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Questions and Answers
What type of hemolysis is exhibited by Staphylococcus aureus?
What type of hemolysis is exhibited by Staphylococcus aureus?
- α-hemolysis
- γ-hemolysis
- β-hemolysis (correct)
- δ-hemolysis
Which of the following characteristics is NOT associated with Staphylococcus aureus?
Which of the following characteristics is NOT associated with Staphylococcus aureus?
- Catalase-positive
- Indole-positive (correct)
- Coagulase-positive
- Gram-positive cocci in clusters
What is the primary mechanism by which Staphylococcus aureus protein A contributes to its virulence?
What is the primary mechanism by which Staphylococcus aureus protein A contributes to its virulence?
- Inhibiting complement activation and phagocytosis (correct)
- Directly lysing red blood cells
- Producing toxins that damage host cells
- Promoting bacterial adherence to host tissues
What is the primary characteristic that differentiates Streptococcus pneumoniae from other viridans streptococci?
What is the primary characteristic that differentiates Streptococcus pneumoniae from other viridans streptococci?
Which of the following is a potential complication associated with Staphylococcus aureus infection?
Which of the following is a potential complication associated with Staphylococcus aureus infection?
What is the significance of the superantigen TSST-1 produced by Staphylococcus aureus?
What is the significance of the superantigen TSST-1 produced by Staphylococcus aureus?
Which of the following bacterial species is NOT a β-hemolytic bacteria?
Which of the following bacterial species is NOT a β-hemolytic bacteria?
Which of the following is a common site for Staphylococcus aureus colonization?
Which of the following is a common site for Staphylococcus aureus colonization?
Which of the following virulence factors is NOT produced by Bordetella pertussis?
Which of the following virulence factors is NOT produced by Bordetella pertussis?
Which of the following is a characteristic of Haemophilus influenzae?
Which of the following is a characteristic of Haemophilus influenzae?
Which of the following is NOT a correct association?
Which of the following is NOT a correct association?
Which of the following bacterial species is associated with cystic fibrosis?
Which of the following bacterial species is associated with cystic fibrosis?
What is the primary mode of transmission for Brucella?
What is the primary mode of transmission for Brucella?
Which of the following bacterial species is best identified using a silver stain?
Which of the following bacterial species is best identified using a silver stain?
Which of the following is NOT a characteristic of the paroxysmal stage of pertussis?
Which of the following is NOT a characteristic of the paroxysmal stage of pertussis?
Which of the following treatment options is NOT commonly used for Brucella infections?
Which of the following treatment options is NOT commonly used for Brucella infections?
What is the primary function of a transposon in bacterial genetics?
What is the primary function of a transposon in bacterial genetics?
In which scenario is transposition particularly critical?
In which scenario is transposition particularly critical?
Which of the following statements about plasmids is accurate?
Which of the following statements about plasmids is accurate?
What is a transposon?
What is a transposon?
What role does the target site play in the process of transposition?
What role does the target site play in the process of transposition?
Which bacteria are known for producing yellow pigments?
Which bacteria are known for producing yellow pigments?
What is the primary reason for needing to autoclave spores of certain bacteria?
What is the primary reason for needing to autoclave spores of certain bacteria?
Which species is primarily associated with dental plaques and infective endocarditis?
Which species is primarily associated with dental plaques and infective endocarditis?
Which bacteria are notably associated with color, specifically blue-green pigmentation?
Which bacteria are notably associated with color, specifically blue-green pigmentation?
Which condition is primarily associated with P aeruginosa in patients with cystic fibrosis?
Which condition is primarily associated with P aeruginosa in patients with cystic fibrosis?
Which of the following bacteria form spores and are resistant to heat?
Which of the following bacteria form spores and are resistant to heat?
What is the key characteristic of nontypeable H influenzae?
What is the key characteristic of nontypeable H influenzae?
Which bacterial species is responsible for gas gangrene?
Which bacterial species is responsible for gas gangrene?
What is the primary mode of transmission for Haemophilus influenzae?
What is the primary mode of transmission for Haemophilus influenzae?
Which of the following infections is NOT caused by Neisseria gonorrhoeae?
Which of the following infections is NOT caused by Neisseria gonorrhoeae?
Which treatment is recommended for Neisseria gonorrhoeae if chlamydial coinfection is suspected?
Which treatment is recommended for Neisseria gonorrhoeae if chlamydial coinfection is suspected?
What type of vaccine is used to prevent infections caused by Haemophilus influenzae type b?
What type of vaccine is used to prevent infections caused by Haemophilus influenzae type b?
Which culture medium is used to identify Haemophilus influenzae?
Which culture medium is used to identify Haemophilus influenzae?
What happens to the recipient cell after it integrates new bacterial genes during high-frequency recombination?
What happens to the recipient cell after it integrates new bacterial genes during high-frequency recombination?
What is an important prophylactic treatment for contacts of someone infected with Neisseria meningitidis?
What is an important prophylactic treatment for contacts of someone infected with Neisseria meningitidis?
What is the result of transduction related to the 'packaging' error?
What is the result of transduction related to the 'packaging' error?
Which of the following is a characteristic of non-typeable strains of Haemophilus influenzae?
Which of the following is a characteristic of non-typeable strains of Haemophilus influenzae?
What is the main function of IgA protease produced by Haemophilus influenzae?
What is the main function of IgA protease produced by Haemophilus influenzae?
Which cell type receives the leading part of a plasmid and chromosomal genes during recombination?
Which cell type receives the leading part of a plasmid and chromosomal genes during recombination?
Which statement is true about Hfr cells during the transfer process?
Which statement is true about Hfr cells during the transfer process?
What does the integration of new bacterial genes via high-frequency recombination imply for the Hfr cell?
What does the integration of new bacterial genes via high-frequency recombination imply for the Hfr cell?
During the transfer to an F- cell, what component primarily influences the genetic makeup?
During the transfer to an F- cell, what component primarily influences the genetic makeup?
What remains unchanged in the recipient F- cell after the integration of new genes?
What remains unchanged in the recipient F- cell after the integration of new genes?
What type of genes can be integrated into an F- cell during high-frequency recombination?
What type of genes can be integrated into an F- cell during high-frequency recombination?
Flashcards
Transposon
Transposon
A specialized segment of DNA that can copy itself and insert into another DNA molecule.
Transposition
Transposition
A process in which a transposon copies itself and inserts into a new DNA molecule, often a plasmid or chromosome.
Plasmid
Plasmid
A small, circular piece of DNA that can replicate independently of the main bacterial chromosome.
Integration of genes
Integration of genes
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Tn1546
Tn1546
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Pigment-producing bacteria
Pigment-producing bacteria
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Spore-forming bacteria
Spore-forming bacteria
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What color pigment does Actinomyces israelii produce?
What color pigment does Actinomyces israelii produce?
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What color pigment does Staphylococcus aureus produce?
What color pigment does Staphylococcus aureus produce?
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What color pigment does Pseudomonas aeruginosa produce?
What color pigment does Pseudomonas aeruginosa produce?
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What color pigment does Serratia marcescens produce?
What color pigment does Serratia marcescens produce?
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In vivo biofilm producing bacteria
In vivo biofilm producing bacteria
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What infections are associated with Staphylococcus epidermidis?
What infections are associated with Staphylococcus epidermidis?
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F+ cell
F+ cell
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F– cell
F– cell
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Hfr cell
Hfr cell
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Conjugation
Conjugation
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Transduction
Transduction
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High-frequency recombination
High-frequency recombination
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Recombinant F– cell
Recombinant F– cell
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Horizontal gene transfer
Horizontal gene transfer
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β-hemolytic bacteria
β-hemolytic bacteria
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α-hemolytic bacteria
α-hemolytic bacteria
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Staphylococcus aureus
Staphylococcus aureus
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Protein A
Protein A
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Toxic Shock Syndrome Toxin 1 (TSST-1)
Toxic Shock Syndrome Toxin 1 (TSST-1)
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Staphylococcal Toxic Shock Syndrome (TSS)
Staphylococcal Toxic Shock Syndrome (TSS)
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Inflammatory disease
Inflammatory disease
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Catalase
Catalase
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Neisseria gonorrhoeae
Neisseria gonorrhoeae
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Gonorrhea
Gonorrhea
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Septic arthritis
Septic arthritis
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Pelvic inflammatory disease (PID)
Pelvic inflammatory disease (PID)
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Haemophilus influenzae
Haemophilus influenzae
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Meningitis
Meningitis
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Nontypeable Haemophilus influenzae
Nontypeable Haemophilus influenzae
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Hib vaccine
Hib vaccine
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Epiglottitis
Epiglottitis
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Burkholderia cepacia
Burkholderia cepacia
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Burkholderia cepacia Pneumonia
Burkholderia cepacia Pneumonia
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Bordetella pertussis
Bordetella pertussis
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Undulant Fever
Undulant Fever
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Brucella
Brucella
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Study Notes
High-Yield Principles in Microbiology
- Microbiology questions frequently require two-step processes
- First, identify the causative organism for a clinical presentation.
- Then, provide an answer about that specific organism or relevant antimicrobials.
- Organisms are presented in individual "profiles" and within their respective systems of infection.
- Memorize laboratory characteristics for quick identification of microbes.
Bacterial Structures
-
Appendages (flagella, pili/fimbriae):
- Flagella are proteins for motility.
- Pili/fimbriae form during conjugation for adherence.
-
Specialized Structures (spores):
- Spores are highly resistant to heat and chemicals, essential for survival. Core contains dipicolinic acid.
-
Cell Envelope
- Capsule: prevents phagocytosis.
- Slime Layer: mediates adherence, biofilm formation.
- Outer Membrane: contains endotoxin, proteins (porins) for transport.
- Periplasm: space between cytoplasmic and outer membrane in gram-positive bacteria.
- Cell Wall: peptidoglycan for structural support, protects against osmotic pressure.
- Cytoplasmic Membrane: phospholipid bilayer containing proteins for transport and other enzymatic functions.
- Lipoteichoic Acids: found only in gram-positive bacterial cell walls.
Stains
- Gram Stain: used to distinguish gram-positive and gram-negative bacteria based on their cell wall structure.
- Giemsa Stain: stains glycogen and mucopolysaccharides, diagnosing conditions like Whipple's disease.
- Periodic Acid-Schiff (PAS) Stain: used to stain glycoproteins and mucopolysaccharides
- Ziehl-Neelsen Stain (Acid-fast stain): designed to identify acid-fast bacteria (e.g., mycobacteria) using carbol fuchsin.
- India Ink Stain: for detecting Cryptococcus neoformans capsules.
- Silver Stain: highlighting specific substances or structures like Helicobacter pylori.
- Fluorescent Antibody Stain: used to identify many bacteria, viruses, fungi.
Special Culture Requirements
- Specific media are required for different microbes to facilitate their growth
- For example, H. influenzae needs factors V (NAD+) and X (hemating).
- Different media contain varying factors to meet the needs specific micro-organisms.
Anaerobes
- Anaerobes are normal microbiota in the GI tract but can be pathogenic elsewhere
- They are sensitive to oxidative damage and lack catalase/superoxide dismutase.
- Requires specific anaerobic conditions for growth.
Intracellular Bacteria
- Intracellular bacteria like Rickettsia and Chlamydia live and replicate within host cells.
- Use specific mechanisms to evade host immune responses.
- These bacteria rely on host cellular components to survive and reproduce.
Encapsulated Bacteria
- Encapsulated bacteria have a polysaccharide capsule serving as an antiphagocytic virulence factor.
- The capsules prevent phagocytosis by immune cells.
- Vaccination against encapsulated bacteria utilizes the polysaccharide antigen.
Urease-Positive Organisms
- High urease activity plays a role in the development of kidney stones (eg, struvite).
- This is due to the production of ammonia from urea, increasing pH.
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