Microbiology Practical: Sterilization & Disinfection
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Questions and Answers

Which method is suitable for glassware instruments & paper-wrapped articles that are not spoiled by high temperature?

  • Hot air oven at 140°C
  • Steaming at 100°C
  • Boiling at 100°C
  • Red heat (correct)
  • What is the purpose of pasteurization in heat sterilization?

  • Used for inoculating wires and loops
  • Kills vegetative and spore-forming bacteria
  • Kills only vegetative cells (correct)
  • Used for glasswares such as tubes, Petri dishes, flasks & glass pipettes
  • At what temperature does a hot air oven operate for 1 hour for effective sterilization?

  • 180°C
  • 140°C (correct)
  • 100°C
  • 160°C
  • Which method is used with inoculating wires and loops for sterilization?

    <p>Red heat</p> Signup and view all the answers

    "Moist heat" is effective in killing which type of bacteria at lower temperatures compared to dry heat?

    <p>&quot;Both vegetative &amp; spore-forming bacteria&quot;</p> Signup and view all the answers

    Study Notes

    Sterilization Methods

    • Dry heat sterilization is suitable for glassware instruments and paper-wrapped articles that are not spoiled by high temperature.

    Pasteurization

    • The purpose of pasteurization in heat sterilization is to kill pathogens and extend shelf life.

    Hot Air Oven Sterilization

    • A hot air oven operates at a temperature suitable for effective sterilization for 1 hour.

    Inoculating Wires and Loops Sterilization

    • Flame sterilization is used with inoculating wires and loops for sterilization.

    Moist Heat Sterilization

    • Moist heat is effective in killing spores at lower temperatures compared to dry heat.

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    Description

    Test your knowledge on practical aspects of sterilization, disinfection, bacterial motility, staining, and bacterial culture media in microbiology. Explore safety in the laboratory, microscopy, and biochemical tests for bacterial identification.

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