Questions and Answers
What is the first step in the endospore stain procedure?
Place the slide on a beaker with boiling water.
What is done in the second step of the endospore stain procedure?
Saturate slide with malachite green.
How long should the stain be applied during the third step?
Stain for 5-6 minutes.
What is the action performed in the fourth step of the endospore stain?
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What should be done to the slide in the fifth step?
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In the sixth step, what is added to the smear?
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What is done to the slide in the seventh step?
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What is the final step in the endospore stain procedure?
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Study Notes
Endospore Staining Steps
- First, place the prepared slide over a beaker of boiling water to provide heat.
- Saturate the slide with malachite green, which acts as the primary stain in the procedure.
- Allow the stain to process for 5-6 minutes, ensuring to add more malachite green if it evaporates during staining.
- After staining, use forceps to carefully remove the slide and let it cool down.
- Rinse the slide with water to wash away any excess malachite green staining.
- Apply safranin to the smear and leave it for 60-90 seconds; this acts as a counterstain.
- Rinse the slide again with water to eliminate excess safranin.
- Finally, blot the slide gently using bibulous paper to remove moisture without smearing the stain.
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Description
This quiz covers the step-by-step process of endospore staining, essential for identifying bacterial endospores. Students will learn the importance of heat fixation, proper staining techniques with malachite green and safranin, and the significance of each step in microbiological practices.
