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All of the following are methods that can be used to isolate bacteria from a mixed culture EXCEPT?
All of the following are methods that can be used to isolate bacteria from a mixed culture EXCEPT?
Which organelle is involved in the packaging of proteins for secretion?
Which organelle is involved in the packaging of proteins for secretion?
The internationally recognized reference for bacterial classification and identification is called Bergey's Manual.
The internationally recognized reference for bacterial classification and identification is called Bergey's Manual.
True
Which carbohydrate is the primary or preferred starting compound for glycolysis and the fermentation pathway?
Which carbohydrate is the primary or preferred starting compound for glycolysis and the fermentation pathway?
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The term aerobic, anaerobic, and microaerophilic are used to categorize microorganisms based on their ______ requirements.
The term aerobic, anaerobic, and microaerophilic are used to categorize microorganisms based on their ______ requirements.
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All of the following body sites normally have large numbers of resident microbiota EXCEPT?
All of the following body sites normally have large numbers of resident microbiota EXCEPT?
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Which of the following items would be disposed of into the sharps container?
Which of the following items would be disposed of into the sharps container?
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Most human pathogens are classified as thermophiles.
Most human pathogens are classified as thermophiles.
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If an organism produces stable acids as an end-product of glucose fermentation, the methyl red test (MR test) will be red, indicating a positive result.
If an organism produces stable acids as an end-product of glucose fermentation, the methyl red test (MR test) will be red, indicating a positive result.
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Which statement correctly describes the level of contamination on different objects?
Which statement correctly describes the level of contamination on different objects?
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How is a glass culture tube handled during aseptic transfers?
How is a glass culture tube handled during aseptic transfers?
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When is it crucial to wear protective eyewear when working in the lab?
When is it crucial to wear protective eyewear when working in the lab?
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Which of the following sequences would result from the replication of the following DNA sequence: ATTGCTGGA?
Which of the following sequences would result from the replication of the following DNA sequence: ATTGCTGGA?
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Microorganisms can be described as being ubiquitous which means that they:
Microorganisms can be described as being ubiquitous which means that they:
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Which of the following diseases are caused by acid-fast bacterium:
Which of the following diseases are caused by acid-fast bacterium:
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Which of the following is a chemical means of controlling microbial growth?
Which of the following is a chemical means of controlling microbial growth?
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Catalase breaks hydrogen peroxide down in water and carbon dioxide.
Catalase breaks hydrogen peroxide down in water and carbon dioxide.
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How is a respirometer used to measure fermentation in the Cellular Respiration experiment?
How is a respirometer used to measure fermentation in the Cellular Respiration experiment?
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Gram negative cells appear purple after Gram staining whereas gram positive cells appear pink.
Gram negative cells appear purple after Gram staining whereas gram positive cells appear pink.
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The cloudy growth seen in a broth culture is referred to as:
The cloudy growth seen in a broth culture is referred to as:
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Which test uses a medium that is both selective and differential for members of the genus Staphylococcus?
Which test uses a medium that is both selective and differential for members of the genus Staphylococcus?
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In addition to linking together nucleotides to synthesize a daughter strand, DNA polymerase also ______.
In addition to linking together nucleotides to synthesize a daughter strand, DNA polymerase also ______.
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An antibiotic which interferes with the activity of ribosomes would have the most effect on which of the following?
An antibiotic which interferes with the activity of ribosomes would have the most effect on which of the following?
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Human skin provides an environment with NaCl levels slightly above that of most bacterial cells. Which organism would most likely be found on the skin?
Human skin provides an environment with NaCl levels slightly above that of most bacterial cells. Which organism would most likely be found on the skin?
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All of the following are found in most prokaryotic cells EXCEPT:
All of the following are found in most prokaryotic cells EXCEPT:
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The smallest living units of the human body are __________.
The smallest living units of the human body are __________.
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An organism has been shown to produce hydrogen sulfide and indole in its metabolism. These are examples of _____________ characteristics that can be helpful in the identification of the microorganism.
An organism has been shown to produce hydrogen sulfide and indole in its metabolism. These are examples of _____________ characteristics that can be helpful in the identification of the microorganism.
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All of the following are common to all cells at some point in their development EXCEPT:
All of the following are common to all cells at some point in their development EXCEPT:
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The purpose of heat fixation is to _______.
The purpose of heat fixation is to _______.
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Which of the following is true of a bacterial species that produces dark colonies when grown on MacConkey agar?
Which of the following is true of a bacterial species that produces dark colonies when grown on MacConkey agar?
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When first viewing a specimen using the brightfield microscope, it is recommended that you begin viewing the specimen with which objective lens?
When first viewing a specimen using the brightfield microscope, it is recommended that you begin viewing the specimen with which objective lens?
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In living organisms, information for making proteins flows from _______.
In living organisms, information for making proteins flows from _______.
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An antimicrobial, such as isoniazid, that is only effective against mycobacterial species would be referred to as a __________ drug.
An antimicrobial, such as isoniazid, that is only effective against mycobacterial species would be referred to as a __________ drug.
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The ability to distinguish fine detail or discriminate between two very closely spaced objects is called:
The ability to distinguish fine detail or discriminate between two very closely spaced objects is called:
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Which type of reaction(s) requires oxygen as a substrate?
Which type of reaction(s) requires oxygen as a substrate?
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Phenol red carbohydrate broths with Durham tubes can detect all of the following EXCEPT:
Phenol red carbohydrate broths with Durham tubes can detect all of the following EXCEPT:
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The monomers that make up DNA molecules are called _______.
The monomers that make up DNA molecules are called _______.
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The process of forming mRNA from the template strand of DNA is called _____________.
The process of forming mRNA from the template strand of DNA is called _____________.
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Immersion oil must only be used with which objective lens?
Immersion oil must only be used with which objective lens?
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The sources of all antimicrobial drugs are fungi and other microorganisms.
The sources of all antimicrobial drugs are fungi and other microorganisms.
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In the Gram stain, the chemical that fixes dye in or on cells by forming an insoluble compound is referred to as the _______________.
In the Gram stain, the chemical that fixes dye in or on cells by forming an insoluble compound is referred to as the _______________.
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A pure culture contains:
A pure culture contains:
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All of the following are true of an enzyme-catalyzed reaction EXCEPT?
All of the following are true of an enzyme-catalyzed reaction EXCEPT?
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Before attempting to identify an unknown bacterial species, the organism must be isolated in a pure culture.
Before attempting to identify an unknown bacterial species, the organism must be isolated in a pure culture.
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When a large zone of no growth is found around an antibiotic disc being used for identification purposes, this generally means that the microbe is ______ to the antibiotic.
When a large zone of no growth is found around an antibiotic disc being used for identification purposes, this generally means that the microbe is ______ to the antibiotic.
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Stains are commonly used for all of the following EXCEPT:
Stains are commonly used for all of the following EXCEPT:
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All of the following organisms are expected to grow within an anaerobe jar EXCEPT:
All of the following organisms are expected to grow within an anaerobe jar EXCEPT:
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Eukaryotes have membrane-bound organelles.
Eukaryotes have membrane-bound organelles.
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A hypertonic solution is prepared by the addition of sucrose. Which of the following would happen to bacteria placed in this solution?
A hypertonic solution is prepared by the addition of sucrose. Which of the following would happen to bacteria placed in this solution?
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Which of the following processes removes or destroys endospores?
Which of the following processes removes or destroys endospores?
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Study Notes
Bacterial Isolation and Identification
- Various methods to isolate bacteria include streak plating, loop dilution, and serial dilution followed by spread plating; Gram staining is not an isolation method.
- Bergey's Manual is the authoritative reference for bacterial classification and identification.
Cellular Components and Functionality
- The Golgi apparatus is responsible for packaging proteins for secretion.
- Cell nucleus is absent in prokaryotic cells, while cell membrane, cytoplasm, and cell wall are common.
Microbial Classification
- Microorganisms are categorized as aerobic, anaerobic, or microaerophilic based on oxygen requirements.
- Most human pathogens are mesophiles, not thermophiles.
Laboratory Practices
- Used microscope slides should be disposed of in sharps containers.
- Protective eyewear is essential when handling hazardous chemicals in the lab.
Genetics and Metabolism
- The primary carbohydrate for glycolysis and fermentation is glucose.
- DNA polymerase "proofreads" nucleotides when synthesizing the daughter strand.
- Fermentation assessment can utilize a respirometer to measure gas bubble size produced.
Microbial Growth and Behavior
- Microorganisms impacted by sodium chloride (NaCl) on human skin include halotolerant organisms.
- Lactose fermenters produce dark colonies on MacConkey agar, contrasting with other species.
Microscopy
- The initial viewing of specimens under a brightfield microscope should start with a 4X scanning objective.
Antimicrobial Agents
- Antibiotics targeting ribosomes affect protein synthesis. An antibiotic specific to mycobacterial species is considered a narrow-spectrum drug.
- Sterilization is the process that effectively removes or destroys endospores.
Staining and Identification Techniques
- Heat fixation in microscopy adheres microorganisms to slides and prevents contamination during staining.
- Stains assist in disease diagnosis, pathogen identification, and organism classification, but not disease treatment.
Microbial Characteristics
- Ubiquitous microorganisms are found on nearly every surface.
- Physiological characteristics such as hydrogen sulfide and indole production assist in microorganism identification.
Other Key Concepts
- The process of forming mRNA from DNA is known as transcription.
- Resolution refers to distinguishing fine details between closely spaced objects in microscopy.
- A hypertonic sucrose solution leads to plasmolysis in bacteria due to osmotic pressure.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
Test your knowledge with this set of flashcards focusing on key concepts from microbiology lab work. The quiz covers methods for isolating bacteria and organelles involved in protein packaging, among other topics. Perfect for students looking to reinforce their understanding of microbiology.
