Podcast
Questions and Answers
What should be done when inoculating to avoid cross-contamination?
What should be done when inoculating to avoid cross-contamination?
- Open plates to ensure they are not contaminated.
- Perform inoculations away from plate 1. (correct)
- Label all plates before inoculation.
- Inoculate plates 1 and 2 simultaneously.
At what temperature should plates 3, 4, 5, 6, and 7 be incubated?
At what temperature should plates 3, 4, 5, 6, and 7 be incubated?
- 20°C
- 25°C
- 30°C
- 37°C (correct)
What information is required when labeling the base of each plate?
What information is required when labeling the base of each plate?
- The cost of materials used.
- The date and type of exposure. (correct)
- The laboratory procedure followed.
- The estimated growth time.
How long should plates be incubated for?
How long should plates be incubated for?
When preparing to observe colonies, what drawing details should be included?
When preparing to observe colonies, what drawing details should be included?
What is the function of Kohler illumination in microscopy?
What is the function of Kohler illumination in microscopy?
What adjustment should be made if the field appears unevenly illuminated?
What adjustment should be made if the field appears unevenly illuminated?
What happens when the field diaphragm is closed?
What happens when the field diaphragm is closed?
How can the light in the field of view be centered?
How can the light in the field of view be centered?
Why is it necessary to adjust the condenser in microscopy?
Why is it necessary to adjust the condenser in microscopy?
How many ocular units correspond to a distance of 1,500 µm?
How many ocular units correspond to a distance of 1,500 µm?
What is the calibration value for one ocular unit (OU) based on the provided information?
What is the calibration value for one ocular unit (OU) based on the provided information?
Which objective lens has a total magnification of 400X?
Which objective lens has a total magnification of 400X?
What should be done if the calculated ocular unit values differ when measuring?
What should be done if the calculated ocular unit values differ when measuring?
Using the information given, what is the distance measurement associated with 25 ocular units?
Using the information given, what is the distance measurement associated with 25 ocular units?
If using the scanning objective at 40X magnification, what is the calibration in µm per ocular unit?
If using the scanning objective at 40X magnification, what is the calibration in µm per ocular unit?
Which total magnification corresponds to the low power objective?
Which total magnification corresponds to the low power objective?
What is the correct relationship between ocular units and the stage micrometer distance?
What is the correct relationship between ocular units and the stage micrometer distance?
How many ocular units does 800 µm span?
How many ocular units does 800 µm span?
Which of the following is true about measuring specimens with an ocular micrometer?
Which of the following is true about measuring specimens with an ocular micrometer?
Flashcards are hidden until you start studying
Study Notes
Inoculation Procedure
- Inoculate away from plate 1 to prevent cross-contamination.
- Plates 7 and 8 should remain covered and not be opened.
- Label the base of each plate with the date, exposure type, and group name or number.
- Invert all plates during incubation for consistency.
Incubation Conditions
- Incubate plates 1, 2, and 8 at 25°C.
- Incubate plates 3, 4, 5, 6, and 7 at 37°C.
- Incubation time ranges from 24 to 48 hours for optimal microbial growth.
Observations and Drawings
- Use provided circles as Petri dishes for drawing colony observations.
- Choose two different colonies from each plate for detailed illustrations.
- Label drawings with incubation time, temperature, and inoculum source, including colony color and abundance.
Microscope Techniques
- Kohler illumination improves uniform lighting when focusing on specimens.
- The field diaphragm can be adjusted to enhance light quality for clearer visibility.
- Adjust condenser height and centering for optimal field diaphragm visibility.
Ocular Micrometer Calibration
- Total magnifications vary across objectives: Scanning (40X), Low Power (100X), High-Dry Power (400X), Oil Immersion (1000X).
- Use ocular micrometer to measure specimens by calculating ocular unit values based on stage micrometer measurements.
Bacterial Emulsion Preparation
- Prepare smears of different organisms on slides without mixing to avoid cross-contamination.
- Heat-fix slides by passing through a flame but avoid overheating to minimize aerosol production.
- Follow staining protocols for consistent results, with specific times for crystal violet and safranin.
Safety and Disposal Practices
- Dispose of used pipette and emulsion slides in designated disinfectant jars or sharps containers to ensure biohazard safety.
- Use sterile tools, such as inoculating needles, especially for BSL-2 organisms, and properly disinfect after use.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
