Podcast
Questions and Answers
What is the best method to prove the role of a virulence gene according to the molecular version of Koch's postulates?
What is the best method to prove the role of a virulence gene according to the molecular version of Koch's postulates?
- Complementation of the mutation on a plasmid
- Transposon mutagenesis
- In-frame deletions or point mutations (correct)
- Introduction of mutation onto the chromosome of the pathogen
Which step of Koch's postulates involves isolating bacteria from diseased but not healthy animals?
Which step of Koch's postulates involves isolating bacteria from diseased but not healthy animals?
- Step 4 - Bacteria isolated from diseased animal
- Step 1 - Bacteria isolated from diseased but not healthy animals (correct)
- Step 2 - Bacteria grown in culture
- Step 3 - Bacteria from culture cause disease
What method often leads to the identification of auxiliary secretion systems or transcription factors in forward genetics?
What method often leads to the identification of auxiliary secretion systems or transcription factors in forward genetics?
- Reverse genetics
- Transposon mutagenesis (correct)
- RNAseq
- Cloning in E.coli K12
What is the main purpose of introducing a mutation onto the chromosome of a pathogen in reverse genetics?
What is the main purpose of introducing a mutation onto the chromosome of a pathogen in reverse genetics?
Which process involves characterizing putative virulence genes?
Which process involves characterizing putative virulence genes?
What is the phenotype or property under investigation associated with, according to the molecular version of Koch's postulates?
What is the phenotype or property under investigation associated with, according to the molecular version of Koch's postulates?
What is the significance of positive colonies appearing brick red on an indicator plate?
What is the significance of positive colonies appearing brick red on an indicator plate?
How does a positive colony on an indicator plate differentiate from a negative one?
How does a positive colony on an indicator plate differentiate from a negative one?
In the context of colony morphology, what does 'rough and smooth' refer to in relation to E.coli?
In the context of colony morphology, what does 'rough and smooth' refer to in relation to E.coli?
What aspect of pathogenesis is reflected by the pigmentation of Staphylococcus aureus colonies?
What aspect of pathogenesis is reflected by the pigmentation of Staphylococcus aureus colonies?
Which tissue culture model involves the extraction and growth of macrophages/DCs from bone marrow?
Which tissue culture model involves the extraction and growth of macrophages/DCs from bone marrow?
What distinguishes between primary cell lines and immortalized cells in tissue culture models?
What distinguishes between primary cell lines and immortalized cells in tissue culture models?
What is the purpose of staining with Diff-Quick in the intracellular growth curve experiment?
What is the purpose of staining with Diff-Quick in the intracellular growth curve experiment?
What is the significance of removing coverslips or hypotonically lysing host cells in the intracellular growth curve experiment?
What is the significance of removing coverslips or hypotonically lysing host cells in the intracellular growth curve experiment?
How does the process of washing monolayer with antibiotics affect the experiment?
How does the process of washing monolayer with antibiotics affect the experiment?
What role does the addition of gentamicin play in the experiment?
What role does the addition of gentamicin play in the experiment?
Why is it important to determine CFUs (gentamicin-resistant bacteria) during the experiment?
Why is it important to determine CFUs (gentamicin-resistant bacteria) during the experiment?
In the context of the experiment, what is the purpose of visualizing intracellular growth curves?
In the context of the experiment, what is the purpose of visualizing intracellular growth curves?
