Microbiology Exam Review Quiz

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Questions and Answers

The domains of life are

  • Bacteria, plants and animals
  • Bacteria, animals and eukarya
  • Bacteria, archaea and eukarya (correct)
  • Bacteria, algae and eukarya

Pili are also called

  • Fimbriae (correct)
  • Microvilli
  • Cilia
  • Flagella

It is FALSE about flagella that

  • Are used for movement in the environment
  • It is only present in Gram positive bacteria (correct)
  • Are present in bacteria
  • Have a hook at the end of the filament that anchored it to the basal body

Are infectious agents that do not have a nucleic acid genome.

<p>Viroids (B)</p> Signup and view all the answers

Are prokaryotic organism with cell wall but without peptidoglycan that likes to grow in extreme conditions

<p>Archaea (A)</p> Signup and view all the answers

Antibodies are produced by ______.

<p>plasma cells (D)</p> Signup and view all the answers

When two antibiotics are given together to increase the therapeutic effect the phenomenon is referred to as:

<p>Synergism (C)</p> Signup and view all the answers

What part of the growth curve should I use to calculate the doubling time?

<p>Log phase (D)</p> Signup and view all the answers

How many types of Biosafety levels there are?

<p>4 (A)</p> Signup and view all the answers

Used his skills at lens grinding and the use of light and improved the resolving power of the microscope to 200 times.

<p>Antony van Leeuwenhoek (D)</p> Signup and view all the answers

All ______ microscopes use visible light to illuminate enlarged images of specimens.

<p>Light (C)</p> Signup and view all the answers

Which of the following is not one of Koch's postulates?

<p>The microbe cannot be isolated and grown in pure culture outside of the host. (C)</p> Signup and view all the answers

Is the process of using microorganisms to clean up toxic, hazardous, or unmanageable compounds by degrading them to harmless compounds.

<p>Bioremediation (D)</p> Signup and view all the answers

Complex communities of microorganisms on surface are called:

<p>Biofilms (B)</p> Signup and view all the answers

The term microbiota refers to which of the following?

<p>All microorganisms in a certain region of the human body (A)</p> Signup and view all the answers

Methods of reproduction include:

<p>Cell division, binary fission, mitosis, and meiosis. (A)</p> Signup and view all the answers

Bacteria that have flagellum at both ends are referred to as

<p>Anphitrichous (C)</p> Signup and view all the answers

Chitin is a component of the cell wall of

<p>Fungi (B)</p> Signup and view all the answers

Compare Prokaryotes and Eukaryotes

<p>Prokaryotes are single-celled organisms that lack a nucleus and other membrane-bound organelles. Eukaryotes are organisms that have a nucleus and other membrane-bound organelles.</p> Signup and view all the answers

Name and briefly describe the different types of microscopes

<p>The different types of microscopes include light microscopes, electron microscopes, and scanning probe microscopes. Light microscopes use visible light to illuminate a specimen, and they can magnify objects up to 1,000 times. Electron microscopes use electrons to illuminate a specimen, and they can magnify objects up to 1 million times. Scanning probe microscopes use a sharp tip to scan a surface, and they can be used to create images of individual atoms.</p> Signup and view all the answers

Compare Gram positive and Gram negative bacteria

<p>Gram-positive bacteria have a thick layer of peptidoglycan in their cell walls, which stains purple with a Gram stain. Gram-negative bacteria have a thin layer of peptidoglycan in their cell walls, which stains pink with a Gram stain.</p> Signup and view all the answers

Name 2 types of bacterial cell morphology and name a bacteria with that morphology

<p>Two types of bacterial cell morphology are cocci and bacilli. Cocci are spherical bacteria. Bacilli are rod-shaped bacteria. <em>Staphylococcus aureus</em> is an example of a cocci bacteria. <em>Escherichia coli</em> is an example of a bacilli bacteria.</p> Signup and view all the answers

Compare enveloped and non-enveloped viruses

<p>Enveloped viruses have a lipid bilayer membrane surrounding their capsid. Non-enveloped viruses do not have a lipid bilayer membrane, and their capsid is exposed.</p> Signup and view all the answers

Name an opportunistic fungal infection and describe in what patient groups it might cause disease?

<p>Candidiasis is an opportunistic fungal infection that can cause disease in patients with weakened immune systems, such as people with HIV/AIDS or who have undergone organ transplantation. It can cause infections in the skin, mouth, vagina, and esophagus.</p> Signup and view all the answers

Compare and contrast general and selective growth media

<p>General growth media is a type of media that is designed to support the growth of a wide variety of microorganisms. Selective growth media is a type of media that is designed to support the growth of only certain types of microorganisms. This is achieved by adding specific ingredients to the media that inhibit the growth of certain microorganisms.</p> Signup and view all the answers

It is FALSE about flagella that are used for movement in the environment.

<p>False (B)</p> Signup and view all the answers

______ used his skills at lens grinding and the use of light and improved the resolving power of the microscope to 200 times.

<p>Antony van Leeuwenhoek (B)</p> Signup and view all the answers

______ is the process of using microorganisms to clean up toxic, hazardous, or unmanageable compounds by degrading them to harmless compounds.

<p>Bioremediation (D)</p> Signup and view all the answers

Flashcards

What are the three domains of life?

The three domains of life are Bacteria, Archaea, and Eukarya.

What are pili?

Pili, also known as fimbriae, are short, hair-like appendages found on the surface of bacteria. They help bacteria adhere to surfaces and other cells.

What is FALSE about flagella?

Flagella are long, whip-like structures used for bacterial movement. While present in bacteria, they are not exclusive to Gram-positive bacteria, meaning they can also be found in Gram-negative bacteria.

What are prions?

Prions are infectious agents that lack a nucleic acid genome (DNA or RNA). They are composed solely of misfolded proteins that can cause other proteins to misfold, leading to disease.

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What are archaea?

Archaea are prokaryotic organisms that have a cell wall but lack peptidoglycan, a unique component of bacterial cell walls. They can thrive in extreme environments like hot springs and salty lakes.

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What produces antibodies?

Antibodies are proteins produced by plasma cells, which are specialized white blood cells. Antibodies help the immune system identify and neutralize foreign invaders like bacteria and viruses.

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What is synergism?

Synergism occurs when two antibiotics work together to enhance their therapeutic effect, meaning the combined effect is greater than the sum of their individual effects.

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Which phase of the growth curve is used to calculate doubling time?

The log phase of bacterial growth is the ideal stage for calculating the doubling time, also known as generation time. This is the time it takes for a bacterial population to double in size.

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How many Biosafety Levels are there?

There are four Biosafety Levels (BSLs) that classify laboratories based on the level of containment required for handling infectious agents. BSL-1 is for the lowest risk, while BSL-4 is for the highest risk.

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Who improved the resolving power of microscopes?

Antony van Leeuwenhoek, a skilled lens grinder, revolutionized microscopy in the 17th century by improving the resolving power of microscopes to 200 times, allowing him to observe tiny organisms for the first time.

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What type of microscope uses visible light?

Light microscopes use visible light to illuminate and magnify specimens. They are commonly used in biology and medicine to view cells, microorganisms, and tissues.

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What is one of Koch's postulates?

Koch's postulates are a set of criteria used to establish a causal relationship between a microorganism and a disease. The second postulate states that the microbe must be isolated and grown in pure culture outside of the host, which is a critical step in proving its role in the disease.

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What is bioremediation?

Bioremediation is a process that uses microorganisms to clean up harmful pollutants in the environment. These microorganisms break down the pollutants into harmless substances.

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What are biofilms?

Biofilms are complex, structured communities of microorganisms attached to a surface. These communities are embedded in a matrix of extracellular polymeric substances (EPS) that provide protection and support.

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What is microbiota?

The term microbiota refers to all the microorganisms that inhabit a particular region, such as the human gut or the soil. Unlike the microbiome, which refers to the collective genetic material of the microbiota, it specifically focuses on the diverse communities of microbes.

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What are methods of reproduction?

Microorganisms reproduce through different methods like cell division, binary fission, mitosis, and meiosis. Binary fission is the most common method of reproduction in prokaryotes and involves the splitting of a parent cell into two daughter cells.

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What is the term for bacteria with flagella at both ends?

Bacteria with flagellum at both ends are called amphitrichous. They have a flagellum at each end of the cell, enabling them to move in both directions.

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What is chitin?

Chitin is a tough, nitrogen-containing polysaccharide found in the cell walls of fungi, some algae, and the exoskeletons of arthropods. It provides structural support and protection.

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Compare prokaryotes and eukaryotes.

Prokaryotes lack a nucleus and other membrane-bound organelles, while eukaryotes have a nucleus and other organelles enclosed within membranes. Prokaryotes are generally smaller and simpler than eukaryotes.

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What are the different types of microscopes?

There are different types of microscopes: light microscopy, electron microscopy (TEM and SEM), and fluorescence microscopy. Each technique utilizes different methods and principles to visualize specimens at varying magnifications and resolutions.

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Compare gram-positive and gram-negative bacteria.

Gram-positive bacteria have a thick peptidoglycan layer in their cell wall, which retains the Gram stain and appears purple under a microscope. Gram-negative bacteria have a thin peptidoglycan layer and an outer membrane, which does not retain the stain and appears pink.

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Name 2 types of bacterial cell morphology and name a bacterium with that morphology.

There are various types of bacterial cell morphology: cocci (spherical), bacilli (rod-shaped), and spirochetes (spiral-shaped). For example, Staphylococcus aureus is a cocci-shaped bacterium.

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Compare enveloped and non-enveloped viruses.

Enveloped viruses have a lipid membrane surrounding their protein capsid, which helps them attach to and enter host cells. Non-enveloped viruses lack this membrane and rely on other mechanisms for entry.

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Name an opportunistic fungal infection and describe in what patient groups it might cause disease?

Candidiasis, a fungal infection caused by Candida albicans, is an opportunistic infection that can affect individuals with weakened immune systems, such as those undergoing chemotherapy or with HIV/AIDS.

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Compare and contrast general and selective growth media.

General growth media supports the growth of a wide range of microorganisms, while selective growth media contains components that inhibit the growth of certain microorganisms while allowing others to grow.

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What is the generation time?

The generation time, also known as doubling time, refers to the time it takes for a bacterial population to double in size. This is a critical parameter for understanding bacterial growth and is influenced by environmental conditions.

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What are aseptic techniques?

Aseptic techniques are essential practices in microbiology labs to prevent contamination. These techniques help minimize the risk of introducing unwanted microbes during experiments, ensuring accurate results.

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What factors influence microbial growth?

Microbial growth is influenced by various factors, including temperature, pH, oxygen availability, and nutrient availability. Each microorganism has an optimal range of these factors for growth.

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What are antibiotics?

Antibiotics are substances produced by microbes or synthesized chemically that inhibit the growth of or kill other microbes. They are widely used to treat bacterial infections.

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What is antimicrobial resistance?

Microbial resistance to antibiotics is a significant public health issue. It occurs when microbes develop the ability to survive and reproduce in the presence of antibiotics, making infections more difficult to treat.

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What is a microscope?

A device used to magnify and view microscopic organisms.

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What are prokaryotes?

Single-celled organisms lacking a membrane-bound nucleus.

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What is a disease?

A condition caused by a microorganism that disrupts normal bodily functions.

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What is an antiseptic?

A chemical used to kill or inhibit the growth of microorganisms.

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What is abiogenesis?

The spontaneous generation of life from non-living matter.

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What is a fluorescence microscope?

A type of microscope that uses fluorescence to illuminate and visualize specimens.

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What is confocal microscopy?

A type of microscopy that uses lasers to illuminate and create three-dimensional images.

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What is parasitism?

A type of interaction where one organism benefits while the other is harmed.

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What are bacteria?

A group of microorganisms that lack organelles and have a simple cellular structure.

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What is biotechnology?

The use of microbes to produce food, pharmaceuticals, or other products.

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What is penicillin?

A substance produced by a fungus that inhibits the growth of bacteria.

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What is taxonomy?

The classification of organisms based on their evolutionary relationships.

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What is vaccination?

The process where one or more organisms are inoculated with a weakened or inactive form of a pathogen to stimulate an immune response.

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What is cholera?

A waterborne disease caused by the bacteria Vibrio cholerae.

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What are animalcules?

Tiny organisms originally observed by van Leeuwenhoek and later named 'animalcules'.

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What is UV sterilization?

A method used to sterilize and disinfect surfaces by using ultraviolet radiation.

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What is a light microscope?

A type of microscope that uses light and lenses to magnify objects up to 1000 times.

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Confocal Microscopy

A type of microscopy that uses lasers to scan a specimen, producing sharp, three-dimensional images.

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Abiogenesis

The spontaneous generation of life from non-living matter. This theory has now been disproven.

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Antiseptic

A chemical agent that kills or inhibits the growth of microorganisms on living tissue.

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Fluorescence Microscopy

A type of light microscopy that uses specific wavelengths of light to illuminate specimens, allowing researchers to visualize specific structures or molecules.

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Parasitism

An interaction between two organisms where one benefits at the expense of the other. One organism is the parasite, the other is the host.

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Microscope

An instrument used to magnify and observe microscopic organisms.

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Bioremediation

The use of microorganisms to clean up waste and industrial pollution.

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Prokaryotes

A group of microorganisms that lack a membrane-bound nucleus and other organelles.

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Cholera

A waterborne disease caused by the bacteria Vibrio cholerae, characterized by severe diarrhea.

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Infectious Disease

Any disease caused by a microorganism.

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Penicillin

A substance produced by a fungus (Penicillium) that inhibits the growth of bacteria.

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Light Microscopy

A type of microscopy where the light waves pass through a specimen, producing an image.

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Taxonomy

The classification of organisms based on their evolutionary relationships.

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Animalcules

Organisms originally named by van Leeuwenhoek, now known as microorganisms.

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Vaccination

A process of introducing a weakened or inactive form of a pathogen into a host to stimulate an immune response.

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Archaea

A domain of life that includes prokaryotes that can live in extreme environments.

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Biotechnology

The use of microbes to produce food, pharmaceuticals, or other products.

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Bacteria

A single-celled organism that lacks membrane-bound organelles and has a simple cellular structure.

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UV Microscopy

A type of microscopy that uses ultraviolet (UV) light to illuminate specimens, producing fluorescent images.

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Virus

A virus is an obligate intracellular parasite. It depends on a host cell to replicate.

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Study Notes

Multiple Choice Questions

  • Domains of Life: Bacteria, archaea, and eukarya
  • Pili: Also called fimbriae.
  • Flagella: Used for movement; present in bacteria; have a hook (at the end of the filament, anchored to the basal body).
  • Infectious Agents: Viroids — infectious agents without nucleic acid genome; Prions; Virusoids
  • Prokaryotic Organisms with cell walls but without peptidoglycan: Archaea
  • Antibodies: Produced by plasma cells.
  • Synergism: Phenomenon where two antibiotics together increase their therapeutic effect.
  • Bacterial Growth Curve calculations: Doubling time calculated in the log phase.
  • Biosafety levels: Number of these levels (1-4).
  • Koch's Postulates (not complete but an important concept): A set of criteria for proving a microorganism causes a disease. Criteria include presence of microbe in diseased, but not healthy, animals; isolating microbe from diseased animal; causing same disease in healthy animal when exposed to microbe; and reisolating the same microbe from experimental animal.
  • Microbial communities Colonies, biofilms, and biospheres.

Microbiology Exam Short Answer Questions

  • Prokaryotes vs. Eukaryotes: Comparison of these cell types for a study session.
  • Types of Microscopes: Different types including light, electron, and compound microscopes. (light microscopy details given).
  • Gram-Positive and Gram-Negative Bacteria: Comparisons for comprehensive study.
  • Bacterial Cell Morphology types: Two types (example) with examples of bacteria.
  • Enveloped and Non-enveloped Viruses: Comparison for study session.
  • Opportunistic Fungal Infections: Examples in specific patient groups.
  • Growth Media types: Examples of selective and general growth media.
  • Microremediation: The process of using microorganisms to clean up toxic compounds.
  • Bioremediation: (related to above).
  • Microbial Communities: (related to biofilms): Examples of colonies, biofilms, and biospheres.
  • Microbiology Methods: (examples given) - use of microscopes and improvements to resolution (e.g., Robert Hooke, Antony van Leeuwenhoek, Louis Pasteur).

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