Microbiology: Gram-Negative Bacteria Differentiation Quiz

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What is the best method to prove the role of a virulence gene according to the molecular version of Koch's postulates?

Complementation of the mutation on a plasmid

Transposon mutagenesis

In-frame deletions or point mutations (correct)

Introduction of mutation onto the chromosome of the pathogen

Which step of Koch's postulates involves isolating bacteria from diseased but not healthy animals?

Step 4 - Bacteria isolated from diseased animal

Step 1 - Bacteria isolated from diseased but not healthy animals (correct)

Step 2 - Bacteria grown in culture

Step 3 - Bacteria from culture cause disease

What method often leads to the identification of auxiliary secretion systems or transcription factors in forward genetics?

Reverse genetics

Transposon mutagenesis (correct)

RNAseq

Cloning in E.coli K12

What is the main purpose of introducing a mutation onto the chromosome of a pathogen in reverse genetics?

To identify virulence genes Signup and view all the answers

Which process involves characterizing putative virulence genes?

DNA sequencing and bioinformatics Signup and view all the answers

What is the phenotype or property under investigation associated with, according to the molecular version of Koch's postulates?

Pathogenic members of a genus or pathogenic strains of a species Signup and view all the answers

What is the significance of positive colonies appearing brick red on an indicator plate?

Lactose fermentation Signup and view all the answers

How does a positive colony on an indicator plate differentiate from a negative one?

Lactose fermentation Signup and view all the answers

In the context of colony morphology, what does 'rough and smooth' refer to in relation to E.coli?

LPS characteristics Signup and view all the answers

What aspect of pathogenesis is reflected by the pigmentation of Staphylococcus aureus colonies?

Anti-oxidant activity Signup and view all the answers

Which tissue culture model involves the extraction and growth of macrophages/DCs from bone marrow?

Primary cell lines Signup and view all the answers

What distinguishes between primary cell lines and immortalized cells in tissue culture models?

Life span Signup and view all the answers

What is the purpose of staining with Diff-Quick in the intracellular growth curve experiment?

To visualize the morphology of the colonies Signup and view all the answers

What is the significance of removing coverslips or hypotonically lysing host cells in the intracellular growth curve experiment?

To facilitate colony counting Signup and view all the answers

How does the process of washing monolayer with antibiotics affect the experiment?

Reduces bacterial contamination Signup and view all the answers

What role does the addition of gentamicin play in the experiment?

To kill extracellular bacteria Signup and view all the answers

Why is it important to determine CFUs (gentamicin-resistant bacteria) during the experiment?

To measure bacterial colony-forming units (CFUs) Signup and view all the answers

In the context of the experiment, what is the purpose of visualizing intracellular growth curves?

To determine antibiotic efficacy against intracellular pathogens Signup and view all the answers

Microbiology: Gram-Negative Bacteria Differentiation Quiz

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