Podcast
Questions and Answers
What should be applied to the slide before converting to 100x power?
What should be applied to the slide before converting to 100x power?
It is acceptable to use coarse adjustment while positioning the slide for oil immersion.
It is acceptable to use coarse adjustment while positioning the slide for oil immersion.
False
What is the primary purpose of using immersion oil with the oil lenses?
What is the primary purpose of using immersion oil with the oil lenses?
To increase the resolving power of the microscope by matching the diffraction factor of oil and glass.
Before putting a drop of oil, do not move the slide from its __________.
Before putting a drop of oil, do not move the slide from its __________.
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Match the practices with their corresponding guidelines when using oil immersion lenses:
Match the practices with their corresponding guidelines when using oil immersion lenses:
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What temperature and pressure are used in an autoclave for sterilizing cultured media?
What temperature and pressure are used in an autoclave for sterilizing cultured media?
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An oven operates at a low temperature of 90 minutes for dry heat sterilization.
An oven operates at a low temperature of 90 minutes for dry heat sterilization.
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What is the main purpose of a biosafety cabinet?
What is the main purpose of a biosafety cabinet?
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A __________ is used to maintain microbial cultures under optimal conditions.
A __________ is used to maintain microbial cultures under optimal conditions.
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Match the following laboratory equipment with their primary function:
Match the following laboratory equipment with their primary function:
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Study Notes
Equipment
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Autoclave:
- Used for wet sterilization—death by protein denaturation.
- High temperature (121°C).
- High pressure (1 atm (15 pound/inch)) used to sterilize media (with sugar for 10 min) and uncultured glass ware for 15 min.
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Oven:
- Used for dry heat sterilization—death by oxidation.
- High temperature only (180°C) for 90 min.
- Used to sterilize some of metallic tools and glass wares.
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Incubator:
- A device used to grow and maintain microbiological cultures or cell cultures.
- The incubator maintains optimal temperature, humidity, and other conditions such as carbon dioxide (CO2) and oxygen content of the atmosphere inside.
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Refrigerator:
- Used to maintain sterilized media and broth when not used to avoid the contamination, and also to preserve the bacterial culture for long time by preventing the growth at 4°C.
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Biological Safety Cabinet (BSC):
- Also called a biological safety cabinet or microbiological safety cabinet.
- Is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with (or potentially contaminated with) pathogens.
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Water Bath:
- Is laboratory equipment made from a container filled with heated water.
- It is used to incubate samples in water at a constant temperature over a long period of time.
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Centrifuge:
- A machine with a rapidly rotating container that applies centrifugal force to its contents.
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Description
Explore the essential equipment used in microbiology for sterilization and culture maintenance. This quiz covers the autoclave, oven, incubator, refrigerator, and biological safety cabinet, detailing their functions and applications in the laboratory. Test your understanding of the role each piece of equipment plays in microbiological practices.
