Microbiology Equipment Overview
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Questions and Answers

What should be applied to the slide before converting to 100x power?

  • Immersion oil (correct)
  • Glycerin
  • Alcohol
  • Water
  • It is acceptable to use coarse adjustment while positioning the slide for oil immersion.

    False (B)

    What is the primary purpose of using immersion oil with the oil lenses?

    To increase the resolving power of the microscope by matching the diffraction factor of oil and glass.

    Before putting a drop of oil, do not move the slide from its __________.

    <p>position</p> Signup and view all the answers

    Match the practices with their corresponding guidelines when using oil immersion lenses:

    <p>Put a drop of oil = Before moving the slide Use coarse adjustment = During oil immersion Apply oil = At the point of light density Move the mechanical stage = With fine adjustment only</p> Signup and view all the answers

    What temperature and pressure are used in an autoclave for sterilizing cultured media?

    <p>121°C at 1 atm for 30 minutes (C)</p> Signup and view all the answers

    An oven operates at a low temperature of 90 minutes for dry heat sterilization.

    <p>False (B)</p> Signup and view all the answers

    What is the main purpose of a biosafety cabinet?

    <p>To provide a safe workspace for handling materials contaminated with pathogens.</p> Signup and view all the answers

    A __________ is used to maintain microbial cultures under optimal conditions.

    <p>incubator</p> Signup and view all the answers

    Match the following laboratory equipment with their primary function:

    <p>Autoclave = Wet heat sterilization Centrifuge = Applies centrifugal force Waterbath = Incubates samples in heated water Refrigerator = Preserves bacterial culture</p> Signup and view all the answers

    Flashcards

    What is an autoclave used for?

    A machine that uses high heat and pressure to sterilize materials by denaturing proteins.

    What is an oven used for in microbiology?

    A device that uses high temperatures (180°C) to sterilize dry materials by oxidation.

    What is an incubator used for?

    A machine that controls the temperature and humidity required to grow and maintain cultures.

    What is a centrifuge used for?

    A machine that spins samples at high speeds, separating them based on density.

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    What is a biosafety cabinet used for?

    A specialized workspace that provides a barrier between the user and potentially hazardous materials.

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    Resolving Power

    The ability of a microscope to distinguish two closely spaced objects as separate entities. It is determined by the wavelength of light used and the numerical aperture of the objective lens.

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    Immersion Oil

    A viscous liquid, typically an oil, that is applied to the slide and lens at high magnification (100x) to enhance light passage and improve image quality.

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    Refraction

    The bending of light as it passes from one medium to another. This effect can distort the image.

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    Oil Immersion Lens

    A specialized lens designed to be used with immersion oil, offering higher magnification and resolving power.

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    Diffraction Factor

    The property of a substance that determines how much light it bends. This value is similar for oil and glass, enabling better clarity and clarity at high magnification.

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    Study Notes

    Equipment

    • Autoclave:

      • Used for wet sterilization—death by protein denaturation.
      • High temperature (121°C).
      • High pressure (1 atm (15 pound/inch)) used to sterilize media (with sugar for 10 min) and uncultured glass ware for 15 min.
    • Oven:

      • Used for dry heat sterilization—death by oxidation.
      • High temperature only (180°C) for 90 min.
      • Used to sterilize some of metallic tools and glass wares.
    • Incubator:

      • A device used to grow and maintain microbiological cultures or cell cultures.
      • The incubator maintains optimal temperature, humidity, and other conditions such as carbon dioxide (CO2) and oxygen content of the atmosphere inside.
    • Refrigerator:

      • Used to maintain sterilized media and broth when not used to avoid the contamination, and also to preserve the bacterial culture for long time by preventing the growth at 4°C.
    • Biological Safety Cabinet (BSC):

      • Also called a biological safety cabinet or microbiological safety cabinet.
      • Is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with (or potentially contaminated with) pathogens.
    • Water Bath:

      • Is laboratory equipment made from a container filled with heated water.
      • It is used to incubate samples in water at a constant temperature over a long period of time.
    • Centrifuge:

      • A machine with a rapidly rotating container that applies centrifugal force to its contents.

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    Description

    Explore the essential equipment used in microbiology for sterilization and culture maintenance. This quiz covers the autoclave, oven, incubator, refrigerator, and biological safety cabinet, detailing their functions and applications in the laboratory. Test your understanding of the role each piece of equipment plays in microbiological practices.

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