Microbiology Lab Study Questions

Questions and Answers

All of the following are methods that can be used to isolate bacteria from a mixed culture EXCEPT?

Gram staining (correct)

Loop dilution

Streak plating

Serial dilution followed by spread plating

Which organelle is involved in the packaging of proteins for secretion?

Rough endoplasmic reticulum

Mitochondrion

Golgi apparatus (correct)

Nucleus

The internationally recognized reference for bacterial classification and identification is called Bergey's Manual.

True

Which carbohydrate is the primary or preferred starting compound for glycolysis and the fermentation pathway?

Glucose Signup and view all the answers

The term aerobic, anaerobic, and microaerophilic are used to categorize microorganisms based on their ______ requirements.

oxygen Signup and view all the answers

All of the following body sites normally have large numbers of resident microbiota EXCEPT?

Blood Signup and view all the answers

Which of the following items would be disposed of into the sharps container?

Used microscope slides Signup and view all the answers

Most human pathogens are classified as thermophiles.

False Signup and view all the answers

If an organism produces stable acids as an end-product of glucose fermentation, the methyl red test (MR test) will be red, indicating a positive result.

True Signup and view all the answers

Which statement correctly describes the level of contamination on different objects?

Different objects will vary in their level of contamination. Signup and view all the answers

How is a glass culture tube handled during aseptic transfers?

The mouth is passed through the flame right when opened and just before closing Signup and view all the answers

When is it crucial to wear protective eyewear when working in the lab?

When working with hazardous chemicals Signup and view all the answers

Which of the following sequences would result from the replication of the following DNA sequence: ATTGCTGGA?

TAACGACCT Signup and view all the answers

Microorganisms can be described as being ubiquitous which means that they:

Are present on nearly every surface Signup and view all the answers

Which of the following diseases are caused by acid-fast bacterium:

Tuberculosis (TB) Signup and view all the answers

Which of the following is a chemical means of controlling microbial growth?

Disinfectant Signup and view all the answers

Catalase breaks hydrogen peroxide down in water and carbon dioxide.

False Signup and view all the answers

How is a respirometer used to measure fermentation in the Cellular Respiration experiment?

By the size of the gas bubble produced Signup and view all the answers

Gram negative cells appear purple after Gram staining whereas gram positive cells appear pink.

False Signup and view all the answers

The cloudy growth seen in a broth culture is referred to as:

Turbidity Signup and view all the answers

Which test uses a medium that is both selective and differential for members of the genus Staphylococcus?

Mannitol Salt agar Signup and view all the answers

In addition to linking together nucleotides to synthesize a daughter strand, DNA polymerase also ______.

Proofreads the order of nucleotides Signup and view all the answers

An antibiotic which interferes with the activity of ribosomes would have the most effect on which of the following?

Protein synthesis Signup and view all the answers

Human skin provides an environment with NaCl levels slightly above that of most bacterial cells. Which organism would most likely be found on the skin?

A halotolerant organism Signup and view all the answers

All of the following are found in most prokaryotic cells EXCEPT:

Nucleus Signup and view all the answers

The smallest living units of the human body are __________.

cells Signup and view all the answers

An organism has been shown to produce hydrogen sulfide and indole in its metabolism. These are examples of _____________ characteristics that can be helpful in the identification of the microorganism.

physiological Signup and view all the answers

All of the following are common to all cells at some point in their development EXCEPT:

The cell wall Signup and view all the answers

The purpose of heat fixation is to _______.

Inactivate and adhere microorganisms placed on the slide Signup and view all the answers

Which of the following is true of a bacterial species that produces dark colonies when grown on MacConkey agar?

They are lactose fermenters Signup and view all the answers

When first viewing a specimen using the brightfield microscope, it is recommended that you begin viewing the specimen with which objective lens?

4X (scanning objective) Signup and view all the answers

In living organisms, information for making proteins flows from _______.

DNA to RNA to protein Signup and view all the answers

An antimicrobial, such as isoniazid, that is only effective against mycobacterial species would be referred to as a __________ drug.

Narrow spectrum Signup and view all the answers

The ability to distinguish fine detail or discriminate between two very closely spaced objects is called:

Resolution Signup and view all the answers

Which type of reaction(s) requires oxygen as a substrate?

Aerobic Signup and view all the answers

Phenol red carbohydrate broths with Durham tubes can detect all of the following EXCEPT:

Nitrate reduction Signup and view all the answers

The monomers that make up DNA molecules are called _______.

nucleotides Signup and view all the answers

The process of forming mRNA from the template strand of DNA is called _____________.

Transcription Signup and view all the answers

Immersion oil must only be used with which objective lens?

100x Signup and view all the answers

The sources of all antimicrobial drugs are fungi and other microorganisms.

False Signup and view all the answers

In the Gram stain, the chemical that fixes dye in or on cells by forming an insoluble compound is referred to as the _______________.

Mordant Signup and view all the answers

A pure culture contains:

One type of microorganism Signup and view all the answers

All of the following are true of an enzyme-catalyzed reaction EXCEPT?

Every time a substrate is converted to a product, a bit of the enzyme is used up Signup and view all the answers

Before attempting to identify an unknown bacterial species, the organism must be isolated in a pure culture.

True Signup and view all the answers

When a large zone of no growth is found around an antibiotic disc being used for identification purposes, this generally means that the microbe is ______ to the antibiotic.

Sensitive Signup and view all the answers

Stains are commonly used for all of the following EXCEPT:

The treatment of disease Signup and view all the answers

All of the following organisms are expected to grow within an anaerobe jar EXCEPT:

Obligate aerobe Signup and view all the answers

Eukaryotes have membrane-bound organelles.

True Signup and view all the answers

A hypertonic solution is prepared by the addition of sucrose. Which of the following would happen to bacteria placed in this solution?

The bacterial cells will undergo plasmolysis Signup and view all the answers

Which of the following processes removes or destroys endospores?

Sterilization Signup and view all the answers

Study Notes

Bacterial Isolation and Identification

Various methods to isolate bacteria include streak plating, loop dilution, and serial dilution followed by spread plating; Gram staining is not an isolation method.
Bergey's Manual is the authoritative reference for bacterial classification and identification.

Cellular Components and Functionality

The Golgi apparatus is responsible for packaging proteins for secretion.
Cell nucleus is absent in prokaryotic cells, while cell membrane, cytoplasm, and cell wall are common.

Microbial Classification

Microorganisms are categorized as aerobic, anaerobic, or microaerophilic based on oxygen requirements.
Most human pathogens are mesophiles, not thermophiles.

Laboratory Practices

Used microscope slides should be disposed of in sharps containers.
Protective eyewear is essential when handling hazardous chemicals in the lab.

Genetics and Metabolism

The primary carbohydrate for glycolysis and fermentation is glucose.
DNA polymerase "proofreads" nucleotides when synthesizing the daughter strand.
Fermentation assessment can utilize a respirometer to measure gas bubble size produced.

Microbial Growth and Behavior

Microorganisms impacted by sodium chloride (NaCl) on human skin include halotolerant organisms.
Lactose fermenters produce dark colonies on MacConkey agar, contrasting with other species.

Microscopy

The initial viewing of specimens under a brightfield microscope should start with a 4X scanning objective.

Antimicrobial Agents

Antibiotics targeting ribosomes affect protein synthesis. An antibiotic specific to mycobacterial species is considered a narrow-spectrum drug.
Sterilization is the process that effectively removes or destroys endospores.

Staining and Identification Techniques

Heat fixation in microscopy adheres microorganisms to slides and prevents contamination during staining.
Stains assist in disease diagnosis, pathogen identification, and organism classification, but not disease treatment.

Microbial Characteristics

Ubiquitous microorganisms are found on nearly every surface.
Physiological characteristics such as hydrogen sulfide and indole production assist in microorganism identification.

Other Key Concepts

The process of forming mRNA from DNA is known as transcription.
Resolution refers to distinguishing fine details between closely spaced objects in microscopy.
A hypertonic sucrose solution leads to plasmolysis in bacteria due to osmotic pressure.

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Test your knowledge with this set of flashcards focusing on key concepts from microbiology lab work. The quiz covers methods for isolating bacteria and organelles involved in protein packaging, among other topics. Perfect for students looking to reinforce their understanding of microbiology.

Microbiology Lab Study Questions

Questions and Answers

All of the following are methods that can be used to isolate bacteria from a mixed culture EXCEPT?

Which organelle is involved in the packaging of proteins for secretion?

The internationally recognized reference for bacterial classification and identification is called Bergey's Manual.

Which carbohydrate is the primary or preferred starting compound for glycolysis and the fermentation pathway?

The term aerobic, anaerobic, and microaerophilic are used to categorize microorganisms based on their ______ requirements.

All of the following body sites normally have large numbers of resident microbiota EXCEPT?

Which of the following items would be disposed of into the sharps container?

Most human pathogens are classified as thermophiles.

If an organism produces stable acids as an end-product of glucose fermentation, the methyl red test (MR test) will be red, indicating a positive result.

Which statement correctly describes the level of contamination on different objects?

How is a glass culture tube handled during aseptic transfers?

When is it crucial to wear protective eyewear when working in the lab?

Which of the following sequences would result from the replication of the following DNA sequence: ATTGCTGGA?

Microorganisms can be described as being ubiquitous which means that they:

Which of the following diseases are caused by acid-fast bacterium:

Which of the following is a chemical means of controlling microbial growth?

Catalase breaks hydrogen peroxide down in water and carbon dioxide.

How is a respirometer used to measure fermentation in the Cellular Respiration experiment?

Gram negative cells appear purple after Gram staining whereas gram positive cells appear pink.

The cloudy growth seen in a broth culture is referred to as:

Which test uses a medium that is both selective and differential for members of the genus Staphylococcus?

In addition to linking together nucleotides to synthesize a daughter strand, DNA polymerase also ______.

An antibiotic which interferes with the activity of ribosomes would have the most effect on which of the following?

Human skin provides an environment with NaCl levels slightly above that of most bacterial cells. Which organism would most likely be found on the skin?

All of the following are found in most prokaryotic cells EXCEPT:

The smallest living units of the human body are __________.

An organism has been shown to produce hydrogen sulfide and indole in its metabolism. These are examples of _____________ characteristics that can be helpful in the identification of the microorganism.

All of the following are common to all cells at some point in their development EXCEPT:

The purpose of heat fixation is to _______.

Which of the following is true of a bacterial species that produces dark colonies when grown on MacConkey agar?

When first viewing a specimen using the brightfield microscope, it is recommended that you begin viewing the specimen with which objective lens?

In living organisms, information for making proteins flows from _______.

An antimicrobial, such as isoniazid, that is only effective against mycobacterial species would be referred to as a __________ drug.

The ability to distinguish fine detail or discriminate between two very closely spaced objects is called:

Which type of reaction(s) requires oxygen as a substrate?

Phenol red carbohydrate broths with Durham tubes can detect all of the following EXCEPT:

The monomers that make up DNA molecules are called _______.

The process of forming mRNA from the template strand of DNA is called _____________.

Immersion oil must only be used with which objective lens?

The sources of all antimicrobial drugs are fungi and other microorganisms.

In the Gram stain, the chemical that fixes dye in or on cells by forming an insoluble compound is referred to as the _______________.

A pure culture contains:

All of the following are true of an enzyme-catalyzed reaction EXCEPT?

Before attempting to identify an unknown bacterial species, the organism must be isolated in a pure culture.

When a large zone of no growth is found around an antibiotic disc being used for identification purposes, this generally means that the microbe is ______ to the antibiotic.

Stains are commonly used for all of the following EXCEPT:

All of the following organisms are expected to grow within an anaerobe jar EXCEPT:

Eukaryotes have membrane-bound organelles.

A hypertonic solution is prepared by the addition of sucrose. Which of the following would happen to bacteria placed in this solution?

Which of the following processes removes or destroys endospores?

Study Notes

Bacterial Isolation and Identification

Cellular Components and Functionality

Microbial Classification

Laboratory Practices

Genetics and Metabolism

Microbial Growth and Behavior

Microscopy

Antimicrobial Agents

Staining and Identification Techniques

Microbial Characteristics

Other Key Concepts

Studying That Suits You

Description

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