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A media usually composed of meat and soybean extracts. It support growth of most non-fastidious bacteria to grow at natural rates.
A media usually composed of meat and soybean extracts. It support growth of most non-fastidious bacteria to grow at natural rates.
Blood Agar Plate
PH indicator used for MacConkey agar.
PH indicator used for MacConkey agar.
Neutral Red
Enrichment video used to isolate Neisseria Gonorrhoeae and Haemophilus influenzae.
Enrichment video used to isolate Neisseria Gonorrhoeae and Haemophilus influenzae.
Chocolate Agar Plate
Enumerate at least two inhibitory agents for gram negative bacteria.
Enumerate at least two inhibitory agents for gram negative bacteria.
Inoculate by shaking a previously heated wire loop or needle. It uses also of Pasteur pipette.
Inoculate by shaking a previously heated wire loop or needle. It uses also of Pasteur pipette.
Usually measured in millimeters or described as pinpoint, small, medium, large.
Usually measured in millimeters or described as pinpoint, small, medium, large.
Presumptive identification of streptococci and enterococci.
Presumptive identification of streptococci and enterococci.
Gram positive bacteria produces larger colonies than a gram negative bacteria?
Gram positive bacteria produces larger colonies than a gram negative bacteria?
Raised or bulging center, convex and outie.
Raised or bulging center, convex and outie.
What is the color of most Enterobacteriaceae on Blood Agar Plate.
What is the color of most Enterobacteriaceae on Blood Agar Plate.
In determining the consistency of the colonies of bacteria what instrument is appropriate to used?
In determining the consistency of the colonies of bacteria what instrument is appropriate to used?
Formerly known as Enterococus.
Formerly known as Enterococus.
Bacteria with filamentous appearance colonies.
Bacteria with filamentous appearance colonies.
Culture media used to observe hemolytic pattern of Streptococcus.
Culture media used to observe hemolytic pattern of Streptococcus.
Granulated and rough colonies is hard to emulsify with Normal Saline (TRUE/FALSE).
Granulated and rough colonies is hard to emulsify with Normal Saline (TRUE/FALSE).
Produced by mutant strains that lack of surface proteins indicating loss of virulence.
Produced by mutant strains that lack of surface proteins indicating loss of virulence.
Characteristic colony that are produced by Klebsiella Pneumoniae.
Characteristic colony that are produced by Klebsiella Pneumoniae.
Odors can be helpful as secondary identification of bacteria? (TRUE/FALSE)
Odors can be helpful as secondary identification of bacteria? (TRUE/FALSE)
How many percent of agar is present to isolate a solid medium.
How many percent of agar is present to isolate a solid medium.
Tissue culture medium used to isolate and grow Chlamydia Trachomatis|
Tissue culture medium used to isolate and grow Chlamydia Trachomatis|
It used for identifying surface colonies in developing the growth of microorganisms.
It used for identifying surface colonies in developing the growth of microorganisms.
What is the adjusted pH used in Alkaline Peptone water.
What is the adjusted pH used in Alkaline Peptone water.
How many percent of agar is present in Thioglycollate Broth.
How many percent of agar is present in Thioglycollate Broth.
Selective enrichment broth for isolation of Salmonella and Proteus.
Selective enrichment broth for isolation of Salmonella and Proteus.
Used to increase the number of microorganisms with unusual physiologic characteristics.
Used to increase the number of microorganisms with unusual physiologic characteristics.
Flashcards
Single Molt Media
Single Molt Media
A type of growth media that supports the growth of a wide variety of bacteria, typically composed of meat and soybean extracts.
pH indicator
pH indicator
A chemical that changes color depending on the acidity or alkalinity of the solution.
Enrichment Media
Enrichment Media
A type of media used to increase the number of specific microorganisms in a sample, making them easier to isolate.
Inhibitory Agent
Inhibitory Agent
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Inoculation
Inoculation
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Colony Size
Colony Size
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Presumptive Identification
Presumptive Identification
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Colony Morphology
Colony Morphology
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Blood Agar Plate (BAP)
Blood Agar Plate (BAP)
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Hemolytic Patterns
Hemolytic Patterns
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Granulated & Rough Colonies
Granulated & Rough Colonies
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Bacteria with Filamentous Appearance
Bacteria with Filamentous Appearance
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Characteristic Colony of Klebsiella Pneumoniae
Characteristic Colony of Klebsiella Pneumoniae
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Agar Percentage in Solid Medium
Agar Percentage in Solid Medium
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Tissue Culture Medium
Tissue Culture Medium
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Selective Enrichment Broth
Selective Enrichment Broth
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Study Notes
Part II: Identification
- Media Composition: A media typically composed of meat, soybean extracts, supporting growth for most non-fastidious bacteria at natural rates
- pH Indicator: Neutral red is a pH indicator used for Mac Conkey agar
- Enrichment: Techniques to isolate Neisseria Gonnorheae and Haemophilus Influenzae utilizing a video.
- Inhibitory Agents: Telurite and sodium tellurite are inhibitory agents for gram-negative bacteria
- Specimen Inoculation: Inoculation performed using wire loops or needles, previously heated and Pasteur pipette.
- Colony Size Measurement: Colony sizes are typically described in millimeters, including pinpoint, small, medium or large sizes.
- Streptococci and Enterococci Identification: Used for presumptive identification
- Gram-Positive vs. Gram-Negative Colonies Differentiation: Gram-positive bacteria tend to produce larger colonies than gram-negative bacteria.Â
- Colony Morphology: Raised or bulging center, convex outline are characteristics of some colony types, helpful for classification.
- Enterobacteriaceae Color: Most Enterobacteriaceae colonies appear on Blood Agar Plates as gray or opaque
- Bacteria with Filamentous Appearance: Bacteria with filamentous colonies, such as P. anthracis
- Hemolytic Patterns: Use of Blood Agar plates (BAP) to observe hemolytic patterns of certain streptococci
- Emulsifiability with Normal Saline: Granulated and rough colonies are difficult to emulsify in normal saline
- Mutant Strains and Virulence: Some mutant strains lack surface proteins, thus reducing virulence
- Secondary Identification: Odors can be a useful feature for secondary identification of bacteria (e.g. Klebsiella pneumoniae)
Other Topics Notes
- Agar Percentage: 1.6 -20% agar is typically present in solid media
- Chlamydia Trachomatis Isolation: Tissue culture media is often used for isolating and cultivating Chlamydia Trachomatis
- pH Adjustment: Alkaline peptone water is used to adjust the pH for microbiological growth.Â
- Thioglycollate Broth: Usually contains 0.075% agar
- Selective Enrichment Broth: Selective enrichment broths (e.g., for Salmonella and Proteus) are used to increase the number of specific microorganisms while inhibiting growth of others
- Microbial Physiological Characteristics Identification: Used to increase the number of specific microorganisms while inhibiting growth of others
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