Podcast
Questions and Answers
Which of the following media types is primarily used for the selective isolation of Gram-positive bacteria?
Which of the following media types is primarily used for the selective isolation of Gram-positive bacteria?
- Mannitol Salt Agar (MSA)
- Eosin Methylene Blue (EMB) agar
- Phenylethyl Alcohol Agar (PEA) (correct)
- Nutrient agar
What is the purpose of calibrating an ocular microscope?
What is the purpose of calibrating an ocular microscope?
- To improve the resolution of the microscope's lenses
- To ensure the microscope is properly sterilized
- To accurately measure the size of microorganisms (correct)
- To adjust the light intensity for optimal viewing
Which of the following best describes the purpose of the streak plate method?
Which of the following best describes the purpose of the streak plate method?
- To observe the motility of microorganisms in a liquid medium
- To determine the antibiotic resistance of a bacterial strain
- To quantify the number of bacteria in a sample
- To create a pure culture of a single type of microorganism (correct)
What is the primary purpose of using aseptic techniques in a microbiology laboratory?
What is the primary purpose of using aseptic techniques in a microbiology laboratory?
How does UV radiation inhibit microbial growth?
How does UV radiation inhibit microbial growth?
When observing a bacterial sample under a microscope, you note that the cells appear as chains of spherical cells. Which of the following terms best describes this arrangement?
When observing a bacterial sample under a microscope, you note that the cells appear as chains of spherical cells. Which of the following terms best describes this arrangement?
On Mannitol Salt Agar (MSA), what indicates that an organism can ferment mannitol?
On Mannitol Salt Agar (MSA), what indicates that an organism can ferment mannitol?
Which of the following is NOT a characteristic that can typically be observed when examining colony morphology?
Which of the following is NOT a characteristic that can typically be observed when examining colony morphology?
In broth culture, you observe a cloudy appearance throughout the tube. What is the correct term for this type of growth?
In broth culture, you observe a cloudy appearance throughout the tube. What is the correct term for this type of growth?
A simple stain uses only one stain to color bacterial cells. What information can be gathered from a simple stain?
A simple stain uses only one stain to color bacterial cells. What information can be gathered from a simple stain?
Flashcards
Nutrient Broth/Agar
Nutrient Broth/Agar
A medium used for cultivating microorganisms, containing complex ingredients that support the growth of a wide variety of bacteria.
Ubiquity of Microorganisms
Ubiquity of Microorganisms
Microorganisms are virtually everywhere; found in diverse environments, both living and non-living.
Colony Morphology
Colony Morphology
Macroscopic features of a bacterial colony including size, shape, elevation, margin, texture, and color.
Growth Patterns in Broth
Growth Patterns in Broth
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Aseptic Transfer
Aseptic Transfer
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Streak Plate Isolation
Streak Plate Isolation
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Light Microscope
Light Microscope
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Ocular Microscope Calibration
Ocular Microscope Calibration
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Eukaryotic Microbe Examination
Eukaryotic Microbe Examination
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Phenylethyl Alcohol Agar
Phenylethyl Alcohol Agar
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Study Notes
- A list of exercises has been completed.
- The completed exercises are:
- Nutrient broth and agar preparations
- Ubiquity of microorganisms
- Colony morphology
- Growth patterns in broth
- Common aseptic transfers and inoculation methods
- Streak plate methods of isolation
- Intro to light microscope
- Calibration of the ocular microscope
- Microscopic examination of eukaryotic microbes
- Phenylethyl alcohol agar
- Mannitol salt agar
- Eosin methylene blue
- UV radiation on growth
- Simple staining
- The following bacteria are being studied:
- Bacillus subtilis
- Staphylococcus aureus
- Staphylococcus epidermidis
- Lactobacillus lactis
- Micrococcus Luteus
- Alcaligenes faecalis
- Enterobacter aerogenes
- Escherichia coli
- Pseudomonas aeruginosa
- Serratia marcescens
- Proteus Vulgaris
- The characteristics being studied are:
- Colony morphology
- Colony color
- Broth growth
- Microscopic morphology
- Microscopic arrangement
- Dimensions
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