Microbiology of Staphylococcus aureus

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What type of hemolysis is exhibited by Staphylococcus aureus?

  • α-hemolysis
  • γ-hemolysis
  • β-hemolysis (correct)
  • δ-hemolysis

Which of the following characteristics is NOT associated with Staphylococcus aureus?

  • Catalase-positive
  • Indole-positive (correct)
  • Coagulase-positive
  • Gram-positive cocci in clusters

What is the primary mechanism by which Staphylococcus aureus protein A contributes to its virulence?

  • Inhibiting complement activation and phagocytosis (correct)
  • Directly lysing red blood cells
  • Producing toxins that damage host cells
  • Promoting bacterial adherence to host tissues

What is the primary characteristic that differentiates Streptococcus pneumoniae from other viridans streptococci?

<p>Presence of a capsule (A)</p> Signup and view all the answers

Which of the following is a potential complication associated with Staphylococcus aureus infection?

<p>All of the above (D)</p> Signup and view all the answers

What is the significance of the superantigen TSST-1 produced by Staphylococcus aureus?

<p>It binds to MHC II and the T-cell receptor, causing polyclonal T-cell activation and cytokine release (D)</p> Signup and view all the answers

Which of the following bacterial species is NOT a β-hemolytic bacteria?

<p>Streptococcus pneumoniae (D)</p> Signup and view all the answers

Which of the following is a common site for Staphylococcus aureus colonization?

<p>Skin (C)</p> Signup and view all the answers

Which of the following virulence factors is NOT produced by Bordetella pertussis?

<p>Hemolysin (B)</p> Signup and view all the answers

Which of the following is a characteristic of Haemophilus influenzae?

<p>Requires both NAD+ and hematin for growth (D)</p> Signup and view all the answers

Which of the following is NOT a correct association?

<p><em>Legionella pneumophila</em> - cutaneous infections (A)</p> Signup and view all the answers

Which of the following bacterial species is associated with cystic fibrosis?

<p><em>Burkholderia cepacia</em> (A)</p> Signup and view all the answers

What is the primary mode of transmission for Brucella?

<p>Ingestion of contaminated animal products (D)</p> Signup and view all the answers

Which of the following bacterial species is best identified using a silver stain?

<p><em>Legionella pneumophila</em> (A)</p> Signup and view all the answers

Which of the following is NOT a characteristic of the paroxysmal stage of pertussis?

<p>High fever (D)</p> Signup and view all the answers

Which of the following treatment options is NOT commonly used for Brucella infections?

<p>Azithromycin (A)</p> Signup and view all the answers

What is the primary function of a transposon in bacterial genetics?

<p>To create plasmids with multiple drug resistance (A)</p> Signup and view all the answers

In which scenario is transposition particularly critical?

<p>When transferring genetic material across different species (D)</p> Signup and view all the answers

Which of the following statements about plasmids is accurate?

<p>Plasmids can contribute to multi-drug resistance in bacteria (B)</p> Signup and view all the answers

What is a transposon?

<p>A segment of DNA that can copy and excise itself (B)</p> Signup and view all the answers

What role does the target site play in the process of transposition?

<p>It is where the transposon integrates into the DNA (C)</p> Signup and view all the answers

Which bacteria are known for producing yellow pigments?

<p>Actinomyces israelii (A)</p> Signup and view all the answers

What is the primary reason for needing to autoclave spores of certain bacteria?

<p>Spores lack metabolic activity and are highly resistant to heat (D)</p> Signup and view all the answers

Which species is primarily associated with dental plaques and infective endocarditis?

<p>Viridans streptococci (B)</p> Signup and view all the answers

Which bacteria are notably associated with color, specifically blue-green pigmentation?

<p>P aeruginosa (D)</p> Signup and view all the answers

Which condition is primarily associated with P aeruginosa in patients with cystic fibrosis?

<p>Ventilator-associated pneumonia (C)</p> Signup and view all the answers

Which of the following bacteria form spores and are resistant to heat?

<p>C difficile (D)</p> Signup and view all the answers

What is the key characteristic of nontypeable H influenzae?

<p>Is unencapsulated (B)</p> Signup and view all the answers

Which bacterial species is responsible for gas gangrene?

<p>C perfringens (B)</p> Signup and view all the answers

What is the primary mode of transmission for Haemophilus influenzae?

<p>Through respiratory droplets (D)</p> Signup and view all the answers

Which of the following infections is NOT caused by Neisseria gonorrhoeae?

<p>Meningococcemia (A)</p> Signup and view all the answers

Which treatment is recommended for Neisseria gonorrhoeae if chlamydial coinfection is suspected?

<p>Ceftriaxone plus doxycycline (A)</p> Signup and view all the answers

What type of vaccine is used to prevent infections caused by Haemophilus influenzae type b?

<p>Conjugated vaccine (C)</p> Signup and view all the answers

Which culture medium is used to identify Haemophilus influenzae?

<p>Chocolate agar (C)</p> Signup and view all the answers

What happens to the recipient cell after it integrates new bacterial genes during high-frequency recombination?

<p>It remains F- but may have new bacterial genes. (B)</p> Signup and view all the answers

What is an important prophylactic treatment for contacts of someone infected with Neisseria meningitidis?

<p>Rifampin (C)</p> Signup and view all the answers

What is the result of transduction related to the 'packaging' error?

<p>Introduction of foreign DNA into a bacterial cell. (A)</p> Signup and view all the answers

Which of the following is a characteristic of non-typeable strains of Haemophilus influenzae?

<p>They are the most common cause of mucosal infections. (D)</p> Signup and view all the answers

What is the main function of IgA protease produced by Haemophilus influenzae?

<p>Destroys mucosal IgA (B)</p> Signup and view all the answers

Which cell type receives the leading part of a plasmid and chromosomal genes during recombination?

<p>F- cell (A)</p> Signup and view all the answers

Which statement is true about Hfr cells during the transfer process?

<p>Hfr cells can integrate chromosomal genes into recipient cells. (A)</p> Signup and view all the answers

What does the integration of new bacterial genes via high-frequency recombination imply for the Hfr cell?

<p>The Hfr cell provides gene combinations to F- cells. (A)</p> Signup and view all the answers

During the transfer to an F- cell, what component primarily influences the genetic makeup?

<p>Plasmid and chromosomal genes (A)</p> Signup and view all the answers

What remains unchanged in the recipient F- cell after the integration of new genes?

<p>The F factor status (A)</p> Signup and view all the answers

What type of genes can be integrated into an F- cell during high-frequency recombination?

<p>A mix of plasmid and chromosomal genes (D)</p> Signup and view all the answers

Flashcards

Transposon

A specialized segment of DNA that can copy itself and insert into another DNA molecule.

Transposition

A process in which a transposon copies itself and inserts into a new DNA molecule, often a plasmid or chromosome.

Plasmid

A small, circular piece of DNA that can replicate independently of the main bacterial chromosome.

Integration of genes

The process by which a transposon is incorporated into a bacterial chromosome or plasmid.

Signup and view all the flashcards

Tn1546

A type of transposon that carries genes for antibiotic resistance.

Signup and view all the flashcards

Pigment-producing bacteria

Bacteria that produce pigments, which are colored substances that give them a distinctive appearance.

Signup and view all the flashcards

Spore-forming bacteria

A type of bacteria that forms spores, which are resistant structures that allow them to survive harsh conditions.

Signup and view all the flashcards

What color pigment does Actinomyces israelii produce?

These bacteria produce yellow pigment.

Signup and view all the flashcards

What color pigment does Staphylococcus aureus produce?

These bacteria produce golden yellow pigment.

Signup and view all the flashcards

What color pigment does Pseudomonas aeruginosa produce?

These bacteria produce a blue-green pigment called pyocyanin and pyoverdin.

Signup and view all the flashcards

What color pigment does Serratia marcescens produce?

These bacteria produce a red pigment.

Signup and view all the flashcards

In vivo biofilm producing bacteria

These bacteria can form biofilms, which are communities of bacteria living together on a surface.

Signup and view all the flashcards

What infections are associated with Staphylococcus epidermidis?

These bacteria are known to cause catheter and prosthetic device infections.

Signup and view all the flashcards

F+ cell

A bacterial cell that contains a fertility factor plasmid, capable of transferring genetic material to a recipient cell.

Signup and view all the flashcards

F– cell

A bacterial cell that lacks the fertility factor plasmid, receiving genetic material from an F+ cell.

Signup and view all the flashcards

Hfr cell

A bacterial cell with an integrated F factor into its chromosomal DNA, capable of high-frequency recombination.

Signup and view all the flashcards

Conjugation

A process where a bacterial cell transfers genetic material to another cell through direct contact, by transferring a copy of its F factor.

Signup and view all the flashcards

Transduction

A process where a bacterial cell transfers its genetic material through the help of viruses.

Signup and view all the flashcards

High-frequency recombination

A process that occurs during conjugation, when a fragment of bacterial DNA breaks off the Hfr cell's chromosome and recombines with the recipient's DNA.

Signup and view all the flashcards

Recombinant F– cell

A bacterial cell that has received genetic material from an Hfr cell through conjugation.

Signup and view all the flashcards

Horizontal gene transfer

A process where genetic material is transferred between bacterial cells through direct contact or with the help of viruses.

Signup and view all the flashcards

β-hemolytic bacteria

Complete lysis of red blood cells (RBCs) resulting in a pale/clear area around the colony on blood agar.

Signup and view all the flashcards

α-hemolytic bacteria

Partial oxidation of hemoglobin producing a greenish or brownish color without clearing around the growth on blood agar.

Signup and view all the flashcards

Staphylococcus aureus

Staphylococcus aureus is a gram-positive, β-hemolytic, catalase-positive coccus that typically appears in clusters.

Signup and view all the flashcards

Protein A

A virulence factor that binds to the Fc portion of IgG, inhibiting complement activation and phagocytosis.

Signup and view all the flashcards

Toxic Shock Syndrome Toxin 1 (TSST-1)

A superantigen produced by Staphylococcus aureus that binds to MHC II and T-cell receptors, causing polyclonal T-cell activation and cytokine release.

Signup and view all the flashcards

Staphylococcal Toxic Shock Syndrome (TSS)

A serious illness caused by Staphylococcus aureus characterized by high fever, vomiting, diarrhea, rash, desquamation, shock, and end-organ failure.

Signup and view all the flashcards

Inflammatory disease

A common infection caused by Staphylococcus aureus leading to skin infections, organ abscesses, and pneumonia.

Signup and view all the flashcards

Catalase

Enzyme produced by Staphylococcus aureus that breaks down hydrogen peroxide, resulting in a positive reaction.

Signup and view all the flashcards

Neisseria gonorrhoeae

A bacterium that often causes infections in tight-knit groups like military barracks or college dorms.

Signup and view all the flashcards

Gonorrhea

A sexually transmitted infection caused by Neisseria gonorrhoeae.

Signup and view all the flashcards

Septic arthritis

A serious complication of gonorrhea that can affect joints.

Signup and view all the flashcards

Pelvic inflammatory disease (PID)

An inflammation of the pelvic organs, often caused by Neisseria gonorrhoeae.

Signup and view all the flashcards

Haemophilus influenzae

A bacterium that can cause a range of infections, from ear infections to meningitis.

Signup and view all the flashcards

Meningitis

A serious infection of the membranes surrounding the brain and spinal cord, caused by Haemophilus influenzae.

Signup and view all the flashcards

Nontypeable Haemophilus influenzae

A type of Haemophilus influenzae that is a common cause of ear infections.

Signup and view all the flashcards

Hib vaccine

A vaccine against Haemophilus influenzae type b, which helps protect against serious infections.

Signup and view all the flashcards

Epiglottitis

A severe infection that can cause a swelling in the back of the throat, seen as a "cherry red" color in children and as a "thumb sign" on an X-ray.

Signup and view all the flashcards

Burkholderia cepacia

A type of bacteria that grows aerobically (with oxygen), produces catalase enzyme (breaks down hydrogen peroxide), and is gram-negative rod.

Signup and view all the flashcards

Burkholderia cepacia Pneumonia

A potentially fatal lung infection caused by Burkholderia cepacia, which is particularly dangerous in patients with cystic fibrosis.

Signup and view all the flashcards

Bordetella pertussis

A gram-negative, aerobic coccobacillus (a tiny, oval-shaped bacterium) that causes whooping cough.

Signup and view all the flashcards

Undulant Fever

A type of fever that comes and goes in waves, characterized by chills, sweats, and joint pain, caused by Brucella bacteria.

Signup and view all the flashcards

Brucella

A gram-negative, aerobic coccobacillus (a tiny, oval-shaped bacterium) that causes brucellosis, a disease commonly transmitted through contaminated milk and dairy products.

Signup and view all the flashcards

Study Notes

High-Yield Principles in Microbiology

  • Microbiology questions frequently require two-step processes
  • First, identify the causative organism for a clinical presentation.
  • Then, provide an answer about that specific organism or relevant antimicrobials.
  • Organisms are presented in individual "profiles" and within their respective systems of infection.
  • Memorize laboratory characteristics for quick identification of microbes.

Bacterial Structures

  • Appendages (flagella, pili/fimbriae):

    • Flagella are proteins for motility.
    • Pili/fimbriae form during conjugation for adherence.
  • Specialized Structures (spores):

    • Spores are highly resistant to heat and chemicals, essential for survival. Core contains dipicolinic acid.
  • Cell Envelope

    • Capsule: prevents phagocytosis.
    • Slime Layer: mediates adherence, biofilm formation.
    • Outer Membrane: contains endotoxin, proteins (porins) for transport.
    • Periplasm: space between cytoplasmic and outer membrane in gram-positive bacteria.
    • Cell Wall: peptidoglycan for structural support, protects against osmotic pressure.
    • Cytoplasmic Membrane: phospholipid bilayer containing proteins for transport and other enzymatic functions.
    • Lipoteichoic Acids: found only in gram-positive bacterial cell walls.

Stains

  • Gram Stain: used to distinguish gram-positive and gram-negative bacteria based on their cell wall structure.
  • Giemsa Stain: stains glycogen and mucopolysaccharides, diagnosing conditions like Whipple's disease.
  • Periodic Acid-Schiff (PAS) Stain: used to stain glycoproteins and mucopolysaccharides
  • Ziehl-Neelsen Stain (Acid-fast stain): designed to identify acid-fast bacteria (e.g., mycobacteria) using carbol fuchsin.
  • India Ink Stain: for detecting Cryptococcus neoformans capsules.
  • Silver Stain: highlighting specific substances or structures like Helicobacter pylori.
  • Fluorescent Antibody Stain: used to identify many bacteria, viruses, fungi.

Special Culture Requirements

  • Specific media are required for different microbes to facilitate their growth
  • For example, H. influenzae needs factors V (NAD+) and X (hemating).
  • Different media contain varying factors to meet the needs specific micro-organisms.

Anaerobes

  • Anaerobes are normal microbiota in the GI tract but can be pathogenic elsewhere
  • They are sensitive to oxidative damage and lack catalase/superoxide dismutase.
  • Requires specific anaerobic conditions for growth.

Intracellular Bacteria

  • Intracellular bacteria like Rickettsia and Chlamydia live and replicate within host cells.
  • Use specific mechanisms to evade host immune responses.
  • These bacteria rely on host cellular components to survive and reproduce.

Encapsulated Bacteria

  • Encapsulated bacteria have a polysaccharide capsule serving as an antiphagocytic virulence factor.
  • The capsules prevent phagocytosis by immune cells.
  • Vaccination against encapsulated bacteria utilizes the polysaccharide antigen.

Urease-Positive Organisms

  • High urease activity plays a role in the development of kidney stones (eg, struvite).
  • This is due to the production of ammonia from urea, increasing pH.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

Related Documents

FA Microbiology PDF

More Like This

Use Quizgecko on...
Browser
Browser