Microbiology: Halophiles and Acidophiles
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Questions and Answers

Which of the following best describes organisms that require high osmotic pressure for growth?

  • Acidophiles
  • Psychrophiles
  • Thermophiles
  • Halophiles (correct)

What is the water activity (a_w) range for most halophilic bacteria?

  • 0.6 - 0.8
  • 0.4 - 0.6
  • 0.2 - 0.4
  • 0.0 - 0.2 (correct)

Which of the following is a key adaptation that allows halophiles to thrive in high-salt environments?

  • Forming spores to withstand harsh conditions
  • Accumulating organic solutes to balance the high external osmotic pressure (correct)
  • Requiring low pH (0-5.5) for optimal growth
  • Producing pigments to protect against UV radiation

What is the optimal growth temperature range for most halophilic bacteria?

<p>20-45°C (B)</p> Signup and view all the answers

Which of the following is a common habitat for halophilic microorganisms?

<p>High-salt environments (e.g., salt marshes, hypersaline lakes) (A)</p> Signup and view all the answers

What is the primary mechanism that allows halophiles to maintain their cellular water content in high-salt environments?

<p>Synthesizing organic compatible solutes (e.g., glycerol, betaine) (D)</p> Signup and view all the answers

What is the main reason that halophiles require high salt concentrations to maintain stability and activity?

<p>To maintain the integrity of the cell wall, proteins, and plasma membrane (A)</p> Signup and view all the answers

Which of the following is NOT a characteristic of halophiles?

<p>Produce acid and heat shock proteins to tolerate acidic environments (B)</p> Signup and view all the answers

What is the primary mechanism that allows halophiles to maintain an internal pH near neutrality despite the high external salt concentrations?

<p>They exchange potassium ions for protons across the plasma membrane (D)</p> Signup and view all the answers

Which of the following is a key adaptation that allows thermophilic microorganisms to grow at high temperatures?

<p>Stabilization of their proteins and DNA through hydrogen bonding and proline incorporation (D)</p> Signup and view all the answers

Which of the following is NOT a key characteristic of obligate anaerobic microorganisms?

<p>They are able to grow in the presence of oxygen (A)</p> Signup and view all the answers

What is the water activity (aw) range required for microbial growth?

<p>0.9 - 1.0 (D)</p> Signup and view all the answers

Which type of organisms grow over a wide range of water activity levels?

<p>Osmotolerant organisms (C)</p> Signup and view all the answers

What is the water activity range at which fungi can grow?

<p>0.5 - 0.7 (C)</p> Signup and view all the answers

What type of organisms require high osmotic pressure for growth?

<p>Osmophiles (B)</p> Signup and view all the answers

Which category of organisms are adapted to saline environments?

<p>Halophiles (A)</p> Signup and view all the answers

What is the water activity range implicated in spoilage of dry foods such as bread?

<p>0.5 - 0.7 (D)</p> Signup and view all the answers

What do osmophiles use to increase their internal osmotic concentration?

<p>&quot;Compatible solutes&quot; (D)</p> Signup and view all the answers

Which type of organisms require a high percentage of NaCl for growth?

<p>&quot;Halobacterium spp.&quot; (A)</p> Signup and view all the answers

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