Microbiology: Decontamination and Sterilization Methods

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Questions and Answers

What is the primary agency responsible for regulating chemicals used in sterilization processes?

  • National Institutes of Health (NIH)
  • Food and Drug Administration (FDA) (correct)
  • Center for Disease Control and Prevention (CDC)
  • Environmental Protection Agency (EPA)

Which type of sterilization technique is recommended by the FDA for heat-sensitive materials?

  • Liquid sterilization methods (correct)
  • Gamma radiation sterilization
  • Gaseous techniques
  • UV light sterilization

What is the primary mechanism of action of alcohols in sterilization?

  • Alkylation of amino-, carboxyl- and hydroxyl groups
  • Denaturation of cellular proteins
  • Dehydration of cells, disruption of membranes, and coagulation of protein (correct)
  • Oxidation of essential sulphydryl groups of enzymes

Which of the following is a disadvantage of using alcohols in sterilization?

<p>Skin irritant, volatile, and inflammable (B)</p> Signup and view all the answers

What is the primary mechanism of action of aldehydes in sterilization?

<p>Alkylation of amino-, carboxyl- and hydroxyl groups (D)</p> Signup and view all the answers

Which type of disinfectant is classified based on its spectrum of activity?

<p>All of the above (D)</p> Signup and view all the answers

What is the primary difference between gaseous and liquid sterilization techniques?

<p>Application parameters (A)</p> Signup and view all the answers

Which of the following is an example of a gaseous disinfectant?

<p>Formaldehyde vapor (D)</p> Signup and view all the answers

What is the recommended concentration of aqueous solution for effective sterilization using alcohols?

<p>70% aqueous solution (B)</p> Signup and view all the answers

Which of the following is a classification of disinfectants based on their mechanism of action?

<p>All of the above (D)</p> Signup and view all the answers

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