Bacterial Identification and Hemolysis Types

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Questions and Answers

What is the Gram stain characteristic of Clostridium species?

  • Gram-negative
  • Gram-positive (correct)
  • Gram-variable
  • Gram-inconclusive

Which test is performed to differentiate Staphylococcus aureus from Staphylococcus epidermidis?

  • Coagulase test (correct)
  • Catalase test
  • CAMP test
  • Optochin test

What does MRSA stand for?

  • Macrolide-resistant Staphylococcus aureus
  • Meningitis-resistant Streptococcus aureus
  • Multidrug-resistant Staphylococcus aureus
  • Methicillin-resistant Staphylococcus aureus (correct)

Which organism is responsible for causing diamond skin disease in pigs?

<p>Erysipelothrix rhusiopathiae (C)</p> Signup and view all the answers

What type of hemolysis is characterized by clear zones around colonies?

<p>Beta hemolysis (D)</p> Signup and view all the answers

Which bacteria are known to thrive in environments without oxygen?

<p>Obligate anaerobes (D)</p> Signup and view all the answers

Which test is used to confirm Streptococcus pneumoniae?

<p>Optochin test (D)</p> Signup and view all the answers

What serious condition is primarily caused by Streptococcus agalactiae?

<p>Mastitis (A)</p> Signup and view all the answers

What pigment is produced by certain strains of Pseudomonas aeruginosa?

<p>Bluish-green pigment (D)</p> Signup and view all the answers

Which organism is known to cause circling disease in ruminants?

<p>Encephalitis (B)</p> Signup and view all the answers

What is the cause of limberneck in waterfowl?

<p>Clostridium botulinum (D)</p> Signup and view all the answers

A common bacterial cause of pulmonary abscesses in foals

<p>Rhodococcus equi (A)</p> Signup and view all the answers

The cause of anthrax is

<p>Bacillus anthracis (B)</p> Signup and view all the answers

Flashcards

Catalase test

A test used to differentiate Staphylococcus and Streptococcus bacteria based on their ability to produce the enzyme catalase.

Alpha hemolysis

A type of bacterial hemolysis characterized by a partial breakdown of red blood cells, resulting in a greenish discoloration around bacterial colonies on blood agar.

Beta hemolysis

A type of bacterial hemolysis characterized by a complete breakdown of red blood cells, resulting in a clear zone around bacterial colonies on blood agar.

Optochin test

A test that differentiates between Streptococcus pneumoniae and other alpha-hemolytic streptococci based on their sensitivity to the antibiotic optochin.

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Streptococcus equi infection

A bacterial infection that affects the upper respiratory tract and lymph nodes in horses, causing highly contagious symptoms.

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Coagulase test

A test that differentiates between Staphylococcus aureus and other Staphylococcus species based on their ability to produce the enzyme coagulase.

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Clostridium botulinum

The cause of limberneck in waterfowl, a neurological disease characterized by muscle weakness and paralysis.

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Erysipelothrix rhusiopathia

A bacterium responsible for diamond skin disease in pigs, causing skin lesions and systemic symptoms.

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Bacillus anthracis

The cause of anthrax, a serious infectious disease affecting various animals and humans.

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Rhodococcus equi

A bacterium commonly found in foals, causing pulmonary abscesses and respiratory problems

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Study Notes

Hemolysis Types

  • Three types of hemolysis exist: Alpha, Beta, and Gamma.

Bacterial Identification Tests

  • Catalase test: Differentiates Staphylococcus and Streptococcus species.
  • Optochin test: Identifies S. pneumoniae.
  • CAMP test: Used for S. agalactiae.
  • Coagulase test: Identifies Staphylococcus aureus. Incubation in rabbit plasma is needed. Yellow color change indicates S. aureus, while no change indicates S. epidermidis.

Bacterial Species and Diseases

  • Pseudomonas aeruginosa: Some strains produce a bluish-green pigment.
  • Streptococcus equi: Causes a highly contagious disease affecting the upper respiratory tract and lymph nodes.
  • Streptococcus agalactiae: Causes mastitis.
  • Clostridium tetani: Causes tetanus.
  • Clostridium botulinum: Causes limberneck in waterfowl.
  • Erysipelothrix rhusiopathiae: Causes diamond skin disease in pigs.
  • Bacillus anthracis: Causes anthrax. Anthrax bacteria survive by forming endospores.
  • Rhodococcus equi: A common bacterial cause of pulmonary abscesses in foals.
  • MRSA: Methicillin-resistant Staphylococcus aureus.

Bacterial Growth Media

  • Staphylococcus spp.: Grow on Blood Agar, Mannitol Salt Agar (MSA), and Nutrient Agar. Do not grow on MacConkey Agar.
  • Streptococcus spp.: Grow on Blood Agar and Chocolate Agar. Do not grow on MacConkey Agar.

Clostridium spp. Characteristics

  • Gram stain: Gram-positive.
  • Oxygen requirements: Obligate anaerobes (thrive in environments without oxygen).
  • Survival tactics: Form endospores.

Other Diseases

  • Encephalitis: Cause of circling disease in ruminants.

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