Microbiology Lab Tests: Fermentation, Oxidation, and DNase Test
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Questions and Answers

What is the main purpose of the 'E' test mentioned in the text?

  • To quantify the number of bacteria in a sample
  • To assess the motility of bacteria
  • To identify the species of bacteria present in a sample
  • To determine the susceptibility of bacteria to antibiotics (correct)

    • What does a positive result in the 'E' test indicate?

  • Resistance to erythromycin
  • Increased motility of bacteria
  • Ellipse of growth inhibition (correct)
  • Appearance of a 'D pattern'

    • What is the main difference between BACTERIAL IDENTIFICATION CONVENTIONAL and ANALYTICAL methods mentioned in the text?

  • The presence of manual biochemical tests
  • The use of selective medium
  • The focus on motility and staining
  • The use of plastic strips and microtubes with biochemical substrates (correct)

    • What is the purpose of PEA (Phenyl Ethyl Alcohol) agar?

    <p>To identify gram positive cocci and other anaerobic gram negative rods</p> Signup and view all the answers

    In the thioglycollate enrichment broth, where does the growth typically occur for strict aerobes?

    <p>Towards the surface</p> Signup and view all the answers

    What is the purpose of the dilution method in susceptibility testing?

    <p>To determine minimum inhibitory concentration (MIC)</p> Signup and view all the answers

    What does MIC stand for in the context of susceptibility testing?

    <p>Minimum Inhibitory Concentration</p> Signup and view all the answers

    What is the purpose of the tube dilution method in susceptibility testing?

    <p>To determine minimum bactericidal concentration (MBC)</p> Signup and view all the answers

    In which method is serial dilution of antibiotics prepared on petri dishes?

    <p>Agar dilution method</p> Signup and view all the answers

    What is the purpose of the DNASE TEST/THERMONUCLEASE (+) test?

    <p>To detect the presence of deoxyribonuclease in an organism</p> Signup and view all the answers

    What is the result of a positive DNASE TEST/THERMONUCLEASE (+) test using methyl green dye?

    <p>Clear zone around the colonies</p> Signup and view all the answers

    Which of the following is a test for the identification of an organism?

    <p>VP and Nitrate (+)</p> Signup and view all the answers

    What is the definitive test for S. aureus?

    <p>COAGULASE (+)</p> Signup and view all the answers

    What is the result of a positive CEPHALOSPORINASE TEST using a Cefinase disk?

    <p>Pink to Red (change in color) within 60 minutes</p> Signup and view all the answers

    What are organisms sensitive to, according to the text?

    <p>Lysostaphin</p> Signup and view all the answers

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