Microbiology Lab Techniques

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Questions and Answers

Which of the following media types is primarily used for the selective isolation of Gram-positive bacteria?

  • Mannitol Salt Agar (MSA)
  • Eosin Methylene Blue (EMB) agar
  • Phenylethyl Alcohol Agar (PEA) (correct)
  • Nutrient agar

What is the purpose of calibrating an ocular microscope?

  • To improve the resolution of the microscope's lenses
  • To ensure the microscope is properly sterilized
  • To accurately measure the size of microorganisms (correct)
  • To adjust the light intensity for optimal viewing

Which of the following best describes the purpose of the streak plate method?

  • To observe the motility of microorganisms in a liquid medium
  • To determine the antibiotic resistance of a bacterial strain
  • To quantify the number of bacteria in a sample
  • To create a pure culture of a single type of microorganism (correct)

What is the primary purpose of using aseptic techniques in a microbiology laboratory?

<p>To prevent contamination of cultures, oneself, and the environment. (D)</p> Signup and view all the answers

How does UV radiation inhibit microbial growth?

<p>By causing mutations in DNA, preventing proper replication (A)</p> Signup and view all the answers

When observing a bacterial sample under a microscope, you note that the cells appear as chains of spherical cells. Which of the following terms best describes this arrangement?

<p>Streptococci (C)</p> Signup and view all the answers

On Mannitol Salt Agar (MSA), what indicates that an organism can ferment mannitol?

<p>A color change in the agar from pink to yellow (B)</p> Signup and view all the answers

Which of the following is NOT a characteristic that can typically be observed when examining colony morphology?

<p>Cellular arrangement (D)</p> Signup and view all the answers

In broth culture, you observe a cloudy appearance throughout the tube. What is the correct term for this type of growth?

<p>Turbidity (D)</p> Signup and view all the answers

A simple stain uses only one stain to color bacterial cells. What information can be gathered from a simple stain?

<p>The morphology and arrangement of the cells (C)</p> Signup and view all the answers

Flashcards

Nutrient Broth/Agar

A medium used for cultivating microorganisms, containing complex ingredients that support the growth of a wide variety of bacteria.

Ubiquity of Microorganisms

Microorganisms are virtually everywhere; found in diverse environments, both living and non-living.

Colony Morphology

Macroscopic features of a bacterial colony including size, shape, elevation, margin, texture, and color.

Growth Patterns in Broth

Patterns of microbial growth in a liquid medium, such as turbidity, sediment, pellicle, or flocculence.

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Aseptic Transfer

Techniques, such as using sterilized equipment, to prevent contamination when transferring cultures.

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Streak Plate Isolation

A method to obtain isolated colonies by diluting a bacterial sample over an agar plate surface.

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Light Microscope

An optical instrument that uses visible light and a system of lenses to magnify small objects or structures.

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Ocular Microscope Calibration

Process of determining the relationship between divisions on the ocular micrometer and the stage micrometer.

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Eukaryotic Microbe Examination

Observing features (cell shape, structures) of microorganisms like fungi and protozoa under a microscope.

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Phenylethyl Alcohol Agar

Selective medium that inhibits Gram-negative organisms, allowing Gram-positive bacteria to grow.

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Study Notes

  • A list of exercises has been completed.
  • The completed exercises are:
  • Nutrient broth and agar preparations
  • Ubiquity of microorganisms
  • Colony morphology
  • Growth patterns in broth
  • Common aseptic transfers and inoculation methods
  • Streak plate methods of isolation
  • Intro to light microscope
  • Calibration of the ocular microscope
  • Microscopic examination of eukaryotic microbes
  • Phenylethyl alcohol agar
  • Mannitol salt agar
  • Eosin methylene blue
  • UV radiation on growth
  • Simple staining
  • The following bacteria are being studied:
  • Bacillus subtilis
  • Staphylococcus aureus
  • Staphylococcus epidermidis
  • Lactobacillus lactis
  • Micrococcus Luteus
  • Alcaligenes faecalis
  • Enterobacter aerogenes
  • Escherichia coli
  • Pseudomonas aeruginosa
  • Serratia marcescens
  • Proteus Vulgaris
  • The characteristics being studied are:
  • Colony morphology
  • Colony color
  • Broth growth
  • Microscopic morphology
  • Microscopic arrangement
  • Dimensions

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