blood cell diameter is approximately 7.5 µm.
    • Bacteria range from 0.2-2.0 µm diameter and 2-8 µm in length
    • Viruses range from 20-14,000 nm (nanometers)

    Key Learning Points - Bacterial Structure

    • Bacteria exist in various shapes (cocci, bacilli, vibrios, spirochetes) and arrangements (pairs, chains, clusters).
    • Morphology and arrangement aid bacterial identification, sometimes down to the genus level.

    The Bacterial Cell (Key Features and Function)

    • Diagram showing key cell features and their function.

    Bacterial Packaging

    • Cell membrane and cell wall packaging.

    The Cell (Plasma) Membrane

    • A diagram depicting external environment; Glycoprotein; Glycolipid; lipid bilayer; integral protein; and peripheral protein; cytoplasm.

    Functions of the Cell Membrane

    • Selectively permeable barrier
    • Metabolism (enzymes)
    • Respiration and energy generation (ATP)
    • DNA replication
    • Cell wall synthesis
    • Protein secretion
    • Photosynthesis (some bacteria)

    Movement Across the Membrane

    • Movement of molecules using different transport mechanisms (passive transport, facilitated diffusion, osmosis active transport, group translocation)

    Comparison of Gram-positive and Gram-negative Cell Walls

    • Diagram contrasting Gram-positive and Gram-negative cell wall structures.

    The Gram-positive Cell Wall

    • Structure displaying the wall teichoic acid, lipoteichoic acid, periplasm, and plasma membrane.

    Peptidoglycan Structure

    • A diagram showing the structural components: N-acetylglucosamine, N-acetylmuramic acid, tetrapeptide chain, and peptide cross-bridge; β (1-4) glycosidic bond.

    Cross-linking of Peptidoglycan

    • Differences in the cross-linkage schemes of E. coli and Staphylococcus aureus

    Gram Positive Organisms

    • Names of common gram-positive organisms, including causative agents for various infections.

    The Gram-Negative Cell Wall

    • A diagram depicting the lipopolysaccharide; Protein (P); Lipoprotein (LP); Porin (POP); periplasmic space, plasma membrane, and peptidoglycan.

    Lipopolysaccharide (LPS)

    • Structure with O-specific polysaccharide, core polysaccharide and Lipid A.
    • Important role in toxicity associated with Gram negative cell walls.

    Examples of Gram-negative Organisms

    • Examples of common gram-negative bacteria including causative agents for various infections: Escherichia coli, Salmonella spp., and Pseudomonas aeruginosa.

    Key Learning Points - Gram Positive/Negative

    • Table summarizing the key differences in components of Gram-positive and Gram-negative bacterial cells.

    Suggested Reading

    • Brock Biology of Microorganisms, Chapter 2 (available from library or online).

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    Description

    Test your knowledge of microbiology with this quiz that covers various topics including the characteristics of cells, the role of microbes in cheese making, and key historical figures in the field. Answer questions about bacteria, their transmission, and the significance of mutualistic relationships. This quiz is designed for those interested in the foundational aspects of microbiological science.

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