Microbiology Lab Manual: The Microscope and Its Use
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Questions and Answers

What type of microscope is particularly useful for examining unstained living cells and detailed internal structures?

Phase-contrast microscope

What is the purpose of the filter on the ocular lens in a fluorescence microscope?

To allow longer ultraviolet wavelengths to go through and block or eradicate shorter wavelengths

What type of microscopes use a beam of electrons to illuminate the specimen?

Transmission Electron Microscope (TEM)

What is the name of the technique that uses fluorescent antibodies as a diagnostic tool?

<p>Fluorescent antibody technique (FAT)</p> Signup and view all the answers

What is the advantage of using confocal microscopy over other types of microscopy?

<p>Generating high-resolution images and 3-D reconstructions of a specimen</p> Signup and view all the answers

What is the purpose of fluorochromes in fluorescence microscopy?

<p>To absorb UV light and emit light within the visible spectrum</p> Signup and view all the answers

What is the difference between the light background in a phase-contrast microscope and the image of the specimen?

<p>The image appears dark against a light background</p> Signup and view all the answers

What is the advantage of using computer-assisted scanning in confocal microscopy?

<p>Simultaneous imaging of multiple fluorochromes</p> Signup and view all the answers

What is the purpose of thin sectioning or freeze fracturing in Transmission Electron Microscope (TEM) preparation?

<p>To carefully prepare the specimen for examination</p> Signup and view all the answers

What type of microscopy is used for immunofluorescence and diagnostic applications?

<p>Fluorescence microscope</p> Signup and view all the answers

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