Microbiology in Food Production

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Questions and Answers

Which type of bacteria is primarily associated with the fermentation of rye bread?

  • Saccharomyces cerevisiae
  • Lactobacillus plantarum (correct)
  • E. coli
  • Bacillus subtilis

What is the main product of yeast fermentation in bread dough?

  • Oxygen
  • Acetic acid
  • Ethanol
  • Carbon dioxide (correct)

What role does kneading play in bread-making?

  • Adds sugar to the dough
  • Increases alcohol content
  • Incorporates air into the dough (correct)
  • Decreases gluten structure

Which of the following is NOT a source of fermentable sugar for yeast metabolism?

<p>Protein (D)</p> Signup and view all the answers

What is Single-cell protein (SCP) primarily used for?

<p>As a source of crude or refined protein for animal feed and human food (D)</p> Signup and view all the answers

What raw materials can be used in the production of Single-cell protein?

<p>Waste materials such as molasses, agricultural, and kitchen wastes (D)</p> Signup and view all the answers

What is one key aspect that differentiates home-baked bread from commercial bread?

<p>Rate of fermentation (A)</p> Signup and view all the answers

Which process results in the production of a transgenic organism?

<p>Genetic engineering (D)</p> Signup and view all the answers

Which microorganism is responsible for producing the animal feed known as Pruteen?

<p>Methylophilus methylotrophus (C)</p> Signup and view all the answers

What type of acid is produced along with carbon dioxide during bread fermentation?

<p>Lactic acid (C)</p> Signup and view all the answers

How is Vitamin B12 currently produced?

<p>Fermentation using propionic bacteria (B)</p> Signup and view all the answers

Which of the following yeast strains is most commonly utilized in baking?

<p>Baker's yeast (B)</p> Signup and view all the answers

What microbe is Spirulina classified as?

<p>A spiral-shaped cyanobacterium (blue-green algae) (D)</p> Signup and view all the answers

What process is used to produce cultured buttermilk?

<p>Adding Streptococcus cremoris to pasteurized skim milk (A)</p> Signup and view all the answers

Which vitamin was the first to be produced through fermentation?

<p>Vitamin C (D)</p> Signup and view all the answers

What characteristic of mycoprotein makes it suitable for meat substitutes?

<p>It has a filamentous texture (D)</p> Signup and view all the answers

What is the primary function of glyphosate as described?

<p>To block an enzyme essential for plant growth (C)</p> Signup and view all the answers

How are plant cells made resistant to glyphosate?

<p>By introducing a gene for glyphosate breakdown (C)</p> Signup and view all the answers

What is one potential negative outcome of cultivating insect-resistant crops?

<p>Evolution of resistance by the insect pests (A)</p> Signup and view all the answers

What role does Agrobacterium tumefaciens play in genetic modification?

<p>It infects plant cells to transfer DNA (D)</p> Signup and view all the answers

Which of the following crops has been modified to resist the corn borer?

<p>Maize (D)</p> Signup and view all the answers

What is a common method for selecting plant cells that have taken up the plasmid containing the glyphosate resistance gene?

<p>Growing them on a medium with an antibiotic (B)</p> Signup and view all the answers

Which of the following is an example of a genetically modified crop mentioned?

<p>BT-Cotton (B)</p> Signup and view all the answers

Which of the following describes a consequence of the genetic modification of crops for pest resistance?

<p>Potential damage to other insect species (B)</p> Signup and view all the answers

What is the primary function of electrodes in bioelectronics?

<p>To carry electrical signals from living organisms to storage devices (B)</p> Signup and view all the answers

Which substance is NOT mentioned as being measurable by biosensors?

<p>Carbon dioxide (D)</p> Signup and view all the answers

What is one application of the handheld aflatoxin detection system?

<p>Monitoring food quality for aflatoxins (C)</p> Signup and view all the answers

How do forensic biologists contribute to paternity cases?

<p>Through DNA analysis of specimens to establish relationships (D)</p> Signup and view all the answers

Which of the following is NOT a characteristic of biosensors?

<p>They only measure pollutants in water sources. (C)</p> Signup and view all the answers

In forensic science, what type of evidence is analyzed for DNA?

<p>Blood, saliva, and hair samples (D)</p> Signup and view all the answers

What recent advancement has been made for biosensor durability and portability?

<p>Handheld systems that detect aflatoxins (D)</p> Signup and view all the answers

What key role do fingerprints play in forensic science?

<p>They serve as unique identifiers for individuals. (C)</p> Signup and view all the answers

Which statement accurately describes the function of antibiotics produced by microorganisms?

<p>They are complex organic compounds synthesized by the microorganisms. (C)</p> Signup and view all the answers

What differentiates bacteriostatic antibiotics from bacteriocidal antibiotics?

<p>Bacteriostatic antibiotics prevent bacteria from reproducing, while bacteriocidal antibiotics kill the bacteria. (C)</p> Signup and view all the answers

Why do antibiotics typically not damage human cells?

<p>Animal cells lack cell walls and have different protein production structures. (A)</p> Signup and view all the answers

Which microorganism is primarily responsible for producing streptomycin?

<p>Streptomyces (C)</p> Signup and view all the answers

What was significant about the discovery of penicillin by Sir Alexander Fleming?

<p>It introduced the concept of using a naturally occurring substance as an antibiotic. (A)</p> Signup and view all the answers

What has changed about the production of penicillin since its discovery?

<p>Different mutant forms of Penicillium are now used to produce it. (D)</p> Signup and view all the answers

Which of the following antibiotics is NOT a chemically altered form of penicillin?

<p>Streptomycin (A)</p> Signup and view all the answers

What is the primary source of most antibiotics used today?

<p>Fungi and bacteria found in the soil (C)</p> Signup and view all the answers

What is the primary benefit of inserting Bacillus thuringiensis (Bt) genes into cotton crops?

<p>To develop proteins that are toxic to insects (B)</p> Signup and view all the answers

Which of the following best describes transgenic animals?

<p>Animals that express recombinant DNA to produce human proteins (A)</p> Signup and view all the answers

What are transgenic plants primarily developed to achieve?

<p>Creating plants with desirable traits such as disease resistance (D)</p> Signup and view all the answers

Why are transgenic plants closely monitored by government agencies?

<p>To confirm they are safe for human consumption and the environment (C)</p> Signup and view all the answers

What is cloning in the context of biotechnology?

<p>Producing identical copies of genes, cells, or organisms (D)</p> Signup and view all the answers

Which recombinant proteins are commonly produced in the milk of transgenic goats?

<p>Human α-antitrypsin and Human protein C (D)</p> Signup and view all the answers

What is a significant risk associated with the use of transgenic plants?

<p>They may share foreign genes with other species in the environment (A)</p> Signup and view all the answers

What is one of the main advantages of cloning in biotechnology?

<p>To produce identical copies with desirable characteristics (B)</p> Signup and view all the answers

Flashcards

Microbial Food Production

A method of increasing food production using microorganisms.

Single-Cell Protein (SCP)

A protein source derived from single-celled organisms, like bacteria, algae, or fungi.

Mycoprotein

A type of SCP made from the fungus Fusarium venenatum, known for having a texture resembling meat.

Spirulina

A spiral-shaped cyanobacterium, often sold as a health food, that serves as a nutrient source in some regions.

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Microbial Vitamin Production

Vitamins, like vitamin C and B12, are produced using fermentation processes that involve bacteria.

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Cultured Buttermilk

A popular dairy product made by adding Streptococcus cremoris to pasteurized skim milk, resulting in fermented sourness.

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Sour Cream

Cream that is fermented by adding certain bacteria, like Streptococcus lactis, to achieve a tangy flavor.

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Yogurt Production

The process of using microorganisms to convert milk into yogurt, with the help of bacteria like Lactobacillus bulgaricus and Streptococcus thermophilus.

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Yeast in Bread Making

Yeast, a type of fungus, plays a crucial role in bread making. It ferments sugar, producing carbon dioxide that causes dough to rise. This process also generates subtle flavors in the bread.

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Yeast Respiration in Bread

The fermentation of sugar by yeast in bread dough creates carbon dioxide and water. This process is aerobic, meaning it happens in the presence of oxygen.

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Lactic Acid Bacteria in Bread

Lactic acid bacteria, like Lactobacillus plantarum and Leuconostoc mesenteroides, play a significant role in developing specific flavors in bread. They contribute to the characteristic tang of sourdough bread and add a unique taste to rye bread.

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Sourdough Starter

A traditional sourdough starter is a mixture of flour and water left to ferment naturally. This fermentation occurs due to the presence of wild yeasts and bacteria in the environment, like those found in the air, on the grain, or even on the baker's hands.

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Genetic Engineering: The Basics

Genetic engineering involves making changes to an organism's DNA by introducing DNA from another organism. It's used to create transgenic organisms with altered traits, such as crops resistant to pests or animals producing specific pharmaceuticals.

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Animal Breeding

Animal breeding focuses on improving the genetic characteristics of livestock. By carefully selecting and breeding animals with desirable traits, breeders can enhance productivity and other desired qualities in future generations.

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Transgenic Organisms

Transgenic animals and plants are created using genetic engineering techniques. These organisms have had foreign DNA inserted into their genomes, resulting in modifications to their traits, such as disease resistance or improved crop yield.

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Genetic Engineering and Pharmaceuticals

Genetic engineering plays a role in the production of pharmaceuticals by introducing desired genes into organisms like bacteria or yeast. These organisms then produce the target drugs or proteins that can be extracted and used for medical treatments.

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Biosensor

A device that converts biological reactions into measurable electrical signals by linking living organisms, their enzymes or organelles, with electrodes.

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Electrode

A solid conductor carrying electric current into non-metallic substances like liquids, gases, or plasmas.

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Bioelectronics

The study of using biological principles to develop and improve electronic devices.

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Forensic Biology

The analysis of biological evidence, like blood, saliva, or hair, to identify individuals or determine cause of death.

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Fingerprints

Unique patterns of ridges and valleys on fingertips used for identification.

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Paternity Case

The process of determining a child's biological father using DNA analysis.

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Biosensor Applications

A method of detecting specific substances, such as pollutants or food components, using biological reactions.

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Immunochemical-Based Biosensors

A type of biosensor that utilizes immunochemical reactions to detect specific substances.

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Antibiotics

Substances produced by certain microorganisms, usually bacteria or fungi, that can inhibit the growth or kill other microorganisms, particularly bacteria.

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Actinomycetes

Filamentous bacteria resembling microscopic fungi, known for their ability to produce various antibiotics.

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Bacteriostatic

Antibiotics that inhibit bacterial growth without killing them directly, preventing bacterial multiplication.

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Bactericidal

Antibiotics that kill bacteria directly.

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Antibiotic Manufacture

The process of creating antibiotics by utilizing microorganisms, typically in controlled environments. This involves cultivating bacteria or fungi and extracting the antibiotics they produce.

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Penicillin

A type of antibiotic produced by the Penicillium fungus, known for its effectiveness against a range of bacterial infections.

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Fermenter

A large vessel used for industrial-scale production of antibiotics, often penicillin, where the microorganisms are grown and the antibiotic is extracted.

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What is a tumor?

An abnormal mass of tissue that forms when cells grow and divide more than they should or do not die when they should.

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What is Agrobacterium tumefaciens?

A soil bacterium that can infect plant cells and transfer a specific sequence of its DNA into the plant cell, causing crown gall disease.

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What is glyphosate?

A widely used herbicide that kills weeds and grasses by blocking an enzyme essential for plant growth.

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What is the glyphosate resistance gene?

A gene that makes plants resistant to glyphosate herbicide.

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How can plants be made resistant to glyphosate?

A genetic modification process that introduces a gene for an enzyme that breaks down glyphosate into plants, reducing the need for herbicide.

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What are pest resistant GM crops?

Genetically modified (GM) crops designed to be toxic to certain insects, primarily cotton and maize.

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What is a potential problem with pest resistant GM crops?

The evolution of resistance by insect pests to GM crops, leading to their effectiveness decreasing over time.

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What is a potential environmental concern with GM crops?

The potential for genes from GM crops to transfer to other species of plants through cross-pollination, potentially introducing new traits into the environment.

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BT-Cotton

A type of genetically modified cotton that produces a protein toxic to insects, reducing pest damage and increasing yield.

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Transgenic Animals

Animals that have been genetically modified to express recombinant DNA, often producing human proteins in their milk or eggs.

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Transgenic Plants

Plants that have received and express DNA from another species, often engineered for traits like disease resistance, herbicide tolerance, or improved nutritional value.

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Cloning

The process of creating a genetically identical copy of a gene, cell, or organism. Often used in agriculture to produce multiple copies of plants with desirable traits.

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Delta-Endotoxin

A protein (delta-endotoxin) produced by Bacillus thuringiensis bacteria, highly toxic to specific insects.

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Disease Resistance in Transgenic Plants

Genes from a different species are introduced into a plant's genome, making it resistant to specific diseases.

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Herbicide Resistance in Transgenic Plants

Genes from a different species are introduced into a plant's genome, making it resistant to specific herbicides.

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Improved Nutritional Value in Transgenic Plants

Genes from a different species are introduced into a plant's genome, improving its nutritional content, often increasing vitamins or essential nutrients.

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Study Notes

Biology Grade 12 Unit 1: Application of Biology

  • Biology is the science that studies all living things, from bacteria to whales.
  • It encompasses understanding organisms from the smallest (0.1 micrometer bacteria) to the largest (90,000 to 136,000 kilograms blue whales).

Applications of Biology

  • Biologists study specific subsets of living organisms (e.g., birds, plants, bacteria).
  • They investigate disease origins and effects on plants and animals.
  • They examine the functions of living organisms (physiology, biochemistry).
  • Evolutionary biologists study the evolution of species.
  • Pharmaceutical scientists discover new drugs.
  • Genetic engineers utilize genetic engineering.
  • Cytologists research applications using mother cells.
  • Zoologists study animals and their behaviors.
  • Botanists study plants.
  • Microbiologists, Mycologists, and Pathologists examine microorganisms.
  • Environmental scientists study global warming.

Application in Conservation of Natural Resources

  • Natural resources, including soil, water, air, plants, animals, and energy, exist independent of human action.
  • Renewable resources can be produced endlessly (e.g., sunlight, crops, water, trees, livestock, wind).
  • Non-renewable resources exist in a fixed amount and cannot easily be replaced (e.g., fossil fuels, minerals, nuclear fuels).
  • Conservation involves preserving natural resources.
  • Ethiopia is rich in natural resources, which include various minerals (e.g., gold, platinum, potash, limestone) and natural resources, as well as lakes & water falls.
  • Timber, many crop plants, coffee plantations, diverse animals, and birds form rich ecosystems, representing renewable resources.
  • Examples of renewable resources in Ethiopia include coffee, Walia Ibex, sunlight, and the Ethiopian Bush crow.
  • Main sources of renewable resources include agricultural products, solar energy, wind energy, hydropower, geothermal energy, tidal energy, wave energy, and biofuels.

Non-Renewable Resources

  • Non-renewable resources are natural substances that are not replaced at the same rate as they are used.
  • Examples of non-renewable resources include metals (e.g., gold, iron) fossil fuels (e.g., oil, natural gas, coal), and others.
  • Conservation of non-renewable resources is important given their negative environmental impact (e.g., climate change).

Energy Sources

  • Energy sources are classified into renewable and non-renewable types.
  • Renewable energy sources include wind, hydropower, solar, geothermal energy, biomass, etc.
  • Non-renewable energy sources include oil, coal, nuclear fuels, etc.

Environmental Impacts of Mining

  • Mining activities can harm natural habitats.
  • Oil, fuel, and chemical spills can contaminate surrounding environments (soil, rivers, groundwater).
  • Mining operations can cause air pollution.

Conservation Biology

  • Conservation biology aims to protect and restore biodiversity.
  • This field focuses on preserving the diversity of life on Earth.

Food and Nutrition Security

  • Food security for all people includes achieving physical, social, and economic access to sufficient, safe, and nutritious food to meet diverse needs, at all times.
  • Availability of food, Accessibility to food, and Affordability of food are all important aspects of food security.
  • Nutrition security involves individuals having access to sufficient, safe, and nutritious food, safe water, and adequate sanitation.
  • Health care services, household, and community practices for preparing and storing food, and hygiene practices.
  • Prolonged lack of nutrition from poverty can cause stunted growth, slow cognitive development, and increased susceptibility to illness.

Biotechnology Applications

  • Biotechnology involves applying technologies using living organisms, or their products, for producing goods beneficial to humans.
  • Genetically modified organisms (GMOs) incorporate genetic material via recombinant DNA technology.
  • Transgenic organisms receive genetic material from other species.
  • A gene from one species introduced into another is called a transgene.
  • Biotechnology is used in various products like pharmaceuticals, gene therapy, and transgenic plants/animals.

Biomining

  • Biomining employs microbes to extract minerals, as ore concentrations decrease.
  • A key example is the bacterium Thiobacillus ferrooxidans, which oxidizes sulfur, enabling the release of metals like copper, zinc, lead, and uranium.

Solid Waste Treatment

  • Solid waste, especially non-biodegradable components (metals, plastics), is often treated in landfills.
  • Biodegradable components (food waste, textiles) decompose over time, often at varying speeds.
  • Modern landfills may have systems to manage methane to prevent hazards. - Composting accelerates the decomposition of organic materials, creating humus.

Wastewater Treatment

  • Wastewater treatment aims to eliminate undesirable substances and microorganisms before water enters natural environments. - Treatment commonly involves physical processes to remove floating debris and sedimentation to reduce biochemical oxygen demand.
  • Secondary treatment involves microbial decomposition of organic matter in aerobic environments like trickling filters or activated sludge basins, reducing BOD.
  • Sludge produced can be further processed through anaerobic digestion for biogas generation, water treatment, or soil conditioners.

Bioremediation

  • Bioremediation utilizes living organisms to detoxify and remove environmental pollutants.
  • Saprotrophic microbes break down a wide range of substances, including toxic ones (like mercury) with a process called biodegradation.
  • Bioremediation can convert harmful substances into less harmful substances, using biotransformation.

Biofuels

  • Biofuels are alternative to fossil fuels, often produced by fermenting plant materials (like corn) or crop residues (e.g. cellulose/hemicellulose).
  • Biogas production involves anaerobic breakdown of organic waste matter (manure, crops, etc.) by bacteria/archeans to produce methane, valuable as an energy source.
  • Biofuels/biogas may be used for cooking, lighting, and alternative energy sources.

Composting

  • Composting utilizes aerobic microorganisms to convert solid organic matter into stable humus, effectively reducing waste bulk, and improving soil conditions though the creation of nutrients.

Cloning

  • Cloning is a method of producing identical copies of biological materials (genes, cells, or organisms).
  • Clones are genetically identical organisms or cells resulting from asexual reproduction or man-made cloning techniques.

Biological Warfare

  • Biological warfare involves using pathogens to harm people, animals, or the environment.
  • Biological weapons can range from a simple hoax to deadly agents capable of producing widespread devastation.

Applications in Industry

  • Enzymes—produced by commercially fermenting readily available feedstocks (corn steep liquor, molasses)—are used in various industries, and often produced by non-pathogenic organisms.

Additional Topics

  • Fermented milk—some are made using organisms like Lactobacillus—and fermented meats are common practices in various societies.
  • Beer and wine are made by fermenting sugary products using yeast.

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