Podcast
Questions and Answers
Which method is suitable for glassware instruments & paper-wrapped articles that are not spoiled by high temperature?
Which method is suitable for glassware instruments & paper-wrapped articles that are not spoiled by high temperature?
- Hot air oven at 140°C
- Steaming at 100°C
- Boiling at 100°C
- Red heat (correct)
What is the purpose of pasteurization in heat sterilization?
What is the purpose of pasteurization in heat sterilization?
- Used for inoculating wires and loops
- Kills vegetative and spore-forming bacteria
- Kills only vegetative cells (correct)
- Used for glasswares such as tubes, Petri dishes, flasks & glass pipettes
At what temperature does a hot air oven operate for 1 hour for effective sterilization?
At what temperature does a hot air oven operate for 1 hour for effective sterilization?
- 180°C
- 140°C (correct)
- 100°C
- 160°C
Which method is used with inoculating wires and loops for sterilization?
Which method is used with inoculating wires and loops for sterilization?
"Moist heat" is effective in killing which type of bacteria at lower temperatures compared to dry heat?
"Moist heat" is effective in killing which type of bacteria at lower temperatures compared to dry heat?
Flashcards
Red Heat
Red Heat
A sterilization method used on glassware instruments and paper-wrapped articles that can withstand high temperatures.
Pasteurization
Pasteurization
This method kills vegetative cells, which are the active, growing form of bacteria. It does not eliminate spores, which are dormant, resistant forms.
Hot Air Oven Temperature
Hot Air Oven Temperature
For 1 hour, a hot air oven works at 140°C to effectively sterilize materials.
Red Heat Sterilization (Inoculating Tools)
Red Heat Sterilization (Inoculating Tools)
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Effectiveness of Moist Heat
Effectiveness of Moist Heat
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Study Notes
Sterilization Methods
- Dry heat sterilization is suitable for glassware instruments and paper-wrapped articles that are not spoiled by high temperature.
Pasteurization
- The purpose of pasteurization in heat sterilization is to kill pathogens and extend shelf life.
Hot Air Oven Sterilization
- A hot air oven operates at a temperature suitable for effective sterilization for 1 hour.
Inoculating Wires and Loops Sterilization
- Flame sterilization is used with inoculating wires and loops for sterilization.
Moist Heat Sterilization
- Moist heat is effective in killing spores at lower temperatures compared to dry heat.
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