Questions and Answers
All of the following are methods that can be used to isolate bacteria from a mixed culture EXCEPT?
Which organelle is involved in the packaging of proteins for secretion?
The internationally recognized reference for bacterial classification and identification is called Bergey's Manual.
True
Which carbohydrate is the primary or preferred starting compound for glycolysis and the fermentation pathway?
Signup and view all the answers
The term aerobic, anaerobic, and microaerophilic are used to categorize microorganisms based on their ______ requirements.
Signup and view all the answers
All of the following body sites normally have large numbers of resident microbiota EXCEPT?
Signup and view all the answers
Which of the following items would be disposed of into the sharps container?
Signup and view all the answers
Most human pathogens are classified as thermophiles.
Signup and view all the answers
If an organism produces stable acids as an end-product of glucose fermentation, the methyl red test (MR test) will be red, indicating a positive result.
Signup and view all the answers
Which statement correctly describes the level of contamination on different objects?
Signup and view all the answers
How is a glass culture tube handled during aseptic transfers?
Signup and view all the answers
When is it crucial to wear protective eyewear when working in the lab?
Signup and view all the answers
Which of the following sequences would result from the replication of the following DNA sequence: ATTGCTGGA?
Signup and view all the answers
Microorganisms can be described as being ubiquitous which means that they:
Signup and view all the answers
Which of the following diseases are caused by acid-fast bacterium:
Signup and view all the answers
Which of the following is a chemical means of controlling microbial growth?
Signup and view all the answers
Catalase breaks hydrogen peroxide down in water and carbon dioxide.
Signup and view all the answers
How is a respirometer used to measure fermentation in the Cellular Respiration experiment?
Signup and view all the answers
Gram negative cells appear purple after Gram staining whereas gram positive cells appear pink.
Signup and view all the answers
The cloudy growth seen in a broth culture is referred to as:
Signup and view all the answers
Which test uses a medium that is both selective and differential for members of the genus Staphylococcus?
Signup and view all the answers
In addition to linking together nucleotides to synthesize a daughter strand, DNA polymerase also ______.
Signup and view all the answers
An antibiotic which interferes with the activity of ribosomes would have the most effect on which of the following?
Signup and view all the answers
Human skin provides an environment with NaCl levels slightly above that of most bacterial cells. Which organism would most likely be found on the skin?
Signup and view all the answers
All of the following are found in most prokaryotic cells EXCEPT:
Signup and view all the answers
The smallest living units of the human body are __________.
Signup and view all the answers
An organism has been shown to produce hydrogen sulfide and indole in its metabolism. These are examples of _____________ characteristics that can be helpful in the identification of the microorganism.
Signup and view all the answers
All of the following are common to all cells at some point in their development EXCEPT:
Signup and view all the answers
The purpose of heat fixation is to _______.
Signup and view all the answers
Which of the following is true of a bacterial species that produces dark colonies when grown on MacConkey agar?
Signup and view all the answers
When first viewing a specimen using the brightfield microscope, it is recommended that you begin viewing the specimen with which objective lens?
Signup and view all the answers
In living organisms, information for making proteins flows from _______.
Signup and view all the answers
An antimicrobial, such as isoniazid, that is only effective against mycobacterial species would be referred to as a __________ drug.
Signup and view all the answers
The ability to distinguish fine detail or discriminate between two very closely spaced objects is called:
Signup and view all the answers
Which type of reaction(s) requires oxygen as a substrate?
Signup and view all the answers
Phenol red carbohydrate broths with Durham tubes can detect all of the following EXCEPT:
Signup and view all the answers
The monomers that make up DNA molecules are called _______.
Signup and view all the answers
The process of forming mRNA from the template strand of DNA is called _____________.
Signup and view all the answers
Immersion oil must only be used with which objective lens?
Signup and view all the answers
The sources of all antimicrobial drugs are fungi and other microorganisms.
Signup and view all the answers
In the Gram stain, the chemical that fixes dye in or on cells by forming an insoluble compound is referred to as the _______________.
Signup and view all the answers
A pure culture contains:
Signup and view all the answers
All of the following are true of an enzyme-catalyzed reaction EXCEPT?
Signup and view all the answers
Before attempting to identify an unknown bacterial species, the organism must be isolated in a pure culture.
Signup and view all the answers
When a large zone of no growth is found around an antibiotic disc being used for identification purposes, this generally means that the microbe is ______ to the antibiotic.
Signup and view all the answers
Stains are commonly used for all of the following EXCEPT:
Signup and view all the answers
All of the following organisms are expected to grow within an anaerobe jar EXCEPT:
Signup and view all the answers
Eukaryotes have membrane-bound organelles.
Signup and view all the answers
A hypertonic solution is prepared by the addition of sucrose. Which of the following would happen to bacteria placed in this solution?
Signup and view all the answers
Which of the following processes removes or destroys endospores?
Signup and view all the answers
Study Notes
Bacterial Isolation and Identification
- Various methods to isolate bacteria include streak plating, loop dilution, and serial dilution followed by spread plating; Gram staining is not an isolation method.
- Bergey's Manual is the authoritative reference for bacterial classification and identification.
Cellular Components and Functionality
- The Golgi apparatus is responsible for packaging proteins for secretion.
- Cell nucleus is absent in prokaryotic cells, while cell membrane, cytoplasm, and cell wall are common.
Microbial Classification
- Microorganisms are categorized as aerobic, anaerobic, or microaerophilic based on oxygen requirements.
- Most human pathogens are mesophiles, not thermophiles.
Laboratory Practices
- Used microscope slides should be disposed of in sharps containers.
- Protective eyewear is essential when handling hazardous chemicals in the lab.
Genetics and Metabolism
- The primary carbohydrate for glycolysis and fermentation is glucose.
- DNA polymerase "proofreads" nucleotides when synthesizing the daughter strand.
- Fermentation assessment can utilize a respirometer to measure gas bubble size produced.
Microbial Growth and Behavior
- Microorganisms impacted by sodium chloride (NaCl) on human skin include halotolerant organisms.
- Lactose fermenters produce dark colonies on MacConkey agar, contrasting with other species.
Microscopy
- The initial viewing of specimens under a brightfield microscope should start with a 4X scanning objective.
Antimicrobial Agents
- Antibiotics targeting ribosomes affect protein synthesis. An antibiotic specific to mycobacterial species is considered a narrow-spectrum drug.
- Sterilization is the process that effectively removes or destroys endospores.
Staining and Identification Techniques
- Heat fixation in microscopy adheres microorganisms to slides and prevents contamination during staining.
- Stains assist in disease diagnosis, pathogen identification, and organism classification, but not disease treatment.
Microbial Characteristics
- Ubiquitous microorganisms are found on nearly every surface.
- Physiological characteristics such as hydrogen sulfide and indole production assist in microorganism identification.
Other Key Concepts
- The process of forming mRNA from DNA is known as transcription.
- Resolution refers to distinguishing fine details between closely spaced objects in microscopy.
- A hypertonic sucrose solution leads to plasmolysis in bacteria due to osmotic pressure.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
Test your knowledge with this set of flashcards focusing on key concepts from microbiology lab work. The quiz covers methods for isolating bacteria and organelles involved in protein packaging, among other topics. Perfect for students looking to reinforce their understanding of microbiology.