Microbiology Lab Manual: The Microscope and Its Use

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What type of microscope is particularly useful for examining unstained living cells and detailed internal structures?

Phase-contrast microscope

What is the purpose of the filter on the ocular lens in a fluorescence microscope?

To allow longer ultraviolet wavelengths to go through and block or eradicate shorter wavelengths

What type of microscopes use a beam of electrons to illuminate the specimen?

Transmission Electron Microscope (TEM)

What is the name of the technique that uses fluorescent antibodies as a diagnostic tool?

Fluorescent antibody technique (FAT)

What is the advantage of using confocal microscopy over other types of microscopy?

Generating high-resolution images and 3-D reconstructions of a specimen

What is the purpose of fluorochromes in fluorescence microscopy?

To absorb UV light and emit light within the visible spectrum

What is the difference between the light background in a phase-contrast microscope and the image of the specimen?

The image appears dark against a light background

What is the advantage of using computer-assisted scanning in confocal microscopy?

Simultaneous imaging of multiple fluorochromes

What is the purpose of thin sectioning or freeze fracturing in Transmission Electron Microscope (TEM) preparation?

To carefully prepare the specimen for examination

What type of microscopy is used for immunofluorescence and diagnostic applications?

Fluorescence microscope

Learn about the microscope and its importance in microbiology. Discover how Antoni van Leeuwenhoek's invention revolutionized the field and increased our understanding of the living world.

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